Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,556 C→G 13.2% G146G (GGC→GGG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5560C→G13.2% 104.8 ‑2.2 ‑0.1332G146G (GGC→GGGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/26):  ref base (156/132):  total (174/158)
Fisher's exact test strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.81e-01

Only 100 of 358 total aligned reads displayed.

CGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGG  >  NC_014034/1396457‑1396656
                                                                                                    |                                                                                                    
cGTNGNTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGcc                                                                                                     <  1:1538760/101‑1 (MQ=255)
 gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                                    <  2:2047553/101‑1 (MQ=255)
  tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAg                                                                                                   <  2:4656268/101‑1 (MQ=255)
  tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAg                                                                                                   <  1:2452509/101‑1 (MQ=255)
    gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                                 <  1:1508105/101‑1 (MQ=255)
    gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                                 >  2:2388400/1‑101 (MQ=255)
    gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                                 >  2:4238463/1‑101 (MQ=255)
    gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACAGGCCAgcc                                                                                                 >  2:5639341/1‑101 (MQ=255)
       cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccgcc                                                                                              <  1:459103/101‑1 (MQ=255)
        gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccgccg                                                                                             <  2:5077635/101‑2 (MQ=255)
         aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                                            >  1:73115/1‑101 (MQ=255)
         aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                                            >  1:177192/1‑101 (MQ=255)
             ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGcc                                                                                        <  2:4102540/101‑1 (MQ=255)
                ccTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAg                                                                                     <  1:3803614/101‑1 (MQ=255)
                 cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGt                                                                                    >  1:5339070/1‑101 (MQ=255)
                  tGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTc                                                                                   <  1:2280801/101‑1 (MQ=255)
                   gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCg                                                                                  >  1:1333043/1‑101 (MQ=255)
                      atgccGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                               <  1:424896/98‑1 (MQ=255)
                      aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                               >  1:4161580/1‑101 (MQ=255)
                        tCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccc                                                                             <  1:395038/101‑1 (MQ=255)
                          cgcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccccc                                                                           >  2:4921428/1‑101 (MQ=255)
                          cgcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccccc                                                                           <  1:4635755/101‑1 (MQ=255)
                           gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                          >  1:1037161/1‑101 (MQ=255)
                           gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                          <  1:2861442/101‑1 (MQ=255)
                             gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgc                                                                        >  2:1835181/1‑101 (MQ=255)
                                aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg                                                                     >  1:1107208/1‑101 (MQ=255)
                                 aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                                    >  1:4905640/1‑101 (MQ=255)
                                 aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                                    >  2:4849641/1‑101 (MQ=255)
                                    gcggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCg                                                                 >  2:713690/1‑101 (MQ=255)
                                    gcggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCg                                                                 >  1:3034521/1‑101 (MQ=255)
                                      ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCggg                                                               >  1:1968921/1‑99 (MQ=255)
                                        cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                             >  2:5578806/1‑101 (MQ=255)
                                         ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            >  2:5738638/1‑101 (MQ=255)
                                          gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                            >  2:309842/9‑101 (MQ=2)
                                          gagAGCACGTCGCGCCTCCCGCGGGGTCGTTGATCGATACTGCTTTC.GTGGGACTGGCCAGCCTCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  2:296698/99‑1 (MQ=37)
                                            gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                          <  2:3652460/95‑1 (MQ=2)
                                            gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         >  1:3376182/1‑101 (MQ=255)
                                             acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                         >  2:3353445/6‑101 (MQ=2)
                                               ggaggcGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                       <  2:1755427/96‑1 (MQ=2)
                                                gagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGGTCGAAAc                                                      >  2:8490/3‑101 (MQ=2)
                                                aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                     >  1:880009/1‑101 (MQ=255)
                                                 agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                     >  2:1637317/2‑100 (MQ=2)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                   >  1:1929139/1‑101 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActg                                                    >  2:5345509/1‑99 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                 <  1:1675793/101‑1 (MQ=255)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                 <  1:2964067/101‑1 (MQ=255)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                  >  1:2177471/1‑97 (MQ=2)
                                                    cGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGCCTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                 <  2:1347690/101‑1 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                >  1:4964078/1‑101 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                >  2:4758556/1‑101 (MQ=255)
                                                      cTCCCCCGGCGGGGGCGTGGATCGAAACTTCTTTC.GTGGGACTGGCCACCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                               <  1:2932799/101‑1 (MQ=255)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                               <  1:521673/101‑1 (MQ=255)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                              <  1:4288138/101‑1 (MQ=255)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAGATTtcgt                                              >  2:4542645/1‑101 (MQ=255)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                               <  2:3718966/101‑8 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                             <  1:1830719/101‑1 (MQ=255)
                                                        ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                              <  1:3640680/101‑9 (MQ=2)
                                                         cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                             <  1:5051291/101‑10 (MQ=2)
                                                          ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                                            >  2:4977551/1‑91 (MQ=2)
                                                           cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                                          >  2:2124197/1‑101 (MQ=255)
                                                             cgcgGGGTCGTGGATCGAAACTGCTTTC.GGGGGACTGGCCAGTAGCCTGCGACAAGCCGCTCCCCCCGCGGGCGCGTGGGTCGAAACGATTTCGCCGCTTa                                        >  2:2563605/1‑101 (MQ=25)
                                                              gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                       <  2:3792378/101‑1 (MQ=255)
                                                              gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                       >  2:3498438/1‑101 (MQ=255)
                                                                     cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                >  2:1022526/1‑101 (MQ=255)
                                                                      gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                               >  2:2318996/1‑101 (MQ=255)
                                                                      gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                               >  2:660806/1‑101 (MQ=255)
                                                                      gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                               >  1:2418782/1‑101 (MQ=255)
                                                                       tGGATCGAAACTGCTTGC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                              <  1:2035679/101‑1 (MQ=255)
                                                                        ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                             >  1:685797/1‑101 (MQ=255)
                                                                         gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                            >  1:1405054/1‑101 (MQ=255)
                                                                         gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                            >  2:4588188/1‑101 (MQ=255)
                                                                         gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                            <  2:779781/101‑1 (MQ=255)
                                                                         gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                            <  1:35182/101‑1 (MQ=255)
                                                                          aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                           >  2:5795011/1‑101 (MQ=255)
                                                                          aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                           >  2:3929957/1‑101 (MQ=255)
                                                                           tCGAACATGCTTTC.GTGGGACTGGCCGGCGGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                          <  2:3560092/101‑1 (MQ=38)
                                                                            cGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                         <  2:3244785/101‑1 (MQ=255)
                                                                             gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                        >  1:567602/1‑101 (MQ=255)
                                                                              aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                       >  2:2733531/1‑101 (MQ=255)
                                                                              aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                       >  2:1086580/1‑101 (MQ=255)
                                                                               aaCTGCTTTC.GTGGGACTGGCCAGCCGCACGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc                      <  1:5308965/101‑1 (MQ=255)
                                                                                 cTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg                    >  2:2877766/1‑101 (MQ=255)
                                                                                 cTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg                    >  2:291720/1‑101 (MQ=255)
                                                                                  tGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg                   <  2:5213123/101‑1 (MQ=255)
                                                                                  tGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg                   <  2:3407847/101‑1 (MQ=255)
                                                                                  tGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg                   <  1:2893667/101‑1 (MQ=255)
                                                                                   gCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                  >  2:4006075/1‑101 (MQ=255)
                                                                                    cTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc                 <  1:2124313/101‑1 (MQ=255)
                                                                                     tttC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                >  1:5474068/1‑101 (MQ=255)
                                                                                     tttC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCCTCGCTTACGACGCCTATCTGATTTCGGGTCg                <  1:5125710/101‑1 (MQ=255)
                                                                                     tctgcttGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc                 >  2:2517207/7‑101 (MQ=255)
                                                                                           tGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc           <  2:441654/101‑1 (MQ=255)
                                                                                            gggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc          >  2:449023/1‑101 (MQ=255)
                                                                                             ggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccc         >  1:81525/1‑101 (MQ=255)
                                                                                              gccTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccccc        <  2:1496185/99‑1 (MQ=255)
                                                                                                 tGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg     >  1:2499184/1‑101 (MQ=255)
                                                                                                 tGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg     >  2:1166860/1‑101 (MQ=255)
                                                                                                  ggCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgg    >  1:3158435/1‑101 (MQ=255)
                                                                                                   gCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg   <  1:2653785/101‑1 (MQ=255)
                                                                                                    ccAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgggg  <  1:5582103/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGG  >  NC_014034/1396457‑1396656

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG