BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,342,930	A→G	1.9%	E422E (GAA→GAG)	RCAP_rcc01264 →	reverse transcriptase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,342,930	0	A→G	1.9%	2.6 ‑4.5 ‑0.0	300	E422E (GAA→GAG)	RCAP_rcc01264	reverse transcriptase
Reads supporting (aligned to +/- strand): new base (0/6): ref base (157/137): total (157/143)
Fisher's exact test strand distribution p-value = 1.11e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 317 total aligned reads displayed.


CCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGTAGGG  >  NC_014034/1342830‑1343030


ccAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGaa                                                                                                      <  2:2455358/101‑1 (MQ=255)


ccAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGaa                                                                                                      <  1:2665077/101‑1 (MQ=255)


   aTTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATa                                                                                                   <  2:5607076/101‑1 (MQ=255)


    ttttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATaa                                                                                                  >  2:5637066/1‑101 (MQ=255)


    ttttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATaa                                                                                                  >  2:4146825/1‑101 (MQ=255)


    ttttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATaa                                                                                                  <  1:3011152/101‑1 (MQ=255)


      ttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAAc                                                                                                <  1:5745265/101‑1 (MQ=255)


      ttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAAc                                                                                                <  1:4800950/101‑1 (MQ=255)


       tGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACg                                                                                               >  2:4767376/1‑101 (MQ=255)


           aaaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGc                                                                                           <  1:4398646/101‑1 (MQ=255)


           aaaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGc                                                                                           <  1:3849182/101‑1 (MQ=255)


              tatGGGGTTGAGGATAAGAGGCGAGATATTTGTCTGGTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCATACGATATAAACGCTGTAtt                                                                                        >  2:1925416/1‑101 (MQ=255)


               atGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCGCAACTTATTTTCTGCTCGTGTTGAAAGCATGTCGGAAGACGAAATAAACGCTgacgtc                                                                                       >  2:2580818/1‑96 (MQ=255)


               atGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTc                                                                                       >  1:350866/1‑101 (MQ=255)


                tGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTcc                                                                                      <  2:5333458/101‑1 (MQ=255)


                 gggCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCg                                                                                     <  1:4161825/101‑1 (MQ=255)


                   gCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGGCGGCAGACGAAATAAACGCTGCATTCCGaa                                                                                   <  1:3895501/101‑1 (MQ=255)


                     ttGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACt                                                                                 >  1:5608876/1‑101 (MQ=255)


                     ttGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACt                                                                                 <  1:176653/101‑1 (MQ=255)


                      tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACtt                                                                                <  1:3203410/101‑1 (MQ=255)


                      tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGAATTCCGAACtt                                                                                >  2:455603/1‑101 (MQ=255)


                       gAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTg                                                                               >  2:2615811/1‑101 (MQ=255)


                       gAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTg                                                                               <  2:212850/101‑1 (MQ=255)


                        aGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGc                                                                              >  1:4561844/1‑101 (MQ=255)


                            tAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTa                                                                          >  1:1567696/1‑101 (MQ=255)


                            tAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTa                                                                          <  1:4539316/101‑1 (MQ=255)


                            tAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTa                                                                          <  1:1408025/101‑1 (MQ=255)


                            tAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTa                                                                          <  2:5054661/101‑1 (MQ=255)


                              agagGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAAc                                                                        >  1:2223811/1‑101 (MQ=255)


                              agagGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAAc                                                                        >  1:2080799/1‑101 (MQ=255)


                               gagGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACa                                                                       >  2:1279538/1‑101 (MQ=255)


                                 ggCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAg                                                                     <  1:2116027/101‑1 (MQ=255)


                                  gCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAgg                                                                    >  1:36402/1‑101 (MQ=255)


                                  gCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAgg                                                                    <  1:65219/101‑1 (MQ=255)


                                    gagaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGaa                                                                  >  2:2314085/1‑101 (MQ=255)


                                    gagaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGCGTTGAAAGCATGACGGCAGAGGAAAAAAACGGTGCATTCCGAACGTGCGTGAAAAACGaa                                                                  >  2:1677897/1‑101 (MQ=255)


                                      gaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAt                                                                <  1:2766595/101‑1 (MQ=255)


                                       atatTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAtc                                                               <  2:2045875/101‑1 (MQ=255)


                                       atatTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAta                                                               >  2:659550/1‑100 (MQ=255)


                                        tatTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAtct                                                              >  2:1961896/1‑101 (MQ=255)


                                         atTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAtctc                                                             <  1:2022945/101‑1 (MQ=255)


                                          tttGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCg                                                            <  1:2314211/101‑1 (MQ=255)


                                          tttGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCg                                                            >  1:30976/1‑101 (MQ=255)


                                           ttGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCgg                                                           <  1:5799379/101‑1 (MQ=255)


                                            tGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGa                                                          <  1:1854187/101‑1 (MQ=255)


                                             gTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGat                                                         <  1:1660892/101‑1 (MQ=255)


                                               ctgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGatat                                                       >  2:3818652/1‑101 (MQ=255)


                                               ctgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGatat                                                       >  2:3115945/1‑101 (MQ=255)


                                                 gctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCg                                                     >  2:5140040/1‑101 (MQ=255)


                                                 gctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCg                                                     >  1:5436361/1‑101 (MQ=255)


                                                 gctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCg                                                     >  2:2794359/1‑101 (MQ=255)


                                                 gctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCg                                                     >  2:4275192/1‑101 (MQ=255)


                                                  ctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCga                                                    >  2:4503177/1‑101 (MQ=255)


                                                  ctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCga                                                    <  1:5050465/101‑1 (MQ=255)


                                                  ctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCga                                                    >  2:420558/1‑101 (MQ=255)


                                                   tgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCgag                                                   >  2:160571/1‑101 (MQ=255)


                                                     gCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGaa                                                 <  2:1486927/101‑1 (MQ=255)


                                                      ccccccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGaaa                                                >  2:3694443/1‑101 (MQ=255)


                                                       cccccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAg                                               >  1:3434334/1‑101 (MQ=255)


                                                         cccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGAc                                             <  1:1452183/101‑1 (MQ=255)


                                                         cccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGAc                                             <  1:5238327/101‑1 (MQ=255)


                                                          tccgattttttCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGAcc                                            >  2:1368168/8‑101 (MQ=255)


                                                          ccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGAcc                                            >  2:4385739/1‑101 (MQ=255)


                                                             cTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGc                                         <  1:4275452/101‑1 (MQ=255)


                                                              ttATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCt                                        >  1:1115415/1‑101 (MQ=255)


                                                               tATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCtt                                       <  2:552084/101‑1 (MQ=255)


                                                                 ttttCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGa                                     <  1:2539437/101‑1 (MQ=255)


                                                                   ttCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAgg                                   <  2:1785084/101‑1 (MQ=255)


                                                                   ttCTGCTCGTGTTGAAAGCATAACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAgg                                   <  1:987474/101‑1 (MQ=255)


                                                                    tCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGc                                  <  2:2382554/101‑1 (MQ=255)


                                                                        cTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCAttt                              >  1:1724431/1‑101 (MQ=255)


                                                                        cTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCAttt                              >  1:823070/1‑101 (MQ=255)


                                                                          cGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTc                            >  1:540453/1‑101 (MQ=255)


                                                                              ttGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTaa                        <  1:5566262/101‑1 (MQ=255)


                                                                              ttGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTaa                        >  2:3324773/1‑101 (MQ=255)


                                                                               tGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAAt                       >  2:2675109/1‑101 (MQ=255)


                                                                               tGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAAt                       >  1:5497846/1‑101 (MQ=255)


                                                                                gAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATg                      >  1:4664755/1‑101 (MQ=255)


                                                                                gAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATg                      >  2:4691891/1‑101 (MQ=255)


                                                                                gAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATg                      <  1:1931851/101‑1 (MQ=255)


                                                                                 aaaGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGt                     >  2:4976460/1‑101 (MQ=255)


                                                                                     cATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGc                 <  1:5329294/101‑1 (MQ=255)


                                                                                     cATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGc                 >  2:1752109/1‑101 (MQ=255)


                                                                                       tGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCa               <  2:3567243/101‑1 (MQ=255)


                                                                                        gACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAg              >  2:3202593/1‑101 (MQ=255)


                                                                                          cGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGaa            >  2:4066080/1‑101 (MQ=255)


                                                                                          cGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGaa            >  2:2917169/1‑101 (MQ=255)


                                                                                            gCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATg          <  1:1962007/101‑1 (MQ=255)


                                                                                             cAGACGCAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGa         <  2:1130408/101‑1 (MQ=255)


                                                                                             cAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGa         <  1:2288746/101‑1 (MQ=255)


                                                                                               gACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGCCg       >  2:806550/1‑101 (MQ=255)


                                                                                               gACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACg       >  1:4856335/1‑101 (MQ=255)


                                                                                               gACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACg       <  1:1227089/101‑1 (MQ=255)


                                                                                               gACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACg       <  2:1514868/101‑1 (MQ=255)


                                                                                                aCGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGt      >  2:4002497/1‑101 (MQ=255)


                                                                                                  gAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGTAg    >  1:4725807/1‑101 (MQ=255)


                                                                                                  gAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGTAg    >  1:4828533/1‑101 (MQ=255)


                                                                                                  gAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGTAg    <  1:771359/101‑1 (MQ=255)


                                                                                                   aaaTAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGTAgg   <  1:2370610/101‑1 (MQ=255)


                                                                                                    aaTAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGTAggg  <  1:4043019/101‑1 (MQ=255)


CCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGTAGGG  >  NC_014034/1342830‑1343030

Base quality scores:


ATCG

< 3 ≤


ATCG

< 25 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG