Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,833 +A 85.8% intergenic (‑599/‑303) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8331.→A85.8% 76.1 ‑0.4 ‑0.8176intergenic (‑599/‑303)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (68/83):  ref base (10/15):  total (78/98)
Fisher's exact test strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.64e-01

Only 100 of 188 total aligned reads displayed.

GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CCGCG.CGC.AGCG.TTCCGAACCGGCATCAATCTT.GG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAA  >  NC_014034/2057732‑2057911
                                                                                                          |                                                                                 
gTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.Cc.cg.cgc                                                                                    >  1:4727346/1‑101 (MQ=255)
gTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.Cc.cg.cgc                                                                                    >  2:5384777/1‑101 (MQ=255)
  ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.Cc.cg.cgccg                                                                                  <  1:5596783/101‑3 (MQ=255)
   cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGc                                                                                >  1:658477/1‑101 (MQ=255)
    tGTTTACCGCCCATTAGCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGca                                                                               <  1:825080/101‑2 (MQ=255)
     gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaa                                                                              >  2:4750152/1‑99 (MQ=255)
      tttTCCGCCCATTAGCCCTGCTTCGGCGCGTGGACTTCGGGGGGGCGGCGGGTCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaag                                                                             <  2:1890280/101‑4 (MQ=255)
      tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaag                                                                             >  2:2365727/1‑98 (MQ=255)
        tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcg                                                                           <  2:5039885/101‑6 (MQ=255)
        tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcg                                                                           >  1:2294712/1‑96 (MQ=255)
         aCCGCCCATTAACCCTGCTTAGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGccagcgt                                                                          >  2:913055/1‑95 (MQ=255)
           cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcgttt                                                                        <  2:3079999/101‑9 (MQ=255)
            gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcgtttc                                                                       <  1:4169772/101‑10 (MQ=255)
            gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcgtttc                                                                       <  2:3218194/101‑10 (MQ=255)
            gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcgtttc                                                                       <  2:4923648/101‑10 (MQ=255)
            gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcgtttc                                                                       <  1:1612047/101‑10 (MQ=255)
             cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcgtttcc                                                                      >  1:46918/1‑91 (MQ=255)
             cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcgtttcc                                                                      <  1:201301/101‑11 (MQ=255)
             cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcgtttcc                                                                      <  1:3819593/101‑11 (MQ=255)
             cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.CG.CGC.CGcaagcgtttcc                                                                      >  2:1555414/1‑91 (MQ=255)
                   aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCg                                                                    >  2:1573778/1‑101 (MQ=255)
                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGc                                                                  >  2:4443201/1‑101 (MQ=255)
                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGc                                                                  <  2:4288153/101‑1 (MQ=255)
                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGc                                                                  <  1:5738032/101‑1 (MQ=255)
                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGc                                                                  <  2:570466/101‑1 (MQ=255)
                      ccTGCTTCGGCGCGCGGACTCCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCa                                                                 >  2:1929864/1‑101 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                <  1:2531174/101‑1 (MQ=255)
                       cTGCTTCGCCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGCTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                >  2:4814538/1‑101 (MQ=255)
                        tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATc                                                               <  2:5235845/101‑1 (MQ=255)
                         gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCa                                                              <  1:5110190/101‑1 (MQ=255)
                         gCTTCGGCGCGCGGACTTCGGGGCGGAGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCa                                                              <  1:1491556/101‑1 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAA.C.CCGCGCCGCAAGCGTTTCCGAACCGGCAtaatt                                                            >  1:1156982/1‑97 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCatt                                                            <  2:2993693/101‑3 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCatt                                                            <  2:822817/101‑3 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCatt                                                            <  1:1029305/101‑3 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCatt                                                            >  1:1569643/1‑99 (MQ=255)
                            tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTc                                                           <  2:769837/101‑1 (MQ=255)
                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                          >  2:3745674/1‑101 (MQ=255)
                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                          <  2:3016835/101‑1 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                         >  1:602327/1‑101 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                         <  2:658420/101‑1 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                         >  2:2260781/1‑101 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                         <  1:3878468/101‑1 (MQ=255)
                               gcgcgcGGACGTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.g                                                       <  2:1103492/101‑1 (MQ=255)
                                cgcgcgGACTTCGGGGCAGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.gg                                                      <  1:2830365/101‑1 (MQ=255)
                                 gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GG.c                                                    <  1:2888731/101‑1 (MQ=255)
                                   gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GG.ccc                                                  >  1:2675772/1‑101 (MQ=255)
                                    cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GG.cccc                                                 >  1:1285168/1‑101 (MQ=255)
                                    ccgACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GG.cccc                                                 <  2:1024579/99‑1 (MQ=255)
                                     ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GG.ccccc                                                <  1:218588/101‑1 (MQ=255)
                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GG.cccccc                                               <  2:1788743/101‑2 (MQ=255)
                                       aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GG.ccccccg                                              <  1:4814819/101‑3 (MQ=255)
                                         ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GG.ccccccgcc                                            >  1:5209739/1‑97 (MQ=255)
                                         ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GG.ccccccgcc                                            <  1:1817323/101‑5 (MQ=255)
                                          tCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GG.ccccccgccg                                           <  2:5787656/101‑6 (MQ=255)
                                           cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCgg                                           >  1:779762/1‑101 (MQ=255)
                                           cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCgg                                           >  2:4593275/1‑101 (MQ=255)
                                            ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCggg                                          <  2:1408739/101‑1 (MQ=255)
                                            ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCggg                                          <  2:1655807/101‑1 (MQ=255)
                                             gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGa                                         <  2:5455272/101‑1 (MQ=255)
                                              ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGAc                                        <  1:2148911/101‑1 (MQ=255)
                                                cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCTCCGGGACaa                                      >  1:3448384/1‑101 (MQ=255)
                                                cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACaa                                      <  2:1309344/101‑1 (MQ=255)
                                                cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACaa                                      <  2:2927556/101‑1 (MQ=255)
                                                 ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTAGC.CCCCCGCCGGGACAAt                                     >  2:5500912/1‑101 (MQ=255)
                                                 ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAAt                                     <  2:2631554/101‑1 (MQ=255)
                                                   cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCa                                   >  1:2823821/1‑101 (MQ=255)
                                                    ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCAc                                  >  2:272798/1‑101 (MQ=255)
                                                    ggcggAGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCAc                                  >  2:4237504/1‑101 (MQ=255)
                                                      cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACgg                                >  2:5283798/1‑101 (MQ=255)
                                                      cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACgg                                >  2:717646/1‑101 (MQ=255)
                                                      cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACgg                                >  1:4605138/1‑101 (MQ=255)
                                                        tctctctTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGgcc                              <  2:3588296/98‑1 (MQ=255)
                                                        gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGgcc                              <  2:3291773/101‑1 (MQ=255)
                                                         ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGgccg                             >  2:1286767/1‑101 (MQ=255)
                                                           ctctTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGgccaga                           >  1:3588513/1‑98 (MQ=255)
                                                             ctTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGgccgcccc                         <  1:4221678/101‑3 (MQ=255)
                                                              ttGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCa                        <  1:4498252/101‑1 (MQ=255)
                                                              ttGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCa                        <  2:3465290/101‑1 (MQ=255)
                                                               tGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                       >  1:4763432/1‑101 (MQ=255)
                                                               tGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                       <  1:2337936/101‑1 (MQ=255)
                                                                    tCCTGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                  >  2:3699626/1‑101 (MQ=255)
                                                                       tGCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc               >  1:5375591/1‑101 (MQ=255)
                                                                        gCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc              <  2:3744794/101‑1 (MQ=255)
                                                                        gCGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc              <  1:1903106/101‑1 (MQ=255)
                                                                         cGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa             <  2:3511249/101‑1 (MQ=255)
                                                                         cGTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa             >  2:1178539/1‑101 (MQ=255)
                                                                          gTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt            >  1:1997454/1‑101 (MQ=255)
                                                                          gTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt            >  1:3038524/1‑101 (MQ=255)
                                                                          gTTTCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt            <  1:3765219/101‑1 (MQ=255)
                                                                            ttCCGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg          <  2:3910792/101‑1 (MQ=255)
                                                                             tCCGTATTGCCCAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcaagctg         <  2:4003679/101‑7 (MQ=255)
                                                                              ccGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg        >  1:67080/1‑101 (MQ=255)
                                                                               cGGATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGa       >  1:3907494/1‑101 (MQ=255)
                                                                                ggATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt      >  1:328837/1‑101 (MQ=255)
                                                                                 gATTGGCGAC.AACC.CCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc     <  1:5284088/101‑1 (MQ=255)
                                                                                 gATTGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc     <  1:913131/101‑1 (MQ=255)
                                                                                   ttGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa   >  2:1282623/1‑101 (MQ=255)
                                                                                    tGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa  <  2:2823660/101‑1 (MQ=255)
                                                                                    tGGCGAC.AA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTT.GGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa  >  2:4712998/1‑101 (MQ=255)
                                                                                                          |                                                                                 
GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CCGCG.CGC.AGCG.TTCCGAACCGGCATCAATCTT.GG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAA  >  NC_014034/2057732‑2057911

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG