Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,849	+G	100%	intergenic (‑181/+112)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,849	1	.→G	100.0%	79.9	26	intergenic (‑181/+112)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (0/26):  ref base (0/0):  total (0/26)

GTTCGGGCGGAATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943                                                                                                        |                                                                                                gTTCGGGCGGAATAGGAAACGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc                                                                                                  <  2:3720044/101‑1 (MQ=255)  ttCGGGCGAAAGAGCAACCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.g                                                                                                <  2:4009055/101‑1 (MQ=255)   tCGGGCGGAATAGGAAACGCGCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.gg                                                                                               <  1:5495149/101‑2 (MQ=255)    ggggCGGAATGGAAACCCCGCCCCGGGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggc                                                                                              <  1:1044154/100‑3 (MQ=255)      gggggAATGGGAACCGCGCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggcgg                                                                                            <  1:2061408/98‑5 (MQ=37)      ggggaaaTAGGAACCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggcgg                                                                                            <  1:855477/96‑5 (MQ=38)      ggCGGAATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggcgg                                                                                            >  2:2442891/1‑97 (MQ=255)        ggaaaTAGGAACCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggcgggc                                                                                          <  1:3548158/98‑7 (MQ=37)         ggAATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggcgggcg                                                                                         >  2:5303924/1‑94 (MQ=255)          gAATAGGAAACGCGCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggcgggcga                                                                                        <  2:3688408/101‑9 (MQ=255)           aaaaGGAACCGCGCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCgag                                                                                        <  2:4568645/98‑1 (MQ=37)            agaGGAAACGCGCCCCGCTTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCgaga                                                                                       <  2:1315581/99‑1 (MQ=37)             tAGGAAGCGCGACCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                      <  2:3790744/101‑1 (MQ=37)              aGGAACCGCGCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAg                                                                                     <  1:2605790/101‑1 (MQ=25)               ggAACCGCGCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAgc                                                                                    <  1:4153523/101‑1 (MQ=25)                 aaaCGCGCCCCGCGTTC.ACCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAgcgc                                                                                  <  2:5193131/101‑1 (MQ=25)                  accgccccccGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcc                                                                                 <  1:5604077/94‑1 (MQ=25)                   ccgcgCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                <  1:675307/100‑1 (MQ=25)                   acactaCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                <  2:193254/96‑1 (MQ=25)                   aCGCGCCCGGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                <  1:4221652/101‑1 (MQ=21)                   aCGCGCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                <  1:3168303/101‑1 (MQ=25)                    cgcgCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccg                                                                               <  2:1577389/101‑1 (MQ=25)                      cgcccccggTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCACCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                             <  1:5208470/93‑1 (MQ=21)                      cgccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                             <  2:1158162/98‑1 (MQ=25)                      cgccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                             <  1:1874333/98‑1 (MQ=25)                        ccccGCTTTG.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                          <  2:5618102/100‑1 (MQ=25)                        ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                          <  1:726977/100‑1 (MQ=25)                        ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                          <  2:1599325/100‑1 (MQ=25)                        ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                          <  1:5026240/100‑1 (MQ=25)                        ccccCCGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCccgcc.g                                                                           <  2:4207865/100‑1 (MQ=21)                         cccGTGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                          <  1:3869788/101‑1 (MQ=21)                          cccggTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                        <  2:928387/97‑3 (MQ=21)                          ccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                        <  1:3724269/101‑3 (MQ=25)                           cgcgTCC.CCCCGCCCGCAGCGCCCGGTGGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                       <  2:4176450/101‑4 (MQ=21)                             cgTTG.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                     <  1:2815315/101‑6 (MQ=25)                              ctcccccccGCCCGCAGCGCCGGGTGGCGGCATGCCCCCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                     <  1:1139008/96‑6 (MQ=18)                               tgt.CCCCGCCCGCGGCGCCCGGTTGCGGCATGGCCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                   <  2:5117143/99‑8 (MQ=21)                                                      ttGCGGCAGGACCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGC.CCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGcc                                              <  2:1423690/101‑1 (MQ=17)                                                             aTGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCC.GCGGGCGAGCAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGGCTTTGGGCCCTCcggg                                       >  2:617932/1‑101 (MQ=18)                                                                                            gcgcCCGG.CCCGGCGGGCGAGAGGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAgg        <  1:2443025/101‑1 (MQ=18)                                                                                              gcccgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg      >  1:453111/1‑101 (MQ=18)                                                                                              gcccgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg      >  1:5324893/1‑101 (MQ=18)                                                                                                 cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg   >  2:4163181/1‑101 (MQ=18)                                                                                                 cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg   >  2:614539/1‑101 (MQ=18)                                                                                                 cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg   >  2:73971/1‑101 (MQ=18)                                                                                                  gg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg  >  2:3932973/1‑101 (MQ=18)                                                                                                  gg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCCTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGCTgg  >  1:1287893/1‑101 (MQ=18)                                                                                                        |                                                                                                GTTCGGGCGGAATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG