Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,910 +G 100% coding (2665/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,9101.→G100.0% 866.1 245coding (2665/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (112/133):  ref base (0/0):  total (112/133)

Only 100 of 278 total aligned reads displayed.

AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCAT.GC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACCG  >  NC_014034/2304811‑2305005
                                                                                                       |                                                                                               
aaTCTTGCGCAAACCTCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCa                                                                                                  <  1:400176/101‑1 (MQ=255)
aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCa                                                                                                  >  2:1679730/1‑101 (MQ=255)
 aTCTTGCGCAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCA.g                                                                                                <  2:1516443/101‑1 (MQ=255)
 aTCTGGCGAAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCA.g                                                                                                <  1:2721627/101‑1 (MQ=255)
  tCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGGG.GAAGACCGCCGTCAGCCCCCGCA.gg                                                                                               >  2:4624213/1‑101 (MQ=255)
    ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCA.gggt                                                                                             <  1:3798024/101‑3 (MQ=255)
    ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCA.gggg                                                                                             >  1:5232665/1‑99 (MQ=255)
     tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCA.gggtc                                                                                            >  1:3016663/1‑98 (MQ=255)
     tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCA.gggtc                                                                                            >  2:3493391/1‑98 (MQ=255)
      gcgcAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                                           <  1:1337137/101‑5 (MQ=255)
       cgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGGCAGCTCCCGCA.gggcctg                                                                                          >  1:3325603/1‑96 (MQ=255)
        gcAAACCCCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgc                                                                                          <  1:2899182/101‑1 (MQ=255)
         cAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgcg                                                                                         >  2:3246294/1‑101 (MQ=255)
           aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGcc                                                                                       >  1:4616884/1‑101 (MQ=255)
             accgggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTc                                                                                     <  1:3923470/97‑1 (MQ=255)
               tCGGCGGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGc                                                                                   <  1:1571110/101‑1 (MQ=255)
                 ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                                 >  2:92961/1‑101 (MQ=255)
                 ggCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                                 <  1:5321264/101‑1 (MQ=255)
                     ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                                             >  2:1907623/1‑101 (MQ=255)
                      gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                                            >  1:2773021/1‑101 (MQ=255)
                      gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                                            <  1:1661474/101‑1 (MQ=255)
                        tCCGGCCGGGCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcg                                                                          <  2:1377169/101‑1 (MQ=255)
                         ccGGCCTGGCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcgc                                                                         <  1:5037421/101‑1 (MQ=255)
                          cGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGcc                                                                        >  1:4950144/1‑101 (MQ=255)
                           ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                                       <  1:2056345/101‑1 (MQ=255)
                            gCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                                      >  1:3376419/1‑101 (MQ=255)
                            gCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                                      <  2:762877/101‑1 (MQ=255)
                              cTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCtcgg                                                                    >  2:4410215/1‑98 (MQ=255)
                               tGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGt                                                                   >  1:1446985/1‑101 (MQ=255)
                                gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                                  >  1:1380302/1‑101 (MQ=255)
                                  cAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                                                <  2:4221167/101‑1 (MQ=255)
                                     ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGc                                                             <  1:4303129/101‑1 (MQ=255)
                                      cGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                                            <  1:4215245/101‑1 (MQ=255)
                                      cGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                                            >  1:3151735/1‑101 (MQ=255)
                                       ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                                           >  1:1301351/1‑101 (MQ=255)
                                       ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                                           <  2:5534323/101‑1 (MQ=255)
                                         ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                                         <  1:3681941/101‑1 (MQ=255)
                                         ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                                         >  2:1135828/1‑101 (MQ=255)
                                          cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGt                                                        >  2:1395519/1‑101 (MQ=255)
                                          cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGGCGCGGCCGCGt                                                        >  2:5220140/1‑101 (MQ=255)
                                          cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGCCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcgg                                                        >  1:3407257/1‑100 (MQ=255)
                                           tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtt                                                       <  2:4062365/101‑1 (MQ=255)
                                            gCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                                      >  1:1013549/1‑101 (MQ=255)
                                             ccGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                                     <  1:5159394/101‑1 (MQ=255)
                                             ccGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTAAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                                     >  1:4631187/1‑101 (MQ=255)
                                             ccGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAAACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                                     <  1:2423041/101‑1 (MQ=255)
                                              cGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                                    >  1:2922265/1‑101 (MQ=255)
                                                cAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                                  >  2:4288633/1‑101 (MQ=255)
                                                  aCGGCGGCGCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCTCGGCCGCGTTTTCCTCg                                                <  2:3080436/101‑1 (MQ=255)
                                                     gcggcgCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGacc                                             >  2:5424914/1‑99 (MQ=255)
                                                      cggcgCCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACa                                            <  1:3856483/101‑1 (MQ=255)
                                                       ggcgCCGGTGGC.TCCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                           <  1:1907732/101‑1 (MQ=255)
                                                        gcgcCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATc                                          <  1:5747159/101‑1 (MQ=25)
                                                         cgcCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                                         <  1:2588933/101‑1 (MQ=25)
                                                          gcCGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                                        <  2:1756745/101‑1 (MQ=25)
                                                           ccGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgcc                                       <  1:5514726/101‑1 (MQ=25)
                                                           ccGGTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgcc                                       >  1:1166506/1‑101 (MQ=25)
                                                             ggTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                     >  1:437334/1‑101 (MQ=25)
                                                             ggTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                     <  1:4824066/101‑1 (MQ=25)
                                                              gTGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                                    <  2:5488489/101‑1 (MQ=25)
                                                               tGGCG.CCTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                   >  2:16132/1‑101 (MQ=25)
                                                                 ggcgccTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                  <  2:4593268/97‑1 (MQ=25)
                                                                 ggcgccTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                  <  2:3726950/97‑1 (MQ=25)
                                                                 ggcgccTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                  <  2:3578520/97‑1 (MQ=25)
                                                                   cgccTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt                                <  1:5698960/99‑1 (MQ=25)
                                                                   cgccTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt                                <  1:622069/99‑1 (MQ=25)
                                                                   cgccTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt                                <  2:1487950/99‑1 (MQ=25)
                                                                   cgccTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt                                <  1:4526326/99‑1 (MQ=25)
                                                                    gccTGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg                               <  2:2070860/100‑1 (MQ=25)
                                                                       tGCAT.GCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg                            <  2:676197/101‑1 (MQ=25)
                                                                           atgcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt                         >  1:869312/6‑101 (MQ=21)
                                                                              cggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc                      >  2:2556781/3‑101 (MQ=21)
                                                                              cggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc                      <  1:979327/99‑1 (MQ=21)
                                                                               ggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAcc                     <  1:5618439/100‑1 (MQ=21)
                                                                               ggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAcc                     <  2:5154843/100‑1 (MQ=21)
                                                                               ggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGAGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAcc                     >  2:4986973/2‑101 (MQ=21)
                                                                                    accgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATc                <  1:4425709/91‑1 (MQ=21)
                                                                                     ccgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCa               <  1:958704/92‑1 (MQ=21)
                                                                                     ccgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGCCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCa               >  2:3001200/10‑101 (MQ=21)
                                                                                       gccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             >  1:1072828/8‑101 (MQ=21)
                                                                                       gccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             >  1:1842488/8‑101 (MQ=25)
                                                                                       gccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             <  1:2195471/94‑1 (MQ=21)
                                                                                       gccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             <  2:2367931/94‑1 (MQ=21)
                                                                                       gccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             >  2:1197532/8‑101 (MQ=21)
                                                                                       gccgtcagCTCCCGCAGGGCCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             <  2:894133/94‑1 (MQ=21)
                                                                                       cccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             <  2:2854714/94‑1 (MQ=21)
                                                                                          gtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTg          >  2:5734142/5‑101 (MQ=255)
                                                                                           tcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTgg         <  2:4453014/98‑1 (MQ=255)
                                                                                           tcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTgg         >  2:1268898/4‑101 (MQ=255)
                                                                                           tcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTgg         >  2:3373474/4‑101 (MQ=255)
                                                                                            cagCTCCCGCAGGGTCCGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGt        <  2:3827631/99‑1 (MQ=255)
                                                                                               cTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCa     >  2:2206063/1‑101 (MQ=255)
                                                                                               cTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCa     <  1:3493608/101‑1 (MQ=255)
                                                                                               cTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCa     <  2:1933985/101‑1 (MQ=255)
                                                                                                tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    <  2:4630911/101‑1 (MQ=255)
                                                                                                tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    >  2:3920321/1‑101 (MQ=255)
                                                                                                 cccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAcc   <  1:671895/101‑1 (MQ=255)
                                                                                                  ccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAccg  <  2:5100683/101‑1 (MQ=255)
                                                                                                  ccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAccg  <  1:2496340/101‑1 (MQ=255)
                                                                                                  ccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAccg  >  2:2192213/1‑101 (MQ=255)
                                                                                                       |                                                                                               
AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCAT.GC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACCG  >  NC_014034/2304811‑2305005

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG