Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,009 +A 15.5% coding (891/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,0091.→A15.5% 220.1 ‑0.8 ‑0.1341coding (891/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (24/28):  ref base (152/136):  total (176/165)
Fisher's exact test strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.36e-01

Only 100 of 373 total aligned reads displayed.

CCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCC  >  NC_014034/1396909‑1397109
                                                                                                     |                                                                                                         
ccccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                                                                            >  1:4191078/1‑101 (MQ=255)
 cccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgc                                                                                                           >  2:2087085/1‑100 (MQ=255)
 cccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg.t                                                                                                          <  1:1579473/101‑1 (MQ=255)
       cgGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.GCGTTgg                                                                                                    >  2:4873527/1‑101 (MQ=255)
        gggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGt                                                                                                   >  2:579705/1‑101 (MQ=255)
          gggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCa                                                                                                 >  2:3984824/1‑101 (MQ=255)
          gggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCa                                                                                                 <  1:4049261/101‑1 (MQ=255)
           ggCGGGGATCGAAACAGCCGGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAg                                                                                                <  2:4279032/101‑1 (MQ=255)
            gCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGc                                                                                               >  2:10536/1‑101 (MQ=255)
            gCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGATTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGc                                                                                               >  2:5228674/1‑101 (MQ=255)
             cGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGcc                                                                                              >  2:1250221/1‑101 (MQ=255)
             cGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGcc                                                                                              <  1:3035518/101‑1 (MQ=255)
             cGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGcc                                                                                              >  1:1735189/1‑101 (MQ=255)
               tGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCgg                                                                                            >  1:655194/1‑101 (MQ=255)
                ggATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.a                                                                                          <  2:1862340/101‑1 (MQ=255)
                 gATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.Ac                                                                                         >  2:1293455/1‑101 (MQ=255)
                    cGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GAt                                                                                     >  1:2833236/1‑101 (MQ=255)
                    cGAAACAGCCTGCGCGGCCGCACCGGCTTCCGCCTGCGTGGCCGCTCCCCCCGCGGGGGCGAGGGTCGAAACATCAGCTCG.TCCTCGGACAGccctaccag                                                                                       >  2:289867/1‑94 (MQ=37)
                     gAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATg                                                                                    >  2:456703/1‑101 (MQ=255)
                     gAAACAGCCTGCGCGGCCGCATCGGCTTCCGACTGCGTGGTTGCCTCCCCCGCGGGGGCGTGAATCGAAACATCAGCTCG.TCGTTTGGCAGCCGG.AC.GATg                                                                                    >  2:3817537/1‑101 (MQ=255)
                      aaaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGt                                                                                   >  2:3612103/1‑101 (MQ=255)
                       aaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTc                                                                                  <  2:4751067/101‑1 (MQ=255)
                          aGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...Gt                                                                            <  2:1605084/101‑1 (MQ=255)
                           gCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTc                                                                           <  1:1370919/101‑1 (MQ=255)
                              tGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCt                                                                        <  1:4454814/101‑1 (MQ=255)
                               gcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTc                                                                       <  2:216078/101‑1 (MQ=255)
                                 gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccc                                                                     <  1:5362718/101‑1 (MQ=255)
                                 gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccc                                                                     >  2:2329306/1‑101 (MQ=255)
                                 gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccc                                                                     <  2:2824837/101‑1 (MQ=255)
                                   ggCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccccc                                                                   <  1:4284545/101‑1 (MQ=255)
                                   ggCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCC.TCGTTGGTCAGCCCG.AC.GATGTCC...GCCACTccccc                                                                   >  1:4542785/1‑101 (MQ=38)
                                   ggCCGCATCGGCTTCCGCCTGCGTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccccc                                                                   <  1:4135302/101‑1 (MQ=255)
                                       gCATCCGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCcgcg                                                               <  2:4333246/101‑1 (MQ=255)
                                        cATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCgg                                                              >  1:1832356/1‑101 (MQ=255)
                                         aTCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCggg                                                             >  1:5568686/1‑101 (MQ=255)
                                          tCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCgggg                                                            <  2:4190828/101‑1 (MQ=255)
                                             gCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCg                                                         >  1:1860803/1‑101 (MQ=255)
                                              cTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCgg                                                        >  2:605655/1‑100 (MQ=255)
                                               ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                       >  1:1041593/1‑101 (MQ=255)
                                               ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                       >  1:1897822/1‑101 (MQ=255)
                                               ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                       >  2:4410464/1‑101 (MQ=255)
                                               ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                       >  2:4502493/1‑101 (MQ=255)
                                               ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                       <  1:342192/101‑1 (MQ=255)
                                                tCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                      <  2:296242/101‑1 (MQ=255)
                                                 ggatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTg                                                       <  1:2293976/91‑1 (MQ=2)
                                                  gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                      <  2:3335149/92‑1 (MQ=2)
                                                  cGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:3767950/101‑1 (MQ=255)
                                                   gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:1250304/101‑1 (MQ=255)
                                                   gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   >  1:5736583/1‑101 (MQ=255)
                                                    ccagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    >  2:832349/9‑101 (MQ=2)
                                                     cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:5105474/95‑1 (MQ=2)
                                                      tGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                >  1:179524/1‑101 (MQ=255)
                                                      tGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                >  1:695705/1‑101 (MQ=255)
                                                      ccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  >  1:5223523/6‑101 (MQ=2)
                                                       cgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                 <  1:1132375/97‑1 (MQ=2)
                                                        cggcgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  1:1957789/97‑1 (MQ=2)
                                                        cGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              <  1:50238/101‑1 (MQ=255)
                                                         gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                             <  2:1639463/101‑1 (MQ=255)
                                                         gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                             <  2:5483654/101‑1 (MQ=255)
                                                         gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                             <  1:2508142/101‑1 (MQ=255)
                                                         ccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCC.CATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  1:5468170/99‑1 (MQ=2)
                                                          tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACtc                                            >  1:2672121/1‑100 (MQ=255)
                                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                             <  1:1452261/101‑2 (MQ=2)
                                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGc                                           >  1:2042340/1‑101 (MQ=2)
                                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCg                                         >  1:3329941/1‑101 (MQ=255)
                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaact                                          >  1:2381289/1‑97 (MQ=2)
                                                                cTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCt                                      >  1:382484/1‑101 (MQ=255)
                                                                 tCCCCCCGCGGGGGCGTTGATCGAAACATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                       <  2:5146270/101‑5 (MQ=2)
                                                                 cccccccGCGGTGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGG.TCACATCCCC...GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                       <  2:5703996/100‑5 (MQ=2)
                                                                 cccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                     <  2:4150303/100‑1 (MQ=255)
                                                                  ccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                    <  2:1996031/101‑1 (MQ=255)
                                                                   cccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagat                                     >  1:3202015/1‑95 (MQ=2)
                                                                   cccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGa                                   <  2:3456013/101‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                 <  2:5031921/101‑1 (MQ=255)
                                                                      ccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTg                                <  1:1320834/101‑1 (MQ=255)
                                                                       cgcgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGa                               >  2:5307689/1‑101 (MQ=255)
                                                                         cgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATg                             >  1:2946031/1‑101 (MQ=255)
                                                                           ggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                           <  1:1609888/101‑1 (MQ=255)
                                                                             ggCGTGGATCGAAACATCACCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgc                         >  1:4358509/1‑101 (MQ=255)
                                                                              gCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                        <  2:268705/101‑1 (MQ=255)
                                                                              gCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                        <  2:1384779/101‑1 (MQ=255)
                                                                              gCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                        >  1:1000163/1‑101 (MQ=255)
                                                                               cGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcgc                       >  1:3568282/1‑101 (MQ=255)
                                                                                gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                      >  2:2233359/1‑101 (MQ=255)
                                                                                gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                      <  2:1776167/101‑1 (MQ=255)
                                                                                 tGGATCGAAACATCAGCTCG.TCGCTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACTGCCGTCTTGAGTGATGGGGcgcgg                     >  2:1678559/1‑100 (MQ=255)
                                                                                  ggATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                    >  2:69822/1‑101 (MQ=255)
                                                                                   gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                   <  2:4978754/101‑1 (MQ=255)
                                                                                    aTCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcgggggcc                  >  1:4661654/1‑95 (MQ=255)
                                                                                       gAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt               <  1:4902285/101‑1 (MQ=255)
                                                                                       gAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt               <  1:21809/101‑1 (MQ=255)
                                                                                        aaaCATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg              <  2:2456357/101‑1 (MQ=255)
                                                                                           cATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg           >  2:3262754/1‑101 (MQ=255)
                                                                                            aTCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgt          >  2:1140062/1‑101 (MQ=255)
                                                                                             tCAGCTCG.TCGTTTGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgtc         >  2:8891/1‑101 (MQ=255)
                                                                                              cAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATctcgtcc        >  2:5620404/1‑100 (MQ=255)
                                                                                               aGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGc       <  1:3475047/101‑1 (MQ=255)
                                                                                               aGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGc       >  2:5080520/1‑101 (MQ=255)
                                                                                                 cTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGAGCGAGCACATGTCGTCGCTc     >  2:375459/1‑101 (MQ=255)
                                                                                                   cg.tcgtTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccc   >  1:4791343/1‑101 (MQ=255)
                                                                                                     |                                                                                                         
CCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCC  >  NC_014034/1396909‑1397109

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG