Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,474,914	Δ1 bp	100%	intergenic (‑77/+49)	RCAP_rcc02301 ← / ← engA	hypothetical protein/GTP‑binding protein EngA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,474,914	0	G→.	100.0%	106.2	32	intergenic (‑77/+49)	RCAP_rcc02301/engA	hypothetical protein/GTP‑binding protein EngA
Reads supporting (aligned to +/- strand):  new base (27/5):  ref base (0/0):  total (27/5)

CTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCC..CC.GCGGGTCGC.CTCGGGCTTGCCCGAGGCGACATCAG.CCCTTC.GGTTTCGAGGGCAGGCCCTTGTCCTTGCGTG  >  NC_014034/2474815‑2475003                                                                                                    |                                                                                                cTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgg                                                                                                <  1:1828798/101‑1 (MQ=255)  tCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggg                                                                                               <  2:4216269/101‑1 (MQ=255)  tCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggg                                                                                               >  1:3007276/1‑101 (MQ=255)   cccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggg                                                                                              <  2:1332699/101‑1 (MQ=255)    ccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggg                                                                                             >  1:3005673/1‑101 (MQ=255)    ccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggg                                                                                             <  2:5775783/101‑1 (MQ=255)     cccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGACCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggggg                                                                                            >  1:4867009/1‑101 (MQ=255)       cGGGATTTTTCCCCCAAGGTGCCAGGAACCCCCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcg                                                                                          <  1:1470239/101‑3 (MQ=255)       cGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCACATACGAAAGGCGGGGGGTGCggggggcg                                                                                          >  1:424974/1‑99 (MQ=255)       cGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcg                                                                                          <  1:92952/101‑3 (MQ=255)       cGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcg                                                                                          <  2:5241187/101‑3 (MQ=255)       cGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcg                                                                                          <  1:645279/101‑3 (MQ=255)        gggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgc                                                                                         <  2:1722786/101‑4 (MQ=255)        gggATATTTCCACCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgc                                                                                         <  2:3557732/101‑4 (MQ=255)         ggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgca                                                                                        >  1:1405470/1‑97 (MQ=255)         ggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgca                                                                                        >  1:5233496/1‑97 (MQ=255)         ggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgca                                                                                        <  1:3508751/101‑5 (MQ=255)         ggATATTTCCCCCAAGGTCGCAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgcc                                                                                        <  1:2281278/101‑5 (MQ=255)           atatTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggggccgc                                                                                      >  1:2354129/1‑96 (MQ=255)            tatTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcc                                                                                    <  1:423927/101‑1 (MQ=255)            tatTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcc                                                                                    <  2:1363442/101‑1 (MQ=255)            tatTTCCCCCAAGGCGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcc                                                                                    <  1:4996339/101‑1 (MQ=255)              tttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc                                                                                  <  1:2361518/101‑1 (MQ=255)               ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc..c                                                                               <  1:3252553/101‑1 (MQ=255)               ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc..c                                                                               >  2:1015646/1‑101 (MQ=255)                    ccAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCgg                                                                         >  1:5400916/1‑101 (MQ=255)                          tGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCggggcccc                                                                   >  1:1892256/1‑96 (MQ=255)                          tGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCggcggggc                                                                   >  1:4592870/1‑95 (MQ=255)                           gACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCGc.cc                                                                 >  2:724407/1‑100 (MQ=255)                           gACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGCCGc.cc                                                                 >  1:4739182/1‑100 (MQ=255)                                gAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCGC.CCCCGGc                                                            >  1:4602472/1‑101 (MQ=37)                                       cgGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGTCGCTCTCGGGCTTGccc                                                      >  2:2562702/1‑101 (MQ=255)                                           cccccccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCGC.CCCGGGCGTGcccccggc                                                 >  1:2227025/1‑96 (MQ=25)                                             cccccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGGGC.CTCGGGCCTGCCCGCGGCgc                                               >  2:1642721/1‑100 (MQ=21)                                             cccccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCCC.CCCGGGCCTGCCCGGggggg                                               >  1:1567489/1‑98 (MQ=21)                                                     cGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCGC.CCCCGGCGTGCCCGCGGCGAcctacacc                                       >  2:5403922/1‑94 (MQ=21)                                                      gAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCGC.CCCCGGCCTGCCCGCGGCGGCATCAG.ccc                                     >  1:428127/1‑101 (MQ=21)                                                           gCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGTGGG.CCCGGGGGTGCCCCAGGGGGCAACAG.CCCCTccg                                >  1:2177770/1‑99 (MQ=18)                                                             ccGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGTCGC.CCCGGGCCTGCCCCGGGGGGCATCAG.GCCCTC.gggt                             >  1:753478/1‑99 (MQ=18)                                                             ccGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCGC.CCCGGGCTTGCCCGGGGGGGCATCAG.CCCCTC.CGtt                             >  1:1296236/1‑101 (MQ=21)                                                                  ttttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGTCGG.CTCCGGCTTGCCCGCGGCGGCGTCAG.GCCCTC.GGGTTCGAg                        >  1:4436688/1‑101 (MQ=18)                                                                  ttttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGCCGC.CCCCGGGCTGCCCCAGGCGGCATCTG.CCCCCC.GGTTTctcg                        >  1:2477905/1‑98 (MQ=16)                                                                   tttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGTCGC.CTCCGGCGTGCCCGCGACGGCCACAG.CCCCTC.TGTTTctggg                       >  1:4659490/1‑97 (MQ=17)                                                                    ttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCGC.CCCGGGGTTTCCCGGGGGGGGCTCAG.CCCTTT.GGGTTCggagg                      >  2:2657517/1‑97 (MQ=16)                                                                            ccAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGTCGC.CTCGGGCTTGCCCGAGGCGACATCAA.CCCTTC.GGGTTCGAGGGCAGGCCCt              >  2:973182/1‑101 (MQ=255)                                                                             cAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCGC.CTCGGGCTTGCCCGAGGCGACATCAG.CCCTTC.GGGTTCGCGGGGAGGCCCtt             >  1:2585933/1‑101 (MQ=21)                                                                              aaaTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCGC.CCCCGGGTTGCCCCGGGGGGCAACCGCCCCTTC.GGTTTGGGGGGGGGGCCCtt             >  1:3160931/1‑101 (MQ=9)                                                                               aaTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGTCGC.CTCGGGCCTGCCCGAGGCGACATCCG.CCCTTC.GGGTTCGAGGGCAGGGCCTTGt           >  2:3472537/1‑101 (MQ=18)                                                                               aaTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCCC.CCCCGGGCTGCCCCACGCCAGCCCAG.CCCCTCGGGTTTCGCGGGC.GGCCCtatt           >  2:1294111/1‑98 (MQ=9)                                                                                aTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGTCGC.CTCGGGCTTGCCCCAGGCGCCATCAG.CCCCTC.GGGTTCGGGGGCCGGCCCTTGTc          >  1:1744740/1‑101 (MQ=18)                                                                                 tACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC..CC.GCGGGGCGC.CCCGGGCTTGCCCGGGGGGGCATCAG.CCCTTC.CGGTTCTGGGGCAGGCCCCTGTcc         >  1:3330595/1‑101 (MQ=18)                                                                                   ggggggggggggggGGCGGGGGCGCGCCCCCCCCGCCGGCGGGTCGC.CTCGGGCTTGCCCGAGGCGACATCAG.CCCTTC.GGTTTCGAGGGCAGGCCCTTGt           <  2:4687181/93‑1 (MQ=21)                                                                                         ggggggggGGGGGGCGGCCGCCTCCCC..CG.GCGGGTCGC.CTCGGGCTTGCCCGAGGCGACATCAG.CCCTTC.GGTTTCGAGGGCAGGCCCTTGTCCTtgcgtg  <  2:2210198/99‑1 (MQ=37)                                                                                                    |                                                                                                CTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCC..CC.GCGGGTCGC.CTCGGGCTTGCCCGAGGCGACATCAG.CCCTTC.GGTTTCGAGGGCAGGCCCTTGTCCTTGCGTG  >  NC_014034/2474815‑2475003 Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG