BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,134	C→A	4.2%	S339Y (TCC→TAA)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,134	0	C→A	4.2%	17.3 ‑2.8 ‑0.5	264	S339Y (TCC→TAC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (2/9): ref base (128/124): total (131/133)
Fisher's exact test strand distribution p-value = 6.01e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.26e-01

Only 100 of 292 total aligned reads displayed.


GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCG  >  NC_014034/1397034‑1397234


gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTc                                                                                                       <  2:854432/101‑1 (MQ=255)


gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTc                                                                                                       <  2:3361004/101‑1 (MQ=255)


     tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                                                                                                  >  2:4294974/1‑101 (MQ=255)


     tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                                                                                                  <  1:3333370/101‑1 (MQ=255)


     tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                                                                                                  >  1:1100904/1‑101 (MQ=255)


      ccccccGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTc                                                                                                 <  2:2696294/101‑1 (MQ=255)


      ccccccGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTc                                                                                                 <  2:1602733/101‑1 (MQ=255)


      ccccccGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTc                                                                                                 <  1:5307979/101‑1 (MQ=255)


         cccGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTcgcg                                                                                              <  1:2237822/101‑1 (MQ=255)


           cgcgGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGcc                                                                                            >  2:3604870/1‑101 (MQ=255)


            gcgGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCG.ACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGCGCGAAACGGccgggagatgc                                                                                             >  2:581498/1‑92 (MQ=38)


               ggggCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                        <  2:1460571/101‑1 (MQ=255)


               ggggCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                        >  2:5114314/1‑101 (MQ=255)


               ggggCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                        <  2:3578605/101‑1 (MQ=255)


                gggCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATg                                                                                       >  2:1720550/1‑101 (MQ=255)


                   cGTGGATCTAAACTGCCGTCT.TTAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                    <  1:2757713/101‑1 (MQ=255)


                   cGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                    <  2:2387602/101‑1 (MQ=255)


                    gTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                   >  2:2433880/1‑101 (MQ=255)


                    gTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                   <  2:5148201/101‑1 (MQ=255)


                    gTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                   <  2:5623705/101‑1 (MQ=255)


                    gTGGATCGAAACTGCCGGCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTACCCCCGCGGGGGCGTGGATCGAAACGTACAGCTCCTGCCACATGTCtt                                                                                   >  1:5778358/1‑101 (MQ=38)


                     tGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                  >  2:5294679/1‑101 (MQ=255)


                     tGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                  >  1:804164/1‑101 (MQ=255)


                     tGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                  >  2:1968625/1‑101 (MQ=255)


                      ggATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                 >  1:5545293/1‑101 (MQ=255)


                      ggATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                 >  1:930451/1‑101 (MQ=255)


                       gATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                                <  2:3765122/101‑1 (MQ=255)


                        aTCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAc                                                                               >  2:380837/1‑101 (MQ=255)


                          cGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                            <  1:1671966/101‑1 (MQ=255)


                          cGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCCCGCGCCCCATGTATTCCACC.g                                                                            >  1:3854972/1‑101 (MQ=38)


                           gAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.Gt                                                                           <  1:2432111/101‑1 (MQ=255)


                           gAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.Gt                                                                           <  2:778048/101‑1 (MQ=255)


                            aaaCTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTg                                                                          >  2:5392432/1‑101 (MQ=255)


                             aaCTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgc                                                                         <  2:4116716/101‑1 (MQ=255)


                                   cGTCT.TGAGTGATGGGGCGCGAGCC.CATGTC.GTCGATCCCCCCGCGGGCGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTc                                                                   <  1:4744722/101‑1 (MQ=255)


                                      cT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCt                                                                >  2:5443413/1‑101 (MQ=255)


                                      cT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GGGCGCCGTCGcc                                                                >  2:4705124/1‑100 (MQ=255)


                                       t.tGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                               >  1:4793348/1‑101 (MQ=255)


                                        tgcgatGATGGGGCGCGTGCT.CGTGTC.GTCGGTCCCCACGAGGGGGCGCCGATCGAAAGGTCCAGCTTGCGGCACATGTCTTCGGCC.GTGCGCCGTCGCTc                                                               <  2:4542513/96‑1 (MQ=255)


                                         tGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                              >  2:74441/1‑101 (MQ=255)


                                         tGAGTGATGGGGCGCGAGAA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                              >  2:2217423/1‑101 (MQ=255)


                                           aGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                            >  1:2928425/1‑101 (MQ=255)


                                             tGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                          <  1:5726106/101‑1 (MQ=255)


                                             tGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                          >  1:3154092/1‑101 (MQ=255)


                                             tGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAACTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                          <  1:2042413/101‑1 (MQ=255)


                                              gATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                         >  1:5169680/1‑101 (MQ=255)


                                              gATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                         <  2:5773229/101‑1 (MQ=255)


                                               aTGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgc                                                        >  2:2786131/1‑101 (MQ=255)


                                               aTGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTCCGCCGTCGCTCCCCCcgc                                                        >  1:4549036/1‑101 (MQ=255)


                                                tGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgcg                                                       <  2:184929/101‑1 (MQ=255)


                                                     cgcgAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                  <  1:766066/101‑1 (MQ=255)


                                                       ggAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGt                                                <  2:2193607/100‑1 (MQ=255)


                                                        gAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                               >  2:2848340/1‑101 (MQ=255)


                                                         aGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                              <  1:1785627/101‑1 (MQ=255)


                                                         aGCA.CATGTC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                              <  1:1968736/101‑1 (MQ=255)


                                                           gcgcaTGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  1:4273248/4‑101 (MQ=255)


                                                           ca.caTGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            >  2:4992340/1‑101 (MQ=255)


                                                             cggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:694555/93‑1 (MQ=0)


                                                              caTGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  1:2102726/1‑101 (MQ=255)


                                                              caTGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  2:122367/1‑101 (MQ=255)


                                                                tGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:3572775/101‑1 (MQ=255)


                                                                 tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:5488032/97‑1 (MQ=2)


                                                                 tgcggtcgCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  2:3471743/97‑1 (MQ=2)


                                                                 gtc.gtcGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  1:265886/1‑101 (MQ=255)


                                                                  tc.gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      <  1:1389436/101‑1 (MQ=255)


                                                                  tc.ggcgCTCCCCCCTCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCGCATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      <  2:3854730/101‑1 (MQ=255)


                                                                   cggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      >  2:3055741/3‑101 (MQ=2)


                                                                   c.gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                     >  2:4598578/1‑101 (MQ=255)


                                                                     gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                    <  2:799304/101‑3 (MQ=2)


                                                                      tcgCTCCCGCCGCGGGGGCGTGGATCGAAAAGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtg                                   <  2:4791062/101‑1 (MQ=255)


                                                                      tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtg                                   >  1:5035652/1‑101 (MQ=255)


                                                                       cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                  >  1:4217262/1‑101 (MQ=255)


                                                                       cgCGCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                  <  2:1860693/101‑1 (MQ=255)


                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                 <  2:4650309/101‑1 (MQ=255)


                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                <  2:1100829/101‑1 (MQ=255)


                                                                          tCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                               <  1:2560812/101‑1 (MQ=255)


                                                                          cccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                               <  1:5227843/100‑1 (MQ=255)


                                                                           ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                              <  2:1027012/101‑1 (MQ=255)


                                                                              cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                           <  2:2580727/101‑1 (MQ=255)


                                                                              cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                           >  1:3212384/1‑101 (MQ=255)


                                                                                cgcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacga                         >  1:2584201/1‑101 (MQ=255)


                                                                                   gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                      >  2:5720392/1‑101 (MQ=255)


                                                                                    ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGcc                     >  2:3718165/1‑101 (MQ=255)


                                                                                     gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGccc                    <  1:4354509/101‑1 (MQ=255)


                                                                                     gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GGGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGccc                    <  1:4868778/101‑1 (MQ=255)


                                                                                       gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCtc                  >  2:1014547/1‑100 (MQ=255)


                                                                                       gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTg                  >  1:1602351/1‑101 (MQ=255)


                                                                                       gCGTGGATCGAAACGTCCAGCTCGCGCCACATGGCTTTGACC.GTGCGCCGTCGCTCCTCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTg                  <  1:4950485/101‑1 (MQ=255)


                                                                                        cGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGc                 >  2:3951898/1‑101 (MQ=255)


                                                                                           ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc              >  1:4459181/1‑101 (MQ=255)


                                                                                           ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc              <  1:3817779/101‑1 (MQ=255)


                                                                                            gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa             <  2:1832246/101‑1 (MQ=255)


                                                                                            gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa             >  2:1919553/1‑101 (MQ=255)


                                                                                            gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCACGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa             <  1:2383024/101‑1 (MQ=255)


                                                                                               cGAAACGTCCAGCTCGCGCCACATGTCTTCGCCC.GTGCGCCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt          <  1:3945448/101‑1 (MQ=255)


                                                                                               cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt          >  1:5452925/1‑101 (MQ=255)


                                                                                                gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc         >  1:1211689/1‑101 (MQ=255)


                                                                                                   aCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcgc      <  2:1360882/101‑1 (MQ=255)


                                                                                                       ccAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCg  <  2:3194425/101‑1 (MQ=255)


                                                                                                       ccAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCg  <  2:2079765/101‑1 (MQ=255)


GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCT.TGAGTGATGGGGCGCGAGCA.CATGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCG  >  NC_014034/1397034‑1397234

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG