BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,867,833	+C	100%	intergenic (‑235/+80)	RCAP_rcc01729 ← / ← RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,867,833	1	.→C	100.0%	40.4	120	intergenic (‑235/+80)	RCAP_rcc01729/RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase
Reads supporting (aligned to +/- strand): new base (35/71): ref base (0/14): total (35/85)
Fisher's exact test strand distribution p-value = 9.76e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.38e-01

Only 100 of 209 total aligned reads displayed.


GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CG.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867733‑1867928


  aaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAACGCCCAGCCCCCGCGGG.GCGCA.A.G.cg.cc                                                                                                   >  2:3825976/1‑101 (MQ=38)


   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.ccc                                                                                                  <  1:5758334/101‑2 (MQ=255)


   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAACGCCCCGCCCCCGCGGC.GCGCA.A.A.cg.ccc                                                                                                  >  1:782117/1‑100 (MQ=37)


    aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.cccg                                                                                                 <  1:2027748/101‑3 (MQ=255)


    aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCACCCCCGGCGGC.GCGCA.A.G.cg.cccc                                                                                                 >  1:3685932/1‑99 (MQ=255)


      cACTGCTTTTCCCCGCCCGTGTCGCGATAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.cccgcc                                                                                               <  2:1856699/101‑5 (MQ=255)


      cACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.cccgcc                                                                                               <  1:4222677/101‑5 (MQ=255)


       aCTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.cg.cccgcca                                                                                              <  1:4411581/101‑6 (MQ=255)


        cTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGGATCCCCAAG.CGCG.CGAGAGGCCAAAGCCCAGCCCCCGCGGC.GCGAA.A.G.Cg.ccccccac                                                                                             >  2:2951776/1‑98 (MQ=37)


        cTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCCCCCCCCGCGGC.GCGGA.A.G.Cg.cccccccc                                                                                             >  1:5375062/1‑98 (MQ=25)


          gCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATgg                                                                                            <  2:3557316/101‑1 (MQ=255)


           cTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAACGCCCACCCCCCCCGGC.GCGAA.A.G.Cg.ccccccccggg                                                                                          >  1:4818512/1‑95 (MQ=37)


           cTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAAAGCCCCGCCCCCGCCGC.GCGAATA.C.GG.CCGCCCTggg                                                                                           >  2:3846708/1‑101 (MQ=25)


            ttttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCCGCCCCCGCGGC.GCGCA.A.G.cg.cccgcccccggc                                                                                         >  1:4694062/1‑91 (MQ=25)


            tttcCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATgggc                                                                                          <  2:2894263/101‑1 (MQ=255)


              ttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAAAGCCCAGCCCCCGCGGC.GCGAA.A.G.Cg.ccccccccgggcgg                                                                                       >  2:817460/1‑92 (MQ=25)


                ccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCCGCCGC.GCGCA.A.G.CGCCCGCCCTcggc.gggg                                                                                     >  1:594508/1‑101 (MQ=25)


                  ccgcccgTGTCGCGACAGCGTTGCAAAGCCCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATgggc.ggggcg                                                                                   <  2:95047/101‑1 (MQ=255)


                  ccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATgggc.ggggcg                                                                                   <  1:1740219/101‑1 (MQ=255)


                   cgcccgTGTCGCGACAGCGTTGCAAAGCCCGCATCCCCAAG.CGCG.CGAGAGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGggc.ggggcgg                                                                                  <  1:4416763/101‑1 (MQ=21)


                   cgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCATAGCCCAACCCCCGCCGCGGCGAA.A.C.GG.CCGCCACGggc.ggggggg                                                                                  >  2:5742988/1‑101 (MQ=255)


                     ccggggtCGCGACAGCGTTGAAAAGCCCCCACCCCCAGG.CGCG.GGAGAGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CCCCCGCCATGGGC.Ggggcgggc                                                                                <  1:4039613/96‑1 (MQ=17)


                     cccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAACGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGgc.ggggcgggg                                                                                >  2:2373891/1‑100 (MQ=255)


                      ccgTGTCGCTACAGCGTTGCAAAGACCGCACCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGggcgggcg                                                                               <  2:1879220/101‑1 (MQ=18)


                      ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCCTGGgc.gggggggggg                                                                               >  1:881706/1‑99 (MQ=21)


                       cgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGgcgc                                                                              <  1:5577378/101‑1 (MQ=255)


                        gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCt                                                                             <  2:3415721/101‑1 (MQ=255)


                         tgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAACGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCCCGGGC.GGGGCGGgcgccg                                                                            >  1:513177/1‑99 (MQ=18)


                         tgtCGCGAAAGCGTTGCAAAGCCCGCACCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.g                                                                           <  2:2461585/101‑1 (MQ=18)


                          gtCGCGACGGCGTTGAAAAGACCGCACCCCCAGG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.gg                                                                          <  2:3190345/101‑1 (MQ=17)


                            cgcgACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAACGCCCAGCCCCCGCGGC.GCGAA.A.A.CGCCCCCCCTGGGC.GGGGGGGGGGCC.GGcc                                                                        >  1:4100271/1‑101 (MQ=18)


                             cggACAGCGTTGCAAAGCCCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCGGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCg                                                                       <  2:5592565/99‑1 (MQ=17)


                               gtcAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.g                                                                    <  2:5073743/99‑1 (MQ=255)


                                aCAGCGTTGCAAAGGCCGCATCCCCAAG.CGCG.CGAGAGCCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.gg                                                                   <  1:2782370/101‑1 (MQ=17)


                                aCAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAACGCCCAGCCCCCGCGGC.GCGAA.A.G.CGCCCGCCATGGGG.GGGGCGGGGGCC.GGGCGacg                                                                    >  2:402570/1‑99 (MQ=18)


                                    cGTTGCAAAGCCCGCACCCCCAGG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCt                                                              <  2:4140897/101‑1 (MQ=17)


                                     gTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGACCCGGCGGC.GCGAA.A.A.CGCCCGCGCTGGGC.GGGGCGGGGGCG.GGCCCA.GGG.GCtt                                                             >  2:5157418/1‑101 (MQ=17)


                                      tggCAAAGACCTCATCCCTAGG.GGCG.CGAGTGGCAATCCCCCCGCCCAGCCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGCGCGGGCGCT.GGCCGA.GGG.CCttt                                                            <  2:2267121/99‑1 (MQ=9)


                                        gCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCCTGGGC.GGGGCGGGGGCC.GGGCGAGGGG.CCTTTg                                                           >  1:311082/1‑101 (MQ=17)


                                          aaaGCCCCCACCCCCAGG.CGCG.CGAGAGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTgggg                                                        <  2:4402660/101‑1 (MQ=17)


                                          aaaGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCCGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCG.GGCCGG.GGG.GCCTTgggg                                                        >  2:3961847/1‑101 (MQ=17)


                                           acgACCGCATCCCCGAG.CACGACGAGAGGCCAGCGCCCAGCCCCGGCGGG.CGGGA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCG.GGCCGG.GGC.CCctttgcg                                                        >  1:2478487/3‑97 (MQ=18)


                                           aaGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCC.GGGCGG.GGG.CCGTTggggg                                                       >  1:1274758/1‑100 (MQ=17)


                                            agcccGCACCCCCAAG.CGCG.CGAGGGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGcc                                                      <  2:881630/98‑1 (MQ=17)


                                            aGACCGCATCCCCAAG.CGCG.CGAGAGGCCAACGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGGGGGGGCT.GGCCGG.GGG.CGCTTGTGGcg                                                      >  2:1208574/1‑100 (MQ=14)


                                            aGACCGCATCCCCAAG.CGCG.CGAGAGGCCAACGCCCAGCCCCCGCGGC.GCGAA.A.G.CGCCCGCCCTGGGC.GGGGGGGGCGCC.GGCCGA.GGG.GCTTTtggggc                                                      >  2:2242351/1‑99 (MQ=17)


                                             gACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCCGCCCCCGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCC.GGCCGA.GGGCCCTTTGGGGcc                                                      >  2:4291353/1‑101 (MQ=14)


                                             gACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCTGGGCCGG.GGG.CGTTGGGGGcc                                                      >  2:859556/1‑101 (MQ=14)


                                               ccGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCCGCCCCGGCGGC.GCCAA.A.C.CGCCCGCCATGGGC.GGGGGGGGGGCT.GGCCCA.GGGCCCTTTGGGGcccc                                                    >  1:3643050/1‑101 (MQ=14)


                                               ccGCACCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGccccc                                                   <  1:2721245/101‑1 (MQ=255)


                                                 cgcaccccAAGCCGCG.CGAGGGGCCAGCGCCCACCCCCGGCGGC.GCAAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCcg                                                  <  2:4960036/97‑1 (MQ=17)


                                                  cgcccccACG.CGCG.GGGGGGGCCCGCGCCCAGCCCGGGCGGC.GCGAA.G.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCcggc                                                <  1:3878833/98‑1 (MQ=14)


                                                  cccccccAAG.CGCG.GGAGGGGCCAGCCCCCAGCCCCGGCGGG.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCcggc                                                <  2:4476927/98‑1 (MQ=14)


                                                  cATCCCCAAG.CGCG.CGAGAGGCCAGCCCCAAGCCCCGGCGGC.GCGAA.AGG.CC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCcggc                                                <  1:847832/101‑1 (MQ=18)


                                                      cccAGG.CGCG.GGGGAGGCCAGCCCCCAGCCCCGGGGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTc                                            <  1:3602744/101‑1 (MQ=14)


                                                       ccAGG.CGCG.CGAGAGGCCAGCGCCCAGCCCGGGCGGC.GCGAA.G.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCa                                           <  1:5536299/101‑1 (MQ=17)


                                                       ccAAG.CGCG.GGAGAGGCCGGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCAGGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCa                                           <  1:2532969/101‑1 (MQ=14)


                                                        cagg.cgcg.cGAGAGGCCAGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAg                                          <  1:5314789/98‑1 (MQ=17)


                                                        cAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCCTGGGC.GGGGCGGGCGCC.GGCCCA.GGG.CCTTTTGGGGCCCccgcgggccg                                          >  1:4159557/1‑97 (MQ=14)


                                                        cAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGGGCC.GGCCGG.GGG.CCCTTTGGGCCCCccgcgggccg                                          >  1:1119381/1‑97 (MQ=14)


                                                         aaG.CGCG.CGAGAGCCCAGCCCCCAGCCCGGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAgg                                         <  2:660921/101‑1 (MQ=18)


                                                           g.cgcg.cCAGAGCCCAGCCCCCCGCCCCGGCGGC.GCAAA.ACC.CC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAgggg                                       <  1:923494/101‑1 (MQ=14)


                                                             cgcc.cgGGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAggggg                                      <  2:4818231/101‑1 (MQ=17)


                                                               gtgacagagGCCTGCGCCCAGCCCCCGCGGC.GCGAA.A.G.CGCCCCCCCTGGGCGGGGGGGGGGGGG.GGCGGG.GGG.CCTTTGGGCCCCGCGGGGGGCGggggg                                      >  2:4991132/6‑101 (MQ=2)


                                                               gg.cgAGAGGCCCGCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGAt                                    <  2:1703727/100‑1 (MQ=17)


                                                                g.cgAGAGGCCAGCGCCCAGCCCCGGCGGC.GCAAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATc                                   <  1:1874560/101‑1 (MQ=17)


                                                                g.cgAGAGCCCAGCCCCCAGCCCGGGCGGC.GCAAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATc                                   <  2:3133754/101‑1 (MQ=17)


                                                                  cgAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGGCCCTTTGGGGCCCCC.GCGGGCCGGGGGGTCt                                  >  1:628491/1‑101 (MQ=14)


                                                                    agagGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.A.CGCCCGCCATGGGC.GGGGCGGGCGCC.GGCCGA.GGG.GCCTTGGGGCCCCCCGCGGGCCGGGGGGGCTGt                                >  2:5178960/1‑101 (MQ=14)


                                                                     gagGCCTGCGCCCAGCCCCGGCGGC.GCGAA.T.G.CGCCCGCCATGGGC.GGGGCGGGGGGC.GGCCCG.GGG.CCTTTTTGGCCCCCGGGGGGCTgggggtgaaagg                               >  2:4695756/1‑94 (MQ=2)


                                                                     gagGCCACCCCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                               <  2:622507/101‑1 (MQ=17)


                                                                      agGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGG.GGG.GCTTTTGGGCCCCCGGCGGTCAGGGGGacccggtg                              >  2:2717585/1‑94 (MQ=14)


                                                                      agGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGGCAGGGGGGTCTGTTg                              >  1:491834/1‑101 (MQ=17)


                                                                         ccAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgcgc                           >  2:3405062/1‑101 (MQ=255)


                                                                           aGCGCCCAGCCCGGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                         <  2:5252985/101‑1 (MQ=255)


                                                                             cccccACCCCCGGCGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATgg                       <  2:1538737/99‑1 (MQ=18)


                                                                              gcCCAGCCCGGGGGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGACGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGc                      <  1:5732352/101‑1 (MQ=18)


                                                                              gcCCAGCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGc                      >  1:116517/1‑101 (MQ=255)


                                                                                 cAGCCCGGGCGGC.GCGAA.G.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgc                   <  2:3637805/101‑1 (MQ=18)


                                                                                  cgCCCCGGCGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgcg                  <  2:322647/100‑1 (MQ=255)


                                                                                  cgCCCCGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgcg                  <  2:124520/100‑1 (MQ=255)


                                                                                    ccacggcggC.GCGAA.A.G.CGCCCGCCATGGAC.GGACCGGGCGCT.CGTCGA.GGG.CCATTTGGGCCCCCGGCGGGCACGGGGATCTGTTGCGCATGGCCGCGTc                >  2:4772343/4‑101 (MQ=17)


                                                                                     cccGGGGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  2:4533851/101‑1 (MQ=255)


                                                                                     cccGGGGGC.GCAAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  1:4002105/101‑1 (MQ=21)


                                                                                     cccGGCGGC.GCGAA.A.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgcgcta               <  1:3300473/101‑4 (MQ=18)


                                                                                     cccGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               >  2:4207026/1‑101 (MQ=255)


                                                                                     cccGGCGGC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  1:2312729/101‑1 (MQ=255)


                                                                                       cgggggC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgc             <  1:3405267/101‑1 (MQ=255)


                                                                                       cggcggC.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgc             >  1:1430004/1‑101 (MQ=255)


                                                                                        ggcggc.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgcg            >  1:3671571/1‑101 (MQ=255)


                                                                                        ggcggc.GCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgcg            <  1:2229769/101‑1 (MQ=255)


                                                                                          gggc.gCAAA.C.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgcc          <  1:2570387/100‑1 (MQ=21)


                                                                                          cggc.gCGAA.G.GCCC.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgcc          <  2:1366042/101‑1 (MQ=21)


                                                                                           ggc.gCGAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgccg         >  2:1424831/1‑101 (MQ=255)


                                                                                                cgAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGc     >  1:5225214/1‑101 (MQ=255)


                                                                                                cgAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGc     >  2:463402/1‑101 (MQ=255)


                                                                                                cgAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCCGc     >  1:287860/1‑101 (MQ=255)


                                                                                                cgAA.A.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTCGTGCCCCCGGCGGTCAGCGGGATCTGTTGCGCATGGGCGCGTGAGCGCCGCGGc     >  2:2771035/1‑101 (MQ=18)


                                                                                                   a.a.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCaa  >  1:3155423/1‑101 (MQ=255)


                                                                                                   a.a.G.CGCCCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCaa  >  2:543811/1‑101 (MQ=255)


GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAG.CGCG.CGAGAGGCCAGCGCCCAGCCCCGGCGGC.GCGAA.A.G.CG.CCGCCATGGGC.GGGGCGGGCGCT.GGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867733‑1867928

Base quality scores:


ATCG

< 3 ≤


ATCG

< 14 ≤


ATCG

< 21 ≤


ATCG

< 32 ≤


ATCG

< 41 ≤


ATCG