Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,365 +T 100% coding (12/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3651.→T100.0% 979.2 249coding (12/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (123/125):  ref base (0/1):  total (123/126)

Only 100 of 283 total aligned reads displayed.

AGGGTTGGTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.T.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAATAT  >  NC_014034/2177270‑2177460
                                                                                                    |                                                                                                
ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t                                                                                                 >  2:1275394/8‑101 (MQ=255)
 gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.t                                                                                               <  1:4852411/95‑1 (MQ=255)
   tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAATGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttg                                                                                             >  1:4929047/5‑99 (MQ=255)
   tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttg                                                                                             >  2:2398772/5‑99 (MQ=255)
   tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttg                                                                                             >  1:4481657/5‑99 (MQ=255)
    ggtgtcggtcTTGGTGATTAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttgc                                                                                            <  2:1909840/98‑4 (MQ=255)
    ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttgc                                                                                            >  2:3952694/4‑98 (MQ=255)
    ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttgc                                                                                            >  1:5344879/4‑98 (MQ=255)
    ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttgc                                                                                            >  1:5228088/4‑98 (MQ=255)
     gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttgcg                                                                                           >  2:3049584/3‑97 (MQ=255)
     gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttgcg                                                                                           <  1:3976571/99‑5 (MQ=255)
      tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttgcgc                                                                                          <  1:375980/100‑6 (MQ=255)
      tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttgcgc                                                                                          >  1:4118433/2‑96 (MQ=255)
      tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttgcgc                                                                                          <  1:1725660/100‑6 (MQ=255)
      tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t.ttgcgc                                                                                          <  2:1483106/100‑6 (MQ=255)
       gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGca                                                                                          >  2:424126/1‑101 (MQ=255)
        tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGcat                                                                                         <  2:5316771/101‑1 (MQ=255)
        tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGcat                                                                                         >  2:367022/1‑101 (MQ=255)
        tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGcat                                                                                         >  2:550471/1‑101 (MQ=255)
         cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGcatc                                                                                        >  1:1369569/1‑101 (MQ=255)
            tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCatcatc                                                                                     >  2:2262382/11‑101 (MQ=255)
            tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCatcatc                                                                                     <  1:2978316/91‑1 (MQ=255)
              ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCAc                                                                                   <  1:3588660/93‑1 (MQ=255)
               tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACg                                                                                  >  1:4323030/8‑101 (MQ=255)
                ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACgg                                                                                 <  1:3259828/95‑1 (MQ=255)
                   gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGtct                                                                              <  2:4430529/98‑1 (MQ=255)
                   gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGtct                                                                              <  2:5416603/98‑1 (MQ=255)
                    atcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGtctc                                                                             >  1:5164820/3‑101 (MQ=255)
                      cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGtctctc                                                                           >  2:373145/1‑101 (MQ=255)
                       agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTctcc                                                                          >  2:271362/1‑101 (MQ=255)
                       agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTctcc                                                                          >  1:1547317/1‑101 (MQ=255)
                        gagagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTctcct                                                                         <  2:2628246/98‑1 (MQ=255)
                         acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTctcctc                                                                        >  1:1037473/1‑101 (MQ=255)
                          cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCtcctcc                                                                       <  1:4645659/101‑1 (MQ=255)
                           agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTccc                                                                      >  1:3210544/1‑101 (MQ=255)
                           agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTccc                                                                      <  1:2498604/101‑1 (MQ=255)
                           agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTccc                                                                      >  2:5433264/1‑101 (MQ=255)
                             gCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGt                                                                    <  2:2440831/101‑1 (MQ=255)
                             gCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGt                                                                    <  1:113793/101‑1 (MQ=255)
                               cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGttt                                                                  >  2:1809489/1‑101 (MQ=255)
                                ccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTc                                                                 >  2:285407/1‑101 (MQ=255)
                                 cGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                                                >  2:4502769/1‑101 (MQ=255)
                                  ggTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCAc                                                               >  1:3677825/1‑101 (MQ=255)
                                   gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                                              <  2:250440/101‑1 (MQ=255)
                                   gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                                              >  2:1564857/1‑101 (MQ=255)
                                    tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                                             >  2:2136670/1‑101 (MQ=255)
                                        ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                                         >  2:1193503/1‑101 (MQ=255)
                                        ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                                         >  1:4713726/1‑101 (MQ=255)
                                         gccgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGc                                                        <  1:684012/96‑1 (MQ=255)
                                           cgaaGCCGCCCCGGCCAGCGCGGAGAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                                                      <  1:2543797/98‑1 (MQ=255)
                                           cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                                                      >  1:3634032/4‑101 (MQ=255)
                                           cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                                                      <  1:5517419/98‑1 (MQ=255)
                                            gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                                     <  2:3209387/99‑1 (MQ=255)
                                            gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                                     >  2:857208/3‑101 (MQ=255)
                                             aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                                                    <  2:676588/100‑1 (MQ=255)
                                                 cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                                                >  1:1968580/1‑101 (MQ=255)
                                                  gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCGCCTCCCGTTTCACTGTCGGCCCCtcctcc                                               <  2:648713/101‑1 (MQ=255)
                                                        gCCAGCGGGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCGAGTT.TGCGCATCATCACGGTGTCTGGTGCCGTTTCACTGTCGGCCCCTCCTCTGGGGCt                                         >  1:2037117/1‑101 (MQ=255)
                                                          cAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg                                       >  2:5382026/1‑101 (MQ=255)
                                                          cAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg                                       <  2:4092389/101‑1 (MQ=255)
                                                           aGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCgg                                      >  2:338097/1‑101 (MQ=255)
                                                             cgcgGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc                                    <  2:5227801/101‑1 (MQ=255)
                                                             cgcgGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc                                    <  2:1093121/101‑1 (MQ=255)
                                                              gcgGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACa                                   >  1:1809664/1‑101 (MQ=255)
                                                              gcgGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACa                                   <  2:601727/101‑1 (MQ=255)
                                                                ggAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                                 >  1:3396036/1‑101 (MQ=255)
                                                                 gAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg                                <  1:2611870/101‑1 (MQ=255)
                                                                 gAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg                                <  2:1724569/101‑1 (MQ=255)
                                                                    aaCGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCt                             >  2:4343122/1‑101 (MQ=255)
                                                                     aCGCCAGGGCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg                            <  2:1882937/101‑1 (MQ=255)
                                                                       gccagggCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAc                          <  1:307588/96‑1 (MQ=255)
                                                                          agggCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGTGCTCGGACAAAGTCTGACCCg                       >  1:43894/3‑101 (MQ=255)
                                                                          agggCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCg                       >  2:3571069/3‑101 (MQ=255)
                                                                          agggCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCg                       >  2:2827825/3‑101 (MQ=255)
                                                                           gggCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGa                      <  1:3151502/100‑1 (MQ=255)
                                                                           gggCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGa                      >  2:5753917/2‑101 (MQ=255)
                                                                            ggCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg                     <  1:3009175/101‑1 (MQ=255)
                                                                            ggCAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg                     >  2:4851417/1‑101 (MQ=255)
                                                                              cAGAGGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc                   <  2:4715104/101‑1 (MQ=255)
                                                                               agagGCCCCCAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg                  >  2:5643662/1‑101 (MQ=255)
                                                                                 aggcccccAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGgc                <  1:1899441/97‑1 (MQ=255)
                                                                                 aggcccccAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGgc                <  1:618233/97‑1 (MQ=255)
                                                                                   gcccccAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGgcgc              >  2:3807227/3‑101 (MQ=255)
                                                                                   gcccccAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGgcgc              <  2:2353928/99‑1 (MQ=255)
                                                                                    cccccAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGAGcc             >  2:30029/2‑101 (MQ=255)
                                                                                     ccccAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGccc            >  2:3246917/1‑101 (MQ=255)
                                                                                      cccAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGcccc           >  2:2962468/1‑101 (MQ=255)
                                                                                      cccAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGcccc           >  1:5677302/1‑101 (MQ=255)
                                                                                       ccAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGTCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCg          <  1:4256433/101‑1 (MQ=255)
                                                                                        cAACAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCgg         >  2:3129137/1‑101 (MQ=255)
                                                                                         aaCAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGt        >  2:4654109/1‑101 (MQ=255)
                                                                                         aaCAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGt        >  2:2002694/1‑101 (MQ=255)
                                                                                          aCAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTc       <  1:2262508/101‑1 (MQ=255)
                                                                                          aCAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTc       <  1:2916607/101‑1 (MQ=255)
                                                                                          aCAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTc       <  1:1069501/101‑1 (MQ=255)
                                                                                           cAGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCa      <  1:976975/101‑1 (MQ=255)
                                                                                            aGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCaa     <  2:4928766/101‑1 (MQ=255)
                                                                                            aGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCaa     >  1:3549818/1‑101 (MQ=255)
                                                                                            aGGGCCAGTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCaa     <  2:2077983/101‑1 (MQ=255)
                                                                                               gCCAG.TATGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAatat  <  1:905788/101‑1 (MQ=255)
                                                                                                    |                                                                                                
AGGGTTGGTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.T.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAATAT  >  NC_014034/2177270‑2177460

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG