BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	432,425	+C	100%	intergenic (+28/+120)	RCAP_rcc00403 → / ← RCAP_rcc00404	hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	432,425	1	.→C	100.0%	16.6	228	intergenic (+28/+120)	RCAP_rcc00403/RCAP_rcc00404	hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand): new base (73/147): ref base (7/1): total (80/148)
Fisher's exact test strand distribution p-value = 3.12e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.02e-01

Only 100 of 255 total aligned reads displayed.


GAAATGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGC  >  NC_014034/432325‑432520


gaAATGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGt                                                                                                   >  1:311284/1‑101 (MQ=255)


 aaaTGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.c                                                                                                 >  1:1571980/1‑101 (MQ=255)


  aaTGGCCCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.cc                                                                                                <  2:2885152/101‑2 (MQ=255)


  aaTGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.cc                                                                                                >  1:720991/1‑100 (MQ=255)


    ggggccggggCGGGGGGCGCGATGGGTGGCGATCCGCGCCCGCTTCCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.cctg                                                                                              <  1:4866534/94‑4 (MQ=21)


          cGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACt                                                                                         <  1:1648415/101‑1 (MQ=255)


           gggCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGGGGACTc                                                                                        >  2:1052025/1‑101 (MQ=255)


           gggCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTc                                                                                        <  1:82524/101‑1 (MQ=255)


           gggCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTc                                                                                        >  2:656017/1‑101 (MQ=255)


              cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccc                                                                                     <  2:1912718/101‑1 (MQ=255)


               gcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTccccc                                                                                    <  1:1741125/101‑1 (MQ=255)


                cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccccc                                                                                   <  2:1433348/101‑1 (MQ=255)


                      aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGata                                                                             <  1:3946947/101‑1 (MQ=255)


                      aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGata                                                                             <  1:4058739/101‑1 (MQ=255)


                      aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGata                                                                             <  2:3167694/101‑1 (MQ=255)


                        gATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATAtt                                                                           <  1:1781488/101‑1 (MQ=255)


                        gATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATAtt                                                                           <  1:3398842/101‑1 (MQ=255)


                         aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATAttt                                                                          <  2:2188732/101‑1 (MQ=255)


                         aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATAttt                                                                          <  1:960112/101‑1 (MQ=255)


                          tGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGTGGACTCCCCCCGGGATATTTg                                                                         <  1:3280326/101‑1 (MQ=255)


                            gggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAg                                                                       <  2:464914/101‑1 (MQ=255)


                            ggcatcgatcTGCATACGCTCCCGGCCCCGGCGGGCCACGCGTGAGCCAGGGCAAGGGGACACTGCCCCCCGTCCTGCGGACTACCCCCGGGATATTTGAg                                                                       <  2:5704990/98‑1 (MQ=9)


                              gatcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGc                                                                     <  2:5205691/101‑1 (MQ=255)


                              gatcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGc                                                                     >  2:4314181/1‑101 (MQ=255)


                               atcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCa                                                                    <  1:5715478/101‑1 (MQ=255)


                                    tcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATg                                                               <  2:5317398/101‑1 (MQ=255)


                                     ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGa                                                              <  1:614508/101‑1 (MQ=255)


                                     ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGa                                                              >  1:2231510/1‑101 (MQ=255)


                                     ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGa                                                              <  1:2033708/101‑1 (MQ=255)


                                     ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGCTATTTTAGGCAAGATGa                                                              >  1:252084/1‑101 (MQ=21)


                                      cgcgCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTATTTGAGGCAAGATGaa                                                             >  2:5010943/1‑101 (MQ=255)


                                      cgcgCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaa                                                             <  2:3431418/101‑1 (MQ=255)


                                        cgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAg                                                           <  2:5453115/101‑1 (MQ=255)


                                         gcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGc                                                          <  2:2077447/101‑1 (MQ=255)


                                         gcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGc                                                          <  1:3081866/101‑1 (MQ=255)


                                         gcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAGATTTGAGGCAAGATGAAAGc                                                          >  1:2364160/1‑101 (MQ=255)


                                          cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                         <  2:1252818/101‑1 (MQ=255)


                                           ccggtttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                        <  1:2218575/97‑1 (MQ=255)


                                            cGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.a                                                      <  1:3358956/101‑1 (MQ=255)


                                            cGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.a                                                      <  2:497395/101‑1 (MQ=255)


                                             gCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.At                                                     <  2:1792117/101‑1 (MQ=255)


                                                ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCg                                                  <  2:238664/101‑1 (MQ=255)


                                                 tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCgg                                                 <  2:3724479/101‑1 (MQ=255)


                                                 tCGGCGGCGGAGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCgg                                                 <  1:5488654/101‑1 (MQ=255)


                                                  cggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCcgg                                                >  1:4454289/1‑101 (MQ=255)


                                                   ggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGc                                               <  2:1004843/101‑1 (MQ=255)


                                                   ggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGGTATTTGAGGCAAGATGAAAGCCG.ATCCGGGc                                               >  1:243479/1‑101 (MQ=21)


                                                      ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTc                                            <  1:3723534/101‑1 (MQ=255)


                                                       gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCg                                           <  2:4119138/101‑1 (MQ=255)


                                                        gggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGt                                          <  2:4389382/100‑1 (MQ=255)


                                                          gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGttt                                        <  1:2798056/101‑1 (MQ=255)


                                                           cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTCTTTGAGGGAAGATGAAAGCCG.ATCACGGGTTCGtttt                                       >  2:5165790/1‑100 (MQ=18)


                                                           cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTc                                       <  2:5083619/101‑1 (MQ=255)


                                                            gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCa                                      <  1:3521478/101‑1 (MQ=255)


                                                             ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCAt                                     <  2:2210739/101‑1 (MQ=255)


                                                               ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCt                                   <  1:5462761/101‑1 (MQ=255)


                                                                cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCtt                                  >  1:4331278/1‑101 (MQ=255)


                                                                   gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGcc                               >  2:2341191/1‑101 (MQ=255)


                                                                     atGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGcccc                             >  2:3294165/2‑101 (MQ=255)


                                                                      tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCa                            >  1:2663333/1‑101 (MQ=255)


                                                                       gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCaa                           >  1:1304300/1‑101 (MQ=255)


                                                                        aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCaaa                          <  2:5573872/101‑1 (MQ=255)


                                                                           gcGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGTGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAatat                       <  2:3785604/100‑1 (MQ=255)


                                                                             gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGAATTTGGGTCAAGATGAAAGCCG.ATGCGGGCTTCGGTTCATCTTGCCCCCAATAAcc                     >  1:3983753/1‑101 (MQ=18)


                                                                             gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                     <  2:735686/101‑1 (MQ=255)


                                                                              ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATccc                    <  1:1872491/101‑1 (MQ=255)


                                                                               ncNNGNNGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATcccn                   <  1:2310865/100‑2 (MQ=25)


                                                                                gcggggggCTCTGCCCCCCGG.CTTC..TCTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg       <  1:2690237/99‑3 (MQ=2)


                                                                                cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgg                  >  1:1485863/1‑101 (MQ=255)


                                                                                cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgg                  >  2:4528731/1‑101 (MQ=255)


                                                                                acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg       >  1:5550287/3‑99 (MQ=2)


                                                                                 cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc      >  2:135129/2‑98 (MQ=2)


                                                                                 cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc      >  2:4762476/2‑98 (MQ=2)


                                                                                 aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                 <  1:192117/101‑1 (MQ=255)


                                                                                 aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                 >  1:4243759/1‑101 (MQ=255)


                                                                                   gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggggg               >  2:4166106/1‑101 (MQ=255)


                                                                                   gggggCGCTCCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggggg               <  2:1586684/101‑1 (MQ=255)


                                                                                     gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAAGCG.ATCCGGGCTTCGTTTCATCTTGCCCCCAATATCCCcgggggg             >  1:1046620/1‑99 (MQ=21)


                                                                                      ggCTCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa            <  2:1327564/101‑1 (MQ=255)


                                                                                      ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCCAGATGAAAGCCG.ATCCGGGCTNCGNTNCNTCTTGCCCCCANTATCCCGGGGGGGa            >  1:4984814/1‑101 (MQ=21)


                                                                                      ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa            <  1:920166/101‑1 (MQ=255)


                                                                                       gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa           <  2:4454017/101‑1 (MQ=255)


                                                                                        tctggCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt          <  2:601179/97‑1 (MQ=255)


                                                                                         gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt         <  1:4504679/101‑1 (MQ=255)


                                                                                           tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt       <  1:5046345/101‑1 (MQ=255)


                                                                                           tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt       >  2:2755537/1‑101 (MQ=255)


                                                                                           tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt       >  2:2471759/1‑101 (MQ=255)


                                                                                           tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt       >  1:4142499/1‑101 (MQ=255)


                                                                                           tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt       <  1:3567012/101‑1 (MQ=255)


                                                                                           tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt       <  2:1723904/101‑1 (MQ=255)


                                                                                           tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAAtttt       >  1:4643210/1‑101 (MQ=255)


                                                                                            gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg      <  1:4698144/101‑1 (MQ=255)


                                                                                            gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg      <  2:311251/101‑1 (MQ=255)


                                                                                            gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTg      >  1:4560098/1‑101 (MQ=255)


                                                                                             ccccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTgg     <  1:3177913/101‑1 (MQ=255)


                                                                                             ccccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTgg     <  1:3696895/101‑1 (MQ=255)


                                                                                               ccccGTCCTGCGGACTCCCCCCGGGGTATTTTAGGGAAGATGAAAGACG.ATCCCGGCTTCGTTTTATCTTTCCCCCAACATCCCCGGGGGGAATTTTgggc   >  2:1136278/1‑99 (MQ=16)


                                                                                               ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcg   <  2:1991723/101‑1 (MQ=255)


                                                                                                cccGTCCTGCGGACTCCCCCCGGGGAATTTTAGGGAAAAAGAAAACCGAACCCGGGCTTCGTTTCTTTTTCCCCCAAAATCCCCGGGGGGGATttttttgc   >  1:16629/1‑97 (MQ=255)


                                                                                                cccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcgc  <  2:4025565/101‑1 (MQ=255)


GAAATGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGC  >  NC_014034/432325‑432520

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG