Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,015 G→T 15.2% L299F (TTG→TAT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,0150G→T15.2% 137.7 ‑1.0 ‑0.0355L299F (TTG→TTT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (25/29):  ref base (161/140):  total (186/169)
Fisher's exact test strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 376 total aligned reads displayed.

GCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGG  >  NC_014034/1396915‑1397115
                                                                                                      |                                                                                                        
 cgGTGGCGTGGAGCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTgg                                                                                                         <  2:886436/101‑1 (MQ=255)
  gggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGt                                                                                                        >  2:5065095/1‑101 (MQ=255)
   ggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTc                                                                                                       >  2:5020080/1‑101 (MQ=255)
      gCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGc                                                                                                    >  2:10536/1‑101 (MQ=255)
         tGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCgg                                                                                                 >  1:184949/1‑101 (MQ=255)
          ggATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TTGTTGGTGAACCggc                                                                                                >  1:1799396/1‑100 (MQ=38)
           gATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAc                                                                                               <  1:2316727/101‑1 (MQ=255)
           gATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAc                                                                                               <  2:5357170/101‑1 (MQ=255)
            aTCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.g                                                                                             >  1:430438/1‑101 (MQ=255)
              cGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GAt                                                                                           >  1:2833236/1‑101 (MQ=255)
               gAAACAGCCTGCGCGGCCGCATCGGCTTCCGACTGCGTGGTTGCCTCCCCCGCGGGGGCGTGAATCGAAAC.ATCAGCTCG.TCGTTTGGCAGCCGGAC.GATg                                                                                          >  2:3817537/1‑101 (MQ=255)
                aaaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGt                                                                                         >  2:3612103/1‑101 (MQ=255)
                 aaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                                        <  2:4751067/101‑1 (MQ=255)
                    aGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...Gt                                                                                  <  2:1605084/101‑1 (MQ=255)
                     gCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTc                                                                                 <  1:1370919/101‑1 (MQ=255)
                        cgcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCt                                                                              <  1:4244751/100‑1 (MQ=255)
                         gcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTc                                                                             <  2:216078/101‑1 (MQ=255)
                         gcgcGGCCGCATCGGCTCCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTc                                                                             <  2:5194856/101‑1 (MQ=255)
                           gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccc                                                                           >  2:2329306/1‑101 (MQ=255)
                           gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCAAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccc                                                                           <  1:1585644/101‑1 (MQ=255)
                             ggCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccccc                                                                         >  1:2288283/1‑101 (MQ=255)
                             ggCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCC.TCGTTGGTCAGCCCGAC.GATGTCC...GCCACTccccc                                                                         >  1:4542785/1‑101 (MQ=38)
                             ggCCGCATCGGCTTCCGCCTGCGTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccccc                                                                         <  1:4135302/101‑1 (MQ=255)
                                cGCATCGGCTTCCGCCTGCGTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgc                                                                      <  1:674880/101‑1 (MQ=255)
                                 gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GACGCTCCCCCcgtg                                                                     >  1:4979035/1‑99 (MQ=255)
                                 gCATCCGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgcg                                                                     <  2:4333246/101‑1 (MQ=255)
                                    tCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCgggg                                                                  <  2:4190828/101‑1 (MQ=255)
                                      ggCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGc                                                                >  1:1668048/1‑101 (MQ=255)
                                      ggCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCAAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGc                                                                <  2:4088154/101‑1 (MQ=255)
                                         ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                             >  1:1041593/1‑101 (MQ=255)
                                         ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                             >  2:4410464/1‑101 (MQ=255)
                                         ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                             <  1:4496403/101‑1 (MQ=255)
                                         ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGAGCGTg                                                             >  1:5709576/1‑101 (MQ=255)
                                          tCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                            <  1:1330105/101‑1 (MQ=255)
                                            gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                            <  2:3335149/92‑1 (MQ=2)
                                            gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                            <  1:214744/92‑1 (MQ=2)
                                            gatcccgccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                            <  1:3791461/92‑1 (MQ=2)
                                             gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:1250304/101‑1 (MQ=255)
                                             atcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                           >  1:1960896/9‑101 (MQ=2)
                                              tcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                          >  2:5579222/8‑101 (MQ=2)
                                              ccagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                          >  1:2946532/9‑101 (MQ=2)
                                              ccTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  2:2843516/101‑1 (MQ=255)
                                               cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:1726980/95‑1 (MQ=2)
                                                 cgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                       <  1:1132375/97‑1 (MQ=2)
                                                   ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     >  2:5125078/4‑101 (MQ=0)
                                                   gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                   <  1:3269946/101‑1 (MQ=255)
                                                   gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCCCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                   >  1:2452362/1‑101 (MQ=255)
                                                   gTGGTCGCTCCCCCCGCGGGGGCGTGG.TCGAAACAATCGGTTCG.CGGTTGTTAGGCGGGAG.GAGGTCC...GTCCCTCCCCCCCCGGGGGGGGGGTTCGAACCt                                                   >  1:3179087/1‑101 (MQ=9)
                                                    cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    <  1:1918638/100‑1 (MQ=2)
                                                     ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGGGGGGATGGAAAcg                                                   >  1:4066345/1‑100 (MQ=2)
                                                     ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                   <  1:1452261/101‑2 (MQ=2)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcc                                                >  2:1785528/1‑101 (MQ=255)
                                                        ccccccccccGCGGTGGCGTTGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                              <  2:793454/97‑1 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcg                                                >  1:5202010/1‑100 (MQ=2)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                              >  2:2757552/1‑101 (MQ=255)
                                                         ggaagccccGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                               >  2:292523/6‑99 (MQ=2)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                               <  1:1738514/101‑3 (MQ=2)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactg                                               >  2:3744135/1‑96 (MQ=2)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactga                                              >  1:4361271/1‑95 (MQ=2)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                             >  2:59120/1‑97 (MQ=2)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaa                                             >  2:259648/1‑97 (MQ=2)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                           >  2:2042296/1‑101 (MQ=255)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                           <  1:2717630/101‑1 (MQ=255)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactgac                                             >  1:5043634/1‑94 (MQ=2)
                                                            ccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCCGTCTTg                                          >  2:1893058/1‑101 (MQ=255)
                                                             cccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGa                                         <  2:3456013/101‑1 (MQ=255)
                                                               cccGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatct                                         <  2:3899382/101‑9 (MQ=2)
                                                               cccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                       <  1:3568337/101‑1 (MQ=255)
                                                               cccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                       <  2:4571202/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGa                                     >  1:2580869/1‑101 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTAAGCCGGAC.GATGTCC...GGCGGTCGCCCCGCGGTGGCGGGGCTCGGAACGGACGTGtttcggagtg                                   >  2:644472/1‑93 (MQ=255)
                                                                    gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                  <  1:579763/101‑1 (MQ=255)
                                                                    gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                  >  1:3361209/1‑101 (MQ=255)
                                                                    gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCA...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                  >  2:243757/1‑101 (MQ=255)
                                                                       ggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgc                               <  1:3951260/101‑1 (MQ=255)
                                                                        gCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                              >  1:1000163/1‑101 (MQ=255)
                                                                        gCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                              <  2:1384779/101‑1 (MQ=255)
                                                                          gTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                            >  2:2233359/1‑101 (MQ=255)
                                                                          gTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                            >  2:5281748/1‑101 (MQ=255)
                                                                            ggATCGAAAC.ATCAGCTNG.TCGTGGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                          <  2:1259883/101‑1 (MQ=255)
                                                                            ggATCGAAAC.ATCAGCTCG.TCGTTGGTTAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGGGATGGGGCGCGAg                          >  1:5391774/1‑101 (MQ=255)
                                                                              aTCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcgggggcc                        >  1:4661654/1‑95 (MQ=255)
                                                                               tCGAAAC.GTCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                       <  1:5557506/101‑1 (MQ=255)
                                                                               tCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                       <  1:1864331/101‑1 (MQ=255)
                                                                               tCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                       >  2:1649434/1‑101 (MQ=255)
                                                                                 gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                     <  2:4516130/101‑1 (MQ=255)
                                                                                 gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                     <  2:4286966/101‑1 (MQ=255)
                                                                                 gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                     >  1:5267650/1‑101 (MQ=255)
                                                                                   aaC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt                   <  1:3504596/101‑1 (MQ=255)
                                                                                     c.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg                 >  2:3262754/1‑101 (MQ=255)
                                                                                       aTCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGGGCGGGCACATgtcgt                >  1:3424881/1‑101 (MQ=255)
                                                                                       aTCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgt                >  2:1140062/1‑101 (MQ=255)
                                                                                           gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt            <  2:4852460/101‑1 (MQ=255)
                                                                                             tcg.tcgTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcc          >  2:130420/1‑101 (MQ=255)
                                                                                             tcg.tcgTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcc          >  2:5693523/1‑101 (MQ=255)
                                                                                              cg.tcgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccc         >  1:4791343/1‑101 (MQ=255)
                                                                                                  cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc      <  2:5736288/101‑1 (MQ=255)
                                                                                                   tttGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcg     <  1:4845584/100‑1 (MQ=255)
                                                                                                   gtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcg     <  1:989087/101‑1 (MQ=255)
                                                                                                    ttGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgc    <  2:4958906/101‑1 (MQ=255)
                                                                                                      |                                                                                                        
GCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGG  >  NC_014034/1396915‑1397115

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG