BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,342,925	G→T	1.6%	D421Y (GAC→TAC)	RCAP_rcc01264 →	reverse transcriptase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,342,925	0	G→T	1.6%	4.8 ‑3.7 ‑0.0	303	D421Y (GAC→TAC)	RCAP_rcc01264	reverse transcriptase
Reads supporting (aligned to +/- strand): new base (0/5): ref base (158/140): total (158/145)
Fisher's exact test strand distribution p-value = 2.42e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.48e-01

Only 100 of 316 total aligned reads displayed.


CATCCCCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACG  >  NC_014034/1342825‑1343025


cATCCCCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAg                                                                                                      >  1:1883654/1‑101 (MQ=255)


  tCCCCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGAc                                                                                                    <  1:1350007/101‑1 (MQ=255)


   ccccAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACg                                                                                                   <  2:3515536/101‑1 (MQ=255)


     cttccgatctcAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGaa                                                                                                 <  1:5607888/91‑1 (MQ=255)


     ccAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGaa                                                                                                 <  1:2665077/101‑1 (MQ=255)


      cAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGaaa                                                                                                >  2:4121770/1‑101 (MQ=255)


       aaTTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGAcagatc                                                                                               >  1:4215904/1‑96 (MQ=255)


        aTTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATa                                                                                              <  2:5607076/101‑1 (MQ=255)


        aTTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATa                                                                                              <  1:209207/101‑1 (MQ=255)


         ttttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATaa                                                                                             >  2:5637066/1‑101 (MQ=255)


         ttttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATaa                                                                                             <  1:3011152/101‑1 (MQ=255)


          tttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATaac                                                                                            >  2:3895259/1‑100 (MQ=255)


          tttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATaaa                                                                                            <  1:4386451/101‑1 (MQ=255)


          tttGACAAATATGGCCTTGCGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTACTTTCGGCTCGTGTTGAAAGCATGACGCAAGACGAAATaaa                                                                                            >  2:46050/1‑101 (MQ=255)


           ttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAAc                                                                                           >  1:552134/1‑101 (MQ=255)


           ttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAAc                                                                                           >  1:5667875/1‑101 (MQ=255)


             gACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGc                                                                                         <  1:444251/101‑1 (MQ=255)


             gACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGc                                                                                         >  2:2195220/1‑101 (MQ=255)


                 aaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCa                                                                                     >  2:153071/1‑101 (MQ=255)


                   tatGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCAtt                                                                                   >  1:1710397/1‑101 (MQ=255)


                   tatGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCAtt                                                                                   <  2:5038603/101‑1 (MQ=255)


                    atGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTc                                                                                  <  1:1833579/101‑1 (MQ=255)


                    atGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTc                                                                                  >  1:350866/1‑101 (MQ=255)


                    atGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTc                                                                                  <  1:477039/101‑1 (MQ=255)


                          ttGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACt                                                                            >  2:3782955/1‑101 (MQ=255)


                           tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACtt                                                                           >  1:1431760/1‑101 (MQ=255)


                           tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACtt                                                                           <  1:3203410/101‑1 (MQ=255)


                           tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGAATTCCGAACtt                                                                           >  2:455603/1‑101 (MQ=255)


                            gAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTg                                                                          >  2:4749717/1‑101 (MQ=255)


                               gATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAt                                                                       <  1:996388/101‑1 (MQ=255)


                               gATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAt                                                                       <  2:3224659/101‑1 (MQ=255)


                                aTAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAtt                                                                      >  2:2627980/1‑101 (MQ=255)


                                 tAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTa                                                                     <  2:4973765/101‑1 (MQ=255)


                                   agagGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAAc                                                                   >  2:3192805/1‑101 (MQ=255)


                                       gCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAgg                                                               <  1:65219/101‑1 (MQ=255)


                                       gCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAgg                                                               >  1:36402/1‑101 (MQ=255)


                                         gagaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGCGTTGAAAGCATGACGGCAGAGGAAAAAAACGGTGCATTCCGAACGTGCGTGAAAAACGaa                                                             >  2:1677897/1‑101 (MQ=255)


                                           gaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAt                                                           <  1:2766595/101‑1 (MQ=255)


                                           gaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAt                                                           <  1:2026402/101‑1 (MQ=255)


                                           gaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAt                                                           <  1:3395558/101‑1 (MQ=255)


                                            atatTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAtc                                                          <  2:2013136/101‑1 (MQ=255)


                                            atatTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAtc                                                          >  1:5374062/1‑101 (MQ=255)


                                            atatTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAta                                                          >  2:659550/1‑100 (MQ=255)


                                             tatTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAtct                                                         <  1:4672756/101‑1 (MQ=255)


                                             tatTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAtct                                                         <  1:4000439/101‑1 (MQ=255)


                                               tttGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCg                                                       >  1:30976/1‑101 (MQ=255)


                                                ttGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCgg                                                      >  2:2708232/1‑101 (MQ=255)


                                                ttGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCgg                                                      >  1:4642943/1‑101 (MQ=255)


                                                  gTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGat                                                    >  1:5234772/1‑101 (MQ=255)


                                                  gTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGat                                                    >  2:2370476/1‑101 (MQ=255)


                                                   tCTGCTTGCCCCCCACGTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGata                                                   <  2:2844651/101‑1 (MQ=255)


                                                    ctgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGatat                                                  >  1:4558690/1‑101 (MQ=255)


                                                     tgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATc                                                 <  1:4871992/101‑1 (MQ=255)


                                                     tgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATc                                                 <  1:2188011/101‑1 (MQ=255)


                                                     tgctgGCCCCCAACTTATTTTCTGCTCGTTTTAAAAACAAGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATc                                                 <  1:5433314/101‑1 (MQ=255)


                                                      gctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCg                                                >  2:5140040/1‑101 (MQ=255)


                                                      gctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCg                                                >  1:5482613/1‑101 (MQ=255)


                                                      gctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCg                                                >  2:4275192/1‑101 (MQ=255)


                                                       ctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCga                                               <  1:5050465/101‑1 (MQ=255)


                                                       ctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCga                                               >  2:4503177/1‑101 (MQ=255)


                                                        tgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCgag                                              >  1:2265974/1‑101 (MQ=255)


                                                        tgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCgag                                              >  2:160571/1‑101 (MQ=255)


                                                          gCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGaa                                            >  2:4208375/1‑101 (MQ=255)


                                                            cccccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAg                                          >  1:1514961/1‑101 (MQ=255)


                                                            cccccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAg                                          >  1:576111/1‑101 (MQ=255)


                                                               ccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGAcc                                       >  2:4385739/1‑101 (MQ=255)


                                                                 aCTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTg                                     >  2:3607643/1‑101 (MQ=255)


                                                                   ttATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCt                                   >  1:1115415/1‑101 (MQ=255)


                                                                   ttATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCt                                   >  1:2459228/1‑101 (MQ=255)


                                                                    tATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCtt                                  <  2:552084/101‑1 (MQ=255)


                                                                     aTTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTg                                 >  1:2338895/1‑101 (MQ=255)


                                                                     aTTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTg                                 >  1:795210/1‑101 (MQ=255)


                                                                       tttCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAg                               <  2:4159542/101‑1 (MQ=255)


                                                                        ttCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAgg                              <  2:1785084/101‑1 (MQ=255)


                                                                        ttCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAgg                              >  1:911613/1‑101 (MQ=255)


                                                                         tCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGc                             >  1:2291877/1‑101 (MQ=255)


                                                                         tCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGc                             >  2:3055962/1‑101 (MQ=255)


                                                                          cTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCa                            >  1:411077/1‑101 (MQ=255)


                                                                           tGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCAt                           <  1:1948861/101‑1 (MQ=255)


                                                                                  gtTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTa                    >  1:174189/1‑101 (MQ=255)


                                                                                  gtTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTa                    >  1:3809441/1‑101 (MQ=255)


                                                                                   ttGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTaa                   >  1:1650273/1‑101 (MQ=255)


                                                                                   ttGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTaa                   <  2:2080682/101‑1 (MQ=255)


                                                                                   ttGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTaa                   >  2:3324773/1‑101 (MQ=255)


                                                                                    tGAAAGCATGACGGCAGACGGAATAAAAGCTGCACTCCGGATGTGACTTAACAAGGAAATATCGGATATCAGGAAATCCATGCTATAGACATTTTCGttct                  >  2:1303231/1‑98 (MQ=255)


                                                                                    tGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAAt                  <  1:387972/101‑1 (MQ=255)


                                                                                     gAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATg                 >  1:1962957/1‑101 (MQ=255)


                                                                                       aaGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGtt               >  1:5307457/1‑101 (MQ=255)


                                                                                          cATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGc            >  2:1383435/1‑101 (MQ=255)


                                                                                          cATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGc            >  2:1752109/1‑101 (MQ=255)


                                                                                           aTGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGcc           >  2:1951534/1‑101 (MQ=255)


                                                                                           aTGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGcc           >  2:2599171/1‑101 (MQ=255)


                                                                                             gACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAg         <  2:5373772/101‑1 (MQ=255)


                                                                                                ggCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAAt      <  2:2032159/101‑1 (MQ=255)


                                                                                                 gCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATg     <  2:2459094/101‑1 (MQ=255)


                                                                                                  cAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGa    <  1:4840760/101‑1 (MQ=255)


                                                                                                  cAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGa    >  2:1499795/1‑101 (MQ=255)


                                                                                                   aGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGAc   <  2:1205684/101‑1 (MQ=255)


                                                                                                    gACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGCCg  >  2:806550/1‑101 (MQ=255)


                                                                                                    gACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACg  <  1:1227089/101‑1 (MQ=255)


CATCCCCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACG  >  NC_014034/1342825‑1343025

Base quality scores:


ATCG

< 3 ≤


ATCG

< 26 ≤


ATCG

< 32 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG