BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,014	T→A	17.0%	L299* (TTG→TAT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,014	0	T→A	17.0%	155.2 ‑0.6 ‑0.4	355	L299* (TTG→TAG)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (24/29): ref base (140/135): total (186/169)
Fisher's exact test strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.13e-01

Only 100 of 375 total aligned reads displayed.


CGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCG  >  NC_014034/1396914‑1397114


  cgGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.GCGTTgg                                                                                                         >  2:4873527/1‑101 (MQ=255)


   gggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGt                                                                                                        >  2:4581550/1‑101 (MQ=255)


    ggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTc                                                                                                       <  2:601408/101‑1 (MQ=255)


       gCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGATTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGc                                                                                                    >  2:5228674/1‑101 (MQ=255)


          tGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCgg                                                                                                 >  1:655194/1‑101 (MQ=255)


          tGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCgg                                                                                                 >  1:184949/1‑101 (MQ=255)


           ggATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TTGTTGGTGAACCggc                                                                                                >  1:1799396/1‑100 (MQ=38)


            gATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATGGAAAC.ATCAACTCG.TCGTTGGTCAGCCGG.Ac                                                                                              >  1:3614762/1‑101 (MQ=255)


            gATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.Ac                                                                                              <  1:2316727/101‑1 (MQ=255)


               cGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GAt                                                                                          >  1:2833236/1‑101 (MQ=255)


               cGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GAt                                                                                          >  1:3979368/1‑101 (MQ=255)


               cGAAACAGCCTGCGCGGCCGCACCGGCTTCCGCCTGCGTGGCCGCTCCCCCCGCGGGGGCGAGGGTCGAAAC.ATCAGCTCG.TCCTCGGACAGccctaccag                                                                                            >  2:289867/1‑94 (MQ=37)


                   aCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTcc                                                                                      >  2:2355122/1‑101 (MQ=255)


                   aCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTcc                                                                                      >  1:4516402/1‑101 (MQ=255)


                      gCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTc                                                                                <  2:4289713/101‑1 (MQ=255)


                      gCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTc                                                                                <  1:1370919/101‑1 (MQ=255)


                         cgcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCt                                                                             <  1:4244751/100‑1 (MQ=255)


                          gcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTc                                                                            >  1:4096206/1‑101 (MQ=255)


                          gcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTc                                                                            <  2:216078/101‑1 (MQ=255)


                            gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccc                                                                          <  2:2824837/101‑1 (MQ=255)


                            gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccc                                                                          <  1:5362718/101‑1 (MQ=255)


                            gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCAAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccc                                                                          <  1:1585644/101‑1 (MQ=255)


                              ggCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccccc                                                                        >  1:2288283/1‑101 (MQ=255)


                               gCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTcccccc                                                                       <  2:1318875/101‑1 (MQ=255)


                                  gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCcgcg                                                                    <  2:1036311/101‑1 (MQ=255)


                                  gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCcgcg                                                                    >  1:4150553/1‑101 (MQ=255)


                                    aTCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCggg                                                                  >  2:3351447/1‑101 (MQ=255)


                                    aTCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCggg                                                                  >  1:5568686/1‑101 (MQ=255)


                                       ggCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGc                                                               >  1:1668048/1‑101 (MQ=255)


                                       ggCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGc                                                               >  2:676001/1‑101 (MQ=255)


                                          ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                            >  1:1041593/1‑101 (MQ=255)


                                           tCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:1330105/101‑1 (MQ=255)


                                           tCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                           <  2:296242/101‑1 (MQ=255)


                                             gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:214744/92‑1 (MQ=2)


                                             gatcccgccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:3791461/92‑1 (MQ=2)


                                             cGCCTGGGTGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  2:4826537/101‑1 (MQ=255)


                                              gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        >  2:4005709/1‑101 (MQ=255)


                                              gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        >  1:5736583/1‑101 (MQ=255)


                                              gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:1250304/101‑1 (MQ=255)


                                              atcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                          >  1:1960896/9‑101 (MQ=2)


                                               ccagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         >  2:832349/9‑101 (MQ=2)


                                               ccagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         >  1:2946532/9‑101 (MQ=2)


                                                cccgccggTCGCTGTCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGCCCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:3856163/95‑1 (MQ=2)


                                                cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:1726980/95‑1 (MQ=2)


                                                cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:3425607/95‑1 (MQ=2)


                                                cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:3505401/95‑1 (MQ=2)


                                                cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:5105474/95‑1 (MQ=2)


                                                cagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        >  2:5778331/8‑101 (MQ=2)


                                                cagcggcgTCCCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:452088/94‑1 (MQ=2)


                                                 tGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  1:695705/1‑101 (MQ=255)


                                                 ccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       >  1:5223523/6‑101 (MQ=2)


                                                  cgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      <  1:1132375/97‑1 (MQ=2)


                                                   gggcttCGCTCCCCCCGCGGGGGCGTGGATCG.AAC.CCCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  2:1610554/96‑1 (MQ=2)


                                                   cGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  1:50238/101‑1 (MQ=255)


                                                    ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  2:122085/4‑101 (MQ=0)


                                                    gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                  >  1:3554694/1‑101 (MQ=255)


                                                    gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                  <  2:5483654/101‑1 (MQ=255)


                                                    gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                  <  1:3269946/101‑1 (MQ=255)


                                                    gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCCCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                  >  1:2452362/1‑101 (MQ=255)


                                                    gTGGTCGCTCCCCCCGCGGGGGCGTGG.TCGAAACAATCGGTTCG.CGGTTGTTAGGCGGG.AG.GAGGTCC...GTCCCTCCCCCCCCGGGGGGGGGGTTCGAACCt                                                  >  1:3179087/1‑101 (MQ=9)


                                                    ccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCC.CATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  1:5468170/99‑1 (MQ=2)


                                                     cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   >  2:4386957/2‑101 (MQ=2)


                                                     cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  1:1918638/100‑1 (MQ=2)


                                                      ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                  <  1:1452261/101‑2 (MQ=2)


                                                       gTCGCACCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcc                                               <  2:1078081/101‑1 (MQ=255)


                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                              <  1:1738514/101‑3 (MQ=2)


                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactg                                              >  1:4392982/1‑96 (MQ=2)


                                                            tCCCCCCGCGGGGGCGTTGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                            <  2:5146270/101‑5 (MQ=2)


                                                            tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                            >  2:1264101/1‑97 (MQ=2)


                                                            tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                          <  1:2717630/101‑1 (MQ=255)


                                                            tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                          >  2:2042296/1‑101 (MQ=255)


                                                            cccccccGCGGTGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCCCC...GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                            <  2:5703996/100‑5 (MQ=2)


                                                            cccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                          <  2:4150303/100‑1 (MQ=255)


                                                             ccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                                           <  2:4466407/101‑6 (MQ=2)


                                                                cccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                      <  2:5031921/101‑1 (MQ=255)


                                                                  cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGa                                    >  2:5307689/1‑101 (MQ=255)


                                                                  cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGa                                    >  1:2580869/1‑101 (MQ=255)


                                                                  cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGa                                    <  2:1956222/101‑1 (MQ=255)


                                                                  cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGCCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGCCtccattgt                                    >  1:4340170/1‑94 (MQ=37)


                                                                     gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                 >  2:1581755/1‑101 (MQ=255)


                                                                         gCGTGGCTCCAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                             <  2:2255135/101‑1 (MQ=255)


                                                                         gCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                             >  1:1000163/1‑101 (MQ=255)


                                                                         gCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                             <  2:268705/101‑1 (MQ=255)


                                                                              gATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                        <  2:4978754/101‑1 (MQ=255)


                                                                              gATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                        <  1:4712274/101‑1 (MQ=255)


                                                                              gATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                        <  2:179504/101‑1 (MQ=255)


                                                                                tCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                      <  1:1864331/101‑1 (MQ=255)


                                                                                tCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                      >  2:1649434/1‑101 (MQ=255)


                                                                                  gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                    <  1:4902285/101‑1 (MQ=255)


                                                                                   aaaC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                   <  2:2456357/101‑1 (MQ=255)


                                                                                    aaC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt                  <  2:3284342/101‑1 (MQ=255)


                                                                                    aaC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGGGATGGGGCGCGAGCACATgt                  >  2:4242268/1‑101 (MQ=255)


                                                                                         tCAGCTCG.TCGTTTGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgtc              >  2:8891/1‑101 (MQ=255)


                                                                                          cAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATctcgtcc             >  2:5620404/1‑100 (MQ=255)


                                                                                           aGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGc            >  2:5080520/1‑101 (MQ=255)


                                                                                           aGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGc            <  1:3351652/101‑1 (MQ=255)


                                                                                             cTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGAGCGAGCACATGTCGTCGCTc          >  2:375459/1‑101 (MQ=255)


                                                                                                    gtTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccccc    >  1:4608357/1‑100 (MQ=255)


                                                                                                     ttGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgc   <  1:4808568/101‑1 (MQ=255)


                                                                                                      tGGTCAGCCGT.AC.GATGGCC...TTCGCCCCCCCCGCGGTGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgcg  <  2:3614534/101‑1 (MQ=38)


CGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCG  >  NC_014034/1396914‑1397114

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG