BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,258,963	+C	100%	coding (778/1971 nt)	efeU →	ferrous iron permease EfeU

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,258,963	1	.→C	100.0%	96.9	243	coding (778/1971 nt)	efeU	ferrous iron permease EfeU
Reads supporting (aligned to +/- strand): new base (117/95): ref base (1/30): total (118/125)
Fisher's exact test strand distribution p-value = 6.44e-09
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.60e-04

Only 100 of 281 total aligned reads displayed.


CAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCTT  >  NC_014034/3258863‑3259058


  aTGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.cc                                                                                               <  1:1161956/101‑1 (MQ=255)


    gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccct                                                                                             >  1:1313726/1‑99 (MQ=255)


    gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccct                                                                                             <  1:1376490/101‑3 (MQ=255)


    gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccct                                                                                             <  2:1784793/101‑3 (MQ=255)


     ccTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccctg                                                                                            >  2:2035074/1‑98 (MQ=255)


       tCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccctgcc                                                                                          <  1:1541563/101‑6 (MQ=255)


        cGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCg                                                                                          >  2:5329927/1‑101 (MQ=255)


        cGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCg                                                                                          >  2:4671363/1‑101 (MQ=255)


        cGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCg                                                                                          >  2:3376649/1‑101 (MQ=255)


        cGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCg                                                                                          <  2:2459415/101‑1 (MQ=255)


         ggTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGc                                                                                         <  2:264859/101‑1 (MQ=255)


          gTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGct                                                                                        >  1:2345785/1‑101 (MQ=255)


            cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctct                                                                                      <  1:533202/101‑1 (MQ=255)


             gcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCtt                                                                                     >  2:5759926/1‑101 (MQ=255)


               gggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTcc                                                                                   >  2:1348508/1‑101 (MQ=255)


               gggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTcc                                                                                   <  1:5392019/101‑1 (MQ=255)


                ggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCt                                                                                  <  2:5365844/101‑1 (MQ=255)


                 gCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTg                                                                                 >  1:1879083/1‑101 (MQ=255)


                    ccGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgcgc                                                                              <  1:3622961/101‑1 (MQ=255)


                    ccGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgcgc                                                                              >  1:2510627/1‑101 (MQ=255)


                     cGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcc                                                                             <  1:4505802/101‑1 (MQ=255)


                     cGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcc                                                                             <  2:5169733/101‑1 (MQ=255)


                     cGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcc                                                                             <  2:3530916/101‑1 (MQ=255)


                      gTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGccc                                                                            <  1:2567943/101‑1 (MQ=255)


                       tGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcccc                                                                           >  2:5226605/1‑101 (MQ=255)


                       tGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcccc                                                                           <  1:5169829/101‑1 (MQ=255)


                        gCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcg                                                                          >  1:5751984/1‑101 (MQ=255)


                        gCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcg                                                                          <  2:1010316/101‑1 (MQ=255)


                           ttCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCgcgc                                                                       >  2:2572479/1‑101 (MQ=255)


                             cgacgcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     >  1:3301627/4‑101 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     <  2:4070161/101‑1 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     <  2:2121292/101‑1 (MQ=255)


                                 gcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCgcccgccc                                                                 >  1:3058904/1‑101 (MQ=255)


                                 gcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCgcccgccc                                                                 <  1:3898627/101‑1 (MQ=255)


                                 gcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCgcccgccc                                                                 >  1:4901392/1‑101 (MQ=255)


                                  ctctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCTTCCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccc                                                                <  1:1446518/101‑1 (MQ=255)


                                   tttttGGCATTCAAGACAGCCCGACCCGGGGCGAGGTCCCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGccccc                                                               <  2:2501004/99‑1 (MQ=255)


                                    ctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccccc                                                              <  1:5464463/101‑1 (MQ=255)


                                    ctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccccc                                                              >  1:1397139/1‑101 (MQ=255)


                                     tttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCt                                                             <  2:1878973/101‑1 (MQ=255)


                                         gCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCg                                                         >  2:4816796/1‑101 (MQ=255)


                                          cTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGc                                                        >  2:456365/1‑101 (MQ=255)


                                          cTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGc                                                        >  1:423988/1‑101 (MQ=255)


                                          cTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGc                                                        <  2:2613231/101‑1 (MQ=255)


                                             tCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaa                                                     >  2:1652041/1‑101 (MQ=255)


                                              cACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaaa                                                    <  2:1796023/101‑1 (MQ=255)


                                              cACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaaa                                                    >  2:4317649/1‑101 (MQ=255)


                                              cACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCCCCCCCCTTCCGCaaaa                                                    >  1:5631758/1‑101 (MQ=255)


                                               aCGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAg                                                   >  1:3397000/1‑101 (MQ=255)


                                               aCGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAg                                                   >  1:185420/1‑101 (MQ=255)


                                                cGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAgg                                                  >  2:954367/1‑101 (MQ=255)


                                                 gACAGCCCGACCCAGGGCGAGTTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGc                                                 <  1:217594/101‑1 (MQ=255)


                                                  aCAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGcc                                                <  1:3664372/101‑1 (MQ=255)


                                                     gCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGa                                             <  2:4641623/101‑1 (MQ=255)


                                                     gCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGa                                             <  2:3814390/101‑1 (MQ=255)


                                                     gCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGa                                             <  1:4022316/101‑1 (MQ=255)


                                                     gCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGATTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGa                                             <  1:4970203/101‑1 (MQ=255)


                                                      cccGGCCCGGGGGGGGGTCGCCCTCGGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGAt                                            <  1:318004/101‑1 (MQ=255)


                                                       ccGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATc                                           >  1:960459/1‑101 (MQ=255)


                                                        cgccccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATcc                                          <  1:2624880/98‑1 (MQ=255)


                                                        cGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATcc                                          <  2:5663832/101‑1 (MQ=255)


                                                        cGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATcc                                          <  1:2255752/101‑1 (MQ=255)


                                                        cGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATcc                                          >  1:463478/1‑101 (MQ=255)


                                                        cGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATcc                                          <  1:410127/101‑1 (MQ=255)


                                                        cGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATcc                                          >  1:2966205/1‑101 (MQ=255)


                                                         gACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCTCAAAAGGCCTGATCCg                                         >  1:4357426/1‑101 (MQ=255)


                                                          aCCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGa                                        <  2:792935/101‑1 (MQ=255)


                                                             cAGGGCGAGGTCGCCCTCCGGGCCGCGTTCCTGCCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGa                                     <  2:3972876/101‑1 (MQ=255)


                                                              aGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAc                                    >  1:192108/1‑101 (MQ=255)


                                                               gggCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAcc                                   <  1:750623/101‑1 (MQ=255)


                                                                ggCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAccc                                  >  1:2049491/1‑101 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               <  2:4155642/101‑1 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               >  2:1559163/1‑101 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               >  2:3959547/1‑101 (MQ=255)


                                                                     ggTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                             <  1:4752554/101‑1 (MQ=255)


                                                                      gTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCg                            <  1:3434550/101‑1 (MQ=255)


                                                                         gCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCTTCCCGcac                         >  1:890895/1‑101 (MQ=255)


                                                                         gCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGcac                         <  2:3622939/101‑1 (MQ=255)


                                                                         gCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGcac                         >  2:1061331/1‑101 (MQ=255)


                                                                           ccgctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACaa                       <  2:217825/98‑1 (MQ=255)


                                                                           ccTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACaa                       <  1:1219471/101‑1 (MQ=255)


                                                                           ccTCCGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACaa                       <  1:5557765/101‑1 (MQ=255)


                                                                              gggggCGGCGTTCTTCGCCTTGTC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcg                    <  1:2937477/99‑1 (MQ=255)


                                                                              ctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcg                    >  1:1076423/1‑101 (MQ=255)


                                                                               tGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcgc                   >  1:3744092/1‑101 (MQ=255)


                                                                               tGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcgc                   <  2:127297/101‑1 (MQ=255)


                                                                                gggCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGcc                  >  2:2826541/1‑101 (MQ=255)


                                                                                 ggCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCg                 <  2:3743864/101‑1 (MQ=255)


                                                                                 ggCCGCGTCCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCg                 <  2:5050180/101‑1 (MQ=255)


                                                                                    cgctTTCCTGCCCGCG.ACCCTGCCGCTCTTCCTCCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGTCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTcc              <  1:1102542/101‑1 (MQ=255)


                                                                                    cgcgTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTcc              <  2:2363708/101‑1 (MQ=255)


                                                                                    cgcgTTCCTGCCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTcc              <  2:1397051/101‑1 (MQ=255)


                                                                                     gcgTTCCGGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCg             <  2:5463414/101‑1 (MQ=255)


                                                                                      ctttCCGGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCTTTCCGCACAAGCGCCGTCCgg            <  2:890819/99‑1 (MQ=255)


                                                                                        ttCCTGGCCGTGCC.CCTGCCGCTCTTCCTGTGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc          <  1:2690126/101‑1 (MQ=255)


                                                                                        ttCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc          >  1:1633931/1‑101 (MQ=255)


                                                                                        tcccTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc          <  2:5589054/99‑1 (MQ=255)


                                                                                           cTGGCCGTG.ACCCTGCCGCTCTTCCTGAGCCCCGCGCCCGCACCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGAGCCGTCCGGgcgac       >  2:3869520/1‑99 (MQ=255)


                                                                                             ggCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctc     >  1:5794861/1‑101 (MQ=255)


                                                                                               ccGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctcct   <  2:4750972/101‑1 (MQ=255)


CAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCTT  >  NC_014034/3258863‑3259058

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG