BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,803	C→G	10.2%	R229G (CGT→GGC)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,803	0	C→G	10.2%	71.3 ‑0.6 ‑0.0	316	R229G (CGT→GGT)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (19/13): ref base (149/134): total (168/148)
Fisher's exact test strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.94e-01

Only 100 of 333 total aligned reads displayed.


ATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACG  >  NC_014034/1396703‑1396903


aTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..Acc                                                                                                           <  1:4813640/101‑1 (MQ=255)


  cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.gt                                                                                                        <  1:3394462/101‑1 (MQ=255)


   gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.gtg                                                                                                       >  1:2320877/1‑101 (MQ=255)


      gCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTgg                                                                                                    <  1:2551237/101‑1 (MQ=255)


      gCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTgg                                                                                                    >  2:4599846/1‑101 (MQ=255)


       cTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGga                                                                                                   <  2:436409/101‑1 (MQ=255)


        tCCCTCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGgat                                                                                                  >  2:183450/1‑101 (MQ=255)


        tCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGgat                                                                                                  >  2:3757954/1‑101 (MQ=255)


        tCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGgat                                                                                                  >  1:2868664/1‑101 (MQ=255)


         ccccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTCTGgat...g                                                                                              >  2:4454277/1‑101 (MQ=255)


          cccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGgat...ga                                                                                             <  1:647866/101‑1 (MQ=255)


          cccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGGGgat...ga                                                                                             >  1:1537739/1‑101 (MQ=255)


            cccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATc                                                                                           <  2:2411352/101‑1 (MQ=255)


            cccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGCATCGAA..ACC.GTGTGGAT...GATc                                                                                           <  1:4259788/101‑1 (MQ=255)


               gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATg                                                                                        >  2:495845/1‑101 (MQ=255)


               gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATg                                                                                        >  1:2784509/1‑101 (MQ=255)


                  ggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGTGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAg                                                                                     <  1:5763584/101‑1 (MQ=255)


                   tgtcagagATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAgc                                                                                    >  2:2968181/8‑101 (MQ=255)


                     gCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAgc.gc                                                                                 >  1:4941800/1‑101 (MQ=255)


                       gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCgg                                                                               >  1:1611753/1‑101 (MQ=255)


                        tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCggc                                                                              >  1:5268747/1‑100 (MQ=255)


                        tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGa                                                                              >  1:1325163/1‑101 (MQ=255)


                          gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACg                                                                            <  2:2016710/101‑1 (MQ=255)


                          gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACg                                                                            <  1:2996682/101‑1 (MQ=255)


                          gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACg                                                                            <  2:337781/101‑1 (MQ=255)


                           aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACgg                                                                           >  1:3087811/1‑101 (MQ=255)


                              caaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCtt                                                                        <  2:784516/100‑1 (MQ=255)


                               aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTg                                                                       >  1:1781385/1‑101 (MQ=255)


                               aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTg                                                                       >  1:4755856/1‑101 (MQ=255)


                               aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTg                                                                       >  1:886512/1‑101 (MQ=255)


                                aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTgg                                                                      <  2:3008413/101‑1 (MQ=255)


                                aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTgg                                                                      <  2:2320751/101‑1 (MQ=255)


                                   ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTc                                                                   <  2:3362961/101‑1 (MQ=255)


                                   ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTc                                                                   <  2:3118728/101‑1 (MQ=255)


                                   ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTc                                                                   <  1:4600122/101‑1 (MQ=255)


                                    cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGCCGGCTTGGGTCg                                                                  >  2:2710912/1‑101 (MQ=255)


                                    cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCg                                                                  <  2:5568443/101‑1 (MQ=255)


                                    cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCg                                                                  <  1:556020/101‑1 (MQ=255)


                                       tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTc                                                               >  1:5325777/1‑101 (MQ=255)


                                        cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTcc                                                              <  1:5464321/101‑1 (MQ=255)


                                          cttatatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCTAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTcccc                                                            <  2:4266134/98‑1 (MQ=255)


                                          cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTcccc                                                            >  1:2148448/1‑101 (MQ=255)


                                            cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTcccccc                                                          >  1:3178757/1‑101 (MQ=255)


                                            cATATGCTGCCACGANGCCCTTGAGTCGCTCCCCCCGCGGGGGNGTGGATCGAA..ACC.GTGTGGAT...GATNANGCNGC.GCTGANGNNTNGNNNCNCTcccccc                                                          >  1:1905882/1‑101 (MQ=37)


                                               atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCCCCCCCcgcg                                                       >  2:1685223/1‑101 (MQ=255)


                                                 gcggcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCggg                                                     <  1:1460759/98‑1 (MQ=255)


                                                  ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCgggg                                                    >  1:4121894/1‑101 (MQ=255)


                                                   tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                   >  1:2128821/1‑101 (MQ=255)


                                                   tgacACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                   <  2:4875597/98‑1 (MQ=255)


                                                    gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGGCGCTCCCCCCGCGGGGGc                                                  >  1:5130001/1‑101 (MQ=255)


                                                         gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  2:4722288/1‑101 (MQ=255)


                                                          tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                              <  1:5611426/91‑1 (MQ=2)


                                                          aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  2:685740/101‑1 (MQ=255)


                                                          aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                            >  2:1592739/1‑101 (MQ=255)


                                                           cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  2:1435788/10‑101 (MQ=0)


                                                              ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  2:3309415/1‑101 (MQ=255)


                                                              ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:2702821/101‑1 (MQ=255)


                                                              atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  1:3680693/7‑101 (MQ=2)


                                                              atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  2:595636/7‑101 (MQ=2)


                                                                ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGGGGGGGTCGAAAc                                      >  1:4424251/1‑101 (MQ=255)


                                                                 tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                     <  2:1224984/101‑1 (MQ=255)


                                                                 gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  2:5403304/4‑101 (MQ=2)


                                                                 gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  2:2810042/4‑101 (MQ=2)


                                                                  gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACCg                                    >  1:1187353/1‑101 (MQ=255)


                                                                  gAGGCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GGTTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                    <  1:4029656/101‑1 (MQ=255)


                                                                   cgTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                     >  1:1051622/2‑100 (MQ=2)


                                                                    ggcGCTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                    <  1:52/99‑3 (MQ=2)


                                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA...AAAAAGGCAGCAGCAG.CGGCTGCGGTCGCTCCCCCCGAGGGGGGGTGGATCGaaaaa                                     >  2:4077315/1‑99 (MQ=0)


                                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGt                                  >  1:224627/1‑101 (MQ=255)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                   <  2:2085173/101‑4 (MQ=2)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                  >  2:384415/1‑97 (MQ=2)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                <  2:1274607/101‑1 (MQ=255)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                               <  2:5270861/101‑1 (MQ=255)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                               <  2:5036019/101‑1 (MQ=255)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                >  1:2441555/1‑95 (MQ=2)


                                                                         tctccccGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                             <  2:5319968/98‑1 (MQ=255)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                               <  1:4300806/101‑8 (MQ=2)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAA..ACG.GCGTAGATCTCGGTCTGGGTGC.GCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                               >  1:3800141/1‑94 (MQ=2)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                             >  1:854501/1‑101 (MQ=255)


                                                                           cccccGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                           >  1:2387736/1‑101 (MQ=255)


                                                                                  gggggCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                    >  1:2843677/1‑101 (MQ=255)


                                                                                    gggCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcg                  <  2:5745186/101‑1 (MQ=255)


                                                                                      gCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgcg                >  2:1784375/1‑101 (MQ=255)


                                                                                      gCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgcg                >  1:1248913/1‑101 (MQ=255)


                                                                                      gCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgcg                >  1:736064/1‑101 (MQ=255)


                                                                                        gTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa              >  2:1957000/1‑101 (MQ=255)


                                                                                        gTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa              >  1:3938777/1‑101 (MQ=255)


                                                                                         tGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc             <  2:231389/101‑1 (MQ=255)


                                                                                         tGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc             <  2:5628101/101‑1 (MQ=255)


                                                                                         tGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCAGTCCAGAGATAGACCGCGCGGAc             >  2:1498089/1‑101 (MQ=255)


                                                                                           gATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct           >  2:257118/1‑101 (MQ=255)


                                                                                           gATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct           >  2:1369601/1‑101 (MQ=255)


                                                                                             tCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcc         <  1:3465768/101‑1 (MQ=255)


                                                                                                aa..aCC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa      >  1:3331818/1‑101 (MQ=255)


                                                                                                 a..aCC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg     >  1:3755478/1‑101 (MQ=255)


                                                                                                 a..aCC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg     >  1:5410728/1‑101 (MQ=255)


                                                                                                 a..aCC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg     >  1:2655490/1‑101 (MQ=255)


                                                                                                 a..aCC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg     >  1:983206/1‑101 (MQ=255)


                                                                                                      c.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg  <  1:1816929/101‑1 (MQ=255)


                                                                                                      c.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg  <  2:3512553/101‑1 (MQ=255)


ATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA..ACC.GTGTGGAT...GATCATGCAGC.GCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACG  >  NC_014034/1396703‑1396903

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG