BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,867,831	+C	5.8%	intergenic (‑233/+82)	RCAP_rcc01729 ← / ← RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,867,831	1	.→C	5.8%	8.7 ‑2.6 ‑0.0	86	intergenic (‑233/+82)	RCAP_rcc01729/RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase
Reads supporting (aligned to +/- strand): new base (0/5): ref base (35/46): total (35/51)
Fisher's exact test strand distribution p-value = 7.68e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.32e-01

Only 100 of 214 total aligned reads displayed.


AGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CG.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867731‑1867928


 ggAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.g.c                                                                                                       <  1:3209114/101‑1 (MQ=255)


 ggAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.g.c                                                                                                       <  1:474448/101‑1 (MQ=255)


    aaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCACCCCCGGCGGC.GCGCA.A.G.cg.cc                                                                                                   >  2:2427977/1‑101 (MQ=38)


     aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.ccc                                                                                                  <  1:5758334/101‑2 (MQ=255)


     aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.ccc                                                                                                  <  2:3505890/101‑2 (MQ=255)


     aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.ccc                                                                                                  <  2:3680965/101‑2 (MQ=255)


     aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.ccc                                                                                                  <  2:3127680/101‑2 (MQ=255)


     aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCCGCGGC.GCGGA.A.G.cg.ccc                                                                                                  >  1:4141147/1‑100 (MQ=37)


      aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.cccg                                                                                                 <  1:2027748/101‑3 (MQ=255)


      aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCACCCCCGGCGGC.GCGCA.A.G.cg.cccc                                                                                                 >  1:3685932/1‑99 (MQ=255)


         aCTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.cccgcca                                                                                              <  1:4411581/101‑6 (MQ=255)


          cTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCCCCCCCGCGGC.GCGGA.A.G.Cg.cccccccc                                                                                             >  1:5375062/1‑98 (MQ=25)


           tGCTTTCCCCCGCCCGGATCGCGACAGCTTTGCAAAGCCCGCACCCCCAAGCGCGCGAGGGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATg                                                                                             <  2:5200466/101‑1 (MQ=21)


             cTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCCGCCCCCGCCGC.GCGAATA.C.GG.CCGCCCTggg                                                                                           >  2:3846708/1‑101 (MQ=25)


              ttttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCCGCGGC.GCGCA.A.G.cg.cccgcccccggc                                                                                         >  1:4694062/1‑91 (MQ=25)


              tttcCCCGGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATgggc                                                                                          <  2:4727713/101‑1 (MQ=21)


                ttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACCCCCAGCCCCCGCGGG.GCGAA.A.A.CGCCCGCCATgggc.gg                                                                                       >  1:4184632/1‑101 (MQ=255)


                ttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCAGCCCCCGCGGC.GCGAA.A.G.Cg.ccccccccgggcgg                                                                                       >  2:817460/1‑92 (MQ=25)


                   cccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATgggc.ggggc                                                                                    <  1:3656287/101‑1 (MQ=255)


                   cccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATgggc.ggggc                                                                                    <  1:628330/101‑1 (MQ=255)


                    ccgcccgTGTCGCGACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATgggc.ggggcg                                                                                   <  2:95047/101‑1 (MQ=255)


                    ccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATgggc.ggggcg                                                                                   <  1:1740219/101‑1 (MQ=255)


                     cgcccgTGTCGCGACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGggc.ggggcgg                                                                                  <  1:4416763/101‑1 (MQ=21)


                     cgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCATAGCCCAACCCCCGCCGCGGCGAA.A.C.GG.CCGCCACGggc.ggggggg                                                                                  >  2:5742988/1‑101 (MQ=255)


                      cccggtgaCGCGAGAGCGTTGCAAAGCCCCCACCCCAAGGCGCGCGAGAGCCCCCCGCCCGGCCCCGGGGGC.GCGAA.A.G.CGCCCGCCATGGgc.ggggcggg                                                                                 <  2:1388133/97‑1 (MQ=12)


                       cccgTGTCGCGACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.Ggggcgggc                                                                                <  1:2543337/101‑1 (MQ=255)


                       cccgTGTCGCGACAGCGTGGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.Ggggcgggc                                                                                <  2:1871563/101‑1 (MQ=255)


                        ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCCTGGgc.gggggggggg                                                                               >  1:881706/1‑99 (MQ=21)


                         cgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGgcgc                                                                              <  1:5577378/101‑1 (MQ=255)


                          gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGC.GCGAA.A.G.CGCCCCCCCTGGGC.GGGGCGGgggcc                                                                             >  1:2603011/1‑100 (MQ=18)


                           tgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCCCGGGC.GGGGCGGgcgccg                                                                            >  1:513177/1‑99 (MQ=18)


                            gtCGCGACGGCGTTGAAAAGACCGCACCCCCAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.gg                                                                          <  2:3190345/101‑1 (MQ=17)


                            gacgcgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGgc.gggggggggggcgg                                                                           >  1:3845525/3‑95 (MQ=18)


                              cgcgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGC.GCGCA.A.G.CGCCCGCCCCGGGC.GGgggcggcggcgggc                                                                         >  2:2356954/1‑95 (MQ=18)


                               gcgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCg                                                                       <  1:3378293/101‑1 (MQ=255)


                               gcgACAGCGTTGAAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCg                                                                       <  2:2632907/101‑1 (MQ=17)


                                cgACAGCGTGGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGa                                                                      <  2:2720487/101‑1 (MQ=17)


                                 gtcAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.g                                                                    <  2:5073743/99‑1 (MQ=255)


                                 gACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCAGCCCCGGCGGC.GCGAA.A.A.CGCCCGCCATGGGC.GGGGGGGGGGCG.GGcccgg                                                                     >  2:5167143/1‑98 (MQ=18)


                                  aCAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGC.GCGAA.A.G.CGCCCGCCATGGGG.GGGGCGGGGGCC.GGGCGacg                                                                    >  2:402570/1‑99 (MQ=18)


                                   cAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGC.GCGGA.A.G.CGCCCGCCCTCGGG.GGGGCGGGGGCG.GGGCGG.ggg                                                                  >  2:5426169/1‑101 (MQ=18)


                                     gCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCCTGGGC.GGGGCGGGGGCC.GGGCGG.ggggc                                                                >  1:3458593/1‑99 (MQ=18)


                                      cGTTGCAAAGCCCGCACCCCCAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCt                                                              <  2:4140897/101‑1 (MQ=17)


                                       tttGCAAAGACCGCACCGCCAAGCGCGCGAGAGGCGGGCCCCCGGCCCGGGCGGC.GCGAA.A.C.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCtt                                                             <  2:1301448/100‑1 (MQ=17)


                                       gTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGACCCGGCGGC.GCGAA.A.A.CGCCCGCGCTGGGC.GGGGCGGGGGCG.GGCCCA.GGG.GCtt                                                             >  2:5157418/1‑101 (MQ=17)


                                        ttGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCttt                                                            <  1:2428111/101‑1 (MQ=17)


                                         tGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCCCCCCCGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCG.GGGCGA.GGG.CCcttt                                                           >  1:2773537/1‑100 (MQ=14)


                                          gCAAGGCCCGCACCCCCAAGCGCGCGGGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTgg                                                          <  2:4693781/101‑1 (MQ=14)


                                          gCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCCTGGGC.GGGGCGGGGGCC.GGGCGAGGGG.CCTTTg                                                           >  1:311082/1‑101 (MQ=17)


                                            aaaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCG.GGCCGG.GGG.GCCTTgggg                                                        >  2:3961847/1‑101 (MQ=17)


                                             aaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCC.GGGCGG.GGG.CCGTTggggg                                                       >  1:1274758/1‑100 (MQ=17)


                                              agcccGCACCCCCAAGCGCGCGAGGGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGcc                                                      <  2:881630/98‑1 (MQ=17)


                                              aGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCGCAGCCCCCGCGGG.GCCAA.A.G.CGCCCGCCCAGGGC.GGGGTGGGGCCT.CGACCA.GGG.cccctttggcc                                                      >  2:1585928/1‑92 (MQ=255)


                                              aGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGGGGGGGCT.GGCCGG.GGG.CGCTTGTGGcg                                                      >  2:1208574/1‑100 (MQ=14)


                                              aGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGC.GCGAA.A.G.CGCCCGCCCTGGGC.GGGGGGGGCGCC.GGCCGA.GGG.GCTTTtggggc                                                      >  2:2242351/1‑99 (MQ=17)


                                               gACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCCGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCC.GGCCGA.GGGCCCTTTGGGGcc                                                      >  2:4291353/1‑101 (MQ=14)


                                               gACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCTGGGCCGG.GGG.CGTTGGGGGcc                                                      >  2:859556/1‑101 (MQ=14)


                                                 ccGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGccccc                                                   <  1:2721245/101‑1 (MQ=255)


                                                 ccGCACCCCCAAGCGCGCGAGAGCCCGGCCCCCAGCCCCGGCGCC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGccccc                                                   <  2:4786473/101‑1 (MQ=17)


                                                    cccccccAAGCGCGGGAGGGGCCAGCCCCCAGCCCCGGCGGG.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCcggc                                                <  2:4476927/98‑1 (MQ=14)


                                                    cATCCCCAAGCGCGCGAGAGGCCAGCCCCAAGCCCCGGCGGC.GCGAA.AGG.CC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCcggc                                                <  1:847832/101‑1 (MQ=18)


                                                     ctCCCCAAGCGGCCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.G.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCcggcg                                               <  2:4381767/100‑1 (MQ=14)


                                                     aTCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGGT.GGCCGC.GGG.CCTTTGGGGCCCCccgcg                                               >  1:660978/1‑101 (MQ=18)


                                                     aTCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCT.GGCCGAGGGG.CCTTGGGGGCCCCcgcg                                                >  2:3252332/1‑99 (MQ=17)


                                                       ccccAGGCGCGGGAGGGGCCGGCCCCCCGCCCCGGCGGC.GCAAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGt                                             <  1:4062110/101‑1 (MQ=14)


                                                         ccAGGCGCGCGAGAGGCCAGCGCCCAGCCCGGGCGGC.GCGAA.G.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCa                                           <  1:5536299/101‑1 (MQ=17)


                                                          caggcgcgcGAGAGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAg                                          <  1:5314789/98‑1 (MQ=17)


                                                          cAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCC.GGCCGG.GGG.CCCTTTGGGCCCCccgcgggccg                                          >  1:1119381/1‑97 (MQ=14)


                                                          cAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCC.GGGCGA.GGG.GCCTTGGGGCCCCCCGCGGGCAg                                          >  1:278721/1‑101 (MQ=17)


                                                           acgcgcgcGAGGGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAgg                                         <  1:806986/99‑1 (MQ=17)


                                                           aaGCGCGCGAGAGCCCAGCCCCCAGCCCGGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAgg                                         <  2:660921/101‑1 (MQ=18)


                                                             gcgcgcCAGAGCCCAGCCCCCCGCCCCGGCGGC.GCAAA.ACC.CC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAgggg                                       <  1:923494/101‑1 (MQ=14)


                                                               gtgacagagGCCTGCGCCCAGCCCCCGCGGC.GCGAA.A.G.CGCCCCCCCTGGGCGGGGGGGGGGGGG.GGCGGG.GGG.CCTTTGGGCCCCGCGGGGGGCGggggg                                      >  2:4991132/6‑101 (MQ=2)


                                                               gggcgAGAGCCCAGCGCCCAGCCCCGGCGGC.GCGAA.G.C.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGa                                     <  2:3409397/99‑1 (MQ=17)


                                                               gcgcgAGAGGCCAGCGCCCGGGCCGGGCGGG.GGGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGa                                     <  1:3622721/101‑1 (MQ=17)


                                                               gcgcgAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCC.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGGCCgggggg                                     >  2:5473522/1‑100 (MQ=17)


                                                                 gcgAGAGGCCAGCGCCCAGCCCCGGCGGC.GCAAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATc                                   <  1:1874560/101‑1 (MQ=17)


                                                                  ggggagGCCAGCGCCCACCCCCGGGGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCt                                  <  2:1428900/98‑1 (MQ=17)


                                                                   gagaGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCCCCATGGGC.GGGGCGGGCGCG.GGGCGA.GGG.CCTTTGTGGTCCCCCGCGGTTCgggggcgttt                                 >  1:4827970/1‑96 (MQ=11)


                                                                    ggagGCCAGCGCCCAGCCCCGGCGGC.GCGAA.G.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGt                                <  1:3893242/100‑1 (MQ=255)


                                                                     gagGCCACCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                               <  2:622507/101‑1 (MQ=17)


                                                                       ggCCAGCCCCCAGCCCCGGCGGC.GCGAA.G.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgc                             <  2:1980695/101‑1 (MQ=18)


                                                                         ccAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGGTCTGTTgcgc                           >  2:4948017/1‑101 (MQ=255)


                                                                           ggcgcCCGGCCCGGGGGGC.GCGAA.G.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                         <  1:5028292/100‑1 (MQ=17)


                                                                           cgcgcCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                         <  1:402614/100‑1 (MQ=255)


                                                                              gcCCAGCCCGGGGGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGACGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGc                      <  1:5732352/101‑1 (MQ=18)


                                                                              gcCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGc                      >  1:116517/1‑101 (MQ=255)


                                                                                 cAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgc                   >  2:1749954/1‑101 (MQ=255)


                                                                                  cgCCCCGGCGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgcg                  <  2:322647/100‑1 (MQ=255)


                                                                                     cccGGGGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  2:4533851/101‑1 (MQ=255)


                                                                                     cccGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               >  2:4207026/1‑101 (MQ=255)


                                                                                       cggcggC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgc             >  1:1430004/1‑101 (MQ=255)


                                                                                        ggcggc.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgcg            <  1:2229769/101‑1 (MQ=255)


                                                                                          cggc.gCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATTGCCGCGTCAGcgcc          >  2:2885604/1‑101 (MQ=255)


                                                                                             c.gcaAN.N.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCcgcg       <  1:2490114/101‑1 (MQ=255)


                                                                                                cgAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGc     >  2:463402/1‑101 (MQ=255)


                                                                                                cgAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGc     >  1:5225214/1‑101 (MQ=255)


                                                                                                 gAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGcc    >  2:2904690/1‑101 (MQ=255)


                                                                                                 gAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGcc    <  2:3570112/101‑1 (MQ=255)


                                                                                                   a.a.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCaa  >  1:3155423/1‑101 (MQ=255)


AGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CG.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867731‑1867928

Base quality scores:


ATCG

< 3 ≤


ATCG

< 12 ≤


ATCG

< 21 ≤


ATCG

< 32 ≤


ATCG

< 41 ≤


ATCG