BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,935	+T	8.1%	coding (817/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,935	1	.→T	8.1%	147.8 ‑0.3 ‑0.5	471	coding (817/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (19/19): ref base (231/202): total (250/221)
Fisher's exact test strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.45e-01

Only 100 of 493 total aligned reads displayed.


GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGT  >  NC_014034/1396835‑1397035


  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.Gc                                                                                                               <  2:5352753/101‑1 (MQ=255)


    gCTCCCCCCGCGGGGGCGTGGTTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCt                                                                                                             <  1:2297570/101‑1 (MQ=255)


     cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                           <  2:3331613/101‑1 (MQ=255)


     cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                           >  1:1763078/1‑101 (MQ=255)


       ccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcg                                                                                                         <  1:3562995/101‑1 (MQ=255)


       ccccccGCGGGGGCGTGGATCCAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcg                                                                                                         <  2:5738093/101‑1 (MQ=255)


        cccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcgc                                                                                                        >  1:5195142/1‑101 (MQ=255)


         ccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.Gcgcg                                                                                                       <  1:1957111/101‑1 (MQ=255)


               gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                               >  2:2581933/1‑101 (MQ=255)


                 gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                            <  2:1908155/101‑1 (MQ=255)


                 gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                            <  2:4804509/101‑1 (MQ=255)


                 gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                            <  1:625477/101‑1 (MQ=255)


                  ggccgggATCGAAACCGGTCCACGGAAAGACCCCGCCGGCCCCCTCCGGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                           <  2:4949039/96‑1 (MQ=21)


                  ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                           <  1:4233395/101‑1 (MQ=255)


                   ggggggATCGAACCCGGTCCAGAGATAGACCGCGCGGGCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCgg                                                                                          <  2:4423979/97‑1 (MQ=255)


                     gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.Ct                                                                                       <  2:2401708/101‑1 (MQ=255)


                      tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.Ct.t                                                                                     <  2:449878/101‑1 (MQ=255)


                       ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.Tc                                                                                    >  1:1639557/1‑101 (MQ=255)


                         aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.Tc.cc                                                                                 >  1:2231839/1‑100 (MQ=255)


                           cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGc.c                                                                              >  2:4354243/1‑101 (MQ=255)


                           cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGc.c                                                                              >  1:5010490/1‑101 (MQ=255)


                             aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTg                                                                            <  2:2011086/101‑1 (MQ=255)


                             aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTg                                                                            >  2:5154856/1‑101 (MQ=255)


                             aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTg                                                                            >  2:4284285/1‑101 (MQ=255)


                                  ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTg.g                                                                    >  1:3615004/1‑101 (MQ=255)


                                   gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.Gt                                                                   <  1:3936871/101‑1 (MQ=255)


                                   gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.Gt                                                                   <  2:4696031/101‑1 (MQ=255)


                                    tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTc                                                                  <  1:1122374/101‑1 (MQ=255)


                                      cAGAGATAGACCGCGCGGCCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGc                                                                <  1:4814253/101‑1 (MQ=255)


                                        gagaTAGACCGCGCGGCCCTCCTCAGACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTc                                                              <  1:3080646/101‑1 (MQ=255)


                                        gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTc                                                              >  2:4868497/1‑101 (MQ=255)


                                        gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTc                                                              <  1:4563218/101‑1 (MQ=255)


                                         agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTcc                                                             <  2:735996/101‑1 (MQ=255)


                                           aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTcccc                                                           >  1:4650585/1‑101 (MQ=255)


                                           aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTcccc                                                           <  1:2961852/101‑1 (MQ=255)


                                             aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                         <  2:1070310/101‑1 (MQ=255)


                                              gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCcg                                                        <  1:2703535/101‑1 (MQ=255)


                                                ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GGCGCTCCCCCcgcg                                                      >  1:1269027/1‑101 (MQ=255)


                                                 cgatcgGCCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                     <  2:1187272/97‑1 (MQ=255)


                                                  gcgcgAACCTCCTCAGACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                    <  2:154493/101‑1 (MQ=255)


                                                   cgcgGCCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCgggg                                                   <  1:1016808/101‑1 (MQ=255)


                                                      cgACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                <  1:2075032/100‑1 (MQ=255)


                                                        gcctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                              <  2:37123/100‑1 (MQ=255)


                                                        aCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                              <  1:1369689/101‑1 (MQ=255)


                                                         cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                             <  2:620516/101‑1 (MQ=255)


                                                           ccctcAGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:213819/100‑1 (MQ=255)


                                                            tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  1:3697366/9‑101 (MQ=2)


                                                             ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  1:5312254/94‑1 (MQ=2)


                                                             ggccccagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCCTATG.CGC.TTGA.CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  1:3268649/94‑1 (MQ=2)


                                                             ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  2:4610396/1‑101 (MQ=255)


                                                             ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GGCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:871400/1‑101 (MQ=255)


                                                              ccAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  2:1721510/100‑1 (MQ=255)


                                                               cccccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  2:500830/97‑1 (MQ=2)


                                                               acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  2:946426/6‑101 (MQ=9)


                                                                aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      >  2:683994/1‑101 (MQ=255)


                                                                 actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      >  2:1388078/4‑101 (MQ=9)


                                                                  ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     >  1:1803395/3‑101 (MQ=9)


                                                                  cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                    <  2:3559995/99‑2 (MQ=2)


                                                                  ccGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  2:3803703/100‑1 (MQ=2)


                                                                  aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                    <  1:5548107/101‑1 (MQ=255)


                                                                   tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.ACGG.ATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                    >  1:4897224/2‑100 (MQ=9)


                                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     <  2:3060844/101‑1 (MQ=2)


                                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                   <  2:2254809/101‑3 (MQ=9)


                                                                    gTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                  <  2:3895656/101‑1 (MQ=255)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                    >  2:5553029/1‑101 (MQ=2)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccg                                  <  1:1550236/101‑4 (MQ=2)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccc                                  >  1:2452479/1‑98 (MQ=2)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                 <  2:2763548/101‑1 (MQ=255)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                 >  1:4289973/1‑101 (MQ=255)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG.AGGG.CG.TCTGGC.ACA..GGAC.GTCTCTCCCCCCGCGGGGGCGTGGGTCGaaagaat                                  >  2:5623443/1‑97 (MQ=2)


                                                                     tCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                 <  2:3614776/101‑1 (MQ=255)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGGGGATGGaaaaac                                   >  2:2966229/1‑98 (MQ=2)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                               <  1:4399238/101‑1 (MQ=255)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                               <  1:2846534/101‑1 (MQ=255)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                               <  1:4114767/101‑7 (MQ=2)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                               >  2:2338114/1‑95 (MQ=2)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCa                                 >  1:5382939/1‑101 (MQ=2)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                               >  2:1816358/1‑95 (MQ=2)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCt                              >  1:3780178/1‑101 (MQ=255)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                              <  2:2829983/101‑8 (MQ=2)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                >  2:3445870/1‑100 (MQ=2)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCG.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                             <  1:1685322/101‑1 (MQ=255)


                                                                          ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                             <  2:3240912/101‑9 (MQ=9)


                                                                           cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                              <  2:5061901/101‑4 (MQ=2)


                                                                           cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                           <  2:4275839/101‑1 (MQ=255)


                                                                           cccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgggg                           <  1:2181086/101‑11 (MQ=2)


                                                                           cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                            <  2:311322/101‑10 (MQ=9)


                                                                            ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                             <  1:807733/101‑5 (MQ=2)


                                                                            ccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                           <  2:785092/101‑11 (MQ=2)


                                                                             tccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTT..CGTG.GTCGCCTCCCCCCAGCGGGCGGCGATCGTAACCTCCGCttggcc                         >  1:5687879/2‑96 (MQ=255)


                                                                               cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                       >  2:1609794/1‑101 (MQ=255)


                                                                                    gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                  >  2:3984824/1‑101 (MQ=255)


                                                                                    gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                  <  1:4049261/101‑1 (MQ=255)


                                                                                      gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.AT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                >  2:5228674/1‑101 (MQ=255)


                                                                                        gTGGATCGAAACA.GCCG.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg              <  1:1189524/101‑1 (MQ=255)


                                                                                         tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg             >  1:184949/1‑101 (MQ=255)


                                                                                           gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc           >  2:1293455/1‑101 (MQ=255)


                                                                                           gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc           <  2:871324/101‑1 (MQ=255)


                                                                                           gATCAAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc           <  2:4188171/101‑1 (MQ=255)


                                                                                                aaaCA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt      >  1:1460664/1‑101 (MQ=255)


GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGT  >  NC_014034/1396835‑1397035

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG