Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,646,492 +C 2.2% intergenic (+450/+314) proS → / ← RCAP_rct00032 prolyl‑tRNA synthetase/tRNA‑Ala

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,646,4921.→C 2.2% 2.0 0.0 ‑0.2136intergenic (+450/+314)proS/RCAP_rct00032prolyl‑tRNA synthetase/tRNA‑Ala
Reads supporting (aligned to +/- strand):  new base (2/1):  ref base (98/35):  total (100/36)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.04e-01

Only 100 of 153 total aligned reads displayed.

GCAATCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCGGG  >  NC_014034/2646393‑2646591
                                                                                                      |                                                                                                    
gCAATCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCCCAA.CCATGAAGCACGGACC.TTG.c                                                                                                     >  1:522805/1‑101 (MQ=255)
 cAATCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCCTGAAGCCCGGACC.CTG.cc                                                                                                    >  1:3011465/1‑101 (MQ=255)
   aTCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.cccc                                                                                                  >  2:246001/1‑101 (MQ=255)
     cccGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCCGGCGCAAGCCGGGCGGCGGCGGCACCA.CCCTGAAGCCCCGACC.TTG.cccccc                                                                                                >  1:288856/1‑101 (MQ=38)
      ccGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.ccccccc                                                                                               >  1:3206275/1‑101 (MQ=255)
          cccGCGACACCCCCCCGCCGCCCGGCTTGCGCGGGGGACGCCCGGGCCCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCa                                                                                           <  1:4671333/100‑1 (MQ=38)
            cgcgACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCcga                                                                                         >  2:2510168/1‑101 (MQ=255)
            cgcgACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCcga                                                                                         >  2:1728697/1‑101 (MQ=255)
              ccacctccccccCCCCCCCGGCTTGCCGGCGGGGCCCCCGGGCCCAAGCCGGGGGGGGGGGGCCCAACCCATAAACCCCGGCCC.TT..CCCCCCCCCCAGAG.t                                                                                      <  2:1360/94‑1 (MQ=255)
               gACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.ccccccccccccaggg                                                                                      >  1:2469284/1‑95 (MQ=255)
                 cATCCCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCt                                                                                   <  1:4037586/101‑1 (MQ=255)
                  ctccccccGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGAAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTc                                                                                  <  1:302809/98‑1 (MQ=255)
                  ccccccccGCCCCCCGGGTTGGCGGGGGGGCGCCCGGGCGCAAGCCGGGCGGGGGCGGCCCAA.CCATGAAGCCCGGGCC.TTG.CCCCCCCCCCAGAG.TGCTc                                                                                  <  2:3011291/98‑1 (MQ=255)
                   cccccccGCGGCCCGGCGGGCGGGCGGGAGGCCCGGGATCCCCCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTcc                                                                                 <  1:141082/99‑1 (MQ=255)
                     cccccGCCCCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGCCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCg                                                                               <  1:4896211/101‑1 (MQ=255)
                       cccGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCCGAG.GGCTCCCGGc                                                                             >  2:4089947/1‑101 (MQ=255)
                         cgcgccccGGCTTGCCGCCGGGCCCCCCGGGGCAAAGCCGGGGGGGGGGGGCACAA.CCAAGAAGCCCGGACC.TTGCCCCCCCCCCCAGAG.TGCTCCCGGcc                                                                            <  2:3970944/96‑1 (MQ=255)
                           ccgccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCg                                                                         >  1:4706140/1‑101 (MQ=255)
                           ccgccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCCGCCCCg                                                                         >  1:2157785/1‑101 (MQ=255)
                           ccgccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GCCTCCCGGCCCCg                                                                         >  2:5028945/1‑101 (MQ=255)
                             gccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGGG.TGGCCCCGGCCCCggg                                                                       >  2:2660156/1‑100 (MQ=255)
                             gccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCggg                                                                       >  2:5361117/1‑100 (MQ=255)
                             gccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGa                                                                       >  2:5289060/1‑101 (MQ=255)
                              cccGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCCGAG.TGCTCCCGGCCCCGGAg                                                                      >  1:4007780/1‑101 (MQ=255)
                               ccGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTGCCCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAg                                                                      >  1:5788133/1‑101 (MQ=255)
                               ccGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGGgg                                                                     >  2:4228561/1‑101 (MQ=255)
                               ccGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAgg                                                                     >  2:2610158/1‑101 (MQ=255)
                               ccGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCCGCCCCGGAgg                                                                     >  2:4864484/1‑101 (MQ=255)
                                cGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAggg                                                                    >  1:2846145/1‑101 (MQ=255)
                                  gCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAggggg                                                                  >  1:4444581/1‑101 (MQ=255)
                                  gCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAggggg                                                                  >  1:5779302/1‑101 (MQ=255)
                                    ttGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCa                                                                >  1:4324100/1‑101 (MQ=255)
                                    ttGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.CGCTCCCGGCCCCGGAGGGGGCa                                                                >  1:880963/1‑101 (MQ=255)
                                     tGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCGCCCCCGGAGGGGGCAt                                                               >  1:696229/1‑101 (MQ=255)
                                      gcgcggGGCCCGCGCGGCCGCATGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TGG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGTGTGCATc                                                              <  2:1773625/101‑1 (MQ=255)
                                      gcgcgcGGGCCGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGGCC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATc                                                              <  1:2803810/101‑1 (MQ=255)
                                         cgcgGGCCGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG..CCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCa                                                          <  1:3713817/101‑1 (MQ=255)
                                         cgcgGGACGCCCTGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGc                                                           >  1:4391088/1‑101 (MQ=255)
                                          gcgGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCa                                                          >  2:2495329/1‑101 (MQ=255)
                                            gggACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTGCCCCCCCCCCCAGAG.TCCTCCCGGCCCCGGGGGGGGCATCGGCac                                                         >  1:2745047/1‑100 (MQ=255)
                                            gggACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAAc                                                        >  1:1189034/1‑101 (MQ=255)
                                             ggACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCGGCCCCGGAGGGGGCATCGGCAAcc                                                       >  2:114257/1‑101 (MQ=255)
                                               aCGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGGGACGCCCCCGCCCCCGG.GGGGGCACCCGCAAcccc                                                     >  1:1374459/1‑99 (MQ=255)
                                                cGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACcgcg                                                    >  1:4151942/1‑101 (MQ=255)
                                                 gCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCgcgc                                                   >  2:3339298/1‑101 (MQ=255)
                                                  cccGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGcc                                                  >  1:4547313/1‑101 (MQ=255)
                                                   tccgatctaaGCCGGGCGGCGGCGGCCCAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCa                                                 <  2:302220/93‑1 (MQ=255)
                                                   ccGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGccc                                                 >  2:5103133/1‑100 (MQ=255)
                                                   ccGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCa                                                 <  2:2845984/101‑1 (MQ=255)
                                                     gggCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCacg                                               >  1:5142627/1‑101 (MQ=255)
                                                         gcAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACgg                                           >  2:4414903/1‑101 (MQ=255)
                                                           aaGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGac                                         >  1:4107207/1‑101 (MQ=255)
                                                           aaGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAAAG.GGCTCCCGGCCCCGGGGGGGGCATCGGCAACCGCGCCACGACGGac                                         >  1:3653458/1‑101 (MQ=255)
                                                                gggCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGGG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCCCACCGCCGGACACcgg                                    >  2:3703112/1‑100 (MQ=255)
                                                                gggCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGCCACcgc                                    >  2:1395830/1‑101 (MQ=255)
                                                                gggCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACcgc                                    >  2:3381054/1‑101 (MQ=255)
                                                                gggCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACcgc                                    >  1:5420881/1‑101 (MQ=255)
                                                                 ggcggcggcggcACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACcgcg                                   >  1:1735231/1‑101 (MQ=255)
                                                                   cggcggcggcACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGGG.GGCCCCCGCCCCCGGGGGGGCCACCGGCAACCGCGCCCCAACGGACACcgcgct                                 >  1:1772700/1‑99 (MQ=255)
                                                                   cggcggcggcACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCCCGACGGACACCGCGAt                                 >  2:4800241/1‑101 (MQ=255)
                                                                    ggcggcggcACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCCCGACGGACACCGCGAtt                                >  2:1596842/1‑101 (MQ=255)
                                                                      cggcggcACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGa                              >  2:1501816/1‑101 (MQ=255)
                                                                      cggcggcACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCCCGCCCCGACGGACACCGCGATTGa                              >  1:5069098/1‑101 (MQ=255)
                                                                       ggcggcACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGAt                             >  1:3011014/1‑101 (MQ=255)
                                                                        gcggcACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCCGGATTGATc                            >  1:2424801/1‑101 (MQ=255)
                                                                        gcggcACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATc                            >  1:2019056/1‑101 (MQ=255)
                                                                         cggcACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCCCCCGGCCCCGGAGGGGGCATCGGCAACCCGCCCAAGCCGGAAACCCCGATTGATcc                           >  1:2352131/1‑101 (MQ=255)
                                                                             acaA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGccc                       >  1:3656480/1‑101 (MQ=255)
                                                                              caA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCGGcccc                      >  2:644649/1‑101 (MQ=255)
                                                                              caA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGcccc                      >  2:4210400/1‑101 (MQ=255)
                                                                               aa.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCg                     >  1:5654954/1‑101 (MQ=255)
                                                                                a.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCCCGACGGACACCGCGATTGATCCGCCCCgg                    >  1:3072028/1‑101 (MQ=255)
                                                                                a.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCgg                    >  1:215578/1‑101 (MQ=255)
                                                                                   cATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCTCCCCGGAt                  <  2:2157663/101‑1 (MQ=255)
                                                                                   cATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGAt                  >  2:1013924/1‑101 (MQ=255)
                                                                                   cATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGAt                  >  1:3552702/1‑101 (MQ=255)
                                                                                    aTGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATg                 <  1:2122307/101‑1 (MQ=255)
                                                                                       aaGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACa              <  2:2883558/101‑1 (MQ=255)
                                                                                        aGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAg             <  1:1013999/101‑1 (MQ=255)
                                                                                        aGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAg             <  1:4914880/101‑1 (MQ=255)
                                                                                        aGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAg             <  2:5779007/101‑1 (MQ=255)
                                                                                         gCCCGGACCTTTC.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAg             <  2:5254171/101‑1 (MQ=255)
                                                                                         gCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAgg            >  1:4536183/1‑101 (MQ=255)
                                                                                          cccGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGGGGGGGCATCGGCAACCCCGCCACGACGGAAACCGCGATTGATCCGCCCCGGATGACAgga           >  1:2359323/1‑101 (MQ=255)
                                                                                          cccGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAgga           <  2:4151692/101‑1 (MQ=255)
                                                                                           ccGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGGGGGGGCATCGGCAACCGCGCCCCGACGGACACCGCGATTGATCCGCCCCGGATGACAggac          >  1:3944905/1‑101 (MQ=255)
                                                                                           ccGGACC.TTC.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggac          <  2:3945563/101‑1 (MQ=255)
                                                                                            cGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacg         >  1:3754806/1‑101 (MQ=255)
                                                                                             ggACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacgg        <  1:2610307/101‑1 (MQ=255)
                                                                                             ggACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacgg        >  1:2458536/1‑101 (MQ=255)
                                                                                             ggACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacgg        >  1:4209533/1‑101 (MQ=255)
                                                                                               gcc.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacggac      <  1:1501909/100‑1 (MQ=255)
                                                                                               aCC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCTCGATTGATCCGCCCCGGACGACAgcccgtac      >  2:3731353/1‑94 (MQ=255)
                                                                                               aCC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacggac      <  2:4106958/101‑1 (MQ=255)
                                                                                               aCC.TTG..CCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGAcc     >  2:4380652/1‑101 (MQ=255)
                                                                                                cc.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGAcc     >  1:143685/1‑101 (MQ=255)
                                                                                                 c.TTC.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCg    <  2:522755/101‑1 (MQ=255)
                                                                                                   ttG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCgg   >  1:2900727/1‑101 (MQ=255)
                                                                                                   ttG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCgg   >  2:227264/1‑101 (MQ=255)
                                                                                                    tG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGGGATTGATCCGCCCCGGAGGACAGGCCGGACCggg  >  2:5662436/1‑101 (MQ=255)
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GCAATCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAA.CCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCGGG  >  NC_014034/2646393‑2646591

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG