Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,240,244 +C 98.1% intergenic (+39/+139) RCAP_rcc02072 → / ← RCAP_rct00029 nonfunctional major facilitator superfamily protein/tRNA‑Gly

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,240,2441.→C98.1% 6.0 ‑1.5 ‑1.5259intergenic (+39/+139)RCAP_rcc02072/RCAP_rct00029nonfunctional major facilitator superfamily protein/tRNA‑Gly
Reads supporting (aligned to +/- strand):  new base (129/125):  ref base (1/4):  total (130/129)
Fisher's exact test strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.96e-02

Only 100 of 288 total aligned reads displayed.

GGCGTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.A.CCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGACGCAA  >  NC_014034/2240144‑2240339
                                                                                                       |                                                                                                
ggCGTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGa.a                                                                                                   >  2:5583121/1‑101 (MQ=255)
  cGTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAACCGTCCTT.GGGA.A.cc                                                                                                >  2:369651/1‑101 (MQ=255)
   gTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.A.ccc                                                                                               >  1:1002382/1‑100 (MQ=255)
   gTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.A.ccc                                                                                               <  1:5415901/101‑2 (MQ=255)
   gTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.A.ccc                                                                                               >  1:5348297/1‑100 (MQ=255)
    tGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.A.ccct                                                                                              >  2:1571420/1‑99 (MQ=255)
     gATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.A.ccctg                                                                                             <  1:4508290/101‑4 (MQ=255)
      aTACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.A.ccctgc                                                                                            >  2:4990348/1‑97 (MQ=255)
        aCTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTg.cgg                                                                                          >  1:5540249/1‑101 (MQ=255)
          tGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTg.cggcg                                                                                        <  2:5402212/101‑1 (MQ=255)
           gAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.cggcgg                                                                                       >  2:920708/1‑101 (MQ=255)
            gggACCGGCCGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGt                                                                                      <  2:1678201/100‑1 (MQ=38)
               aCAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTAtt                                                                                   >  1:863869/1‑101 (MQ=255)
                 aGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGc                                                                                 >  2:4329902/1‑101 (MQ=255)
                 aGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGGATTGc                                                                                 >  2:2492813/1‑101 (MQ=255)
                  ggcggcAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCGTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGcc                                                                                <  2:1908781/101‑1 (MQ=255)
                   gcggcAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCg                                                                               <  2:1560108/101‑1 (MQ=255)
                    cggcAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCgg                                                                              <  2:2699361/101‑1 (MQ=255)
                    cggcAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCgg                                                                              >  2:1803771/1‑101 (MQ=255)
                    cggcAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCgg                                                                              >  1:4250382/1‑101 (MQ=255)
                      gcAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCt                                                                            <  1:1571514/101‑1 (MQ=255)
                       cAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCtt                                                                           <  2:5358192/101‑1 (MQ=255)
                        aaGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTc                                                                          <  1:2486778/101‑1 (MQ=255)
                        aaGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTc                                                                          >  1:3489101/1‑101 (MQ=255)
                          gggCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTccc                                                                        <  2:2582011/101‑1 (MQ=255)
                           ggCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCg                                                                       >  1:4601349/1‑101 (MQ=255)
                           ggCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCg                                                                       <  2:485786/101‑1 (MQ=255)
                            gCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGa                                                                      >  1:2910028/1‑101 (MQ=255)
                              ttccttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGATTCCCGATc                                                                    >  2:5177007/1‑101 (MQ=255)
                               tccttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCt                                                                   >  2:5725667/1‑101 (MQ=255)
                                ccttcTGGGAATCGCCCGGCTTGCGCTTGATCTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTg                                                                  <  1:366871/101‑1 (MQ=255)
                                 cttcTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTgg                                                                 >  1:2089989/1‑101 (MQ=255)
                                    cTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGttt                                                              <  1:920784/101‑1 (MQ=255)
                                     tGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTg                                                             >  1:3600172/1‑101 (MQ=255)
                                     tGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTg                                                             <  1:1894373/101‑1 (MQ=255)
                                     tGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTg                                                             >  1:517853/1‑101 (MQ=255)
                                      gggAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGc                                                            >  1:1098997/1‑101 (MQ=255)
                                       ggAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGATC.CCTG.CGGCGGTTTTGCCGGCTTCCCGATCGGGTTTGCt                                                           <  2:5777800/101‑1 (MQ=38)
                                         aaTCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCa                                                         <  2:5512730/101‑1 (MQ=255)
                                         aaTCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGGTTGCTCa                                                         >  2:3153165/1‑101 (MQ=255)
                                           tCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAAc                                                       >  1:624449/1‑101 (MQ=255)
                                           tCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAAc                                                       <  1:733590/101‑1 (MQ=255)
                                            cGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACg                                                      >  1:5089262/1‑101 (MQ=255)
                                             gCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGt                                                     <  1:3153354/101‑1 (MQ=255)
                                             gCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGt                                                     <  1:3902904/101‑1 (MQ=255)
                                               ccGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGc                                                   <  1:4378461/101‑1 (MQ=255)
                                               ccGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGc                                                   <  1:1371400/101‑1 (MQ=255)
                                                 ggCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCtt                                                 >  2:1029250/1‑101 (MQ=255)
                                                 ggCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCtt                                                 >  2:41861/1‑101 (MQ=255)
                                                 ggCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCtt                                                 >  1:4344108/1‑101 (MQ=255)
                                                  gCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTc                                                <  1:3320451/101‑1 (MQ=255)
                                                  gCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTc                                                <  1:1802910/101‑1 (MQ=255)
                                                   cTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCa                                               <  2:1441741/101‑1 (MQ=255)
                                                    ttGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCCACGTGCTTCaa                                              >  2:2358550/1‑101 (MQ=255)
                                                    ttGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTTCTTCaa                                              <  2:1107355/101‑1 (MQ=255)
                                                    ttGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTTaa                                              >  2:2197501/1‑101 (MQ=255)
                                                      gcgcTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGa                                            >  1:5702912/1‑101 (MQ=255)
                                                       cgcTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGAc                                           >  2:4270547/1‑101 (MQ=255)
                                                        gcTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCGTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACa                                          <  1:4885308/101‑1 (MQ=255)
                                                         cTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACaa                                         >  2:3941907/1‑101 (MQ=255)
                                                         cTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTGCCCGATCTGGTTTGCTCAACGGGCTTCAAGCCaa                                         >  1:4967413/1‑101 (MQ=38)
                                                           tGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGc                                       >  1:4906919/1‑101 (MQ=255)
                                                            gAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGcc                                      >  2:3482850/1‑101 (MQ=255)
                                                            gAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGcc                                      >  1:4105810/1‑101 (MQ=255)
                                                              agtCGCGCTTGGGGAGGACCTGGGAAACGTCCGT.GGGA.ACCCTG.CGGCGGTATTGCGGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGcccc                                    <  2:3912540/99‑1 (MQ=255)
                                                                acgggcTTGGGAATAACCTCGGAAACGTGCTT.GGGACC.CCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAGGACAAGCCCCGt                                  <  2:1412833/97‑1 (MQ=255)
                                                                  gcgcTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAt                                <  1:2818604/101‑1 (MQ=255)
                                                                  gcgcTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAt                                <  1:456040/101‑1 (MQ=255)
                                                                  gcgcTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAt                                >  1:5464003/1‑101 (MQ=255)
                                                                   cgcTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAtg                               <  2:863793/101‑1 (MQ=255)
                                                                    cgctGGGTATGACCGCGGAAACGTCCTT.GGGA.A.CCCGCCGGCGGTATTGCCTGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAtgt                              <  2:753880/98‑1 (MQ=255)
                                                                     cTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGGACTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAtgtg                             >  1:1061800/1‑101 (MQ=255)
                                                                     cTTGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTAtgtg                             <  1:1660006/101‑1 (MQ=255)
                                                                       tGGGGATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCt                           <  2:1222445/101‑1 (MQ=255)
                                                                        ggggATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTc                          <  2:4776545/101‑1 (MQ=255)
                                                                         gggATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCg                         >  2:2086082/1‑101 (MQ=255)
                                                                         gggATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCg                         >  2:2978936/1‑101 (MQ=255)
                                                                           gATGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGaa                       <  1:1432493/101‑1 (MQ=255)
                                                                            aTGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTTCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTGTGTGCTCGAAt                      <  2:3837534/101‑1 (MQ=255)
                                                                            aTGACCTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAAt                      >  1:468026/1‑101 (MQ=255)
                                                                            aTGACCTCCGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAAt                      <  2:4399464/101‑1 (MQ=255)
                                                                                ccTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGc                  <  1:174983/101‑1 (MQ=255)
                                                                                 cTCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCa                 >  2:1269968/1‑101 (MQ=255)
                                                                                  tCGGAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCAc                <  2:4025142/101‑1 (MQ=255)
                                                                                    ggAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCg              >  1:3727956/1‑101 (MQ=255)
                                                                                     gAAACGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCgg             >  1:1614586/1‑101 (MQ=255)
                                                                                      aaaCGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGa            >  1:5589153/1‑101 (MQ=255)
                                                                                      aaaCGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGa            <  2:2752780/101‑1 (MQ=255)
                                                                                      aaaCGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGa            >  2:3392339/1‑101 (MQ=255)
                                                                                       aaggTCCTTGGGGA.C.CCTG.CGGTGGTATTGCGGGCTTCCGCATCTGTTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGaa           <  2:1605947/98‑1 (MQ=255)
                                                                                       aaCGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGaa           >  1:2764231/1‑101 (MQ=255)
                                                                                       aaCGTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGaa           <  1:1158658/101‑1 (MQ=255)
                                                                                          gTCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGCAggg        >  2:5649148/1‑101 (MQ=255)
                                                                                           tCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGa       <  1:1196251/101‑1 (MQ=255)
                                                                                           tCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGa       <  2:2265738/101‑1 (MQ=255)
                                                                                           tCCTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCCCGAATGTGCACCGGAAGGGa       <  2:373984/101‑1 (MQ=255)
                                                                                            ccTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGagatcgg      >  1:242034/1‑95 (MQ=255)
                                                                                            ccTT.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGAc      >  1:2694513/1‑101 (MQ=255)
                                                                                              tt.GGGA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGACga    >  2:4696940/1‑100 (MQ=255)
                                                                                                 gggA.ACCCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGACGCaa  <  1:2586261/101‑1 (MQ=255)
                                                                                                       |                                                                                                
GGCGTGATACTGAGGACAGGCGGCAAGGGCTTCCTTCTGGGAATCGCCCGGCTTGCGCTTGAACTCGCGCTTGGGGATGACCTCGGAAACGTCCTT.GGGA.A.CCTG.CGGCGGTATTGCCGGCTTCCCGATCTGGTTTGCTCAACGTGCTTCAAGACAAGCCCCGTATGTGCTCGAATGTGCACCGGAAGGGACGCAA  >  NC_014034/2240144‑2240339

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG