BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,016	G→C	16.6%	V300L (GTC→CTA)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,016	0	G→C	16.6%	133.3 ‑0.6 ‑0.7	355	V300L (GTC→CTC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (24/29): ref base (140/136): total (186/169)
Fisher's exact test strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.12e-01

Only 100 of 378 total aligned reads displayed.


CGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGG  >  NC_014034/1396916‑1397116


cgGTGGCGTGGAGCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTgg                                                                                                           <  2:886436/101‑1 (MQ=255)


cgGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTgg                                                                                                           >  2:4348316/1‑101 (MQ=255)


cgGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.GCGTTgg                                                                                                           >  2:4873527/1‑101 (MQ=255)


  ggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTc                                                                                                         >  2:5020080/1‑101 (MQ=255)


  ggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTc                                                                                                         <  1:470214/101‑1 (MQ=255)


   gggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCa                                                                                                        >  2:3984824/1‑101 (MQ=255)


     gCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGc                                                                                                      >  2:10536/1‑101 (MQ=255)


     gCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGATTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGc                                                                                                      >  2:5228674/1‑101 (MQ=255)


        tGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCgg                                                                                                   >  1:184949/1‑101 (MQ=255)


         ggATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TTGTTGGTGAACCggc                                                                                                  >  1:1799396/1‑100 (MQ=38)


             cGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GAt                                                                                            >  1:3979368/1‑101 (MQ=255)


              gAAACAGCCTGCGCGGCCGCATCGGCTTCCGACTGCGTGGTTGCCTCCCCCGCGGGGGCGTGAATCGAAAC.ATCAGCTCG.TCGTTTGGCAGCCGG.AC.GATg                                                                                           >  2:3817537/1‑101 (MQ=255)


               aaaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGt                                                                                          >  2:3612103/1‑101 (MQ=255)


                   aGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...Gt                                                                                   <  2:1605084/101‑1 (MQ=255)


                    gCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTc                                                                                  <  1:1370919/101‑1 (MQ=255)


                       cgcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCt                                                                               <  1:4244751/100‑1 (MQ=255)


                        gcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTc                                                                              <  2:216078/101‑1 (MQ=255)


                        gcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTc                                                                              >  1:4096206/1‑101 (MQ=255)


                          gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccc                                                                            >  2:2329306/1‑101 (MQ=255)


                          gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCAAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccc                                                                            <  1:1585644/101‑1 (MQ=255)


                           cgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTcccc                                                                           >  1:2456491/1‑101 (MQ=255)


                            ggCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccccc                                                                          >  1:2288283/1‑101 (MQ=255)


                            ggCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCC.TCGTTGGTCAGCCCG.AC.GATGTCC...GCCACTccccc                                                                          >  1:4542785/1‑101 (MQ=38)


                            ggCCGCATCGGCTTCCGCCTGCGTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTccccc                                                                          <  1:4135302/101‑1 (MQ=255)


                                gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCcgcg                                                                      <  2:1036311/101‑1 (MQ=255)


                                gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GACGCTCCCCCcgtg                                                                      >  1:4979035/1‑99 (MQ=255)


                                   tCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCgggg                                                                   <  2:4190828/101‑1 (MQ=255)


                                     ggCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGc                                                                 >  1:1668048/1‑101 (MQ=255)


                                        ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                              >  1:1041593/1‑101 (MQ=255)


                                         tCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:1330105/101‑1 (MQ=255)


                                          ccGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  1:5213436/101‑1 (MQ=255)


                                           gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:214744/92‑1 (MQ=2)


                                           gatcccgccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:3791461/92‑1 (MQ=2)


                                           cGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           >  2:3504379/1‑101 (MQ=255)


                                            gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:1250304/101‑1 (MQ=255)


                                            atcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                            >  1:1960896/9‑101 (MQ=2)


                                             tcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           >  2:5579222/8‑101 (MQ=2)


                                             tcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  1:2983761/94‑1 (MQ=2)


                                              cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:3425607/95‑1 (MQ=2)


                                              cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  2:1726980/95‑1 (MQ=2)


                                               tGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  1:695705/1‑101 (MQ=255)


                                                cgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        <  1:1132375/97‑1 (MQ=2)


                                                  gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                    >  1:3554694/1‑101 (MQ=255)


                                                  gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                    <  1:3269946/101‑1 (MQ=255)


                                                  gTGGTCGCTCCCCCCGCGGGGGCGTGG.TCGAAACAATCGGTTCG.CGGTTGTTAGGCGGG.AG.GAGGTCC...GTCCCTCCCCCCCCGGGGGGGGGGTTCGAACCt                                                    >  1:3179087/1‑101 (MQ=9)


                                                  ccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCC.CATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  1:5468170/99‑1 (MQ=2)


                                                   cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  1:4804389/100‑1 (MQ=2)


                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTNGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                    >  1:1749097/1‑100 (MQ=2)


                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                    <  1:1452261/101‑2 (MQ=2)


                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                  >  2:3335395/1‑101 (MQ=255)


                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGc                                                  >  2:5075782/1‑101 (MQ=2)


                                                       ccccccccccGCGGTGGCGTTGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                               <  2:793454/97‑1 (MQ=255)


                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                               <  1:5065379/101‑1 (MQ=255)


                                                        ggaagccccGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                                >  2:292523/6‑99 (MQ=2)


                                                        gcgccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTc                                              <  1:5269020/98‑1 (MQ=255)


                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactg                                                >  1:4392982/1‑96 (MQ=2)


                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTG.CC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactg                                                >  2:3744135/1‑96 (MQ=2)


                                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgta                                               <  1:2886316/101‑4 (MQ=2)


                                                          tCCCCCCGCGGGGGCGTTGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                              <  2:5146270/101‑5 (MQ=2)


                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                              >  2:59120/1‑97 (MQ=2)


                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaa                                              >  2:259648/1‑97 (MQ=2)


                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                            >  2:2042296/1‑101 (MQ=255)


                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                            <  1:2717630/101‑1 (MQ=255)


                                                          cccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                            <  2:4150303/100‑1 (MQ=255)


                                                           ccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGG.TCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                                             <  2:4466407/101‑6 (MQ=2)


                                                           ccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCCGTCTTg                                           >  2:1893058/1‑101 (MQ=255)


                                                              cccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                        <  1:3568337/101‑1 (MQ=255)


                                                              cccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                        <  2:4571202/101‑1 (MQ=255)


                                                                cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGa                                      >  1:2580869/1‑101 (MQ=255)


                                                                cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGCCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGCCtccattgt                                      >  1:4340170/1‑94 (MQ=37)


                                                                  cgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTAAGCCGG.AC.GATGTCC...GGCGGTCGCCCCGCGGTGGCGGGGCTCGGAACGGACGTGtttcggagtg                                    >  2:644472/1‑93 (MQ=255)


                                                                   gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                   >  1:3361209/1‑101 (MQ=255)


                                                                   gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCA...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                   >  2:243757/1‑101 (MQ=255)


                                                                      ggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgc                                <  1:605713/101‑1 (MQ=255)


                                                                       gCGTGGCTCCAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                               <  2:2255135/101‑1 (MQ=255)


                                                                       gCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                               >  1:1000163/1‑101 (MQ=255)


                                                                       gCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                               <  2:1384779/101‑1 (MQ=255)


                                                                         gTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                             >  2:5281748/1‑101 (MQ=255)


                                                                          tGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa                            >  1:5564240/1‑101 (MQ=255)


                                                                           ggATCGAAAC.ATCAGCTNG.TCGTGGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                           <  2:1259883/101‑1 (MQ=255)


                                                                           ggATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGac                           >  2:4787265/1‑100 (MQ=255)


                                                                            gATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                          <  2:4978754/101‑1 (MQ=255)


                                                                            gATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                          <  2:179504/101‑1 (MQ=255)


                                                                              tCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                        <  1:1864331/101‑1 (MQ=255)


                                                                              tCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                        >  2:1649434/1‑101 (MQ=255)


                                                                                gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                      <  2:4286966/101‑1 (MQ=255)


                                                                                gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                      <  2:4516130/101‑1 (MQ=255)


                                                                                   aC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc                   >  2:2865085/1‑101 (MQ=255)


                                                                                    c.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg                  >  2:3262754/1‑101 (MQ=255)


                                                                                      aTCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgt                 >  2:1140062/1‑101 (MQ=255)


                                                                                       tCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgtc                <  1:5723666/101‑1 (MQ=255)


                                                                                        cAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGtcgtcg               <  1:1922372/101‑1 (MQ=255)


                                                                                          gCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGGACATGTCGTCGCt             >  1:4860515/1‑101 (MQ=255)


                                                                                            tcg.tcgTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGGGAGCACATGTCGTCGCTcc           >  2:5445910/1‑101 (MQ=255)


                                                                                            tcg.tcgTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcc           >  2:5693523/1‑101 (MQ=255)


                                                                                            tcg.tcgTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcc           >  2:130420/1‑101 (MQ=255)


                                                                                                 cgtTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc       <  2:5736288/101‑1 (MQ=255)


                                                                                                 cgtTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc       <  1:2355256/101‑1 (MQ=255)


                                                                                                 cgtTGGTCAGCCGG.AC.GATGTCC...GCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGCCTTGAGTGATGGGGGGCGAGCACATGTCGCCGCGcccccc       >  2:2814755/1‑101 (MQ=38)


                                                                                                     ggTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCgg   <  1:4502765/101‑1 (MQ=255)


CGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGG.AC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGG  >  NC_014034/1396916‑1397116

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG