Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,285 +G 100% coding (92/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2851.→G100.0% 848.6 219coding (92/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (124/95):  ref base (0/0):  total (124/95)

Only 100 of 258 total aligned reads displayed.

CGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GG.GTTG..GT..CGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATC  >  NC_014034/2177190‑2177374
                                                                                                        |                                                                                              
cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.g                                                                                               <  1:446386/101‑1 (MQ=255)
cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.g                                                                                               <  1:641757/101‑1 (MQ=255)
cGATCTTGCCCGCATAGGTCGAGAGCACAAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.g                                                                                               >  2:3588443/1‑101 (MQ=255)
cGATCGTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.g                                                                                               <  1:933178/101‑1 (MQ=255)
 gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.gg                                                                                              >  2:4790346/1‑100 (MQ=255)
 gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.gg                                                                                              >  1:551096/1‑100 (MQ=255)
  aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggt                                                                                             >  1:1298072/1‑99 (MQ=255)
  aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggt                                                                                             <  2:3357001/101‑3 (MQ=255)
  aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCATTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggt                                                                                             <  1:4867682/101‑3 (MQ=255)
   tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggtg                                                                                            <  1:383104/101‑4 (MQ=255)
   tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggtg                                                                                            >  2:2668364/1‑98 (MQ=255)
   tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggtg                                                                                            >  1:1808231/1‑98 (MQ=255)
    cTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggtga                                                                                           >  2:2598913/1‑97 (MQ=255)
     ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggtgat                                                                                          >  2:1690490/1‑96 (MQ=255)
       gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggtgatca                                                                                        <  1:3150192/101‑8 (MQ=255)
        cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggtgatcag                                                                                       >  1:2601281/1‑93 (MQ=255)
          cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTt.ggtgatcagac                                                                                     >  1:4715385/1‑91 (MQ=255)
            cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAt.cagacag                                                                                   >  2:3921561/1‑101 (MQ=255)
             aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAgg                                                                                  >  1:4687733/1‑101 (MQ=255)
              tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGc                                                                                 >  2:3259628/1‑101 (MQ=255)
               aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGcc                                                                                >  1:952317/1‑101 (MQ=255)
                ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGccc                                                                               >  1:4020596/1‑101 (MQ=255)
                  tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCg                                                                             <  1:4574787/101‑1 (MQ=255)
                   cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCgg                                                                            >  2:660918/1‑101 (MQ=255)
                   cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCgg                                                                            >  1:1092536/1‑101 (MQ=255)
                    gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGt                                                                           <  2:5725171/101‑1 (MQ=255)
                      gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGttt                                                                         >  1:3489849/3‑101 (MQ=255)
                      gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGttt                                                                         >  2:5479939/3‑101 (MQ=255)
                       agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTc                                                                        >  1:1874264/2‑101 (MQ=255)
                       agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTc                                                                        >  1:241213/2‑101 (MQ=255)
                        gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                                       <  2:2384069/101‑1 (MQ=255)
                        gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                                       >  2:208500/1‑101 (MQ=255)
                          aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGc                                                                     >  1:2021561/1‑101 (MQ=255)
                           ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGcc                                                                    <  2:126432/101‑1 (MQ=255)
                            cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCg                                                                   >  2:4480119/1‑101 (MQ=255)
                             agtgccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                 >  1:542127/5‑101 (MQ=255)
                             aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTGGACG.AAAAAGGG.GTTG..GTGGCGATCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                 >  1:4947622/1‑101 (MQ=255)
                             aGCCCCATCTCGGTCGCCTTTGCCGAAGCAACTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACANGCCCCGGTTTCNGCNN.a                                                                 >  2:1409044/1‑101 (MQ=255)
                              gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aa                                                                <  2:1094928/101‑2 (MQ=255)
                               ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aag                                                               <  1:801130/101‑3 (MQ=255)
                                  cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagccg                                                            <  1:1542856/101‑6 (MQ=255)
                                   aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgc                                                            >  1:5489244/1‑101 (MQ=255)
                                     ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                          >  2:1477988/1‑101 (MQ=255)
                                     ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                          >  2:2498462/1‑101 (MQ=255)
                                     ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                          <  1:1195955/101‑1 (MQ=255)
                                          tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                     >  2:5169481/1‑101 (MQ=255)
                                           cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                    >  2:3976327/1‑101 (MQ=255)
                                            gCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGACCCCGGTTTAGGCCGAAGCCGCCCCGGCCAg                                                   >  1:649468/1‑101 (MQ=255)
                                             ccTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                                                  <  2:3218585/101‑1 (MQ=255)
                                              cTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGACCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                                                 >  1:5569997/1‑101 (MQ=255)
                                               tttGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                                <  2:3725676/101‑1 (MQ=255)
                                                 tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                              <  1:1707758/101‑1 (MQ=255)
                                                  gCCGAAGCACCTTCCTTCGTCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGGCAGCGCGGa                                             >  2:905712/1‑101 (MQ=255)
                                                   ccGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                            <  2:4947341/101‑1 (MQ=255)
                                                   ccGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                            <  2:5415603/101‑1 (MQ=255)
                                                   cagAAGCCACTTCGTTCATCGTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                            <  2:5539345/99‑1 (MQ=255)
                                                    cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                           <  2:3570228/101‑1 (MQ=255)
                                                     gAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                          <  1:4153524/101‑1 (MQ=255)
                                                     gAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                          >  1:4411750/1‑101 (MQ=255)
                                                      aaGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaacc                                         >  2:1633183/1‑99 (MQ=255)
                                                      aaGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                         <  2:4095034/101‑1 (MQ=255)
                                                      aaGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                         >  1:4824184/1‑101 (MQ=255)
                                                        gCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                       <  2:311221/101‑1 (MQ=255)
                                                        gCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                       >  1:193790/1‑101 (MQ=255)
                                                        gCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                       >  2:4645383/1‑101 (MQ=255)
                                                          aCCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                     >  1:3333928/1‑101 (MQ=255)
                                                            cttccttcATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                  >  1:2440965/1‑101 (MQ=255)
                                                            cttccttcATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                  <  1:5748221/101‑1 (MQ=255)
                                                              tccttcATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                >  1:5362427/1‑99 (MQ=255)
                                                               ccttcATCTTGACGAAAAA..GGCGTTGGTGT..CGGTCTT.GGGGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                               <  1:1633277/101‑4 (MQ=255)
                                                               ccttcATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                               <  2:5574788/101‑4 (MQ=255)
                                                                cttcATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                              >  1:5207972/1‑97 (MQ=255)
                                                                 ttcATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                             >  2:2140836/1‑96 (MQ=255)
                                                                 ttcATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                             <  2:2014193/101‑6 (MQ=255)
                                                                 ttcATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                             >  1:2096947/1‑96 (MQ=255)
                                                                 ttcATCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                             >  1:2073670/1‑96 (MQ=255)
                                                                    aTCTTGACG.AAAAAGGG.GTTGGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                          >  1:594642/1‑101 (MQ=255)
                                                                                   aggggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc              <  1:5279722/91‑1 (MQ=255)
                                                                                    ggggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa             >  1:2562219/10‑101 (MQ=255)
                                                                                    ggggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa             <  1:2213477/92‑1 (MQ=255)
                                                                                    ggggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa             <  2:4529569/92‑1 (MQ=255)
                                                                                       gttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt         >  2:2701208/7‑101 (MQ=255)
                                                                                        ttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt        <  1:3223141/96‑2 (MQ=255)
                                                                                        tggggtt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt        <  2:3064368/95‑2 (MQ=255)
                                                                                         tggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg       <  2:772782/97‑3 (MQ=255)
                                                                                          ggtgt..cggtcTTGGTGAT.TAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc      <  2:1909840/98‑4 (MQ=255)
                                                                                          ggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc      <  2:4188460/98‑4 (MQ=255)
                                                                                          ggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc      >  2:368603/4‑98 (MQ=255)
                                                                                          ggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc      <  2:3447890/98‑4 (MQ=255)
                                                                                           gtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg     <  1:3671023/99‑5 (MQ=255)
                                                                                           gtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg     <  1:3976571/99‑5 (MQ=255)
                                                                                           gtgt..cggtcTTGGTGAT.CAGACAGCCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg     >  2:1183173/3‑97 (MQ=255)
                                                                                            tgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc    <  1:375980/100‑6 (MQ=255)
                                                                                            tgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc    >  1:4118433/2‑96 (MQ=255)
                                                                                             gt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca    >  2:2992303/1‑101 (MQ=255)
                                                                                             gt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca    >  2:2844219/1‑101 (MQ=255)
                                                                                             gt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca    >  2:424126/1‑101 (MQ=255)
                                                                                              t..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcat   <  2:5316771/101‑1 (MQ=255)
                                                                                              t..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgagcat  >  1:2804273/1‑94 (MQ=255)
                                                                                                 cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc  >  1:1369569/1‑101 (MQ=255)
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CGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GG.GTTG..GT..CGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATC  >  NC_014034/2177190‑2177374

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG