BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,047,090	+C	89.9%	coding (20/879 nt)	potH1 ←	polyamine ABC transporter permease PotH

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,047,090	1	.→C	89.9%	90.2 ‑2.5 ‑1.1	297	coding (20/879 nt)	potH1	polyamine ABC transporter permease PotH
Reads supporting (aligned to +/- strand): new base (122/145): ref base (19/11): total (141/156)
Fisher's exact test strand distribution p-value = 8.27e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.84e-02

Only 100 of 314 total aligned reads displayed.


CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180


cgacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.c                                                                                               >  2:3131442/1‑101 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                              <  2:5755796/101‑2 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                              <  2:1027413/101‑2 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                              >  2:225047/1‑100 (MQ=255)


    gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccggg                                                                                           >  2:1769810/1‑100 (MQ=255)


    gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccggg                                                                                           <  1:2929988/101‑2 (MQ=255)


       ggTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttg                                                                                        <  2:1196970/101‑5 (MQ=255)


       ggTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttg                                                                                        >  1:5348422/1‑97 (MQ=255)


          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggcg                                                                                     >  2:137144/1‑94 (MQ=255)


          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggag                                                                                     >  1:1079571/1‑94 (MQ=255)


          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggag                                                                                     <  1:4588054/101‑8 (MQ=255)


          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggag                                                                                     >  1:4347142/1‑94 (MQ=255)


          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggag                                                                                     >  2:5412069/1‑94 (MQ=255)


           cccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggaga                                                                                    <  1:4098488/100‑9 (MQ=255)


           aCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggaga                                                                                    >  1:842230/1‑93 (MQ=255)


            ccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGAt                                                                                    <  1:5044884/101‑1 (MQ=255)


             cAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATg                                                                                   >  1:2353719/1‑101 (MQ=255)


             cAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATg                                                                                   <  1:246835/101‑1 (MQ=255)


             cAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATg                                                                                   >  2:276715/1‑101 (MQ=255)


             cAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATg                                                                                   >  2:5373302/1‑101 (MQ=255)


             cAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATg                                                                                   >  2:4533974/1‑101 (MQ=255)


               gCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGcc                                                                                 >  2:4214735/1‑101 (MQ=255)


                 ggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCgc                                                                               >  2:1535470/1‑100 (MQ=255)


                 ggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGa                                                                               >  2:568841/1‑101 (MQ=255)


                 ggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGa                                                                               >  1:3577828/1‑101 (MQ=255)


                 ggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGa                                                                               >  2:3559049/1‑101 (MQ=255)


                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGAc                                                                              <  1:5064619/101‑1 (MQ=255)


                   ggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACa                                                                             <  1:4166677/101‑1 (MQ=255)


                   ggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACa                                                                             <  2:4826974/101‑1 (MQ=255)


                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATAct                                                                         >  1:1496112/1‑101 (MQ=255)


                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActct                                                                       <  2:1933969/101‑1 (MQ=255)


                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActct                                                                       <  1:397030/101‑1 (MQ=255)


                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActct                                                                       >  1:682980/1‑101 (MQ=255)


                           gaaaCAAAACTGCATAGATCAGCGTTGGGCTGCCCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAt                                                                     <  1:1168028/99‑1 (MQ=255)


                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAt                                                                     <  2:4000165/101‑1 (MQ=255)


                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAtt                                                                    <  2:4300468/101‑1 (MQ=255)


                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAtt                                                                    >  1:5228941/1‑101 (MQ=255)


                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAtt                                                                    >  2:3469870/1‑101 (MQ=255)


                             aaCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtc                                                                   >  2:1859898/1‑101 (MQ=255)


                               cAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtct.c                                                                >  2:3929326/1‑101 (MQ=255)


                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.cc                                                               >  1:4030078/1‑101 (MQ=255)


                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.cc                                                               >  1:328161/1‑101 (MQ=255)


                                  aaCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccct                                                             >  2:5491123/1‑99 (MQ=255)


                                  aaCTGCATAGAGCAGCGTTGGGCTGACCATGAACCAGCCGCGGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccct                                                             <  1:736930/101‑3 (MQ=255)


                                   aCTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctc                                                            <  1:4534246/101‑4 (MQ=255)


                                   aCTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctc                                                            <  2:2425068/101‑4 (MQ=255)


                                    cTGCATCGATCAGCGTTGGCCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctca                                                           <  1:962618/101‑5 (MQ=255)


                                    cTGCAGAGATCAGCGTGGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctca                                                           <  2:1143444/101‑5 (MQ=255)


                                      gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgc                                                          <  1:4892212/101‑1 (MQ=255)


                                        aTAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgc                                                        >  1:1052169/1‑101 (MQ=255)


                                          aGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgt                                                      >  1:1271063/1‑99 (MQ=255)


                                          aGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgt                                                      >  1:134345/1‑99 (MQ=255)


                                            aTCGGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtct                                                    <  1:4298028/101‑5 (MQ=255)


                                            aTCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtct                                                    >  2:2936236/1‑97 (MQ=255)


                                             tCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtctc                                                   <  2:4446448/101‑6 (MQ=255)


                                                gCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.gg                                               <  1:5532667/101‑2 (MQ=255)


                                                  gTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcg                                             >  2:2537268/1‑98 (MQ=255)


                                                   ttGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgc                                            <  2:1305557/101‑5 (MQ=255)


                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgcg                                           >  1:2137377/1‑96 (MQ=255)


                                                       tcTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  2:2650517/100‑1 (MQ=255)


                                                       gcggACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  1:1756816/98‑1 (MQ=255)


                                                       gcaacccATAAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  1:1670055/96‑1 (MQ=255)


                                                       gCTGACCATGACCCAGCCGCGGGCCGCCTCCCAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  1:276748/101‑1 (MQ=255)


                                                       gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  2:3817146/101‑1 (MQ=255)


                                                       gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  2:3309932/101‑1 (MQ=255)


                                                       gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         >  2:4690542/1‑101 (MQ=255)


                                                        cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTg                                        >  2:2629314/1‑101 (MQ=255)


                                                         tGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTgg                                       >  2:9286/1‑101 (MQ=255)


                                                          gACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTggg                                      <  2:5162056/101‑1 (MQ=255)


                                                            ccATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGcc                                    >  1:602241/1‑101 (MQ=255)


                                                            ccATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGAGGCCGACATACTCTATTGTCCCTCAGCGCGTCTCAGGCGCGTTTGGGcc                                    >  1:1892195/1‑101 (MQ=255)


                                                             cATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCa                                   >  2:2836393/1‑101 (MQ=255)


                                                              aTGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  <  1:2310607/101‑1 (MQ=255)


                                                              aTGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  >  1:2200774/1‑101 (MQ=255)


                                                              aTGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  >  2:2060328/1‑101 (MQ=255)


                                                               tGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaaa                                 >  1:5273107/1‑101 (MQ=255)


                                                                gacccAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAt                                <  1:545372/98‑1 (MQ=255)


                                                                     aGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                           <  2:3221365/101‑1 (MQ=255)


                                                                     aGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                           >  2:2332936/1‑101 (MQ=255)


                                                                        cGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCGCaa                        <  2:616800/101‑1 (MQ=255)


                                                                        cGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACaa                        >  2:562004/1‑101 (MQ=255)


                                                                         gCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                       >  1:3386295/1‑101 (MQ=255)


                                                                          cTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAgg                      <  1:1613476/101‑1 (MQ=255)


                                                                          cTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAgg                      <  2:4959448/101‑1 (MQ=255)


                                                                           tGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                     >  1:2056849/1‑101 (MQ=255)


                                                                            ggCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    >  1:2835543/1‑101 (MQ=255)


                                                                            ggCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    >  1:3546741/1‑101 (MQ=255)


                                                                             gccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGttt                   <  2:75141/101‑1 (MQ=255)


                                                                              ccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  >  2:1667424/1‑101 (MQ=255)


                                                                               cgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCg                 <  2:3627696/101‑1 (MQ=255)


                                                                               cgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCg                 <  1:1565690/101‑1 (MQ=255)


                                                                                  cTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa              >  2:3078220/1‑101 (MQ=255)


                                                                                  cTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa              >  1:1407706/1‑101 (MQ=255)


                                                                                   tCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag             <  1:2991528/101‑1 (MQ=255)


                                                                                        gcgcgcCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa        <  2:4346876/101‑1 (MQ=255)


                                                                                        gcgcgcCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa        <  1:2537410/101‑1 (MQ=255)


                                                                                         cgcgcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  1:3703609/96‑1 (MQ=255)


                                                                                           cgcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgg     <  1:4375936/98‑1 (MQ=255)


                                                                                           cgcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgg     >  1:257661/4‑101 (MQ=255)


                                                                                            gcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggg    >  2:2367802/3‑101 (MQ=255)


CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG