BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,997,981	+G	96.1%	intergenic (‑46/‑119)	RCAP_rcc01844 ← / → RCAP_rcc01845	sensor histidine kinase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,997,981	1	.→G	96.1%	24.4 ‑0.6 ‑1.2	279	intergenic (‑46/‑119)	RCAP_rcc01844/RCAP_rcc01845	sensor histidine kinase/hypothetical protein
Reads supporting (aligned to +/- strand): new base (125/143): ref base (4/7): total (129/150)
Fisher's exact test strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.05e-02

Only 100 of 303 total aligned reads displayed.


GCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGTTTC  >  NC_014034/1997881‑1998077


gCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTc                                                                                                   <  1:4105081/101‑1 (MQ=255)


gCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTc                                                                                                   >  2:5186845/1‑101 (MQ=255)


gCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTc                                                                                                   <  2:17964/101‑1 (MQ=255)


  aGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.gg                                                                                                <  1:1132525/101‑1 (MQ=255)


  aGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.gg                                                                                                >  2:3446381/1‑101 (MQ=255)


  aGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.gg                                                                                                >  1:2225253/1‑101 (MQ=255)


   gAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.ggg                                                                                               <  2:2826395/101‑1 (MQ=255)


      tCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCCCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGgaa                                                                                            <  2:3669853/101‑1 (MQ=255)


      tCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGgaa                                                                                            <  2:114608/101‑1 (MQ=255)


      tCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGgaa                                                                                            <  2:1965025/101‑1 (MQ=255)


        ggAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCCTC.GGGgaaac                                                                                          >  2:4617717/1‑99 (MQ=255)


           aaCGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGAt                                                                                        >  1:868219/1‑101 (MQ=255)


           aaCGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGAt                                                                                        <  1:1493016/101‑1 (MQ=255)


            aCGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATa                                                                                       <  2:822943/101‑1 (MQ=255)


             cGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATAc                                                                                      >  1:3025422/1‑101 (MQ=255)


              ggTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGCCCCGAGCAAGCTTCGGGGCAACGATAcc                                                                                     >  1:5321565/1‑101 (MQ=255)


               gTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATAccc                                                                                    >  2:5207866/1‑101 (MQ=255)


                tAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCa                                                                                   >  1:1983336/1‑101 (MQ=255)


                tAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCa                                                                                   <  1:4011120/101‑1 (MQ=255)


                 aaCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCaa                                                                                  >  2:2191575/1‑101 (MQ=255)


                  aCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAg                                                                                 <  1:3782237/101‑1 (MQ=255)


                  aCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAg                                                                                 <  1:2756950/101‑1 (MQ=255)


                    gTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGtt                                                                               >  2:1231516/1‑101 (MQ=255)


                    gTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGtt                                                                               >  2:654150/1‑101 (MQ=255)


                     tctcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTc                                                                              <  2:4912090/101‑1 (MQ=255)


                      ctcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCa                                                                             >  2:4205532/1‑101 (MQ=255)


                       tcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCaa                                                                            >  1:2846655/1‑101 (MQ=255)


                       tcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCaa                                                                            <  2:3319871/101‑1 (MQ=255)


                       tcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCaa                                                                            <  1:1814946/101‑1 (MQ=255)


                        cGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAg                                                                           >  1:5064307/1‑101 (MQ=255)


                        cGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAg                                                                           >  2:2095738/1‑101 (MQ=255)


                         ggCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAgg                                                                          >  1:5697039/1‑101 (MQ=255)


                            cAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCg                                                                       >  1:5416714/1‑101 (MQ=255)


                            cAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCg                                                                       >  2:4021376/1‑101 (MQ=255)


                              aGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCa                                                                     >  2:485065/1‑101 (MQ=255)


                              aGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCa                                                                     <  1:1008171/101‑1 (MQ=255)


                                 agaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGc                                                                  <  1:4293275/101‑1 (MQ=255)


                                    ttgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaa                                                               <  1:1634127/101‑1 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                              <  2:5416424/101‑1 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                              <  2:374531/101‑1 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                              <  2:3135457/101‑1 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAg                                                             >  2:2330590/1‑101 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAg                                                             <  1:5230983/101‑1 (MQ=255)


                                        tgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGATTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGcc                                                           >  2:1520964/1‑101 (MQ=255)


                                            gAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCTGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAggg                                                       <  1:4869169/101‑1 (MQ=255)


                                             aaTCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAgggg                                                      >  1:2634674/1‑101 (MQ=255)


                                              aTCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAggggg                                                     >  2:3344128/1‑101 (MQ=255)


                                                  gCCATTTTTCGGTCCCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATGCCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTa                                                 <  2:1625844/101‑1 (MQ=255)


                                                  gCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTa                                                 <  1:2958842/101‑1 (MQ=255)


                                                   ccATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTaa                                                >  2:3959235/1‑101 (MQ=255)


                                                    cATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAg                                               <  1:4205792/101‑1 (MQ=255)


                                                     aTTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGc                                              <  1:3424043/101‑1 (MQ=255)


                                                      tttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCt                                             <  2:2634525/101‑1 (MQ=255)


                                                      tttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCt                                             <  2:5064023/101‑1 (MQ=255)


                                                       ttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtt                                            >  1:3883829/1‑101 (MQ=255)


                                                       tttgCGGTCTCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtt                                            <  1:5517252/101‑1 (MQ=255)


                                                        tttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCttt                                           <  2:4442978/101‑1 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                          >  1:5353315/1‑101 (MQ=255)


                                                            ggTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCt                                       >  1:3681019/1‑101 (MQ=255)


                                                            ggTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCt                                       >  1:591585/1‑101 (MQ=255)


                                                             gTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTg                                      <  2:2079016/101‑1 (MQ=255)


                                                              tCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGa                                     <  1:3476493/101‑1 (MQ=255)


                                                               cACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGaa                                    <  1:2340750/101‑1 (MQ=255)


                                                               cACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGaa                                    >  1:741334/1‑101 (MQ=255)


                                                                 cccTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAAtt                                  <  1:2145667/101‑1 (MQ=255)


                                                                 cccTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAAtt                                  <  1:3930121/101‑1 (MQ=255)


                                                                  ccTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTc                                 <  1:3157004/101‑1 (MQ=255)


                                                                   cTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTcg                                <  1:4333688/101‑1 (MQ=255)


                                                                    tGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTcgc                               >  2:5306537/1‑101 (MQ=255)


                                                                     gACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTcgcg                              >  2:1332246/1‑101 (MQ=255)


                                                                      aCATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCgg                             >  1:4813615/1‑101 (MQ=255)


                                                                        atatGCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATAGCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                           <  2:5698829/101‑1 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                           >  2:267584/1‑101 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                           >  1:105657/1‑101 (MQ=255)


                                                                         tatCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtg                          >  2:2929925/1‑101 (MQ=255)


                                                                           tCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgtt                        >  1:5628467/1‑101 (MQ=255)


                                                                           tCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgtt                        <  2:3025248/101‑1 (MQ=255)


                                                                            ccAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttg                       <  1:4433067/101‑1 (MQ=255)


                                                                            ccAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttg                       >  1:955173/1‑101 (MQ=255)


                                                                             cAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgt                      <  1:2319811/101‑1 (MQ=255)


                                                                              aaTGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgtt                     >  1:4535650/1‑101 (MQ=255)


                                                                              aaTGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGACGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgtt                     >  2:2142204/1‑101 (MQ=255)


                                                                               aTGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgttg                    >  1:1440663/1‑101 (MQ=255)


                                                                               aTGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgttg                    <  1:272929/101‑1 (MQ=255)


                                                                                tGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgttgt                   <  1:1186682/101‑1 (MQ=255)


                                                                                 gCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTc                  <  2:4337648/101‑1 (MQ=255)


                                                                                    tGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATg               <  1:1231599/101‑1 (MQ=255)


                                                                                    tGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATg               <  2:5353025/101‑1 (MQ=255)


                                                                                    tGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATg               <  2:5580927/101‑1 (MQ=255)


                                                                                    tGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATg               <  1:250901/101‑1 (MQ=255)


                                                                                    tGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATg               >  1:3080163/1‑101 (MQ=255)


                                                                                      aCCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAg             >  2:1908436/1‑101 (MQ=255)


                                                                                        ccGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCg           <  2:1384188/101‑1 (MQ=255)


                                                                                        ccGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCg           >  2:725304/1‑101 (MQ=255)


                                                                                         cGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGa          <  2:1662697/101‑1 (MQ=255)


                                                                                            gCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATgg       <  2:3854507/101‑1 (MQ=255)


                                                                                             cAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATggg      >  1:3271558/1‑101 (MQ=255)


                                                                                               aGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGaattcag    <  2:35348/101‑7 (MQ=255)


                                                                                               aGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGtt    >  1:1310063/1‑101 (MQ=255)


                                                                                                gCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGttt   >  1:1755251/1‑101 (MQ=255)


GCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGTTTC  >  NC_014034/1997881‑1998077

Base quality scores:


ATCG

< 3 ≤


ATCG

< 25 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG