Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,732,866 +G 90.2% intergenic (‑290/‑113) RCAP_rcc01601 ← / → tgt FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,732,8663.→G90.2% 7.3 0.0 ‑0.641intergenic (‑290/‑113)RCAP_rcc01601/tgtFeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (7/28):  ref base (1/3):  total (8/33)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.45e-01

CTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.C....GT...C...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGGCG  >  NC_014034/1732766‑1732949
                                                                                                         |                                                                                                
cTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGcccc...c.c                                                                                                   <  2:3586581/101‑1 (MQ=255)
cTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAGcgcc...c.c                                                                                                   >  1:689170/1‑101 (MQ=255)
 ggCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.C....g                                                                                              <  1:2636303/100‑1 (MQ=255)
   cAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.C....gcg                                                                                            <  2:2782920/101‑3 (MQ=255)
   cAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGGGcccc...c.cccg                                                                                                >  2:3538509/1‑98 (MQ=255)
    aGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAGgccc...c.cccgg                                                                                               >  2:4642426/1‑97 (MQ=255)
    aGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAGcccc...c.cccgg                                                                                               >  1:5503465/1‑97 (MQ=255)
     gCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAGgccc...c.ccccgg                                                                                              >  1:5588846/1‑96 (MQ=255)
        gCATGCCGTCCGCGTCAACGGGCAAGGCCCCCTCTTTTCCTAGGCAAAATACCCCCTTTTTTCTTTGCGGGGGGGCGGGGGGTGAAGCCCC...C.C....gcgggtcg                                                                                       <  1:1370606/101‑8 (MQ=25)
        gCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAGgccc...c.cccggggcg                                                                                           >  1:3769685/1‑93 (MQ=255)
         cATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGTGGGGGGTGAAGCCCC...C.C....gcggggcgc                                                                                      >  1:4863383/1‑93 (MQ=255)
                        aaCAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGa                                                                <  1:3845554/101‑1 (MQ=21)
                           aGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGc                                                             <  2:991574/101‑1 (MQ=21)
                                     ccggtttttCCTAGGAAAAATACGCCTTTCTTTCTTTTCGGGGGGGCGGGGGGTGAACCCCC...C.C....GC...GGGTCG....CCTCGGGCTTGCCCGAGGCGAAAGCTTcc                                                   <  2:70871/96‑1 (MQ=17)
                                      ccGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.C....GCGGGT...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTccc                                                  <  1:3610843/101‑1 (MQ=21)
                                         cTTTTCCTAGGCAAAATCCGCCCTTCTTTCTTTGCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTcccccc                                               <  2:3336542/101‑1 (MQ=18)
                                         cTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTcccccc                                               <  2:5781576/101‑1 (MQ=21)
                                            tcccTGGGCAAAATCCGCCCTTCTTTCTTTGCGGGGGGGCGGGGGGTGAACCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTg                                            <  2:3229874/99‑1 (MQ=17)
                                              ccgaGGAAAAATTCGCCCTTTTTGCTTTGGGGGGGGGCGGGGGGGCAACCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGaa                                          <  1:599072/98‑1 (MQ=2)
                                              ccaaGGAAAAATCCGCCTTTTTTTCTTTTGGGGGGGGCGGGGGGGAAACCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGaa                                          <  2:335494/98‑1 (MQ=9)
                                               cTAGGCAAAATCCCCCCTTTTTTCTTTGGGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGaaa                                         <  1:2496240/101‑1 (MQ=14)
                                                 agtcAAAATCCTCCTTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAAc                                       <  2:2537918/98‑1 (MQ=17)
                                                   gCAAAGTACCCCCTTTTTTTTTTTCGGGGGGGGGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCt                                     <  2:5385751/101‑1 (MQ=17)
                                                   gCAAAATACGCCCTTCTTTCTTGTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCt                                     <  2:1652602/101‑1 (MQ=18)
                                                    taaaaTCCGCCCTCGTTTCTTTTGGGGGGGGCGGGAGGTGAAGCCCCTGGC.G....GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCtt                                    <  2:2685915/100‑1 (MQ=12)
                                                    cAAAATCCCCCTTTTTTTTTTTGGGGGGGGGGGGGGGGTGACCCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCtt                                    <  1:2407497/101‑1 (MQ=2)
                                                    cAAAATCCCCCTTTCTTTTTTTGGGGGGGGGGGGGGGGTGAACCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCtt                                    <  2:5068540/101‑1 (MQ=9)
                                                    aaaattccGCCCTTCTTTCTTTGGGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCtt                                    <  2:1954077/94‑1 (MQ=14)
                                                    aaaaactcccccTTTTTTTTTTGGGGGGGGGGGGGGGGGGAACCCCC...C.CCGCGGG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCt                                     <  2:3011927/92‑1 (MQ=2)
                                                       aaTACGCCCTTCTTTCTTTTGGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGcc                                 <  2:1669152/101‑1 (MQ=18)
                                                        aTACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCt                                <  2:868409/101‑1 (MQ=21)
                                                           cGCTCTTCTTTCTTTGCGGGGGGGCGGGGGGGGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTgcg                             <  2:805220/101‑1 (MQ=17)
                                                              ccTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTgcgcgc                          <  2:5079872/101‑1 (MQ=21)
                                                                   tttctttTCGGGGGGGCGGGGGGGGGAGCCCC...C.CCCG.GG...G...GG....CCCCCGGCGTGCCCCAGGCGGAAACGTCCCCCCCCGGAAACCCTGCCCCCGCGCGCCGc                     >  2:4423967/1‑101 (MQ=255)
                                                                      tttttGGGGGGGGGGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                  <  2:5312555/100‑1 (MQ=18)
                                                                       ttttCGGGGGGGGGGGGGGTGAAACCCC...C.CCGCGGG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                  <  2:1349675/101‑1 (MQ=21)
                                                                       ttttCGGGGGGGCGGGGGGGGAAGCCCC...C.C....CC...C...GGGGGCCCCCGGGCTTGCCCCGGGGGAAAGCTTCCCCCCCGGAAACCCTGCCCGCcgcgcgcgccttc                  >  2:2409782/1‑94 (MQ=255)
                                                                       tttgggggggggGGGGGGGTGACCCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTc                 <  1:4642702/96‑1 (MQ=18)
                                                                           ggggggggCGGGGGGTGACCCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCtt             <  2:1427717/100‑1 (MQ=18)
                                                                            gggggggGGGGGGGGGAACCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCttt            <  2:5614901/101‑1 (MQ=18)
                                                                             ggggggCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc           <  2:5463259/101‑1 (MQ=21)
                                                                             ggggggCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc           <  1:3473759/101‑1 (MQ=21)
                                                                             ggggggCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc           <  2:3769442/101‑1 (MQ=21)
                                                                              gggggGGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCg          <  2:4416929/101‑1 (MQ=21)
                                                                               ggggCGGGGGGGGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCgg         <  1:3502339/101‑1 (MQ=21)
                                                                                 ggCGGGGGGTCAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGcc       <  2:2413746/101‑1 (MQ=21)
                                                                                   cGGGGGGTGAAGCCCG...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTTCCTGCGCGCGTCGCTTTCCCTTTTGGCCCg     >  1:4881740/1‑101 (MQ=18)
                                                                                   cGGGGGGTGAAGCCCCT..CGC....GTG..T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg     <  1:3548126/101‑1 (MQ=25)
                                                                                   cGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg     >  2:2458531/1‑101 (MQ=21)
                                                                                    ggggggTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGGCTCGCTTTCCCTTTCGGCCCgg    >  2:296370/1‑101 (MQ=18)
                                                                                    ggggggTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGccccg    >  1:964044/1‑99 (MQ=21)
                                                                                    ggggggTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCgg    >  2:3760503/1‑101 (MQ=21)
                                                                                    ggggggTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCgg    >  2:3026747/1‑101 (MQ=21)
                                                                                     gggggTGAAGCCCC...C.CGCG.GG...G...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGCCGCTTTCCCTTTCTGCCCGgc   >  2:2652351/1‑101 (MQ=21)
                                                                                      ggggTGAAGCCCC...C.C....GCGGGG...CG....CCTCGGGCTTGCCCGAGGCGTAAGCTTCCCCCCGTGGAAACCTTGCCTGCGCGCGTCGCTTTCCCTTGCGGCCCGgcg  >  2:1681885/1‑101 (MQ=21)
                                                                                                         |                                                                                                
CTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.C....GT...C...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGGCG  >  NC_014034/1732766‑1732949

Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 20 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG