Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,173,548 +C 100% intergenic (+583/‑99) tyrA → / → RCAP_rcc02983 prephenate dehydrogenase/extensin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,173,5481.→C100.0% 293.3 77intergenic (+583/‑99)tyrA/RCAP_rcc02983prephenate dehydrogenase/extensin family protein
Reads supporting (aligned to +/- strand):  new base (60/17):  ref base (0/0):  total (60/17)

CCCCGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.G.C.CGCCGCGCAAGCGC.A.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTG  >  NC_014034/3173449‑3173642
                                                                                                      |                                                                                                  
ccccGGCCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.g.c                                                                                                   <  1:3979123/101‑1 (MQ=255)
  ccGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.g.c.cc                                                                                                >  1:789370/1‑100 (MQ=255)
  ccGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.g.c.cc                                                                                                <  2:5339489/101‑2 (MQ=255)
   cGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCCCCCCCACCACTCCCGCAGCCCGGACGCCCCCGCCGGGCGGGGAGCTGTCGCGCCTTTT.CG.c.c.ccg                                                                                               >  1:3011950/1‑99 (MQ=255)
       cGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.cgccgc                                                                                            >  2:5750263/1‑101 (MQ=255)
           tCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCgcgcaa                                                                                        >  1:1667674/1‑101 (MQ=255)
           gagcccGCGCCGGTCACGCCCGCGCCCCCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCgcgcaa                                                                                        <  1:2513800/97‑1 (MQ=255)
                cgcgCCGGTCCCGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCgcgcaagcgc.a                                                                                  <  1:4931758/101‑1 (MQ=255)
                cgcgCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCgcgcaagcgc.a                                                                                  >  1:5767295/1‑101 (MQ=255)
                cgcgCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCgcgcaagcgc.a                                                                                  >  2:1576289/1‑101 (MQ=255)
                 gcgcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCgcgcaagcgc.a.a                                                                                >  2:2047838/1‑101 (MQ=255)
                 gcgcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCgcgcaagcgc.a.a                                                                                <  2:3660837/101‑1 (MQ=255)
                     cGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGCGCTGTCGCGCCTTTTCCG.C.C.CGCCgcgcaagcgc.a.acgcc                                                                            >  1:216528/1‑97 (MQ=255)
                      ggTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCgcgcaagcgc.a.acgccc                                                                           <  2:272991/101‑6 (MQ=255)
                        tCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGccccc                                                                         <  2:5753250/101‑1 (MQ=255)
                        tCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGccccc                                                                         <  2:5633027/101‑1 (MQ=255)
                         cACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCgcgcaagcgc.a.acgnnnnnn                                                                        >  1:1738201/1‑93 (MQ=255)
                           cgcccgccCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCccccgcc                                                                      <  2:5767000/101‑1 (MQ=255)
                           cccccaaccccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCccccgcc                                                                      <  2:3011776/94‑1 (MQ=255)
                             cccgcgcccgccACCACCACTCCCGCAGCCCGGAAGACGACCCCGGGGGGGGTGCTGTGGACACTCTT.CG.G.CGCGCCGCGCATGTCC.ACC.GCccccgcccc                                                                    >  2:966770/1‑101 (MQ=255)
                             cccgcccccgccCCCACCACTCCCGCAGCCCGGAAGCCGCCCCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCccccgcccc                                                                    >  1:5637667/1‑101 (MQ=255)
                               cgcccccgccccCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCgg                                                                  >  1:5236127/1‑101 (MQ=255)
                                  ccccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAg                                                               >  1:1516814/1‑101 (MQ=255)
                                  ccccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAg                                                               >  2:4357145/1‑101 (MQ=255)
                                   cccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgc                                                              >  2:2015859/1‑101 (MQ=255)
                                     cgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGCAA.A.GCCCCCGCCCCGGACgcgc                                                            >  2:123083/1‑101 (MQ=255)
                                       cccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgccc                                                          >  2:4628514/1‑101 (MQ=255)
                                        ccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgcccg                                                         >  2:4702765/1‑101 (MQ=255)
                                         cccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCCCCCCGGAAgcgcccgc                                                        >  2:1042883/1‑101 (MQ=255)
                                           caccacTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgcccgcgc                                                      >  2:2119390/1‑101 (MQ=255)
                                             ccacTCCCGCAGCCCGGAAGCCGCCGCCAGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGTCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgcccgcgccc                                                    >  1:1250079/1‑101 (MQ=255)
                                              cacTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGcgcccgcgcccg                                                   <  2:5637375/101‑1 (MQ=255)
                                              cacTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGcgcccgcgcccg                                                   >  1:2094842/1‑101 (MQ=255)
                                               acTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGa                                                  >  2:4972385/1‑101 (MQ=255)
                                               acTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGa                                                  >  2:2592545/1‑101 (MQ=255)
                                                 tCCCGCAGCCCGGAACCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                <  2:2595688/101‑1 (MQ=255)
                                                 ccccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                <  2:1521620/100‑1 (MQ=255)
                                                  cccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGc                                               >  1:4017866/1‑101 (MQ=255)
                                                   ccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGcc                                              <  2:789302/101‑1 (MQ=255)
                                                      cAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCt                                           >  2:159167/1‑101 (MQ=255)
                                                      cAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCt                                           >  2:425106/1‑101 (MQ=255)
                                                         cccGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCg                                        <  2:2094725/101‑1 (MQ=255)
                                                          ccGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCgg                                       >  2:4478968/1‑101 (MQ=255)
                                                            ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGcc                                     >  2:1302869/1‑101 (MQ=255)
                                                             gAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGccc                                    >  1:2385014/1‑101 (MQ=255)
                                                              aaGCCGCCGCCGGGCGGCGGGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCg                                   <  2:216495/101‑1 (MQ=255)
                                                               aGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCTGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGc                                  >  2:2948732/1‑101 (MQ=255)
                                                                  cgcggcggGGCGGCGTGCTGTCGCGCCTTTT.CGCC.C.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAgg                               <  1:574516/101‑1 (MQ=255)
                                                                  cgccgccgGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ATGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCCGCCCCCAgc                               >  1:4742942/1‑100 (MQ=255)
                                                                      gccgGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACCCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCCCAGGGCCg                           >  1:1033115/1‑101 (MQ=255)
                                                                       ccgGGCGGCGTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCgc                          >  2:1859621/1‑100 (MQ=255)
                                                                           gcggcgTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGccc                      >  1:4587791/1‑101 (MQ=255)
                                                                           gcggcgGGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGccc                      <  2:1667575/101‑1 (MQ=255)
                                                                             ggcgTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                    >  1:3229533/1‑101 (MQ=255)
                                                                             ggcgTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                    >  1:78674/1‑101 (MQ=255)
                                                                             ggcgTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                    >  1:352724/1‑101 (MQ=255)
                                                                              gcgTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCACCCCCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCCGc                   >  1:3830286/1‑101 (MQ=255)
                                                                               cgTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                  >  2:5220348/1‑101 (MQ=255)
                                                                               cgTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                  >  2:3032505/1‑101 (MQ=255)
                                                                                gTGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                 >  1:5552057/1‑101 (MQ=255)
                                                                                 tGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTg                >  1:5270567/1‑101 (MQ=255)
                                                                                 tGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTg                >  2:3112043/1‑101 (MQ=255)
                                                                                 tGCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTg                >  1:424581/1‑101 (MQ=255)
                                                                                  gCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc               <  1:5750558/101‑1 (MQ=255)
                                                                                  gCTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc               >  2:3745277/1‑101 (MQ=255)
                                                                                   cTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcg              >  2:1406775/1‑101 (MQ=255)
                                                                                   cTGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcg              >  1:575922/1‑101 (MQ=255)
                                                                                    tGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcgc             >  1:1662381/1‑101 (MQ=255)
                                                                                    tGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcgc             >  2:4179849/1‑101 (MQ=255)
                                                                                    tGTCGCGCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcgc             >  2:2974917/1‑101 (MQ=255)
                                                                                       cgcgCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGGGCCCGCGCCCGAAGCCCCTCCGCCCCGCAGGGCCGAGCCCGGCATGCGCggg          >  2:393715/1‑101 (MQ=255)
                                                                                       cgcgCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCggg          >  2:4001167/1‑101 (MQ=255)
                                                                                       cgcgCCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCggg          >  1:1524915/1‑101 (MQ=255)
                                                                                        ccgcCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggt         <  2:2384880/100‑1 (MQ=255)
                                                                                         cgcCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtc        >  2:4844402/1‑101 (MQ=255)
                                                                                         cgcCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtc        >  1:3408868/1‑101 (MQ=255)
                                                                                          gcCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGACGCCCCTCCGGCCCGCACGGCCGAGGCCCGGGTGcgcgcgccg       >  2:3304935/1‑96 (MQ=255)
                                                                                          gcCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcg       >  2:250564/1‑101 (MQ=255)
                                                                                          gcCTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcg       >  2:3667490/1‑101 (MQ=255)
                                                                                           ccTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcgg      >  1:4350418/1‑101 (MQ=255)
                                                                                            cTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcggt     >  1:3053066/1‑101 (MQ=255)
                                                                                            cTTTT.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGgggcggt     >  1:2664318/1‑101 (MQ=255)
                                                                                             tttt.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcggtc    <  2:5235840/101‑1 (MQ=255)
                                                                                              ttt.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt   >  1:3602654/1‑101 (MQ=255)
                                                                                               tt.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTg  >  1:389973/1‑101 (MQ=255)
                                                                                               tt.CG.GCC.CGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTg  >  2:5147215/1‑101 (MQ=255)
                                                                                                      |                                                                                                  
CCCCGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTT.CG.G.C.CGCCGCGCAAGCGC.A.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTG  >  NC_014034/3173449‑3173642

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG