BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,761	+A	10.1%	intergenic (+573/‑358)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,761	1	.→A	10.1%	132.2 ‑0.2 ‑0.4	326	intergenic (+573/‑358)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (14/19): ref base (130/163): total (144/182)
Fisher's exact test strand distribution p-value = 8.56e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.72e-01

Only 100 of 367 total aligned reads displayed.


AAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395661‑1395861


aaaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g                                                                                                       <  1:568993/101‑1 (MQ=255)


aaaCATCGGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g                                                                                                       <  2:4198579/101‑1 (MQ=255)


 aaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g.t                                                                                                     >  2:4846339/1‑101 (MQ=255)


 aaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g.t                                                                                                     >  1:4311345/1‑101 (MQ=255)


 aaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g.t                                                                                                     >  1:3558472/1‑101 (MQ=255)


 aaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g.t                                                                                                     >  1:3056047/1‑101 (MQ=255)


 aaCATCTGCATGTAGGCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g.t                                                                                                     <  1:1067295/101‑1 (MQ=255)


  aCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g.tc                                                                                                    >  2:4599958/1‑101 (MQ=255)


   cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g.tcg                                                                                                   >  2:2760594/1‑101 (MQ=255)


   cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g.tcg                                                                                                   >  2:5626044/1‑101 (MQ=255)


    aTCTGCAGGTAGTCGGCGGGCAGTCCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.g.tcgt                                                                                                  <  2:4097254/101‑1 (MQ=255)


        gCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGcc                                                                                              >  1:3102790/1‑100 (MQ=255)


         cATGTGGTCGGCGCGCAGTTCGAGCCGCAGTCGGTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTc                                                                                             <  1:4976500/101‑1 (MQ=255)


         cATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTc                                                                                             >  2:3288296/1‑101 (MQ=255)


           tGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccc                                                                                           <  2:4073200/101‑1 (MQ=255)


              aggcggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccccc                                                                                        <  1:3837876/98‑1 (MQ=255)


                tgggcggGCATTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcgc                                                                                      <  2:5201928/99‑1 (MQ=255)


                  ggcggCCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgg                                                                                    <  2:5043636/101‑1 (MQ=255)


                        cAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ACCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCgg                                                                              >  2:1296231/1‑100 (MQ=255)


                          tgtgcaGCCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTgg                                                                            <  2:3618546/96‑1 (MQ=255)


                          gtcggAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTgg                                                                            <  2:5332759/97‑1 (MQ=255)


                          gTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTgg                                                                            >  1:196773/1‑101 (MQ=255)


                           ttCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                           <  1:1465698/101‑1 (MQ=255)


                           ttCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                           <  2:5761060/101‑1 (MQ=255)


                             caagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCAAAACTCGGCAAGG.ACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTgg                                                                            >  1:3652328/8‑101 (MQ=2)


                             cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         >  1:3752193/1‑101 (MQ=255)


                              gAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        >  2:4495541/1‑101 (MQ=255)


                                gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        <  1:1884532/95‑1 (MQ=0)


                                gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                      <  1:491230/101‑1 (MQ=255)


                                gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                      <  2:4897272/101‑1 (MQ=255)


                                 attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGG.ACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        >  2:3052593/4‑101 (MQ=2)


                                 attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGG.ACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        >  1:1238478/4‑101 (MQ=2)


                                  gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCCaa                                                                      >  1:773573/5‑101 (MQ=0)


                                   gCAGTCGCTCCCACCGCGGGGGCGTGGATCGAAACT.GGTATGG.CTCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                   >  1:3133615/1‑101 (MQ=255)


                                    tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    <  1:2735715/99‑1 (MQ=2)


                                    tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    <  2:4029717/99‑1 (MQ=2)


                                    cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGG.ACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     <  1:875239/101‑1 (MQ=2)


                                     ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                   >  2:5300578/2‑101 (MQ=2)


                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGG.ACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    >  1:2615394/1‑101 (MQ=2)


                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGG.ACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    >  1:2374453/1‑101 (MQ=2)


                                     aGTCGCCCCCCGCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                                                 <  2:3452869/101‑1 (MQ=255)


                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.ATGGCCAGAT..CCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                                  <  2:696738/101‑1 (MQ=2)


                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                                  <  2:5368573/101‑1 (MQ=2)


                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                                >  1:2485232/1‑101 (MQ=255)


                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                                <  1:5223186/101‑1 (MQ=255)


                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCta                                                                 >  2:789097/1‑100 (MQ=2)


                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                                >  1:1687567/1‑99 (MQ=2)


                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcgg                                                              <  2:1564299/101‑1 (MQ=255)


                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcgg                                                              >  2:3314413/1‑101 (MQ=255)


                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                               >  1:1576866/1‑98 (MQ=2)


                                          cTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatga                                                              <  1:5066891/101‑5 (MQ=2)


                                           tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                             <  1:973622/101‑6 (MQ=2)


                                           tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.G.ACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                             >  1:4546118/1‑96 (MQ=2)


                                           tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcggcgg                                                           >  2:3778981/1‑100 (MQ=255)


                                            ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGG.ACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                             >  1:4742742/1‑94 (MQ=0)


                                             cccccGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGGGGCGTgc                                                         >  2:2634501/1‑100 (MQ=255)


                                             cccccGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGa                                                         <  2:1458257/101‑1 (MQ=255)


                                                ccGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGtt                                                      >  2:882449/1‑101 (MQ=255)


                                                    gggggCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTTGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                                                  >  2:238112/1‑101 (MQ=255)


                                                    gggggCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                                                  >  2:1156296/1‑101 (MQ=255)


                                                      gggCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGt                                                <  2:1056214/101‑1 (MQ=255)


                                                       ggCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTg                                               <  1:2922227/101‑1 (MQ=255)


                                                        gCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGa                                              >  2:3422218/1‑101 (MQ=255)


                                                          gTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                                            >  2:5705846/1‑101 (MQ=255)


                                                           tGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                                           >  1:2220312/1‑101 (MQ=255)


                                                           tGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                                           >  2:3183469/1‑101 (MQ=255)


                                                           tGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                                           >  1:1157957/1‑101 (MQ=255)


                                                            ggATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGa                                          >  2:2936542/1‑101 (MQ=255)


                                                             gATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGAc                                         <  1:5361798/101‑1 (MQ=255)


                                                             gATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGAc                                         >  2:1972324/1‑101 (MQ=255)


                                                               tCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                                       <  1:3194997/101‑1 (MQ=255)


                                                                   aaCT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTg                                   >  1:3271256/1‑101 (MQ=255)


                                                                    aCT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTgg                                  <  2:2460646/101‑1 (MQ=255)


                                                                     cT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa                                 <  1:560281/101‑1 (MQ=255)


                                                                     cT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa                                 >  2:3091211/1‑101 (MQ=255)


                                                                     cT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa                                 >  1:2095772/1‑101 (MQ=255)


                                                                      t.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAc                                >  1:4858102/1‑101 (MQ=255)


                                                                        ggTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACg                               >  1:172967/1‑101 (MQ=255)


                                                                          tATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc                             >  2:2291426/1‑101 (MQ=255)


                                                                          tATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc                             <  1:4656379/101‑1 (MQ=255)


                                                                           aTGG.ATCTG..GCTCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCg                            <  2:4717644/101‑1 (MQ=255)


                                                                            tGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGc                           <  2:2100366/101‑1 (MQ=255)


                                                                             gg.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGcc                          >  1:3998675/1‑100 (MQ=255)


                                                                                  cTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccc                      <  2:3701831/101‑1 (MQ=255)


                                                                                   tG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTccccc                     >  1:5510495/1‑101 (MQ=255)


                                                                                    g..gCGCAAGCGG..AAAGTCT.G.TCGTCGCGCCCCCCGCGGGGGCGTGGAGCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCGcccccc                    <  1:3901367/101‑1 (MQ=38)


                                                                                        cgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCcgc                  <  1:4109664/101‑1 (MQ=255)


                                                                                        cgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCcgc                  >  2:3810500/1‑101 (MQ=255)


                                                                                         gcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCcccg                 >  1:3373228/1‑101 (MQ=255)


                                                                                           aaaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCggg               >  1:4420985/1‑101 (MQ=255)


                                                                                            aaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTccccccagatcg              >  2:657197/1‑95 (MQ=255)


                                                                                              cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGc            >  2:315827/1‑101 (MQ=255)


                                                                                                   aaaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTg         >  1:1023535/1‑101 (MQ=255)


                                                                                                   aaaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTg         <  1:4804818/101‑1 (MQ=255)


                                                                                                    aaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTgg        <  1:5762172/101‑1 (MQ=255)


                                                                                                      gTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGAt      <  1:664766/101‑1 (MQ=255)


                                                                                                      gTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGGGGAt      >  2:5530636/1‑101 (MQ=255)


                                                                                                       tCT.G.TCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:4972843/101‑1 (MQ=255)


                                                                                                        cT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGGTGCCAGTGATAGACGCCGTGGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  2:1814693/1‑101 (MQ=255)


                                                                                                           g.tcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  1:1296314/101‑1 (MQ=255)


AAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGG.ATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395661‑1395861

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG