Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,869	+G	100%	intergenic (‑201/+92)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,869	1	.→G	100.0%	70.3	27	intergenic (‑201/+92)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (25/2):  ref base (0/0):  total (25/2)

CGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCAT  >  NC_014034/2747770‑2747964                                                                                                        |                                                                                                cgcccccggTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCACCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                                                  <  1:5208470/93‑1 (MQ=21) cgccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                                                  <  1:1874333/98‑1 (MQ=25) cgccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                                                  <  2:1158162/98‑1 (MQ=25)   ccccGCTTTG.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                               <  2:5618102/100‑1 (MQ=25)   ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                               <  2:1599325/100‑1 (MQ=25)   ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                               <  1:726977/100‑1 (MQ=25)   ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                               <  1:5026240/100‑1 (MQ=25)   ccccCCGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCccgcc.g                                                                                                <  2:4207865/100‑1 (MQ=21)    cccGTGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                               <  1:3869788/101‑1 (MQ=21)     cccggTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                                             <  2:928387/97‑3 (MQ=21)     ccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                                             <  1:3724269/101‑3 (MQ=25)      cgcgTCC.CCCCGCCCGCAGCGCCCGGTGGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                                            <  2:4176450/101‑4 (MQ=21)        cgTTG.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                                          <  1:2815315/101‑6 (MQ=25)         ctcccccccGCCCGCAGCGCCGGGTGGCGGCATGCCCCCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                                          <  1:1139008/96‑6 (MQ=18)          tgt.CCCCGCCCGCGGCGCCCGGTTGCGGCATGGCCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                                        <  2:5117143/99‑8 (MQ=21)                                 ttGCGGCAGGACCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGC.CCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGcc                                                                   <  2:1423690/101‑1 (MQ=17)                                        aTGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCC.GCGGGCGAGCAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGGCTTTGGGCCCTCcggg                                                            >  2:617932/1‑101 (MQ=18)                                                                       gcgcCCGG.CCCGGCGGGCGAGAGGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAgg                             <  1:2443025/101‑1 (MQ=18)                                                                         gcccgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg                           >  1:5324893/1‑101 (MQ=18)                                                                         gcccgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg                           >  1:453111/1‑101 (MQ=18)                                                                            cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                        >  2:614539/1‑101 (MQ=18)                                                                            cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                        >  2:4163181/1‑101 (MQ=18)                                                                            cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                        >  2:73971/1‑101 (MQ=18)                                                                             gg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg                       >  2:3932973/1‑101 (MQ=18)                                                                             gg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCCTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGCTgg                       >  1:1287893/1‑101 (MQ=18)                                                                               gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc                      >  2:4724490/6‑101 (MQ=18)                                                                                 ccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCt                    >  2:4937026/4‑101 (MQ=21)                                                                                  cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt                   >  2:5063034/3‑101 (MQ=21)                                                                                   ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg                  >  1:4392729/2‑101 (MQ=21)                                                                                    gcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTgg                 >  1:5080120/1‑101 (MQ=21)                                                                                     cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc                >  1:4178537/1‑101 (MQ=21)                                                                                     cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc                >  2:396339/1‑101 (MQ=21)                                                                                      gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc               >  1:5253705/4‑101 (MQ=21)                                                                                      gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc               >  2:4788869/4‑101 (MQ=21)                                                                                        gcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCaa             >  2:2199393/2‑101 (MQ=21)                                                                                         cGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAg            >  2:250118/1‑101 (MQ=21)                                                                                         cGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAg            >  1:3461423/1‑101 (MQ=21)                                                                                          gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg           >  1:1244583/1‑101 (MQ=21)                                                                                            gaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTc         >  1:1510926/1‑101 (MQ=21)                                                                                            gaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTc         >  1:3696560/1‑101 (MQ=21)                                                                                             aaGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa        >  2:1113049/1‑101 (MQ=21)                                                                                             aaGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa        >  1:4410865/1‑101 (MQ=21)                                                                                              aGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAg       >  1:1887305/1‑101 (MQ=21)                                                                                                   ccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCAt  >  1:3168254/1‑101 (MQ=21)                                                                                                        |                                                                                                CGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCAT  >  NC_014034/2747770‑2747964 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG