Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,162 G→C 4.9% V348V (GTG→GCC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1620G→C 4.9% 19.9 ‑4.0 ‑0.8246V348V (GTG→GTC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/10):  ref base (122/112):  total (124/122)
Fisher's exact test strand distribution p-value = 1.87e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.54e-01

Only 100 of 269 total aligned reads displayed.

AAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1397062‑1397262
                                                                                                     |                                                                                                    
aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTg                                                                                                      <  2:1877888/101‑1 (MQ=255)
aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGGCTTCGACC.GTg                                                                                                      >  2:2963111/1‑101 (MQ=255)
    tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGcc                                                                                                  <  1:243790/101‑1 (MQ=255)
       cGTCTTGAGTGATGGGGCGCGAGCCCATGTCGTCGATCCCCCCGCGGGCGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTc                                                                                               <  1:4744722/101‑1 (MQ=255)
         tCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGc                                                                                             >  2:1951694/1‑101 (MQ=255)
          cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCt                                                                                            >  1:5205190/1‑101 (MQ=255)
          cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCt                                                                                            >  2:2417990/1‑101 (MQ=255)
            tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                                                          <  1:5446200/101‑1 (MQ=255)
            tGAGTGATGGGGCGCGAGAACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                                                          >  2:2217423/1‑101 (MQ=255)
             gAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccc                                                                                         >  1:3172693/1‑101 (MQ=255)
              aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                                                        >  1:2928425/1‑101 (MQ=255)
               gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                                       <  2:655137/101‑1 (MQ=255)
                tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                                                      >  1:3154092/1‑101 (MQ=255)
                 gATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                                     >  1:5169680/1‑101 (MQ=255)
                 gATGGGGCGCGAGCACATGTCGACGCCGCCCCCGGGGGGGCGTGGATCGAAACGGCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                                     <  2:1268944/101‑1 (MQ=38)
                    ggggCGCGAGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                                  <  1:5620696/101‑1 (MQ=255)
                          cgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCgg                                                                            >  2:2612428/1‑100 (MQ=255)
                          cgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCgg                                                                            >  1:2624732/1‑100 (MQ=255)
                           gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                           >  2:2189371/1‑101 (MQ=255)
                            aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  2:4182114/101‑1 (MQ=255)
                            aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  2:1667949/101‑1 (MQ=255)
                            aGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  1:1968736/101‑1 (MQ=255)
                             gcgcaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  1:4273248/4‑101 (MQ=255)
                              cacaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  2:4992340/1‑101 (MQ=255)
                                gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:2830031/95‑1 (MQ=0)
                                caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  2:122367/1‑101 (MQ=255)
                                caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  1:2102726/1‑101 (MQ=255)
                                 ctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  2:1851869/96‑1 (MQ=2)
                                 ctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  2:184872/96‑1 (MQ=2)
                                  tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:4563999/97‑1 (MQ=2)
                                  tgcggtcgCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  2:3471743/97‑1 (MQ=2)
                                  tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  1:5457366/1‑101 (MQ=255)
                                  tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:3572775/101‑1 (MQ=255)
                                   gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  1:5265673/1‑101 (MQ=255)
                                   gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  2:3150741/1‑101 (MQ=255)
                                   gcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  2:405911/4‑101 (MQ=2)
                                    tcggcgCTCCCCCCTCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCGCATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  <  2:3854730/101‑1 (MQ=255)
                                     cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                                 <  1:2013977/101‑1 (MQ=255)
                                      gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCGACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                                <  2:3595092/101‑3 (MQ=2)
                                      gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                                <  2:799304/101‑3 (MQ=2)
                                        cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                                              >  1:4217262/1‑101 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                             <  2:4650309/101‑1 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                             <  1:1649528/101‑1 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTATTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                             <  2:5674838/101‑1 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                                            >  1:5073593/1‑101 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                                            <  1:3612331/101‑1 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                                            <  2:1100829/101‑1 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                                          <  2:1027012/101‑1 (MQ=255)
                                             cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                                                         >  1:238926/1‑101 (MQ=255)
                                              ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                                        <  2:5267360/101‑1 (MQ=255)
                                              ccccGCGGGGGCGGGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                                        <  2:4774115/101‑1 (MQ=255)
                                               cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                                                       >  2:3724799/1‑101 (MQ=255)
                                                 cgcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacga                                                     >  1:3902269/1‑101 (MQ=255)
                                                 cgcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacga                                                     >  1:2584201/1‑101 (MQ=255)
                                                   ggggggCGTGGATCGAAACGTCCAGCTCGCGCCGCATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgacg                                                   <  2:4608081/100‑1 (MQ=255)
                                                    gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGGCACGACGc                                                  >  2:507415/1‑101 (MQ=255)
                                                    gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                                                  >  1:1876171/1‑101 (MQ=255)
                                                      gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGccc                                                >  2:1832088/1‑101 (MQ=255)
                                                        gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCtc                                              >  2:1014547/1‑100 (MQ=255)
                                                        gCGTGGATCGAAACGTCCAGCTCGCGCCACATGGCTTTGACC.GTGCGCCGTCGCTCCTCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTg                                              <  1:4950485/101‑1 (MQ=255)
                                                          gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa                                            >  1:4853859/1‑101 (MQ=255)
                                                           tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAc                                           >  1:1417566/1‑101 (MQ=255)
                                                            ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc                                          >  1:4459181/1‑101 (MQ=255)
                                                            ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc                                          <  1:3817779/101‑1 (MQ=255)
                                                              aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg                                        >  1:2328556/1‑101 (MQ=255)
                                                                cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.TTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt                                      <  2:5364307/101‑1 (MQ=255)
                                                                 gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc                                     <  2:4518032/101‑1 (MQ=255)
                                                                  aaaCGTCCAGCTCGCCACACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc                                    <  2:4548764/101‑1 (MQ=255)
                                                                       tCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGcc                               <  2:5775296/101‑1 (MQ=255)
                                                                       tCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGTCTGCCACGGCGCCCCGCACCAGATCCGCGcc                               >  2:5427405/1‑101 (MQ=255)
                                                                        ccAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCg                              <  2:3194425/101‑1 (MQ=255)
                                                                           gCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCg                           >  1:2793831/1‑101 (MQ=255)
                                                                           gCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCg                           >  2:5741348/1‑101 (MQ=255)
                                                                            cTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGc                          >  2:3523304/1‑101 (MQ=255)
                                                                             tCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCt                         <  2:840817/101‑1 (MQ=255)
                                                                               gcgcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTcc                       <  2:3979627/101‑1 (MQ=255)
                                                                               gcgcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTcc                       <  2:4095959/101‑1 (MQ=255)
                                                                                cgcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTccc                      <  1:4787546/101‑1 (MQ=255)
                                                                                cgcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTccc                      >  1:490501/1‑101 (MQ=255)
                                                                                  ccACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTccccc                    >  1:3951528/1‑101 (MQ=255)
                                                                                    acaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcg                  <  1:126510/101‑1 (MQ=255)
                                                                                    acaTGGCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCATGGCGCCCTGCGCCAGATCTGCGCCGTCGCTCCCCCcg                  >  2:3809118/1‑101 (MQ=37)
                                                                                      aTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgcg                >  2:4342463/1‑101 (MQ=255)
                                                                                      aTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgcg                >  2:4919901/1‑101 (MQ=255)
                                                                                      aTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGCCGCTCCCCCcgcg                >  1:7571/1‑101 (MQ=255)
                                                                                       tGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgg               >  2:551424/1‑101 (MQ=255)
                                                                                        gTCTTCGGCC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg              <  2:2807003/101‑1 (MQ=255)
                                                                                        gTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg              <  2:3370865/101‑1 (MQ=255)
                                                                                        gTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg              <  1:5799115/101‑1 (MQ=255)
                                                                                         tCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgggg             >  1:3409905/1‑101 (MQ=255)
                                                                                          cTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg            <  2:5241892/101‑1 (MQ=255)
                                                                                          cTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg            <  2:1467776/101‑1 (MQ=255)
                                                                                          cTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg            >  1:1328427/1‑101 (MQ=255)
                                                                                          cTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg            <  1:1026069/101‑1 (MQ=255)
                                                                                            tCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCg          >  2:169523/1‑101 (MQ=255)
                                                                                            tCGACC.GCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTTCCCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCg          <  1:809066/101‑1 (MQ=255)
                                                                                              gACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg        <  1:4342729/101‑1 (MQ=255)
                                                                                               gcc.GTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTgg       <  2:213247/100‑1 (MQ=255)
                                                                                               aCC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGGGTgg       >  2:2740712/1‑101 (MQ=255)
                                                                                                 c.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt     <  1:5764582/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
AAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1397062‑1397262

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG