Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,468 Δ1 bp 3.5% intergenic (+71/+77) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4680A→. 3.5% 15.3 ‑1.9 ‑1.0230intergenic (+71/+77)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/2):  ref base (97/124):  total (103/127)
Fisher's exact test strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.07e-01

Only 100 of 260 total aligned reads displayed.

CGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCG  >  NC_014034/432369‑432568
                                                                                                     |                                                                                                     
cGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGa                                                                                                       <  2:3915665/101‑1 (MQ=255)
 gCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCCGGCAATTTGAAGCGAGAAGGAAGGCGaa                                                                                                      >  2:5029853/1‑100 (MQ=18)
     tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCgg                                                                                                  <  2:455278/101‑1 (MQ=255)
     tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCgg                                                                                                  <  2:3724479/101‑1 (MQ=255)
     tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACACCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCgg                                                                                                  <  1:2267225/101‑1 (MQ=255)
      cggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCcgg                                                                                                 >  1:4454289/1‑101 (MQ=255)
        gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                               >  2:192097/1‑101 (MQ=255)
         cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                              >  1:1616403/1‑101 (MQ=255)
         cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                              <  1:995372/101‑1 (MQ=255)
          ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                             <  1:3723534/101‑1 (MQ=255)
           gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                            <  2:4119138/101‑1 (MQ=255)
            gggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                           <  2:4389382/100‑1 (MQ=255)
            cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                           <  1:5505020/101‑1 (MQ=255)
               cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                        <  2:5083619/101‑1 (MQ=255)
                gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCa                                                                                       >  1:2702692/1‑101 (MQ=255)
                 ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                      <  2:59371/101‑1 (MQ=255)
                   ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                                    <  1:5462761/101‑1 (MQ=255)
                    cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                   >  1:4331278/1‑101 (MQ=255)
                    cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCAATCCGGGCTTCGTTTCATCtt                                                                                   <  1:4265961/101‑1 (MQ=255)
                         cTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                              >  2:4084339/1‑101 (MQ=255)
                           gAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaa                                                                            >  1:1304300/1‑101 (MQ=255)
                           gAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCCGGGTATTTGAGGCAAGATGAAAACCGATCCGGGGTTCGTTTCTTTTTTccccca                                                                            >  1:3946796/1‑99 (MQ=17)
                            aGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaaa                                                                           <  2:1015197/101‑1 (MQ=255)
                            aGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaaa                                                                           <  1:3178902/101‑1 (MQ=255)
                               gcGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGTGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                        <  2:3785604/100‑1 (MQ=255)
                                cGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.c                                                                      >  1:2416775/1‑101 (MQ=255)
                                 gggCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGGAATTTGGGTCAAGATGAAAGCCGATGCGGGCTTCGGTTCATCTTGCCCCCAATAA.cc                                                                     >  1:3983753/1‑101 (MQ=18)
                                 gggCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.cc                                                                     >  2:1762848/1‑101 (MQ=255)
                                 gggCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.cc                                                                     <  2:735686/101‑1 (MQ=255)
                                  ggacggggggCTCTGCCCCCC.GG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATAT.CCCAGGGGTGAAtt                                                         >  2:2125221/5‑101 (MQ=2)
                                  ggCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.ccc                                                                    <  1:1872491/101‑1 (MQ=255)
                                    cAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCgg                                                                  >  1:1485863/1‑101 (MQ=255)
                                    acggggggCTCTGCCCCCC.GG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATAT.CCCAGGGGTGAAttgg                                                       >  1:5550287/3‑99 (MQ=2)
                                     cggggggCTCTGCCCCCC.GG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATAT.CCCAGGGGTGAAttggc                                                      >  2:4762476/2‑98 (MQ=2)
                                      ggggggGCCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCgggg                                                                <  1:767491/101‑1 (MQ=25)
                                      ggggggCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCCAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCACATAT.CCCgggg                                                                >  1:466918/1‑101 (MQ=25)
                                      ggggggCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCgggg                                                                >  1:2562656/1‑101 (MQ=255)
                                         gggCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAAGCGATCCGGGCTTCGTTTCATCTTGCCCCCAATAT.CCCcgggggg                                                             >  1:1046620/1‑99 (MQ=21)
                                          ggCTCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGa                                                            <  2:1327564/101‑1 (MQ=255)
                                          ggCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCCAGATGAAAGCCGATCCGGGCTNCGNTNCNTCTTGCCCCCANTAT.CCCGGGGGGGa                                                            >  1:4984814/1‑101 (MQ=21)
                                          ggCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGa                                                            <  1:920166/101‑1 (MQ=255)
                                           gcgcTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGaa                                                           <  2:4454017/101‑1 (MQ=255)
                                           gcgcTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGaa                                                           <  2:4287979/101‑1 (MQ=255)
                                            cgcTGCCCCCCGGC.CTGCGGACTCCCCCCGGGGTATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATTTTTCCCCAAATAA.CCCGGGGGGGAAt                                                          >  1:3141422/1‑101 (MQ=21)
                                             gcTCCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtt                                                         <  2:151496/101‑1 (MQ=255)
                                              ctccccccc.GTGCTGCGGGCAGCCCCCGGGATATTTGAGGAAAAAGGAAAGCCGATCCGGCCTTCGTTTCGTCTTGCCCCAAATGT.CCCGGGGGTGAAttt                                                        <  2:1354628/98‑1 (MQ=21)
                                               tGCCCCCC.GTCCTGCGGACTCCCCCCGGGGTATTTTAGGGAAAATGAAAACCGGACCCGGGTTCGGTTTCTCTTTCCCCCCATATCCCCGGGGGGGAAttt                                                        >  1:45685/1‑101 (MQ=2)
                                               tGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtttt                                                       <  1:5011224/101‑1 (MQ=255)
                                               tGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtttt                                                       >  2:2755537/1‑101 (MQ=255)
                                               tGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtttt                                                       <  2:5474506/101‑1 (MQ=255)
                                               tGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGGGAAtttt                                                       >  1:4643210/1‑101 (MQ=255)
                                                gCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTg                                                      <  2:2364026/101‑1 (MQ=255)
                                                 cccccc.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTgg                                                     >  1:1761281/1‑101 (MQ=255)
                                                   cccc.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGgcg                                                   <  2:5673966/101‑1 (MQ=255)
                                                       cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAg                                                >  1:3345161/5‑101 (MQ=255)
                                                       cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAg                                                <  2:2544425/97‑1 (MQ=255)
                                                       cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAg                                                >  1:3652063/5‑101 (MQ=255)
                                                        gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGc                                               <  2:2874959/98‑1 (MQ=255)
                                                        gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGc                                               <  2:692784/98‑1 (MQ=255)
                                                          ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCa                                             <  2:1797681/100‑1 (MQ=255)
                                                          ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGAGGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCa                                             <  2:3523564/100‑1 (MQ=255)
                                                              cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAag                                         <  1:2041246/101‑1 (MQ=255)
                                                              cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGGGAATTTTGGCGCAGCCAAAag                                         >  1:5604183/1‑101 (MQ=255)
                                                                 aCTCCCCCCGGGATATTTCAGGCAAGATGAAAGGCGATCCGGGCTTGGTTTCATCCTGCCCCAAATAT.CCCGGGGGGGAATTTTGGCGCAGCCAAAAGAgg                                      >  2:5183687/1‑101 (MQ=18)
                                                                    ccccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggggg                                   <  1:4166356/101‑1 (MQ=255)
                                                                      ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                 <  1:3212052/101‑1 (MQ=255)
                                                                      ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                 <  2:3313220/101‑1 (MQ=255)
                                                                       cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                <  2:5216750/101‑1 (MQ=255)
                                                                       cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                >  1:1564226/1‑101 (MQ=255)
                                                                       cccGGGATATCTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                <  2:120476/101‑1 (MQ=255)
                                                                        ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                               <  2:563656/101‑1 (MQ=255)
                                                                        ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                               <  1:2672692/101‑1 (MQ=255)
                                                                         cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                              >  2:4613932/1‑101 (MQ=255)
                                                                         cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                              <  2:3999384/101‑1 (MQ=255)
                                                                          gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                             >  1:2400090/1‑101 (MQ=255)
                                                                             atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                          >  1:669364/1‑101 (MQ=255)
                                                                             atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                          >  1:3424483/1‑101 (MQ=255)
                                                                             atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCAGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                          >  1:2095129/1‑101 (MQ=255)
                                                                              tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                         >  1:248602/1‑101 (MQ=255)
                                                                               atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                        >  1:1663798/1‑101 (MQ=255)
                                                                                tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                       >  2:3131459/1‑101 (MQ=255)
                                                                                 tggAGGAAAGATGAAGGCCGATCCGGGCTGCGTTTCATCTTGCGCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCTCCCCCTAt                      <  2:159711/99‑1 (MQ=21)
                                                                                   gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc                    >  1:538964/1‑101 (MQ=255)
                                                                                    aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTTAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                   <  2:1690052/101‑1 (MQ=255)
                                                                                     ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                  <  1:4493856/101‑1 (MQ=255)
                                                                                      gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                 <  2:245172/101‑1 (MQ=255)
                                                                                        aaGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg               <  1:5561719/101‑1 (MQ=255)
                                                                                         aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg              >  2:3385946/1‑101 (MQ=255)
                                                                                         aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg              >  2:2697940/1‑101 (MQ=255)
                                                                                             gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg          >  1:5083847/1‑101 (MQ=255)
                                                                                             gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg          >  2:2991101/1‑101 (MQ=255)
                                                                                             gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg          <  2:1571886/101‑1 (MQ=255)
                                                                                              aaaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg         >  2:4857436/1‑101 (MQ=255)
                                                                                               aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggccgcgc        >  1:1027197/1‑96 (MQ=255)
                                                                                               aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc        >  1:5770408/1‑101 (MQ=255)
                                                                                                   cGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg    <  2:4949094/101‑1 (MQ=255)
                                                                                                    gATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc   >  1:4496320/1‑101 (MQ=255)
                                                                                                    gATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc   >  1:1369795/1‑101 (MQ=255)
                                                                                                     aTCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg  >  2:3605901/1‑101 (MQ=255)
                                                                                                     aTCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg  >  2:2183646/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCG  >  NC_014034/432369‑432568

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG