BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,876,588	Δ1 bp	2.5%	intergenic (+87/‑106)	RCAP_rcc02685 → / → prfA	glutathione S‑transferase/peptide chain release factor 1

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,876,588	0	G→.	2.5%	4.8 ‑3.6 ‑0.1	157	intergenic (+87/‑106)	RCAP_rcc02685/prfA	glutathione S‑transferase/peptide chain release factor 1
Reads supporting (aligned to +/- strand): new base (4/0): ref base (61/92): total (65/92)
Fisher's exact test strand distribution p-value = 2.78e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.70e-01

Only 100 of 184 total aligned reads displayed.


CCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATCCCCGCCCACCCCCGCCCACCCTTGACCCAGAGCCCGGTCTCGGTCAGAT  >  NC_014034/2876488‑2876687


ccGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGacgacg                                                                                                            <  1:1894616/101‑1 (MQ=255)


ccGAACCGGCGTGACCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGacgacg                                                                                                            <  2:2616736/101‑1 (MQ=255)


 cGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACgg                                                                                                           >  1:2581995/1‑101 (MQ=255)


 cGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACgg                                                                                                           <  1:252666/101‑1 (MQ=255)


  gAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACggg                                                                                                          <  1:469982/101‑1 (MQ=255)


   aaCCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGCCgggg                                                                                                         >  1:4172448/1‑101 (MQ=255)


   aaCCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACgggg                                                                                                         <  2:2399630/101‑1 (MQ=255)


   aaCCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACgggg                                                                                                         <  2:3481575/101‑1 (MQ=255)


   aaCCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACgggg                                                                                                         <  1:3685561/101‑1 (MQ=255)


    aCCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGc                                                                                                        <  1:1477995/101‑1 (MQ=255)


     ccGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCa                                                                                                       >  1:5365433/1‑101 (MQ=255)


     ccGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCa                                                                                                       <  1:1901243/101‑1 (MQ=255)


      cGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAg                                                                                                      <  1:2141732/101‑1 (MQ=255)


       ggCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAgc                                                                                                     >  1:3179836/1‑101 (MQ=255)


       ggCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAgc                                                                                                     >  2:4934521/1‑101 (MQ=255)


       ggCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAgc                                                                                                     >  1:1369539/1‑101 (MQ=255)


       ggCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAgc                                                                                                     <  2:94425/101‑1 (MQ=255)


       ggCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAACACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAgc                                                                                                     <  2:256036/101‑1 (MQ=255)


       ggCGTGATCCCCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGACGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAgc                                                                                                     <  1:4869877/101‑1 (MQ=255)


        tcttGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAgcg                                                                                                    <  2:2257053/98‑1 (MQ=255)


        gCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAgcg                                                                                                    >  2:1477357/1‑101 (MQ=255)


          gTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.cc                                                                                                 <  1:1316527/101‑1 (MQ=255)


           tGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.ccc                                                                                                <  1:1511542/101‑1 (MQ=255)


           tGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.cca                                                                                                >  2:5596077/1‑100 (MQ=255)


            gATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.cccc                                                                                               >  1:4998474/1‑101 (MQ=255)


             aTCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.ccccc                                                                                              <  2:3248652/101‑1 (MQ=255)


             aTCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.ccccc                                                                                              <  2:1885923/101‑1 (MQ=255)


             aTCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.ccccc                                                                                              <  1:1995087/101‑1 (MQ=255)


             aTCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.ccccc                                                                                              <  1:4313460/101‑1 (MQ=255)


              tCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCcccg                                                                                             <  1:5626628/101‑1 (MQ=255)


                 gccgcGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCcccgccc                                                                                          >  1:2700019/1‑101 (MQ=255)


                 gccgcGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCcccgccc                                                                                          <  2:2054083/101‑1 (MQ=255)


                  ccgcGCGCCCCTGCTTTTTCTTGGGGAAAATCCGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCcccgcccg                                                                                         <  1:3992620/101‑1 (MQ=255)


                    gcgcgcCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCggg                                                                                       <  1:227988/101‑1 (MQ=255)


                      gcgcCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTc                                                                                     <  1:1565829/101‑1 (MQ=255)


                      gcgcCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTc                                                                                     <  2:2792057/101‑1 (MQ=255)


                        gcCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCATCG.CCCCCGCCCGGGTCG.c                                                                                  <  2:3718290/101‑1 (MQ=255)


                        gcCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.c                                                                                  >  2:1187739/1‑101 (MQ=255)


                         ccccTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.cc                                                                                 >  2:1399769/1‑101 (MQ=255)


                          cccTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.ccc                                                                                >  1:4154959/1‑101 (MQ=255)


                           ccTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.cccc                                                                               <  1:38431/101‑1 (MQ=255)


                            cTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCg                                                                              <  1:925076/101‑1 (MQ=255)


                             tGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCNCGCCCGCGTCG.CCCCgg                                                                             >  2:2819740/1‑101 (MQ=255)


                              gCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCggg                                                                            >  1:4776436/1‑101 (MQ=255)


                               cTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGgc                                                                           <  2:4229924/101‑1 (MQ=255)


                               cTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.CCCCcggg                                                                           >  1:4641579/1‑100 (MQ=38)


                                tttttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.CCCCcgggg                                                                          >  1:5428641/1‑99 (MQ=37)


                                tttttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.CCCCcgggc                                                                          >  2:5075458/1‑99 (MQ=37)


                                 ttttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCGGGGCG.CCCCGGgcg.c                                                                        >  1:3794793/1‑101 (MQ=255)


                                 ttttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.CCCCCGgccc                                                                         >  2:4578010/1‑99 (MQ=25)


                                 ttttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGACC.CCCCGGggg.c                                                                        >  2:807940/1‑101 (MQ=25)


                                 ttttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCCATGGACGACGGGGCAGCG.CCCCCCCCCGGGGCG.CCCCGGgcg.c                                                                        >  1:571366/1‑101 (MQ=255)


                                    tCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.c                                                                   <  1:2819901/101‑1 (MQ=255)


                                      tgggggAAAATGCGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGAGGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.ccc                                                                 <  2:2091584/99‑1 (MQ=255)


                                       tGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.ccc.c                                                               <  2:1031127/101‑1 (MQ=255)


                                       tGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCGGGGCG.CCCCGGGGG.C.CG.ccc.c                                                               >  1:3428130/1‑101 (MQ=25)


                                        ggggAAAATACGCCGGGGGTCGTCCATTGCGTCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.GCCCCGCCCCGGGTG.CGCCGGggg.ctcacccc                                                                 >  1:5178535/1‑94 (MQ=25)


                                        ggggAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.GCCCCGGCG.C.CG.GCC.C.g                                                             >  2:3988329/1‑101 (MQ=25)


                                         gggAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.gg                                                            <  2:4444774/101‑1 (MQ=255)


                                          ggAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.ggg                                                           <  1:1844598/101‑1 (MQ=255)


                                           gAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGGCG.CCCCCGGCG.C.CG.CCC.C.cggg                                                          >  2:5306677/1‑101 (MQ=37)


                                            aaaaTACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGc                                                         <  1:2598335/101‑1 (MQ=255)


                                              aaTACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCGCCCCCCCCCCGGGCGG.CCCCCGGCG.C.CG.CCC.C.gcgggc                                                        >  1:5063921/1‑99 (MQ=25)


                                               attcGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGaa                                                      <  1:1248088/98‑1 (MQ=255)


                                                 tcccggggggTCGTCCATTGGGGCACCATCGGCTCAAGACCCGATGGACGAC.GGGCGGAG.CCCCCG.GCGGGGCG.CCCCGGGCGCC.AG.CCC.C.CGGGGGaaac                                                    >  1:4236871/6‑100 (MQ=25)


                                                 aCGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAAt                                                    <  2:3050016/101‑1 (MQ=255)


                                                  cGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATc                                                   <  2:391133/101‑1 (MQ=255)


                                                  cGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATc                                                   <  1:1142414/101‑1 (MQ=255)


                                                   gCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.CCCCCGGCG.C.CGCCCC.C.GGGGGGaaacc                                                   >  1:1155706/1‑99 (MQ=21)


                                                   ccggggggTCGTCCATTGGGGCACCATCGGCTCAAGACCCGATGGACGAC.GGGCGGAG.CCCCCGGCGGGGGGC.CCCCGGGGC.C.AG.CCCGC.GGGGcaatacc                                                   >  1:5503536/4‑95 (MQ=21)


                                                    ccGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccc                                                 <  1:151937/101‑1 (MQ=255)


                                                     ggggggTCGTCCATTGGGGCACCATCGGCTCAAGACCCGATGGACGAC.GGGCGGAG.CCCCCG.GCGGGGCG.CCCCGGGGG.CAAG.CCC.C.CGGGGGAAACccaa                                                >  1:2437483/2‑99 (MQ=21)


                                                      gggggTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccccg                                               <  1:5560448/101‑1 (MQ=255)


                                                      gggggTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGGCG.CCCCGGGCG.C.CG.CCC.C.GGGGGGGAAAccccc                                               >  2:3248690/1‑100 (MQ=25)


                                                          gtcgtcCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccccgccca                                           <  2:2458069/101‑1 (MQ=255)


                                                          gtagtcCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccccgccca                                           <  2:886014/98‑1 (MQ=255)


                                                            cgtcCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCCGGGCG.CCCCCGGCG.C.CGCCCC.C.GGGGGGAAACcccccccccc                                          >  2:2940275/1‑99 (MQ=25)


                                                             gtcCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccccgcccaccc                                        <  1:3362813/101‑1 (MQ=255)


                                                             gtcCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.CCCCCGGGG.C.CG.CCC.C.CGGGGGGAAAcccccccccccc                                        >  1:2047570/1‑101 (MQ=21)


                                                               ccATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccccgcccaccccc                                      <  2:4003374/101‑1 (MQ=255)


                                                               ccATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.CCCCCGGCG.C.CG.CCC.C.CGGGCGGAAAcccccccccccccc                                      >  1:3581344/1‑101 (MQ=255)


                                                               ccATTGCGGCGCCATCGGGTCAAG.CCCGATGGACGCCGGGGCAGCG.CCCCCCCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccccgcccaccccc                                      <  1:3982778/101‑1 (MQ=255)


                                                                cATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGGCG.CCCCCGGCG.C.CG.CCC.C.GGGGGGGAAAcaccgcccccccccc                                     >  2:1712063/1‑100 (MQ=37)


                                                                  ttGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccccgcccacccccgcc                                   <  2:5766332/101‑1 (MQ=255)


                                                                   tGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccccgcccacccccgccc                                  <  1:589849/101‑1 (MQ=255)


                                                                   tGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCAAAATccccgcccacccccgccc                                  <  2:1254912/101‑1 (MQ=255)


                                                                    gcggcgCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCTAAATccccgcccacccccgccca                                 <  2:1866237/101‑1 (MQ=255)


                                                                     cggcgCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccccgcccacccccgcccac                                <  2:5007920/101‑1 (MQ=255)


                                                                     cggcgCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATccccgcccacccccgcccac                                <  1:5338161/101‑1 (MQ=255)


                                                                                gTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATCCCCGCCCACCCCCGCCCACCCTTGACCCag                     <  1:4812715/101‑1 (MQ=255)


                                                                                 ttCAAG.ACCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATCCCCGCCCACCCCCGCCCACCCTTGACCCaga                    <  2:3946581/101‑1 (MQ=255)


                                                                                  tCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATCCCCGCCCACCCCCGCCCACCCTTGACCCagag                   <  2:5428351/101‑1 (MQ=255)


                                                                                  tCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.CCCCCGGGG.C.CGCCCC.C.GGGGGGAAAACCCCCCCCCCCCCCGCCCACCCTCGCcccgag                    >  2:5197864/1‑98 (MQ=255)


                                                                                        cccGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATCCCCGCCCACCCCCGCCCACCCTTGACCCAGAGCCCgg              <  2:5356934/101‑1 (MQ=255)


                                                                                            aTGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATCCCCGCCCACCCCCGCCCACCCTTGACCCAGAGCCCGGtctc          <  1:4387181/101‑1 (MQ=255)


                                                                                             tGGACGACGGGGCAGCG.CCCCCCCCCCGGGCG.CCCCCGGCG.C.CG.CCC.C.GGGGCGGAATCCCCCCCCCCCCCCCCCCCCCCCTGACCCCCAGCCCGGTCTCg         >  2:5584823/1‑101 (MQ=255)


                                                                                              ggACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATCCCCGCCCACCCCCGCCCACCCTTGACCCAGAGCCCGGTCTCgg        <  2:1756936/101‑1 (MQ=255)


                                                                                              ggACGACGGGGCAGCG.CCCCCGCCCGGGGCGCCCCCGGGGG.C.GC.CCC.C.GGGGGGAAAACCCCCCCCCCCCCCCCCCCCCCTT.ACCCCCAGCCCCGgctccg        >  1:4540077/1‑99 (MQ=255)


                                                                                               gacgacGGGGCAGCG.CCCCCGCCCGGGGCG.CCCCCGGGG.C.CG.CCC.CGGGGGGGAAATCCCCCCCCCCCCCCCCCCCCCCTTAGCCCGAAGCCCgggtccgg        >  2:5704393/1‑95 (MQ=255)


                                                                                                    cgGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATCCCCGCCCACCCCCGCCCACCCTTGACCCAGAGCCCGGTCTCGGTCAGAt  <  1:5596369/101‑1 (MQ=255)


CCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.CCCGATGGACGACGGGGCAGCG.CCCCCGCCCGGGTCG.CCCCGGGCG.C.AG.CCC.C.GGGGCGAAATCCCCGCCCACCCCCGCCCACCCTTGACCCAGAGCCCGGTCTCGGTCAGAT  >  NC_014034/2876488‑2876687

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG