Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,024,766 Δ1 bp 100% intergenic (‑176/‑152) chvI ← / → pckA transcriptional regulator ChvI/phosphoenolpyruvate carboxykinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,024,7660T→.100.0% 1489.8 334intergenic (‑176/‑152)chvI/pckAtranscriptional regulator ChvI/phosphoenolpyruvate carboxykinase
Reads supporting (aligned to +/- strand):  new base (167/167):  ref base (0/0):  total (167/167)

Only 100 of 363 total aligned reads displayed.

TGCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGTTTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTGCCCGCCC  >  NC_014034/3024666‑3024862
                                                                                                    |                                                                                                
tgCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGt                                                                                                  <  1:3197587/101‑1 (MQ=255)
tgCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGt                                                                                                  >  2:2814968/1‑101 (MQ=255)
 gCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtt                                                                                                 <  1:338070/101‑1 (MQ=255)
  cGAATCGCCCACGTGATGCGAATCATNNGNNCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGttt                                                                                                <  1:3797184/101‑1 (MQ=255)
  cGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGttt                                                                                                <  2:3989642/101‑1 (MQ=255)
    aaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgc                                                                                              <  1:359983/101‑3 (MQ=255)
      tCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgcgc                                                                                            >  2:2614255/1‑97 (MQ=255)
       cGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCt                                                                                          >  1:697971/1‑101 (MQ=255)
        gCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTa                                                                                         <  1:2533006/101‑1 (MQ=255)
        gCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTa                                                                                         >  2:5097460/1‑101 (MQ=255)
        gCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTa                                                                                         >  2:4137657/1‑101 (MQ=255)
         cccACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAg                                                                                        <  1:625447/101‑1 (MQ=255)
         cccACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAg                                                                                        >  2:5663843/1‑101 (MQ=255)
           cACGTGATGCGAATCATCTGAGCGCAAAAAAACAAAGGTTTGGCTAATTTTTCTCACCTCTTCGCGGTGATGGCCCTAAATCGGCCCTGTTTT.CGCTAgat                                                                                      >  2:4762449/1‑99 (MQ=255)
              gTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCg                                                                                   >  1:3489051/1‑101 (MQ=255)
              gTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCg                                                                                   >  2:3761448/1‑101 (MQ=255)
               tGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGa                                                                                  >  2:4341957/1‑101 (MQ=255)
               tGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGa                                                                                  <  1:1714396/101‑1 (MQ=255)
               tGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGa                                                                                  >  2:122852/1‑101 (MQ=255)
               tGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGa                                                                                  >  1:1180727/1‑101 (MQ=255)
                gATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGAc                                                                                 >  2:2360387/1‑101 (MQ=255)
                cgcgataaTCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGAc                                                                                 <  2:3308434/95‑1 (MQ=255)
                  tGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACaa                                                                               >  2:4454334/1‑101 (MQ=255)
                   gCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACaaa                                                                              >  2:2098492/1‑101 (MQ=255)
                     gAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAcc                                                                            >  1:5089375/1‑101 (MQ=255)
                     gAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAcc                                                                            <  1:2715566/101‑1 (MQ=255)
                       atcatcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCt                                                                          <  1:5295075/101‑1 (MQ=255)
                       atcatcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCt                                                                          <  1:4081930/101‑1 (MQ=255)
                       atcatcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCt                                                                          <  2:5153313/101‑1 (MQ=255)
                         catcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTc                                                                        >  2:1477940/1‑101 (MQ=255)
                         catcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTc                                                                        >  2:4591295/1‑101 (MQ=255)
                          atcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCGCCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCt                                                                       <  1:5479928/101‑1 (MQ=255)
                          atcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACGTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCt                                                                       <  2:3296175/101‑1 (MQ=255)
                          atcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCt                                                                       >  1:2317844/1‑101 (MQ=255)
                           tcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTg                                                                      >  2:5346188/1‑101 (MQ=255)
                            cTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGGTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGt                                                                     <  2:3877513/101‑1 (MQ=255)
                             tGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGtt                                                                    >  1:4846971/1‑101 (MQ=255)
                              gAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTc                                                                   <  2:804268/101‑1 (MQ=255)
                               aGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTcc                                                                  >  1:2916656/1‑101 (MQ=255)
                               aGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTcc                                                                  <  1:3699718/101‑1 (MQ=255)
                                gcgcAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCt                                                                 >  2:3038140/1‑101 (MQ=255)
                                  gcAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTc                                                               <  2:477815/101‑1 (MQ=255)
                                    aaaaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGc                                                             >  2:5252205/1‑101 (MQ=255)
                                    aaaaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGc                                                             <  2:5464605/101‑1 (MQ=255)
                                      aaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCaa                                                           <  1:2614404/101‑1 (MQ=255)
                                        aaaCGAAGGTTTGACTAATTTGTCTCTCCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAtt                                                         <  2:1807818/101‑1 (MQ=255)
                                          aCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgc                                                       >  1:4648610/1‑101 (MQ=255)
                                          aCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgc                                                       >  1:4232697/1‑101 (MQ=255)
                                           cGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgcg                                                      >  1:5590404/1‑101 (MQ=255)
                                            gAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgcgc                                                     >  2:629762/1‑101 (MQ=255)
                                             aaGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGcc                                                    >  2:776326/1‑101 (MQ=255)
                                               ggTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACACACCCTTCTGTTCCTTCGCAATTGCGCCAt                                                  >  2:1942782/1‑101 (MQ=255)
                                               ggTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCAt                                                  >  2:3155294/1‑101 (MQ=255)
                                               ggTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCAt                                                  <  2:5585157/101‑1 (MQ=255)
                                                gTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATg                                                 <  1:1050235/101‑1 (MQ=255)
                                                gTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATg                                                 <  1:2200222/101‑1 (MQ=255)
                                                 tttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATgg                                                <  1:2403015/101‑1 (MQ=255)
                                                  ttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGt                                               <  1:1837136/101‑1 (MQ=255)
                                                  ttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGt                                               <  1:2162560/101‑1 (MQ=255)
                                                  ttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGt                                               >  1:4748130/1‑101 (MQ=255)
                                                   tGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGta                                              >  1:4122312/1‑101 (MQ=255)
                                                   tGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGta                                              >  2:4082627/1‑101 (MQ=255)
                                                   tGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGta                                              >  2:3939188/1‑101 (MQ=255)
                                                    gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             >  2:965832/1‑101 (MQ=255)
                                                      cTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAg                                           >  1:4918747/1‑101 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                          >  2:2727723/1‑101 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                          >  1:5348944/1‑101 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                          >  2:242619/1‑101 (MQ=255)
                                                        aaTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAggg                                         <  1:4342223/101‑1 (MQ=255)
                                                        aaTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAggg                                         >  1:4617551/1‑101 (MQ=255)
                                                        aaTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCCTCTGTTCCTTCGCAATTGCGCCATGGTATAggg                                         >  1:4981692/1‑101 (MQ=255)
                                                          tttGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgcg                                       <  2:4169066/101‑1 (MQ=255)
                                                           ttGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgcgc                                      >  1:234493/1‑101 (MQ=255)
                                                            tGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAGCCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGcc                                     <  2:1001096/101‑1 (MQ=255)
                                                            tGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGcc                                     <  2:4860852/101‑1 (MQ=255)
                                                                tcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATcc                                 >  2:582749/1‑101 (MQ=255)
                                                                 cACCTCTTCGCGATTATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCg                                >  1:3531623/1‑101 (MQ=255)
                                                                 cACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCg                                >  2:4249386/1‑101 (MQ=255)
                                                                 cACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCg                                >  1:5623285/1‑101 (MQ=255)
                                                                   ccTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCa                              >  2:2483298/1‑101 (MQ=255)
                                                                   ccTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCa                              >  1:5188883/1‑101 (MQ=255)
                                                                   ccTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCa                              <  1:2009643/101‑1 (MQ=255)
                                                                     tctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAAc                            <  2:2916489/101‑1 (MQ=255)
                                                                      ctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACa                           <  2:3588168/101‑1 (MQ=255)
                                                                      ctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACa                           <  2:1299085/101‑1 (MQ=255)
                                                                       ttCGCGATGATGGCGTTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCGTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACAt                          <  2:4620619/101‑1 (MQ=255)
                                                                       ttCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACAt                          >  1:4457037/1‑101 (MQ=255)
                                                                         ggcgATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTc                        >  1:840112/2‑101 (MQ=255)
                                                                           cgATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCtt                      >  2:216737/1‑101 (MQ=255)
                                                                             atgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgc                    >  1:3754360/1‑101 (MQ=255)
                                                                             atgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgc                    <  2:3391722/101‑1 (MQ=255)
                                                                                 tgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCgg                <  1:1391093/101‑1 (MQ=255)
                                                                                   gcgcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGAt              >  1:3008570/1‑101 (MQ=255)
                                                                                   gcgcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGAt              <  1:2107337/101‑1 (MQ=255)
                                                                                        aaaTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTg         >  2:1054021/1‑101 (MQ=255)
                                                                                         aaTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgc        >  1:3351087/1‑101 (MQ=255)
                                                                                          aTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcc       <  2:2275951/101‑1 (MQ=255)
                                                                                           tCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgccc      >  2:140683/1‑101 (MQ=255)
                                                                                           acGGCCCTGTTGGGC.CTAGCTTCGACGATCCTTCCTGTGCCTGTGCTATTGCCGCATGGTGTAGGGGGCCATCCGCAACATTCTTGCGCGGATCGGTgccc      <  2:1344680/100‑1 (MQ=255)
                                                                                              gCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccgcc   >  1:1891625/1‑101 (MQ=255)
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TGCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGTTTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTGCCCGCCC  >  NC_014034/3024666‑3024862

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG