BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,177,270	+AG	100%	coding (107/990 nt)	rbsB ←	ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,177,270	1	.→A	100.0%	895.6	228	coding (107/990 nt)	rbsB	ribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand): new base (119/106): ref base (0/1): total (120/108)
*	NC_014034	2,177,270	2	.→G	100.0%	868.5	228	coding (107/990 nt)	rbsB	ribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand): new base (120/106): ref base (0/2): total (120/108)

Only 100 of 260 total aligned reads displayed.


CTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCCAACAGG  >  NC_014034/2177170‑2177360

||


 tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                                                                                    >  2:3961896/1‑100 (MQ=255)


 tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                                                                                    <  2:2667414/101‑2 (MQ=255)


 tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                                                                                    <  1:2326532/101‑2 (MQ=255)


  gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaag                                                                                                   >  1:5422304/1‑99 (MQ=255)


   cGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..agg                                                                                                >  2:4711242/1‑101 (MQ=255)


     tttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..agggg                                                                                              >  2:446336/1‑99 (MQ=255)


     tttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..agggg                                                                                              <  1:1298469/101‑3 (MQ=255)


     tttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..agggg                                                                                              >  1:1942339/1‑99 (MQ=255)


       tCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggtt                                                                                            <  1:153411/101‑5 (MQ=255)


       tCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggtt                                                                                            >  2:5150691/1‑97 (MQ=255)


         cacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttgg                                                                                          <  1:3962140/101‑7 (MQ=255)


           caTCGNNATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtg                                                                                        <  1:2106071/101‑2 (MQ=255)


           caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtg                                                                                        <  2:741878/101‑2 (MQ=255)


            aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgt                                                                                       <  2:2345341/101‑3 (MQ=255)


             tCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtc                                                                                      <  2:1409926/101‑4 (MQ=255)


             tCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtc                                                                                      <  1:2657367/101‑4 (MQ=255)


             tCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtc                                                                                      <  1:2084071/101‑4 (MQ=255)


              cGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcg                                                                                     >  2:5536411/1‑97 (MQ=255)


                ccATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggt                                                                                   <  1:163825/101‑7 (MQ=255)


                ccATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggt                                                                                   <  1:4687995/101‑7 (MQ=255)


                   tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtctt                                                                                <  2:3119959/101‑10 (MQ=255)


                    cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.g                                                                              <  1:446386/101‑1 (MQ=255)


                     gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.gg                                                                             >  2:4510478/1‑100 (MQ=255)


                      aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggt                                                                            <  2:3357001/101‑3 (MQ=255)


                      aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggt                                                                            <  1:596097/101‑3 (MQ=255)


                       tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggtg                                                                           >  1:1808231/1‑98 (MQ=255)


                       tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggtg                                                                           <  1:5510590/101‑4 (MQ=255)


                        cTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggtga                                                                          >  2:2598913/1‑97 (MQ=255)


                           gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggtgatca                                                                       <  1:3996932/101‑8 (MQ=255)


                           gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggtgatca                                                                       <  1:3150192/101‑8 (MQ=255)


                             ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggtgatcaga                                                                     >  1:1565486/1‑92 (MQ=255)


                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggtgatcagac                                                                    <  1:4329292/101‑11 (MQ=255)


                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggtgatcagac                                                                    >  1:4715385/1‑91 (MQ=255)


                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTt.ggtgatcagac                                                                    <  2:2128245/101‑11 (MQ=255)


                                cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGAtcagacag                                                                   <  1:3408913/101‑1 (MQ=255)


                                  tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGc                                                                 >  2:3259628/1‑101 (MQ=255)


                                   aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGcc                                                                >  2:1725561/1‑101 (MQ=255)


                                    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGccc                                                               >  2:41392/1‑101 (MQ=255)


                                     gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGcccc                                                              >  1:5564018/1‑101 (MQ=255)


                                      tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCg                                                             <  1:4574787/101‑1 (MQ=255)


                                       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCgg                                                            >  1:1092536/1‑101 (MQ=255)


                                       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCgg                                                            >  2:1983599/1‑101 (MQ=255)


                                       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCgg                                                            >  1:3825903/1‑101 (MQ=255)


                                        gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGt                                                           <  2:5725171/101‑1 (MQ=255)


                                          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                         >  2:5479939/3‑101 (MQ=255)


                                          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                         >  1:3489849/3‑101 (MQ=255)


                                           agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTc                                                        >  1:241213/2‑101 (MQ=255)


                                           agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTc                                                        >  1:1874264/2‑101 (MQ=255)


                                              aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                                     >  1:2021561/1‑101 (MQ=255)


                                               ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGcc                                                    <  1:3709626/101‑1 (MQ=255)


                                               ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGcc                                                    <  2:126432/101‑1 (MQ=255)


                                                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                   >  1:5436352/1‑101 (MQ=255)


                                                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                   >  1:5575080/1‑101 (MQ=255)


                                                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                   <  2:5563726/101‑1 (MQ=255)


                                                 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTGGACGAAAAAGGGGTTG..GTGGCGATCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                                 >  1:4947622/1‑101 (MQ=255)


                                                  gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aa                                                <  1:580533/101‑2 (MQ=255)


                                                  gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aa                                                <  1:4913284/101‑2 (MQ=255)


                                                   ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aag                                               <  1:801130/101‑3 (MQ=255)


                                                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                             <  1:2574211/101‑5 (MQ=255)


                                                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGCCTTGGTGATCAGACAGGCCCCGTTTTCTTCCG.aagcc                                             <  2:534336/101‑5 (MQ=255)


                                                        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                           >  1:1391040/1‑101 (MQ=255)


                                                        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                           >  2:3222941/1‑101 (MQ=255)


                                                         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                          >  2:1477988/1‑101 (MQ=255)


                                                         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                          <  2:773897/101‑1 (MQ=255)


                                                         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                          <  1:1195955/101‑1 (MQ=255)


                                                         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                          >  2:2498462/1‑101 (MQ=255)


                                                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                         <  2:2020097/101‑1 (MQ=255)


                                                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                         >  1:2613518/1‑101 (MQ=255)


                                                           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                        >  1:311254/1‑101 (MQ=255)


                                                           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                        <  2:3479941/101‑1 (MQ=255)


                                                             gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                      <  2:4837739/101‑1 (MQ=255)


                                                              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                     <  1:5334667/101‑1 (MQ=255)


                                                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                    <  2:917580/101‑1 (MQ=255)


                                                                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                   >  1:1784363/1‑101 (MQ=255)


                                                                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGACCCCGGTTTAGGCCGAAGCCGCCCCGGCCAg                                   >  1:649468/1‑101 (MQ=255)


                                                                  cTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                                 >  2:2213355/1‑101 (MQ=255)


                                                                   tttGCCGATGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                >  1:951457/1‑101 (MQ=255)


                                                                      gCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                             <  2:4254804/101‑1 (MQ=255)


                                                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                            <  2:4947341/101‑1 (MQ=255)


                                                                        cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                           <  2:3570228/101‑1 (MQ=255)


                                                                        cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                           >  1:459089/1‑101 (MQ=255)


                                                                          aaGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaacc                         >  2:1633183/1‑99 (MQ=255)


                                                                          aaGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                         >  1:4092636/1‑101 (MQ=255)


                                                                           aGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGTCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                        <  2:4016098/101‑1 (MQ=255)


                                                                            gCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                       >  2:4645383/1‑101 (MQ=255)


                                                                             ccccttccttCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                      >  2:3096143/3‑101 (MQ=255)


                                                                             cACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                      <  2:614810/101‑1 (MQ=255)


                                                                             cACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                      >  2:5252757/1‑101 (MQ=255)


                                                                              aCCTTCCTTCATCTTGACGAAAAAGGGGTTGCTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                     >  1:5159433/1‑101 (MQ=255)


                                                                                cttccttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                  <  2:1114196/101‑1 (MQ=255)


                                                                                cttccttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                  >  1:2440965/1‑101 (MQ=255)


                                                                                cttccttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                  <  1:894773/101‑1 (MQ=255)


                                                                                  tccttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGGTCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                >  2:2951746/1‑99 (MQ=255)


                                                                                  tccttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                >  1:5362427/1‑99 (MQ=255)


                                                                                    cttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag              <  2:3779468/101‑5 (MQ=255)


                                                                                      tcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag             <  2:1051796/101‑1 (MQ=255)


                                                                                       cATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCTGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg            >  1:4823276/1‑101 (MQ=255)


                                                                                       cATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg            >  2:2671445/1‑101 (MQ=255)


                                                                                       cATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg            <  1:3293122/101‑1 (MQ=255)


                                                                                        aTCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGc           >  2:3694177/1‑101 (MQ=255)

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CTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCCAACAGG  >  NC_014034/2177170‑2177360

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG