Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,223 A→T 1.8% R369* (AGA→TGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2230A→T 1.8% 5.4 ‑1.6 ‑0.1271R369* (AGA→TGA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (140/125):  total (141/130)
Fisher's exact test strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.43e-01

Only 100 of 291 total aligned reads displayed.

GATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTG  >  NC_014034/1397123‑1397323
                                                                                                      |                                                                                                    
 aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAg                                                                                                     >  1:2328556/1‑101 (MQ=255)
 aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAg                                                                                                     <  2:1932465/101‑1 (MQ=255)
   cGAAACGTCCAGCTCGCGCCACATGTCTTCTACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGAt                                                                                                   <  1:2612577/101‑1 (MQ=255)
    gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGACAGAAACGTGTCTGCCA.CGACG.CACTGCACCCGaca                                                                                                  >  2:1689792/1‑99 (MQ=38)
     aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATcc                                                                                                 >  1:1285726/1‑101 (MQ=255)
       aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCcgc                                                                                               <  2:1360882/101‑1 (MQ=255)
          tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGcc                                                                                            <  2:5775296/101‑1 (MQ=255)
            cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGt                                                                                          <  2:3297716/101‑1 (MQ=255)
              gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCg                                                                                        >  1:2793831/1‑101 (MQ=255)
                  gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTcc                                                                                    >  2:3560924/1‑101 (MQ=255)
                  gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTcc                                                                                    <  2:182194/101‑1 (MQ=255)
                   cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTccc                                                                                   >  1:490501/1‑101 (MQ=255)
                   cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTccc                                                                                   >  2:3677812/1‑101 (MQ=255)
                     ccccaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTccccc                                                                                 <  2:1253076/98‑1 (MQ=255)
                     ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTccccc                                                                                 >  1:3951528/1‑101 (MQ=255)
                       acaTGGCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.TGGCG.CCCTGCGCCAGATCTGCGCCGTCGCTCCCCCcg                                                                               >  2:3809118/1‑101 (MQ=37)
                         aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGCCGCTCCCCCcgcg                                                                             >  1:7571/1‑101 (MQ=255)
                          tGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgg                                                                            >  2:551424/1‑101 (MQ=255)
                            tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgggg                                                                          >  1:3409905/1‑101 (MQ=255)
                             cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg                                                                         >  1:1956111/1‑101 (MQ=255)
                             cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg                                                                         <  2:5241892/101‑1 (MQ=255)
                              ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGc                                                                        >  1:5206309/1‑101 (MQ=255)
                               tccgtggTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCg                                                                       <  2:1149719/95‑1 (MQ=255)
                                 gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                     >  2:218013/1‑101 (MQ=255)
                                  gccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  1:2865246/100‑1 (MQ=255)
                                    cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  >  2:4103890/1‑101 (MQ=255)
                                    cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  >  2:4046397/1‑101 (MQ=255)
                                    cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:4543519/101‑1 (MQ=255)
                                     gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  1:5080805/101‑1 (MQ=255)
                                       ctatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGA.CCTGGTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  1:2658345/97‑1 (MQ=2)
                                        cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCA.ACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               <  1:5732615/98‑1 (MQ=2)
                                         gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  2:5035369/101‑1 (MQ=255)
                                         gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  1:998230/101‑1 (MQ=255)
                                         gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  1:5263044/101‑1 (MQ=255)
                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                           >  2:3432715/1‑100 (MQ=255)
                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCA.ACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                            <  1:1168617/101‑1 (MQ=2)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                         >  2:20135/1‑101 (MQ=255)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                         >  1:1442161/1‑101 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                        <  2:3951284/101‑1 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                        >  2:3086845/1‑101 (MQ=255)
                                              cGCTCACCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.AGACG.CCCTGCAACAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                        <  2:1876061/101‑1 (MQ=255)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                       >  1:4320863/1‑101 (MQ=255)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                                      >  1:3323721/1‑101 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAACCGTGTCTGCCA.CGACC.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                                    <  1:5443703/101‑1 (MQ=255)
                                                   cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                                   >  1:4372834/1‑101 (MQ=255)
                                                     cccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgc                                                 >  2:2905157/1‑101 (MQ=255)
                                                      ccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcccc                                                >  1:219753/1‑98 (MQ=255)
                                                       cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGTCG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                               <  1:4992621/101‑1 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgcgc                                             >  2:4594879/1‑100 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                             >  1:4734736/1‑101 (MQ=255)
                                                          gggggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                            >  2:4348960/1‑101 (MQ=255)
                                                           ggggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                           <  2:2564609/101‑1 (MQ=255)
                                                           ggggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                           >  2:5729503/1‑101 (MQ=255)
                                                            gggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                          <  2:2102609/101‑1 (MQ=255)
                                                            gggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                          >  2:4700103/1‑101 (MQ=255)
                                                             ggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCGCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                         >  1:386068/1‑101 (MQ=255)
                                                             ggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                         >  2:831434/1‑101 (MQ=255)
                                                             ggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                         >  1:2123283/1‑101 (MQ=255)
                                                                 tGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                                     >  2:4482854/1‑101 (MQ=255)
                                                                  ggATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                    <  2:1442068/101‑1 (MQ=255)
                                                                  ggATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTAGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                    >  2:5008388/1‑101 (MQ=255)
                                                                   gATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAg                                   >  1:1375859/1‑101 (MQ=255)
                                                                    aTCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                                  >  1:3689442/1‑101 (MQ=255)
                                                                      cGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                                >  1:5682347/1‑101 (MQ=255)
                                                                      cGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                                <  1:3016944/101‑1 (MQ=255)
                                                                       gAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                               >  2:3140949/1‑101 (MQ=255)
                                                                         aaCGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgc                             >  1:3248233/1‑101 (MQ=255)
                                                                          aCGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                            >  1:5797042/1‑101 (MQ=255)
                                                                            gtgtCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                          >  1:4583628/1‑101 (MQ=255)
                                                                            gtgtCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                          >  1:1320114/1‑101 (MQ=255)
                                                                             tgtCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                         <  1:1832198/101‑1 (MQ=255)
                                                                              gtCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                        >  2:4209994/1‑101 (MQ=255)
                                                                                cTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                      <  1:1707013/101‑1 (MQ=255)
                                                                                cTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                      <  2:3845361/101‑1 (MQ=255)
                                                                                cTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                      <  1:4490147/101‑1 (MQ=255)
                                                                                 tGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTcc                     >  1:1027131/1‑100 (MQ=255)
                                                                                 tGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                     <  1:1919662/101‑1 (MQ=255)
                                                                                 tGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                     <  2:5129377/101‑1 (MQ=255)
                                                                                  gCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc                    >  2:3175961/1‑101 (MQ=255)
                                                                                    cA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc                  <  1:20145/101‑1 (MQ=255)
                                                                                     a.cgacg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcc                 >  1:5131508/1‑101 (MQ=255)
                                                                                       cgacg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccc                <  1:3523521/101‑1 (MQ=255)
                                                                                        gacg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc               <  1:1224789/101‑1 (MQ=255)
                                                                                         acg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc              <  1:1014622/101‑1 (MQ=255)
                                                                                          cg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc             >  2:4803271/1‑101 (MQ=255)
                                                                                           g.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg            <  2:1724190/101‑1 (MQ=255)
                                                                                           g.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg            >  2:3503487/1‑101 (MQ=255)
                                                                                           g.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCCCGAAAGAAGCTCCCCCcg            >  2:5344343/1‑101 (MQ=37)
                                                                                             cccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgc           <  2:2328430/101‑1 (MQ=255)
                                                                                              ccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgcg          <  2:1526534/101‑1 (MQ=255)
                                                                                              ccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgcg          >  1:2420866/1‑101 (MQ=255)
                                                                                                 gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa       >  1:383077/1‑101 (MQ=255)
                                                                                                  cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg      <  2:5545001/101‑1 (MQ=255)
                                                                                                  cACCAGATCCGCGCCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg      <  2:2795182/101‑1 (MQ=255)
                                                                                                   aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCTGCGGGGGc     >  2:2713559/1‑101 (MQ=255)
                                                                                                   aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc     <  1:816565/101‑1 (MQ=255)
                                                                                                     cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgcagatcgga   >  2:2257142/1‑93 (MQ=255)
                                                                                                     cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt   >  1:5517509/1‑101 (MQ=255)
                                                                                                     cAGACCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt   <  2:5410438/101‑1 (MQ=255)
                                                                                                      aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg  <  2:1211606/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
GATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTG  >  NC_014034/1397123‑1397323

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG