BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,249,300	+C	1.9%	intergenic (‑96/‑209)	parE ← / → RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,249,300	1	.→C	1.9%	9.5 0.0 ‑0.2	316	intergenic (‑96/‑209)	parE/RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand): new base (3/3): ref base (139/171): total (142/174)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.87e-01

Only 100 of 352 total aligned reads displayed.


ACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCC  >  NC_014034/2249201‑2249396


aCATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACt                                                                                                  <  2:1139335/101‑1 (MQ=255)


   tGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTccc                                                                                               >  1:5419302/1‑101 (MQ=255)


    ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTcccc                                                                                              <  2:2227978/101‑1 (MQ=255)


     gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTccccc                                                                                             <  1:1033908/101‑1 (MQ=255)


     gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTccccc                                                                                             <  2:584641/101‑1 (MQ=255)


     gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTccccc                                                                                             <  1:1553542/101‑1 (MQ=255)


      tGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTACCGGGACTCGCCCg                                                                                            >  2:3826060/1‑101 (MQ=255)


      tGCTCGCTTTCATTGGTGTTCTTAAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCg                                                                                            >  1:4655136/1‑101 (MQ=255)


        cTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCggg                                                                                          >  2:4393240/1‑101 (MQ=255)


         tCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGa                                                                                         <  1:821789/101‑1 (MQ=255)


         tCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGa                                                                                         <  2:2670571/101‑1 (MQ=255)


          cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGat                                                                                        >  1:3370071/1‑101 (MQ=255)


          cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGat                                                                                        >  2:3268013/1‑101 (MQ=255)


           gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGata                                                                                       >  1:75569/1‑101 (MQ=255)


           gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGata                                                                                       >  2:3463634/1‑101 (MQ=255)


            cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGatat                                                                                      >  2:5598961/1‑101 (MQ=255)


             tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                                     <  2:2729376/101‑1 (MQ=255)


             tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                                     <  2:3023410/101‑1 (MQ=255)


             tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGCCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                                     <  2:5054030/101‑1 (MQ=255)


               tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTg                                                                                   >  2:3172276/1‑101 (MQ=255)


               tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTg                                                                                   <  1:2778000/101‑1 (MQ=255)


                  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGTCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAgg                                                                                >  2:3510892/1‑101 (MQ=255)


                   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCGCCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGc                                                                               <  1:219927/101‑1 (MQ=255)


                   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGc                                                                               >  1:1001972/1‑101 (MQ=255)


                     gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCaa                                                                             <  1:3903039/101‑1 (MQ=255)


                     gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCaa                                                                             <  2:4157595/101‑1 (MQ=255)


                      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAg                                                                            >  2:4223218/1‑101 (MQ=255)


                        ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGAt                                                                          <  2:1549759/101‑1 (MQ=255)


                        ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGAt                                                                          >  2:1366134/1‑101 (MQ=255)


                         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                                         <  2:4962380/101‑1 (MQ=255)


                         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                                         <  1:1856827/101‑1 (MQ=255)


                            tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAg                                                                      <  2:1617584/101‑1 (MQ=255)


                            tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCACCCGGGCTCTTTCAGGCAAGATGAAg                                                                      >  2:4586881/1‑101 (MQ=255)


                             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAATGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgg                                                                     <  2:5163635/101‑1 (MQ=255)


                             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgg                                                                     <  2:3589158/101‑1 (MQ=255)


                             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgg                                                                     >  1:4559691/1‑101 (MQ=255)


                             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgg                                                                     <  2:2268175/101‑1 (MQ=255)


                              agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAggg                                                                    <  2:2370016/101‑1 (MQ=255)


                               gaTGCACAGATCCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgggg                                                                   <  1:5258103/101‑1 (MQ=255)


                               gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgggg                                                                   >  2:147620/1‑101 (MQ=255)


                               gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgggg                                                                   <  2:2146186/101‑1 (MQ=255)


                               gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgggg                                                                   >  1:420930/1‑101 (MQ=255)


                                 tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCAGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGGCgggggg                                                                 >  1:440113/1‑101 (MQ=39)


                                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCt                                                               <  1:1120856/101‑1 (MQ=255)


                                   cacaGAAACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCt                                                               <  1:959722/101‑1 (MQ=255)


                                    ccaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCtt                                                              <  2:277527/100‑1 (MQ=255)


                                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                                             <  1:2057049/101‑1 (MQ=255)


                                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                                             >  2:4825574/1‑101 (MQ=255)


                                     caGATACCGCAGCTTGGCCCCCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                                             <  2:2464731/101‑1 (MQ=38)


                                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                                            >  1:3071001/1‑101 (MQ=255)


                                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCAt                                                          <  2:4049277/101‑1 (MQ=255)


                                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCAt                                                          <  1:1388146/101‑1 (MQ=255)


                                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCAt                                                          <  2:1781233/101‑1 (MQ=255)


                                          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                                        >  2:2059805/1‑101 (MQ=255)


                                          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                                        <  2:4470016/101‑1 (MQ=255)


                                          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                                        <  1:5029491/101‑1 (MQ=255)


                                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTg                                                      <  1:190320/101‑1 (MQ=255)


                                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTg                                                      <  2:2963652/101‑1 (MQ=255)


                                             gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGc                                                     >  1:4278022/1‑101 (MQ=255)


                                              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGcc                                                    >  2:509718/1‑101 (MQ=255)


                                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCg                                                   <  2:4669100/101‑1 (MQ=255)


                                                gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGACATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGa                                                  >  1:5105964/1‑101 (MQ=255)


                                                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                                                 >  2:1073681/1‑101 (MQ=255)


                                                  ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaa                                                >  1:4238100/1‑101 (MQ=255)


                                                     ggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAata                                             <  1:4716094/101‑1 (MQ=255)


                                                      gCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAatat                                            >  1:635718/1‑101 (MQ=255)


                                                         cccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATccc                                         <  1:3172817/101‑1 (MQ=255)


                                                            ggggCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCggg                                      <  2:1109196/101‑1 (MQ=255)


                                                             gggCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgggg                                     >  2:826990/1‑101 (MQ=255)


                                                             gggCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgggg                                     <  2:5329162/101‑1 (MQ=255)


                                                                cAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                  <  1:5671797/101‑1 (MQ=255)


                                                                cAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                  <  1:3315069/101‑1 (MQ=255)


                                                                 aagaagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGTTATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCAggggggag                                 >  1:2369400/1‑99 (MQ=38)


                                                                 aagaagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgggggggg                                 >  1:2575173/1‑99 (MQ=255)


                                                                 aagaagGGAGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                 >  2:169533/1‑101 (MQ=255)


                                                                  agaagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                <  2:746087/101‑1 (MQ=255)


                                                                  agaagGGGGCTCGGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTCCTGAAAATATCCCGTGGGGTGa                                <  2:191458/101‑1 (MQ=37)


                                                                   gaagGGGGCTCGGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                               <  1:2945530/101‑1 (MQ=255)


                                                                    aagGGGGCTCTGCCCCC.GTCCCGTGCCCGCACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                              <  1:2256815/101‑1 (MQ=37)


                                                                       ggggCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                           <  2:4943000/101‑1 (MQ=255)


                                                                         ggCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                         <  1:3463851/101‑1 (MQ=255)


                                                                          gctctcccccc.GTCCCGTGCCGGGACCCCCCCGGGATTTTTGAGGAAAGAGGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa                        <  1:169553/94‑1 (MQ=37)


                                                                              tgccccc.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCa                    <  2:3915410/98‑1 (MQ=255)


                                                                              tgccccc.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCa                    <  1:3618486/98‑1 (MQ=255)


                                                                              tcccccc.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCACCCa                    <  2:4908152/98‑1 (MQ=255)


                                                                               gccccc.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                   <  1:1497574/99‑1 (MQ=255)


                                                                                   cacatccagtACCGGGACTCCCCCGGGATATTTGAGGCAAGCTGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgg                <  2:5704248/97‑1 (MQ=37)


                                                                                    c.gtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggg              >  2:475349/1‑101 (MQ=255)


                                                                                    c.gtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggg              >  2:251324/1‑101 (MQ=255)


                                                                                      gtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAggggg             <  1:251357/101‑1 (MQ=255)


                                                                                         ccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa          <  1:4941439/101‑1 (MQ=255)


                                                                                          cgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg         >  1:374616/1‑101 (MQ=255)


                                                                                           gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc        >  1:3575561/1‑101 (MQ=255)


                                                                                           gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc        >  2:4357325/1‑101 (MQ=255)


                                                                                           gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc        <  1:2671209/101‑1 (MQ=255)


                                                                                            tGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg       >  2:5475188/1‑101 (MQ=255)


                                                                                               cGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccc    >  1:1279654/1‑101 (MQ=255)


                                                                                                gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccc   >  1:3995097/1‑101 (MQ=255)


                                                                                                 ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccc  >  2:3739006/1‑101 (MQ=255)


                                                                                                 ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccc  <  2:5644335/101‑1 (MQ=255)


ACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCC  >  NC_014034/2249201‑2249396

Base quality scores:


ATCG

< 3 ≤


ATCG

< 19 ≤


ATCG

< 27 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG