Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,805 T→C 10.6% R229R (CGT→GGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8050T→C10.6% 66.9 ‑1.0 ‑0.5310R229R (CGT→CGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/13):  ref base (142/132):  total (162/148)
Fisher's exact test strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.87e-01

Only 100 of 336 total aligned reads displayed.

CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTC  >  NC_014034/1396705‑1396905
                                                                                                    |                                                                                                        
 gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgtg                                                                                                         >  2:2464163/1‑101 (MQ=255)
  tcgCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgtg.t                                                                                                       <  1:5457647/101‑1 (MQ=255)
   cgCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGtg.tg                                                                                                      <  2:2706025/101‑1 (MQ=255)
    gCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.Tgg                                                                                                     <  2:3208752/101‑1 (MQ=255)
     cTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGga                                                                                                    <  2:436409/101‑1 (MQ=255)
      tCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat                                                                                                   >  2:3757954/1‑101 (MQ=255)
      tCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat                                                                                                   >  1:2868664/1‑101 (MQ=255)
       ccccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat...g                                                                                               >  1:5530804/1‑101 (MQ=255)
        ttccgatctgggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat...ga                                                                                              <  1:786467/92‑1 (MQ=255)
        cccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.GGgat...ga                                                                                              >  1:1537739/1‑101 (MQ=255)
         ccccGCGGGGGCGTAGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTGGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat...gat                                                                                             <  2:2763958/101‑1 (MQ=255)
          cccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGCATCGAAACCGTG.TGGAT...GATc                                                                                            <  1:4259788/101‑1 (MQ=255)
             gcgGGGGCGTGGATCGAAGCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATg                                                                                         <  2:5637821/101‑1 (MQ=255)
             gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATg                                                                                         >  2:495845/1‑101 (MQ=255)
             gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATg                                                                                         >  2:5361940/1‑101 (MQ=255)
                ggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAg                                                                                      <  2:1537645/101‑1 (MQ=255)
                 gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat...gaagatcggaa                                                                                     >  2:786399/1‑92 (MQ=255)
                    cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGcgcg                                                                                  >  1:5036302/1‑101 (MQ=255)
                      tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGa                                                                                >  2:1816470/1‑101 (MQ=255)
                        gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACg                                                                              <  2:337781/101‑1 (MQ=255)
                         aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACgg                                                                             >  1:2763709/1‑101 (MQ=255)
                         aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACgg                                                                             >  1:3087811/1‑101 (MQ=255)
                            gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                          <  1:5251369/101‑1 (MQ=255)
                             aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTg                                                                         <  2:1781286/101‑1 (MQ=255)
                             aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTg                                                                         <  2:4857807/101‑1 (MQ=255)
                             aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTg                                                                         >  1:4755856/1‑101 (MQ=255)
                             aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTg                                                                         >  1:1781385/1‑101 (MQ=255)
                             aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTg                                                                         <  1:3370817/101‑1 (MQ=255)
                             aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTg                                                                         >  1:2004590/1‑101 (MQ=255)
                              accccTGTCCCTCATATGCGGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTgg                                                                        <  2:4142312/99‑1 (MQ=255)
                                 ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTggggc                                                                     >  1:854839/1‑99 (MQ=255)
                                 ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGTTc                                                                     <  1:3299158/101‑1 (MQ=255)
                                 ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTc                                                                     >  2:1164073/1‑101 (MQ=255)
                                 ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTc                                                                     <  1:4600122/101‑1 (MQ=255)
                                  cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCg                                                                    <  2:5568443/101‑1 (MQ=255)
                                  cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCg                                                                    <  2:4065760/101‑1 (MQ=255)
                                  cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTA.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCg                                                                    <  1:1338575/101‑1 (MQ=255)
                                    gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGTTCGCt                                                                  <  2:4473483/101‑1 (MQ=255)
                                    gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCt                                                                  >  1:1721609/1‑101 (MQ=255)
                                        cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccc                                                              >  1:2148448/1‑101 (MQ=255)
                                         tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                             <  2:3360357/101‑1 (MQ=255)
                                               gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggg                                                       >  2:5433233/1‑101 (MQ=255)
                                               gcggcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggg                                                       <  1:1460759/98‑1 (MQ=255)
                                                ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgggg                                                      >  1:4121894/1‑101 (MQ=255)
                                                 tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                     >  1:2128821/1‑101 (MQ=255)
                                                   ccACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCg                                                   >  1:231422/1‑101 (MQ=255)
                                                    cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCCCGGGGGCgg                                                  >  2:2074915/1‑100 (MQ=255)
                                                      cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGCTCCCCCCGCGGGGGCGTgg                                                >  2:1998169/1‑101 (MQ=255)
                                                       gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                               >  2:4722288/1‑101 (MQ=255)
                                                         tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:297145/101‑1 (MQ=255)
                                                         tGCCCTTGAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:3170683/101‑1 (MQ=255)
                                                         gaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTC..GACCGAG.TTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  2:2320606/92‑1 (MQ=0)
                                                            ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  1:2702821/101‑1 (MQ=255)
                                                            atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            >  1:2524685/7‑101 (MQ=2)
                                                            atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            >  2:595636/7‑101 (MQ=2)
                                                            atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            >  1:3680693/7‑101 (MQ=2)
                                                              ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGGGGGGGTCGAAAc                                        >  1:4424251/1‑101 (MQ=255)
                                                                gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACCg                                      >  1:1187353/1‑101 (MQ=255)
                                                                gAGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGT.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                      <  1:4029656/101‑1 (MQ=255)
                                                                 cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                       >  1:1051622/2‑100 (MQ=2)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGt                                    >  1:224627/1‑101 (MQ=255)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCCCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                     <  1:5176606/101‑4 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                    <  2:3927570/101‑5 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                 >  2:4448569/1‑101 (MQ=255)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                 <  2:5036019/101‑1 (MQ=255)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                 <  2:5270861/101‑1 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                  >  1:2441555/1‑95 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                <  1:5362230/101‑1 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                                 >  1:3800141/1‑94 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                                 <  1:4300806/101‑8 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                               <  1:2968348/101‑1 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                               >  2:1330017/1‑101 (MQ=255)
                                                                        ccccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                              <  2:1941883/101‑1 (MQ=255)
                                                                         cccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                             >  1:2387736/1‑101 (MQ=255)
                                                                         cccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                             <  1:5747245/101‑1 (MQ=255)
                                                                         cccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                             >  2:1617840/1‑101 (MQ=255)
                                                                          tcccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                            <  2:717364/100‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagaaga                            >  1:5320258/1‑98 (MQ=255)
                                                                           cccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                           >  1:717432/1‑101 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACCGTG.TGGAT...TATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                        <  2:2411135/101‑1 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                       >  2:3289115/1‑101 (MQ=255)
                                                                                gggggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                      <  1:956383/101‑1 (MQ=255)
                                                                                gggggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                      >  1:2843677/1‑101 (MQ=255)
                                                                                 ggggCGTGGATCGAAACCCGG.TGGAT...GATTATGCAGCGCGGGCGGCTTGGGTCGCTCCCCCCCCCGGGGGGGGGATCGAAAACCGGCCCGAGATAGAAcgc                     >  2:5760603/1‑101 (MQ=255)
                                                                                    gCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgcg                  >  1:1248913/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                >  1:5608522/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                >  1:3938777/1‑101 (MQ=255)
                                                                                          aTCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc            <  2:1233128/101‑1 (MQ=255)
                                                                                           tCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcc           <  1:3465768/101‑1 (MQ=255)
                                                                                            cGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct          <  2:1464889/101‑1 (MQ=255)
                                                                                               aaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg       >  1:2655490/1‑101 (MQ=255)
                                                                                               aaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg       >  1:3755478/1‑101 (MQ=255)
                                                                                                aCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa      >  2:1400297/1‑101 (MQ=255)
                                                                                                 ccGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc     <  1:4891823/101‑1 (MQ=255)
                                                                                                 ccGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc     >  2:2258552/1‑101 (MQ=255)
                                                                                                  cGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg    <  2:3512553/101‑1 (MQ=255)
                                                                                                  cGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg    <  1:1816929/101‑1 (MQ=255)
                                                                                                   gtg.tGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt   <  1:5795306/101‑1 (MQ=255)
                                                                                                    tg.tgGAT...GATCATGGAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc  <  2:3041374/101‑1 (MQ=255)
                                                                                                    tg.tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc  <  2:2868503/101‑1 (MQ=255)
                                                                                                    |                                                                                                        
CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTC  >  NC_014034/1396705‑1396905

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG