Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,200,794 +G 100% intergenic (+3/‑114) trpF → / → trpB1 phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,200,7941.→G100.0% 113.3 212intergenic (+3/‑114)trpF/trpB1phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta
Reads supporting (aligned to +/- strand):  new base (37/139):  ref base (27/9):  total (64/148)
Fisher's exact test strand distribution p-value = 1.05e-09
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.19e-07

Only 100 of 256 total aligned reads displayed.

GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.T.GGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGA  >  NC_014034/1200694‑1200889
                                                                                                        |                                                                                                   
 ggcggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.T.g                                                                                                    <  2:2917607/101‑1 (MQ=255)
  gcggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.T.gg                                                                                                   <  1:4783729/101‑1 (MQ=255)
   cggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCCGGGGGA..GGG.G.ggg                                                                                                  >  1:2700066/1‑101 (MQ=255)
    ggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA..GGG.G.ggg.g                                                                                                >  2:2088417/1‑101 (MQ=255)
       tCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.T.GGG.Ggtt                                                                                             <  1:2520623/101‑1 (MQ=255)
        cAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.T.GGG.Ggttt                                                                                            <  1:3695419/101‑2 (MQ=255)
        cAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.T.GGG.Ggttt                                                                                            <  2:1045617/101‑2 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.G.ggg.ggggtg                                                                                           >  2:187711/1‑96 (MQ=255)
          ggTCGGCGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTca                                                                                           <  1:3478253/101‑1 (MQ=255)
          ggTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.T.ggg.gggttca                                                                                          >  1:1344206/1‑95 (MQ=255)
           gTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.G.ggg.gggttcac                                                                                         >  1:3291226/1‑94 (MQ=255)
           gTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA..GGG.G.ggg.gggttcac                                                                                         >  2:4617122/1‑94 (MQ=255)
               aCGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTcaccccc                                                                                      <  2:3712480/101‑1 (MQ=255)
                 gtgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTcacccccac                                                                                    <  2:4292672/101‑1 (MQ=255)
                 gtgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTcacccccac                                                                                    <  2:4011210/101‑1 (MQ=255)
                   gtcgtcGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTcacccccacac                                                                                  <  2:1619982/101‑1 (MQ=255)
                   gtcgtcGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GGTTcccccccccac                                                                                  >  1:1848330/1‑101 (MQ=255)
                     cgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCGGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTcacccccacaccc                                                                                <  2:5769613/101‑1 (MQ=255)
                      gtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTcacccccacacccc                                                                               <  2:4166680/101‑1 (MQ=255)
                      gtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGCCGGCGCAGGGGGAG.GGG.G.GGG.GTTTcacccccccccccc                                                                               >  1:3537145/1‑101 (MQ=255)
                       tggggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTcacccccacaccccc                                                                              <  1:1412207/99‑1 (MQ=255)
                       tcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GGTTcacccccaccccccc                                                                              >  2:2456937/1‑101 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG.GGG.G.GGG.GGTTcacccccccccccccc                                                                             >  1:5742806/1‑100 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG.GGG.G.GGG.GGTTcacccccccccccccc                                                                             >  1:5629945/1‑100 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG.GGG.G.GGG.GGTTcacccccacaccccca                                                                             >  1:5239221/1‑101 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTcacccccacaccccca                                                                             <  1:4485831/101‑1 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GGTTcacccccacacccccc                                                                             >  2:2915418/1‑100 (MQ=255)
                         gggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAg                                                                            <  1:1066466/101‑1 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG.GGG.G.GGG.GGTTCACCCCCCCACCCCCCGc                                                                           >  1:2252552/1‑101 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGc                                                                           <  1:2809490/101‑1 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGc                                                                           <  2:2291094/101‑1 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGc                                                                           <  2:5480581/101‑1 (MQ=255)
                             gTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTa                                                                       <  1:3951171/101‑1 (MQ=255)
                               ggAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTAtt                                                                     <  2:1894583/101‑1 (MQ=255)
                                gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG.GGG.G.GGG.GGTTCACCCCCACACCCCCCGC.GGAttt                                                                    >  1:5374608/1‑101 (MQ=255)
                                gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTAttt                                                                    <  2:517160/101‑1 (MQ=255)
                                  aTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTg                                                                  <  2:5055364/101‑1 (MQ=255)
                                  aTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTg                                                                  <  1:3069448/101‑1 (MQ=255)
                                   tCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG.GGG.G.GGG.GGGTTACCCCCCCACCCCCCCC.GTGtttttg                                                                 >  2:1191881/1‑99 (MQ=255)
                                    cGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GGTTCACCCCCACACCCCCCGC.GTATTTTGGc                                                                >  2:3552592/1‑101 (MQ=255)
                                     ggCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGcc                                                               <  1:5489842/101‑1 (MQ=255)
                                      gcgcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG.GGG.G.GGG.GGTTCACCCCCACACCCCCAGC.GTATCTTGGCCa                                                              >  2:5791513/1‑101 (MQ=255)
                                      gcgcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCa                                                              <  1:4254771/101‑1 (MQ=255)
                                      gcgcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCa                                                              <  1:1142757/101‑1 (MQ=255)
                                        gcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.G.GGG.GTTTCCCCCCCCCCCCCCCGCC.GAATTTTGGCCaa                                                             >  2:1305456/1‑101 (MQ=255)
                                        gcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCaag                                                            <  2:5282694/101‑1 (MQ=255)
                                          ccGGGGTCAAGGAGGCCGCACGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCaagaa                                                          >  2:335233/1‑101 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GGTTCACCCCCACACCCCCAGC.GTATTTTGGCCaagaag                                                         >  2:802605/1‑101 (MQ=255)
                                            ggggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAagaaga                                                        <  1:1679415/101‑1 (MQ=255)
                                            ggggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAagaaga                                                        <  1:416460/101‑1 (MQ=255)
                                             gggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAgaagaa                                                       <  2:5017728/101‑1 (MQ=255)
                                               gTCAAGGGGGCCGCCGGGGTTGCCGCGTTTGTCGCGGCGGCGCAGGGGTA..GGG.T.GGGTTTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAAc                                                     <  1:83287/101‑1 (MQ=255)
                                               gTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAAc                                                     <  1:4748557/101‑1 (MQ=255)
                                               gTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAAc                                                     <  2:212283/101‑1 (MQ=255)
                                               gTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAAc                                                     <  2:4866594/101‑1 (MQ=255)
                                                tCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTGAGCCCCACACCCCCCGC.GTATTTTGGTCAAGAAGCAACa                                                    >  2:558701/1‑101 (MQ=255)
                                                tCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACa                                                    <  1:1500024/101‑1 (MQ=255)
                                                 cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCAGCCCCACACCCCCAGC.GTATTTTGGCCCAGAAGAAACAg                                                   >  2:3447845/1‑101 (MQ=255)
                                                 cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAg                                                   <  2:4469962/101‑1 (MQ=255)
                                                  aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG.GGG.G.GGG.GGTTTACCCCCACCCCCCCCCCAGCTTTTTGGCCAAaaagaacaag                                                   >  1:212533/1‑97 (MQ=255)
                                                  aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAgg                                                  <  2:3914554/101‑1 (MQ=255)
                                                   aggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGa                                                 <  2:2359529/101‑1 (MQ=255)
                                                   aggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGa                                                 <  1:1367501/101‑1 (MQ=255)
                                                   aggaggCAGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGa                                                 >  2:3800966/1‑101 (MQ=255)
                                                    ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGaa                                                <  2:2550825/101‑1 (MQ=255)
                                                    ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGaa                                                <  1:1549160/101‑1 (MQ=255)
                                                      aggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.G.GGG.GTTTCCCCCCCCCCCCCCCGCC.GAATTTTGGCCAGAAGGAACCAGGaat                                               >  2:1360240/1‑100 (MQ=255)
                                                       ggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCCCCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.Ctt                                            <  1:4154040/101‑1 (MQ=255)
                                                       ggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGACACAGGAA.Ctt                                            <  1:5000551/101‑1 (MQ=255)
                                                         ccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCGACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTa                                          <  2:2955415/101‑1 (MQ=255)
                                                         ccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTa                                          <  2:3676479/101‑1 (MQ=255)
                                                            cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTAccc                                       <  2:4636530/101‑1 (MQ=255)
                                                            cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTAccc                                       >  2:2876107/1‑101 (MQ=255)
                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCg                                     <  2:5380720/101‑1 (MQ=255)
                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCg                                     <  1:124620/101‑1 (MQ=255)
                                                               ggATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG.GGG.G.GGG.GGTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGa                                    >  1:3236817/1‑101 (MQ=255)
                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.g                                  <  2:5742511/101‑1 (MQ=255)
                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GGTTCACCCCCACACCCCCAGC.GTATTTTGGCCCAGAAGAAACAGGAA.CTTTACCCCGA.g                                  >  1:632561/1‑101 (MQ=255)
                                                                 aTTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.gg                                 <  1:4631467/101‑1 (MQ=255)
                                                                  ttGCCGCCTTTGTTGCGGTGTCGCACGGGTA..GGGCT.GGG.GCTTCACGCCCACACCCCCAGC.GTCTTTTGGGCAAGAAGAAATAGGCA.TTTTACCCCGC.ggg                                >  2:491150/1‑101 (MQ=255)
                                                                   tGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.gggg                               <  2:5629653/101‑1 (MQ=255)
                                                                     ccgccTTTGTCGCGCCGGGGCAGGGGTA..GGG.TGGGG.GCTTCACCCCAACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.cggggg                             <  2:3857034/101‑1 (MQ=255)
                                                                             gTCGCGGCGGCGCAGGGGTA..GGG.TGGGG.GGTTCCCCCCCCCCCCCCCCGC.GTATTTTTGGCCACAAGAAAAAGGAACCTTTCCCCCGC.gggggggggcggc                      >  2:3105378/1‑96 (MQ=255)
                                                                             gTCGCGGCGGAGGAGGGGTA..GGG.TGGGG.GTTTCCCCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCt                     <  1:3453160/101‑1 (MQ=255)
                                                                               cgcgGCGGCGCAGGGGTAG.GGG.G.GGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTAt                   >  2:5214029/1‑101 (MQ=255)
                                                                               cgcgGCGGCGCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTAt                   <  2:2467659/101‑1 (MQ=255)
                                                                                gcggcggcgCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATc                  <  1:2446301/101‑1 (MQ=255)
                                                                                  ggcggcgCAGGGGTA..GGG.TGGGG.GGTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATctg                >  1:3094022/1‑101 (MQ=255)
                                                                                   gcggcgCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATctgc               <  1:297402/101‑1 (MQ=255)
                                                                                   gcggcgCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATctgc               <  2:3514531/101‑1 (MQ=255)
                                                                                    cggcgCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGAGGGGGGGGGCGCGCTATctgc               >  1:1753147/1‑101 (MQ=255)
                                                                                     ggcgCAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATctgctg             <  2:4927434/101‑1 (MQ=255)
                                                                                       cgcAGGGGTA..GGG.TGGGG.GTTTCCCCCCCCCCCCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATCTGCTGtt           <  1:3815484/101‑1 (MQ=255)
                                                                                         cAGGGGTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTgg         <  1:775305/101‑1 (MQ=255)
                                                                                          aGGGGTA..GGG.TGGGG.GGTTTACCCCCACACCCCCAGC.GTATTTTTGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCGATCTGCTGTTggg        >  2:2981449/1‑100 (MQ=255)
                                                                                            gggTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGGGCGCCATCTGCTGTTGgcgc      >  2:537069/1‑101 (MQ=255)
                                                                                             ggTA..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGGcgcg     >  2:454629/1‑101 (MQ=255)
                                                                                                a..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCGAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  <  2:4109987/101‑1 (MQ=255)
                                                                                                a..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  >  2:2797896/1‑101 (MQ=255)
                                                                                                a..GGG.TGGGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  >  1:3439220/1‑101 (MQ=255)
                                                                                                        |                                                                                                   
GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA..GGG.T.GGG.GTTTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAA.CTTTACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGA  >  NC_014034/1200694‑1200889

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG