BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,956	T→G	11.6%	F280V (TTC→GAT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,956	0	T→G	11.6%	125.1 ‑0.3 ‑1.6	443	F280V (TTC→GTC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (26/21): ref base (185/170): total (233/210)
Fisher's exact test strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.25e-02

Only 100 of 482 total aligned reads displayed.


GTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396856‑1397056


gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.Ct                                                                                                           >  2:2107233/1‑101 (MQ=255)


  ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.Tc                                                                                                        >  2:3080472/1‑101 (MQ=255)


      cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGc.c                                                                                                   <  1:4569947/101‑1 (MQ=255)


        aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTg                                                                                                 >  2:3572558/1‑101 (MQ=255)


        aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTg                                                                                                 <  1:3935478/101‑1 (MQ=255)


         aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..c                                                                                              <  2:4452353/101‑1 (MQ=255)


          aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..Cg                                                                                             <  1:4402696/101‑1 (MQ=255)


                ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCg                                                                                      >  1:3979871/1‑101 (MQ=255)


                  agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCt                                                                                    >  1:5186631/1‑101 (MQ=255)


                      aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                                <  2:2318731/101‑1 (MQ=255)


                        aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                              <  2:1070310/101‑1 (MQ=255)


                          aCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCcgc                                                                            >  2:3474830/1‑101 (MQ=255)


                            cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                                          >  2:4808287/1‑101 (MQ=255)


                             gcgcgAACCTCCTCAGACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                         <  2:154493/101‑1 (MQ=255)


                              cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgggg                                                                        <  2:1777649/101‑1 (MQ=255)


                                 ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                     >  2:5211830/1‑101 (MQ=255)


                                    ctcgcggttgcGTCACTCCCCCCGCGGGGGCGTAGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                   <  1:4994562/91‑1 (MQ=0)


                                     tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  2:3646938/92‑1 (MQ=0)


                                     ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                 >  2:4527280/1‑101 (MQ=255)


                                      tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  1:2499455/101‑1 (MQ=255)


                                       tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               >  2:5744647/9‑101 (MQ=2)


                                       cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  2:2210262/101‑1 (MQ=255)


                                       cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  2:854770/101‑1 (MQ=255)


                                       cctcAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  1:2744148/101‑1 (MQ=255)


                                        ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:5312254/94‑1 (MQ=2)


                                        ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  2:5557210/94‑1 (MQ=2)


                                        ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  1:3719194/8‑101 (MQ=2)


                                        ggccccagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCCTATGCGC.TTGA.CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:3268649/94‑1 (MQ=2)


                                        ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GGCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  1:871400/1‑101 (MQ=255)


                                         tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  2:3938533/101‑1 (MQ=255)


                                         ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                >  2:5342359/6‑101 (MQ=2)


                                          cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               >  1:3092701/5‑101 (MQ=2)


                                          cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGGGGATc                                                               >  2:767479/5‑101 (MQ=0)


                                          ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            >  1:4669965/6‑101 (MQ=2)


                                          cccccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  2:500830/97‑1 (MQ=2)


                                          acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             >  1:493656/6‑101 (MQ=9)


                                          acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  1:481614/96‑1 (MQ=9)


                                          acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             >  2:946426/6‑101 (MQ=9)


                                           cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  2:4361005/97‑1 (MQ=2)


                                            gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          >  2:3265656/1‑101 (MQ=255)


                                            gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          >  1:296275/1‑101 (MQ=255)


                                            ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          >  1:2873016/4‑101 (MQ=2)


                                            actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           >  2:1388078/4‑101 (MQ=9)


                                             ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            >  1:1283973/2‑101 (MQ=2)


                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          <  2:120717/101‑1 (MQ=2)


                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccg                                                       <  1:1550236/101‑4 (MQ=2)


                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                      >  1:4289973/1‑101 (MQ=255)


                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCa                                                      >  2:4217307/1‑101 (MQ=255)


                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                       <  1:640744/101‑4 (MQ=9)


                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                        <  2:632506/101‑2 (MQ=2)


                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGGGGATGGaaaaac                                                        >  2:2966229/1‑98 (MQ=2)


                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                                     >  2:2508000/1‑101 (MQ=255)


                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                                     <  2:461384/101‑6 (MQ=2)


                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                      >  2:431342/1‑97 (MQ=9)


                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                      >  2:724523/1‑97 (MQ=9)


                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                     <  1:3627251/101‑6 (MQ=2)


                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGc                                                    >  1:1776266/1‑101 (MQ=255)


                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                    >  2:5157848/1‑95 (MQ=2)


                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                     >  1:1445904/1‑96 (MQ=9)


                                                   cTCCCCCCGGGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                                    <  2:2751965/101‑7 (MQ=2)


                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCa                                                      >  1:5382939/1‑101 (MQ=2)


                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                                    >  1:2210409/1‑95 (MQ=2)


                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                                   >  1:3513749/1‑94 (MQ=2)


                                                    tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                  >  2:1671867/1‑101 (MQ=255)


                                                    tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCtc                                                  >  2:4711993/1‑101 (MQ=255)


                                                    tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCG.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                  <  1:1685322/101‑1 (MQ=255)


                                                     ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                    <  1:22563/101‑3 (MQ=2)


                                                     ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                  <  2:2359298/101‑9 (MQ=9)


                                                     ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                  <  1:4154103/101‑9 (MQ=9)


                                                     ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                  <  2:4283306/101‑9 (MQ=9)


                                                      cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                                   <  2:5061901/101‑4 (MQ=2)


                                                      cccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgt                                                 <  1:1639134/101‑10 (MQ=2)


                                                      cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                                                >  1:182214/1‑101 (MQ=255)


                                                      cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCACTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                                                <  1:1988384/101‑1 (MQ=255)


                                                      cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                 <  1:212554/101‑10 (MQ=9)


                                                       ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                                  <  1:807733/101‑5 (MQ=2)


                                                        cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                                 >  1:1205958/1‑96 (MQ=0)


                                                         ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                                             <  2:3178568/101‑1 (MQ=255)


                                                          cgcgGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                               >  2:1969327/1‑94 (MQ=0)


                                                            cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGGCGTTgg                                          >  2:4873527/1‑101 (MQ=255)


                                                                   gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                   >  1:1152974/1‑101 (MQ=255)


                                                                    tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                  >  1:184949/1‑101 (MQ=255)


                                                                     ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGACAGCCGGa                                 <  2:1857742/101‑1 (MQ=255)


                                                                      gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                                <  1:2316727/101‑1 (MQ=255)


                                                                          gAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGA.CTG..CGTG.GTTGCCTCCCCCGCGGGGGCGTGAATCGAAACATCAGCTCGTCGTTTGGCAGCCGGACGATg                            >  2:3817537/1‑101 (MQ=255)


                                                                             aCA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                         >  1:4516402/1‑101 (MQ=255)


                                                                                 gCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                      <  1:1370919/101‑1 (MQ=255)


                                                                                     cgcgcG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                   <  1:4244751/100‑1 (MQ=255)


                                                                                      gcgcG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc                  >  1:4096206/1‑101 (MQ=255)


                                                                                        gcg.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc                <  2:2824837/101‑1 (MQ=255)


                                                                                            gC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc             <  2:3779935/101‑1 (MQ=255)


                                                                                            gC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc             <  2:1318875/101‑1 (MQ=255)


                                                                                                  a.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg        >  2:3351447/1‑101 (MQ=255)


                                                                                                  a.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg        >  1:5568686/1‑101 (MQ=255)


                                                                                                    tCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgggg       >  1:1014489/1‑101 (MQ=255)


                                                                                                      gg.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc     >  2:676001/1‑101 (MQ=255)


                                                                                                      gg.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCAAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc     <  2:4088154/101‑1 (MQ=255)


                                                                                                       g.CT.TCCGC.CTG..CGTG.GTCGCACCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCg    <  2:5687584/101‑1 (MQ=255)


                                                                                                          t.tCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTg  >  1:1041593/1‑101 (MQ=255)


                                                                                                          t.tCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGAGCGTg  >  1:5709576/1‑101 (MQ=255)


GTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396856‑1397056

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG