BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,098,581	+C	2.3%	coding (48/1491 nt)	RCAP_rcc01939 →	polysaccharide biosynthesis protein family

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,098,581	1	.→C	2.3%	5.2 ‑3.3 ‑0.8	172	coding (48/1491 nt)	RCAP_rcc01939	polysaccharide biosynthesis protein family
Reads supporting (aligned to +/- strand): new base (4/0): ref base (73/95): total (77/95)
Fisher's exact test strand distribution p-value = 3.84e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.48e-01

Only 100 of 248 total aligned reads displayed.


TGCTTTCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCC.GCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCA  >  NC_014034/2098481‑2098678


 gCTTTCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.c                                                                                                     >  2:1442281/1‑101 (MQ=255)


  cTTTCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGG.c.c                                                                                                   >  2:1587957/1‑101 (MQ=255)


   tttCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGG.c.cc                                                                                                  >  2:2446880/1‑101 (MQ=255)


    ttCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGGCTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.c.ccc                                                                                                 <  1:5146059/101‑1 (MQ=255)


    ttCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.c.ccc                                                                                                 <  2:3801058/101‑1 (MQ=255)


     tCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCC.g                                                                                               <  2:2820083/101‑1 (MQ=255)


     tCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCC.g                                                                                               >  1:2513892/1‑101 (MQ=255)


      cGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCC.gg                                                                                              <  2:1114823/101‑2 (MQ=255)


      cGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCCCCCC.GG.C.GGCGGG.c.ccccg                                                                                               >  1:4476552/1‑99 (MQ=255)


        aaGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCC.ggct                                                                                            >  2:704197/1‑98 (MQ=255)


          ggCGGTCTGGCGCCTTCAGGGCCCCCGCACAGTATAGGGGACAGTGGCAGCCCATGCCCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCC.ggctcc                                                                                          <  2:1340070/101‑6 (MQ=255)


          ggCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCC.ggctcc                                                                                          <  2:3251390/101‑6 (MQ=255)


          ggCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCC.ggctcc                                                                                          >  1:2609753/1‑96 (MQ=255)


            cGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGG.C.CCCGGCTCCGc                                                                                         >  1:458611/1‑101 (MQ=255)


             ggTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCGCCACCCGCCGG.CG.C.GGGGGG.C.CGC.Gcctccccc                                                                                       >  1:1533943/1‑94 (MQ=255)


              gTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGc.cc                                                                                      >  2:1508361/1‑101 (MQ=255)


              gTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGc.cc                                                                                      <  1:1756342/101‑1 (MQ=255)


              gTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGG.C.CCCGGCTCCGc.cc                                                                                      >  2:4976045/1‑101 (MQ=255)


               tCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCCTGACCA.GCGAGTTG.CCCCCCCCCGCCCT.CG.C.TGCGGG.C.CCCGGCTCCCc.cca                                                                                     >  2:782096/1‑100 (MQ=255)


                  ggCGCCTTCACGGCACCCGCCCAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGc                                                                                  <  1:1026896/101‑1 (MQ=255)


                  ggCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCCGGc                                                                                  >  2:2623012/1‑101 (MQ=255)


                  ggCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCGCCGC.CCGGGc                                                                                  >  2:1170460/1‑101 (MQ=255)


                   gcgcCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGcc                                                                                 <  1:887845/101‑1 (MQ=255)


                   gcgcCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGG.C.CCCGGCCCCGC.CCGGGcc                                                                                 >  1:63481/1‑101 (MQ=255)


                    cgcCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCa                                                                                <  1:492193/101‑1 (MQ=255)


                     gcCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAg                                                                               <  1:958731/101‑1 (MQ=255)


                       cTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCCGGCCAGcc                                                                             >  1:3366053/1‑101 (MQ=255)


                       cTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGAACA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGTCC.CCC.GCTCCGC.CCCGGCCCGcc                                                                             >  2:3992481/1‑101 (MQ=255)


                        ttCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCCGccc                                                                            >  2:3774834/1‑100 (MQ=255)


                           ccGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCgg                                                                         <  2:494733/100‑1 (MQ=255)


                           aCGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGCCCA.GCGTTTTG.CCCCCCCCCCCCGC.GG.C.GGCGGC.C.CCCGGCTCCGC.CCGGGCCAGCCGCgg                                                                         <  1:2813960/101‑1 (MQ=255)


                             ggCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGAc                                                                       <  2:2513750/101‑1 (MQ=255)


                             ggCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGAc                                                                       <  1:508778/101‑1 (MQ=255)


                              gCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCCCCGC.CCGGGCCCGGCGCGGGcg                                                                      >  1:1609091/1‑101 (MQ=255)


                              gCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.CG.C.CGCCGC.C.CCC.CCCCCCCGCCGCGCCAACCGCGGAcg                                                                      >  2:4461434/1‑101 (MQ=255)


                                   cGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGGcggcgcg                                                                 >  1:2069227/1‑101 (MQ=255)


                                     cacaGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCCGGCCCGCCCCGGGcggggcggc                                                               >  1:4912629/1‑101 (MQ=255)


                                      acaGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCCC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGAcggggcggcg                                                              >  2:5518643/1‑101 (MQ=255)


                                           atgggggACGGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGAcggcgcggcgcggcg                                                         <  2:2943913/98‑1 (MQ=255)


                                           atgggggACGGTGCCACCCCATGCCCA.GCGATTCG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGAcggcgcggcgcggcg                                                         <  2:4927737/98‑1 (MQ=255)


                                            taGGGGACAGTGGCAGCCCATGACCA.GCGATTTGCCCCCCCCCCCCCCC.GG.C.GGCGGG.C.CCCGGCCCCGC.CCGGGCCCGCCGCCGGccgggcggggcgggg                                                         >  1:4157989/1‑99 (MQ=255)


                                             aGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCTCCCC.GG.C.GGCGGTCC.CCC.GCTCCGG.CCTCGCCACCCCCGGACCGCTCGCCgggggggg                                                       >  2:3382471/1‑100 (MQ=255)


                                             aGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCgcggcggg                                                       >  2:505783/1‑100 (MQ=255)


                                             aGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCCCCGC.GG.C.GGCGGTGC.CCC.GCGCCGC.CCGGGCCCGCCGCGGACCGCGCGGCgtgggggg                                                       >  1:4780330/1‑100 (MQ=255)


                                               gggACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCCC.CCGGGCCCCCCGCGGACGGCGCGGAGCGGGGGGGa                                                     >  1:2673081/1‑101 (MQ=255)


                                                  aCAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGTCC.CCC.GCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGCTCt                                                  >  2:1309683/1‑101 (MQ=255)


                                                  aCAGTGGC.GGCCATGTCCCGGCGATTTG.CCCCCCCCCGCCGC.GG.C.GGGGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCt                                                  <  2:631820/101‑1 (MQ=255)


                                                   cgggggCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCtt                                                 <  1:4503110/97‑1 (MQ=255)


                                                   cAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCCCCCC.GG.CGGGGGGC.C.CCC.GGTCCCC.CCCGGCCCGCCGCCGGCGGCCCCGCgcgggggggggccc                                                 >  1:1210463/1‑94 (MQ=255)


                                                    aGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCCCCCCGGG.G.GGGGGC.C.CCC.GCTCCCC.CCCGGGCCGCCCCGGGCGGCGCGGCgcgggggggggcttc                                                >  2:3145373/1‑93 (MQ=255)


                                                      tGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCCGCCGCGGACGGCGCGGCGCGGCGGCGGTCTTCGc                                              >  2:5334840/1‑101 (MQ=255)


                                                        gCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGGTCTTCGCCt                                            >  2:4478889/1‑101 (MQ=255)


                                                          aGCCCTTGCCCA.TCGTTTAG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCttt                                          <  2:1628214/101‑1 (MQ=255)


                                                          aGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCttt                                          >  2:4746396/1‑101 (MQ=255)


                                                           gCCCATGCCCG.GCGTTTTC.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTg                                         <  1:5441977/101‑1 (MQ=255)


                                                           gCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTg                                         >  2:1807874/1‑101 (MQ=255)


                                                             ccATGCCCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGAAGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTc                                       <  1:1004244/101‑1 (MQ=255)


                                                             ccATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTc                                       >  1:3912935/1‑101 (MQ=255)


                                                               aTGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGGcgc                                     >  1:809369/1‑101 (MQ=255)


                                                               aTGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCCTCCCCCTTGTcgc                                     >  2:2539793/1‑101 (MQ=255)


                                                                tGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcgcg                                    >  1:1644431/1‑101 (MQ=255)


                                                                   ccA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCgg                                 >  1:1895554/1‑101 (MQ=255)


                                                                    cA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGCTCCTCGCCTTTGTCGCGCggg                                >  2:479160/1‑101 (MQ=255)


                                                                    cA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCggg                                >  1:3760038/1‑101 (MQ=255)


                                                                     a.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGc                               >  1:1941985/1‑101 (MQ=255)


                                                                       gCGTTTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCg                              <  2:2330682/101‑1 (MQ=255)


                                                                        cGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGa                             <  1:5281222/101‑1 (MQ=255)


                                                                           tttG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCCGCCGCGGACGGCGCGGCGCGGCGGCGGTCTTCGCCTTTGTCGCCCCGGCGGCgc                          >  1:5323987/1‑101 (MQ=255)


                                                                           ttcg.CCCCCCCCCGCCGC.GG.C.GGCGGC.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgc                          <  2:4165798/98‑1 (MQ=255)


                                                                            ctctcccccccccGCCCT.GGCC.GGCGGT.CGCCG.GCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACg                           <  2:339320/97‑1 (MQ=255)


                                                                             tG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgcag                        <  2:1518713/101‑1 (MQ=255)


                                                                              gccccccccccGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgcag                        <  2:1020728/99‑1 (MQ=255)


                                                                              g.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgcagc                       >  1:5548333/1‑101 (MQ=255)


                                                                                cccccccccGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGAGgccgca                      >  1:2984638/1‑101 (MQ=255)


                                                                                cccccccccGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgcagca                      <  1:5472087/101‑1 (MQ=255)


                                                                                 ccccccccGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGcagcag                     <  2:259903/101‑1 (MQ=255)


                                                                                 ccccccccGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGcagcag                     <  1:3704701/101‑1 (MQ=255)


                                                                                 ccccccccGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGcagcag                     <  1:5223842/101‑1 (MQ=255)


                                                                                   ccccccGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCt                   <  1:4455662/101‑1 (MQ=255)


                                                                                   ccccccGCCGC.GG.C.GGCGGC.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCt                   <  2:4912348/101‑1 (MQ=255)


                                                                                     ccccGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCttt                 <  2:1644337/101‑1 (MQ=255)


                                                                                      cccGCCGC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTc                >  1:3552834/1‑101 (MQ=255)


                                                                                       ccgccgC.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCg               <  2:1995726/101‑1 (MQ=255)


                                                                                         gccgc.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGAc             >  2:1552595/1‑101 (MQ=255)


                                                                                         gccgc.GG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGAc             >  1:708444/1‑101 (MQ=255)


                                                                                           cgc.gG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCTGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCt           >  1:4243898/1‑101 (MQ=255)


                                                                                           cgc.gG.C.GGCGGT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCt           <  1:1459749/101‑1 (MQ=255)


                                                                                           ccc.gg.c.ggcggC.C.CCCGGCCCCGC.CGGGGCCAGCCGCGGACGGCCCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCt           <  2:5743333/99‑1 (MQ=255)


                                                                                             c.gg.c.ggcggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCttt         <  2:526821/101‑1 (MQ=255)


                                                                                             c.gg.c.ggcggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCttt         <  1:4348796/101‑1 (MQ=255)


                                                                                               gg.c.ggcggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTg        <  2:224508/101‑1 (MQ=255)


                                                                                               gg.c.ggcggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTg        <  2:3522312/101‑1 (MQ=255)


                                                                                               gg.c.ggcggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTg        <  2:4509428/101‑1 (MQ=255)


                                                                                                    ggcggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtca     >  2:4335042/1‑101 (MQ=255)


                                                                                                    ggcggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtca     >  1:2277784/1‑101 (MQ=255)


                                                                                                     gcggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcat    <  2:1895445/101‑1 (MQ=255)


                                                                                                     gcggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcat    <  1:1309771/101‑1 (MQ=255)


                                                                                                       ggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGGAGCTTTCCTCCTTTGtcatca  >  2:5153353/1‑101 (MQ=255)


                                                                                                       ggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatca  >  1:3085475/1‑101 (MQ=255)


                                                                                                       ggT.C.CCCGGCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatca  >  2:674161/1‑101 (MQ=255)


TGCTTTCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCA.GCGATTTG.CCCCCCCCCGCCGC.GG.C.GGCGGT.C.CCC.GCTCCGC.CCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCA  >  NC_014034/2098481‑2098678

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 21 ≤


ATCG

< 31 ≤


ATCG

< 41 ≤


ATCG