BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,412,109	+G	2.1%	intergenic (‑76/‑131)	talC ← / → priA	transaldolase/primosomal protein N`

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,412,109	1	.→G	2.1%	5.0 ‑4.4 ‑1.3	187	intergenic (‑76/‑131)	talC/priA	transaldolase/primosomal protein N`
Reads supporting (aligned to +/- strand): new base (4/0): ref base (60/123): total (64/123)
Fisher's exact test strand distribution p-value = 1.29e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.54e-02

Only 100 of 251 total aligned reads displayed.


CGGCGGTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAATACGCCC  >  NC_014034/3412009‑3412208


cggcggTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCaa                                                                                                        >  2:3348692/1‑101 (MQ=255)


 ggcggTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.g                                                                                                      >  1:1029453/1‑101 (MQ=255)


 ggcggTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.g                                                                                                      >  1:251202/1‑101 (MQ=255)


 ggcggTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.g                                                                                                      <  2:118156/101‑1 (MQ=255)


 ggcggTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.g                                                                                                      >  2:1649525/1‑101 (MQ=255)


 ggcggTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.g                                                                                                      >  2:2127623/1‑101 (MQ=255)


   cggTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.ggg                                                                                                    >  2:256748/1‑101 (MQ=255)


   cggTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gga                                                                                                    >  2:4873606/1‑100 (MQ=255)


    ggTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gggg                                                                                                   >  2:4377264/1‑101 (MQ=255)


    ggTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gggg                                                                                                   <  2:5009311/101‑1 (MQ=255)


      tATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gggggg                                                                                                 <  1:3604472/101‑1 (MQ=255)


       aTCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.ggggggg                                                                                                <  2:4198980/101‑1 (MQ=255)


       aTCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.ggggggg                                                                                                <  1:1399249/101‑1 (MQ=255)


       aTCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.ggggggg                                                                                                >  1:1190060/1‑101 (MQ=255)


       aTCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.ggggggg                                                                                                >  1:4327372/1‑101 (MQ=255)


         cgacgaAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCt                                                                                              <  1:281501/101‑1 (MQ=255)


          gacgaAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCtt                                                                                             <  1:129444/101‑1 (MQ=255)


          gacgaAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCtt                                                                                             >  1:4543090/1‑101 (MQ=255)


          gacgaAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGACGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGtt                                                                                             >  1:1471133/1‑101 (MQ=255)


            cgaAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gggggggggggg                                                                                           >  1:813072/1‑96 (MQ=255)


             gaagaaTTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTg.cc                                                                                         <  2:2637371/101‑1 (MQ=255)


             gaagaaTTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTg.cc                                                                                         <  2:2490604/101‑1 (MQ=255)


              aagaaTTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gggggggggggggg                                                                                         >  2:1311303/1‑94 (MQ=255)


              aagaaTTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gggggggggggggg                                                                                         >  1:4534539/1‑94 (MQ=255)


               agaaTTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTg.ccgc                                                                                       >  1:2884526/1‑101 (MQ=255)


                 aaTTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGCGCGGAATCGGGCGCAA.gggcggggggagaggcg                                                                                      >  1:4977445/1‑91 (MQ=255)


                 aaTTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGgcc                                                                                     >  2:3491844/1‑101 (MQ=255)


                 aaTTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGccc                                                                                     <  2:1986119/101‑1 (MQ=255)


                  aTTTGACGAAGGTGCTCCGGGCCTCTGTCTGGCCGGGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGcccc                                                                                    <  2:3602242/101‑1 (MQ=255)


                   tttCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGccccc                                                                                   <  1:280276/101‑1 (MQ=255)


                    ttCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGcccccc                                                                                  <  1:2127740/101‑1 (MQ=255)


                     tCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCt                                                                                 <  2:3542157/101‑1 (MQ=255)


                      cATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.TCGcccccccc                                                                                >  1:2208211/1‑99 (MQ=255)


                      cATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTc                                                                                <  2:101023/101‑1 (MQ=255)


                         gAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGTTG.CCGCCCCCCCCGGc                                                                             >  2:513553/1‑101 (MQ=255)


                          aaGGGCCTCCTGCGCGCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAAGGGGGGGGCTTG..CGCCCCCCCCCGcc                                                                            >  1:4883239/1‑101 (MQ=255)


                            gggCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTg                                                                          <  2:2325625/101‑1 (MQ=255)


                              gCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGAc                                                                        <  2:3862249/101‑1 (MQ=255)


                              gCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGAc                                                                        <  1:2990837/101‑1 (MQ=255)


                               cctcctGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACg                                                                       <  1:3144532/101‑1 (MQ=255)


                                ctcctGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCGGG.GCGCGCCCCCCCGcccccccc                                                                      >  1:4123327/1‑95 (MQ=255)


                                ctcctGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCCTG.GCGGCCCCCCCCGCCCGCCgg                                                                      >  2:342688/1‑101 (MQ=255)


                                  cctGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCCCGGCCTGTCGG.cc                                                                   >  2:2764926/1‑101 (MQ=255)


                                  cctGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATAGGGCGCAA.GGGGGGGCTTG.GCGCCCCCCTCCGCCTGACGG.cc                                                                   >  2:447061/1‑101 (MQ=255)


                                     gcgcTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GTGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.Cctct                                                                <  2:1679417/101‑1 (MQ=255)


                                     gcgcTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.Cctct                                                                <  1:3387594/101‑1 (MQ=255)


                                       gcTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGGTGTCGGCCCCCCCCGCCCTG.CGG.ccccctc                                                              >  2:2943515/1‑96 (MQ=255)


                                       gcTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTcc                                                              <  1:695965/101‑1 (MQ=255)


                                         tctgtctgGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTcccc                                                            <  2:4542818/101‑1 (MQ=255)


                                          ctgtctgGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGTTT.GCGCCCCCCCCCGCCCGGCGG.GCCCTccccc                                                           >  1:524921/1‑101 (MQ=255)


                                          ctgtctgGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTccccc                                                           <  1:5548007/101‑1 (MQ=255)


                                          ctgtctgGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTccccc                                                           <  2:3948844/101‑1 (MQ=255)


                                           tgtctgGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTcccccc                                                          <  1:4748948/101‑1 (MQ=255)


                                           tgtctgGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTcccccc                                                          <  1:2277855/101‑1 (MQ=255)


                                            gtctgGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCcg                                                         <  1:3723831/101‑1 (MQ=255)


                                                   cgcgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTAt                                                  <  2:1253883/101‑1 (MQ=255)


                                                    gcgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGTTT.CCGCCCCCCCCGGCCCGCCCG.CCTCTCCCCCCCCCCGGAtt                                                 >  1:3504076/1‑101 (MQ=255)


                                                     cgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.GCGCCCCCCCCCGCCCGGGGG.CCCCTCCGCCCCCGCCGAttt                                                >  1:3875591/1‑101 (MQ=255)


                                                     cgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTAttt                                                <  2:3095638/101‑1 (MQ=255)


                                                     cgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTAttt                                                <  2:3702039/101‑1 (MQ=255)


                                                     cgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTAttt                                                <  1:378818/101‑1 (MQ=255)


                                                      gaattAGCCCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTc                                               <  2:582547/98‑1 (MQ=255)


                                                      gATTTAGCGCAAACAGCCTGAAATGGCGAGATCGGAATCTGGCGCAA.GGTGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTc                                               <  1:4790056/101‑1 (MQ=255)


                                                      gATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTc                                               <  2:4082328/101‑1 (MQ=255)


                                                       aTTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCg                                              <  1:5707040/101‑1 (MQ=255)


                                                        tttAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCCGGCGG.CCCCTCCCCCCCCCCGTCTTTccg                                             >  2:439507/1‑99 (MQ=255)


                                                        tttAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.ACGCCCCCCCAGGGCTGCCGG.ATGCACACCCcgcgcggcactttg                                             >  1:469083/1‑93 (MQ=255)


                                                          tAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGcc                                           <  1:1329099/101‑1 (MQ=255)


                                                           aGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCa                                          <  1:4012276/101‑1 (MQ=255)


                                                             cgcAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGTTT.GCGCCCCCCCCCGCCCGGCGG.CCCCCCCCCCCCCCCCCATTTCCGcccaa                                        >  1:2422346/1‑98 (MQ=255)


                                                             cgcAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCaag                                        <  1:1118475/101‑1 (MQ=255)


                                                             cgcAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCaag                                        <  1:5356455/101‑1 (MQ=255)


                                                              gcAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGTTG.CCGCCCCCCCCGGCCCGACGG.GCCCCCCCCCCCCGCCGATTTTGGCCaaaa                                       >  1:3317110/1‑99 (MQ=255)


                                                               cAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCaagaa                                      <  2:2272096/101‑1 (MQ=255)


                                                               cAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCaagaa                                      <  2:5537889/101‑1 (MQ=255)


                                                                aaaCAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCaagaag                                     <  2:4727142/101‑1 (MQ=255)


                                                                aaaCAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCACCGCGCGTATTTCGGCCaagaag                                     <  1:4657190/101‑1 (MQ=255)


                                                                 aaCAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAagaaga                                    <  1:1848341/101‑1 (MQ=255)


                                                                   cAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGaaa                                  <  1:2598355/101‑1 (MQ=255)


                                                                    aGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAAc                                 <  1:514700/101‑1 (MQ=255)


                                                                       cGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAAcccc                              <  2:5093416/101‑1 (MQ=255)


                                                                        ggAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGTTG.CCGCCCCCCCCCGCCCGACGG.CCCCTCCCCCCCCGCGCATTTTGGCCCACAAGAAAacccc                             >  2:5732701/1‑100 (MQ=255)


                                                                        ggAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCccg                             <  1:1649619/101‑1 (MQ=255)


                                                                            aTGGCGAGGGGGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGGCGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGt                         <  2:1408619/101‑1 (MQ=255)


                                                                             tGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGtt                        >  2:1743309/1‑101 (MQ=255)


                                                                             tGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTC.TCGCCCCCCCCCGCCCGCCGG.GCCCTCCCCCCCCCCCGGTTTTGGGCCAGAAGAAAACcccccggt                        >  2:5624267/1‑99 (MQ=255)


                                                                              ggCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGttt                       <  1:1201553/101‑1 (MQ=255)


                                                                               gCGAGAGCGGAATCGGGCGCAAGGGGGGGGGTTT.CCGCCCCCCCCCCCCGGCGGGCCCTCCCCCCCCCCCCGT.TTTTGGCCAAAAAAAAAccacccccctc                       >  2:2037589/1‑92 (MQ=255)


                                                                                gggggggggAATCGGGCGCAG.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGCCGC.CCCCCCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTttct                     <  1:2757029/94‑1 (MQ=37)


                                                                                cGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCCCCGGttct                     >  2:2798716/1‑101 (MQ=255)


                                                                                  agagCGGAATCGGGCGCAA.GGGGGGGGTTG.CCGCCCCCCCCCGCCCGGCGG.CCCCTCCCCCCCCCCGTCTTTTGGCCCAAAAAAAAACCCCCCCTttcttt                   >  1:257601/1‑100 (MQ=255)


                                                                                        gAATCGGGCGCAA.GGGGGGCCTTG.CCGCCCCCCTTGGCCTACCGG.CCTTCCCCCCCGCGCGTTTTTCGGCCAAGAAGAATCCCCGCCGTTTCTTCTTGGTg             <  1:5757067/101‑1 (MQ=255)


                                                                                          aTCGGGCGCAA.GGGGGGGCTTT.GCGCCCCCCCCGGCCCGACCG.CCCCTCCCCCCCCCCCCATTTTTGCCCAGAAGAAAACCCCCCGTTTCTTCTTTGTGCa           >  2:2439005/1‑101 (MQ=255)


                                                                                          aTCGGGCGCAA.GGGGGGGCTTT.CCGCCCCCCTCCGCCTGACGG.CCTCTCCCCCCGCCCGTCTTTTGGCCAAGAAGAAAACCCGCCGTTTTTTCTTgcggca           >  2:1796137/1‑96 (MQ=255)


                                                                                             gggCGCAG.GGGGGGGCTTG.CCCCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAAt        <  2:5450386/101‑1 (MQ=255)


                                                                                             gggCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCCCCCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAAt        <  1:5108666/101‑1 (MQ=255)


                                                                                               gcgcAG.GGGGGGGCTTC.CCCCCCCCCCCGGCCTGCCGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAATAc      <  2:4294459/101‑1 (MQ=37)


                                                                                               gcgcAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCCCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAATAc      <  1:1816063/101‑1 (MQ=255)


                                                                                                ggcAG.GGGGGGGCTTG.CCCCCCCCCTCGGCCTGGCGG.CCCCCCCCCCCGCGCGTATTTCGGCCAAGAAGAACCCCCGCCGTTTCTTCTTGGTGCAAATAcg     <  2:5356654/100‑1 (MQ=37)


                                                                                                 gcAA.GGGGGGGCTTG.CCGCCCCCCCCGGCCTGAAGG.CCTCTCCCCCCCCGCGTATTTCGGCCCAGAAGAAACCCCCCCGTTTCTTCTTTGTGGCAATAagc    >  2:5280686/1‑101 (MQ=255)


CGGCGGTATCGACGAAGAATTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTG.CCGCCCCCCTCGGCCTGACGG.CCTCTCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAATACGCCC  >  NC_014034/3412009‑3412208

Base quality scores:


ATCG

< 3 ≤


ATCG

< 16 ≤


ATCG

< 27 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG