Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,076 +C 14.4% intergenic (+124/‑118) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0760.→C14.4% 49.5 0.0 ‑0.3137intergenic (+124/‑118)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/5):  ref base (74/43):  total (85/52)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.97e-01

Only 100 of 151 total aligned reads displayed.

AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170
                                                                                                                                                                                                          
aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCa                                                                                                  <  2:1919512/101‑1 (MQ=18)
  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                                                >  1:101779/1‑99 (MQ=18)
  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                                                >  2:3927288/1‑99 (MQ=18)
   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                                               >  2:128492/1‑98 (MQ=18)
   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                                               >  2:2783474/1‑98 (MQ=18)
    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                                                                              >  1:2080257/1‑97 (MQ=18)
     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc                                                                                           >  2:596877/1‑101 (MQ=18)
      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.ccc                                                                                          >  1:2841308/1‑101 (MQ=18)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.ccc.cc                                                                                       <  2:2306639/101‑1 (MQ=17)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.ccc.cc                                                                                       >  2:224865/1‑101 (MQ=17)
         ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.ccc.ccc                                                                                      >  2:2990638/1‑100 (MQ=17)
            cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.ccc.ccctgc                                                                                   <  2:1085111/101‑5 (MQ=17)
              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGccc                                                                                  >  1:3401375/1‑101 (MQ=17)
              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGccc                                                                                  <  1:2517749/101‑1 (MQ=17)
                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCC.CCTGCCCcg                                                                                <  1:1558387/101‑1 (MQ=0)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgc                                                                               >  2:1503599/1‑100 (MQ=2)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgc                                                                               >  1:2232856/1‑100 (MQ=2)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgc                                                                               >  2:4053107/1‑100 (MQ=0)
                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCcgg                                                                               >  1:5353248/1‑101 (MQ=18)
                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAA.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCcgg                                                                               >  1:5262642/1‑101 (MQ=17)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                                              <  2:3021862/101‑3 (MQ=2)
                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCcggc                                                                              >  2:1738181/1‑101 (MQ=17)
                   tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgccg                                                                             >  2:254360/1‑101 (MQ=2)
                   tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgccg                                                                             >  2:496992/1‑101 (MQ=2)
                    atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgccgg                                                                            <  2:577947/101‑1 (MQ=2)
                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGG.AA.TGGC..GAAG.CCAGAGGGGGGC.CGCGACCC.CCTGCCCcggcgg                                                                            >  1:4038341/1‑101 (MQ=17)
                      ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgccggat                                                                          <  2:3238589/101‑3 (MQ=2)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTcc                                                                         <  2:5492465/101‑1 (MQ=17)
                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggc                                                                    >  2:1579395/1‑101 (MQ=17)
                              aGATGAAACCGGATTCCGTTCATCTTGCCTGAAATATTCCGGGGGGTG.AA.TGGA..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggcgg                                                                  <  1:2846712/101‑1 (MQ=9)
                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggcgg                                                                  >  2:4204943/1‑101 (MQ=18)
                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCt                                                             <  2:101759/101‑1 (MQ=17)
                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCt                                                             >  1:5411536/1‑101 (MQ=18)
                                    acccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGGGCCtt                                                            <  1:1954441/99‑1 (MQ=17)
                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                            >  1:5728138/1‑101 (MQ=18)
                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                            >  1:3949650/1‑101 (MQ=18)
                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGCCGGCGCCTTg                                                           >  2:1191765/1‑101 (MQ=17)
                                      ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGG.AA.TGGG..GAAG.CCCGAGGGGGGC.AGCG.CCCGCCTGCCCCGGCGGCCCGGCGCGGCGCCTGGa                                                          >  1:5192999/1‑101 (MQ=9)
                                        ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                                        >  2:3084474/1‑101 (MQ=17)
                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                                       >  2:342131/1‑101 (MQ=18)
                                          aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTgcgggg                                                      >  1:110544/1‑96 (MQ=17)
                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCAGCAC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                                     >  1:5365492/1‑101 (MQ=18)
                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                                     <  2:5283033/101‑1 (MQ=17)
                                             ccTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                                   <  2:5245935/101‑1 (MQ=17)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                                  >  1:138099/1‑101 (MQ=17)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                                  >  1:3962078/1‑101 (MQ=18)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 >  2:1404975/1‑101 (MQ=18)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 <  1:5669188/101‑1 (MQ=17)
                                               tttCATCTGGCCCGAAACATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 <  2:5191648/101‑1 (MQ=17)
                                                 tCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGT..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                                               >  2:390890/1‑100 (MQ=18)
                                                    tCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                                            >  2:786796/1‑101 (MQ=18)
                                                    tCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGAGGCGGCGCCTTGACGGGCGGGGGcgcg                                            >  1:4266567/1‑101 (MQ=18)
                                                     cTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCCCCTTGACGGGCGGGGGCGCgg                                           <  1:5455301/101‑1 (MQ=18)
                                                      ttGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCcgg                                          >  2:2503471/1‑101 (MQ=18)
                                                       tGCCCGAAATATCCCGGGGGGGG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgcacgc                                         >  2:5426171/1‑97 (MQ=18)
                                                          ccGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                      <  2:5639062/101‑1 (MQ=18)
                                                          ccGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                      <  2:5414592/101‑1 (MQ=18)
                                                            gAAATATGCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCAGCGG.TCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                    <  1:2674986/101‑1 (MQ=21)
                                                            gAAATATCCCGGGGGGTG.AA.TGGC..G.AA.GCCAGAGGGGGC.AGCGCCCC.CCCGCCCCCGCGGGCCGTGGCCGCGGCCTGACCGGGGGGGGCGCggggccttac                                   >  2:1157928/1‑94 (MQ=0)
                                                             aaaTATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                                   <  2:4504181/101‑1 (MQ=18)
                                                              aaTATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                                  >  1:215147/1‑101 (MQ=21)
                                                              aaTATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCACGGCCTTGACGGGCGGGGGCGCGGGCATTaaac                                  >  1:4992312/1‑99 (MQ=17)
                                                               atatCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg                                 >  1:4725475/1‑101 (MQ=21)
                                                               atatCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg                                 >  2:5222576/1‑101 (MQ=21)
                                                               atatCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGGCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTTACCg                                 >  1:4980211/1‑101 (MQ=18)
                                                               atatCCCGGGGGGGGAAA.GGGC..A.AG.CCAAAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg                                 <  2:1644352/101‑1 (MQ=25)
                                                                tatCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                <  1:4430595/101‑1 (MQ=21)
                                                                 atCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGGCGGGGGGGGGGGCGGGGATTAACCGaa                               >  1:3943106/1‑101 (MQ=12)
                                                                  tCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                              <  1:3685044/101‑1 (MQ=21)
                                                                   cccGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCTCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt                             <  1:4381020/101‑1 (MQ=21)
                                                                   cccGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGAATTAACCGAAAt                             <  1:3137359/101‑1 (MQ=21)
                                                                    ccGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt                            >  2:4893213/1‑101 (MQ=21)
                                                                    ccGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt                            >  2:3832046/1‑101 (MQ=21)
                                                                    ccGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt                            >  1:4447184/1‑101 (MQ=21)
                                                                      ggggggTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc                          <  2:4826904/101‑1 (MQ=25)
                                                                       gggggTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         >  2:636029/1‑101 (MQ=25)
                                                                       gggggTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         >  2:3301323/1‑101 (MQ=25)
                                                                        ggggTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa                        <  1:2698124/101‑1 (MQ=25)
                                                                          ggTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                      <  1:189655/101‑1 (MQ=25)
                                                                           gTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     <  1:4768207/101‑1 (MQ=25)
                                                                           gTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     <  1:341077/101‑1 (MQ=25)
                                                                                       gcgaagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc              <  1:780726/91‑1 (MQ=38)
                                                                                       gcgaagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc              <  2:2080140/91‑1 (MQ=38)
                                                                                        cgaagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCCTTAACCGAAATTGCAACAGATGCTGCg             >  1:4926267/10‑101 (MQ=37)
                                                                                         gaagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  1:3711867/9‑101 (MQ=38)
                                                                                         gaagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  2:1691036/9‑101 (MQ=38)
                                                                                         gaagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            <  1:2477353/93‑1 (MQ=38)
                                                                                          aagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           >  2:5749165/8‑101 (MQ=38)
                                                                                           agccagaGGGGGGCAGCGG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGTAATTGCAACAGATGCTGCGGTc          >  2:2420137/7‑101 (MQ=255)
                                                                                           agccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          >  2:1148991/7‑101 (MQ=38)
                                                                                            gccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGACGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATcc         >  1:4951043/6‑101 (MQ=38)
                                                                                             ccagaGGGGGGCAGCGG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        <  1:970876/97‑1 (MQ=255)
                                                                                             ccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  1:1453104/5‑101 (MQ=38)
                                                                                             ccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        <  1:1913167/97‑1 (MQ=38)
                                                                                              cagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       >  1:2953451/4‑101 (MQ=38)
                                                                                              cagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       >  2:3603209/4‑101 (MQ=38)
                                                                                              cagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       <  1:2075231/98‑1 (MQ=38)
                                                                                               agaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  2:1312977/3‑101 (MQ=39)
                                                                                                gaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGc     >  1:1051882/2‑101 (MQ=39)
                                                                                                gaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGCGGGGGCGCCCTTCCGGGCGGGGGCGCGGGGATTAACCGCAATTGGAACAGAAGCTCCGATCCCCGc     >  1:1130333/2‑101 (MQ=16)
                                                                                                                                                                                                          
AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG