Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,231 G→A 1.9% A371A (GCG→ACA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2310G→A 1.9% 3.9 ‑1.7 ‑0.1267A371A (GCG→GCA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (140/122):  total (141/126)
Fisher's exact test strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.81e-01

Only 100 of 291 total aligned reads displayed.

CGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAA  >  NC_014034/1397131‑1397331
                                                                                                     |                                                                                                    
cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                                                      >  1:4437727/1‑101 (MQ=255)
  tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                                    <  2:5775296/101‑1 (MQ=255)
  tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGTCTGCCA.CGGCGCCCCGCACCAGATCCGCGcc                                                                                                    >  2:5427405/1‑101 (MQ=255)
   ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCg                                                                                                   >  2:1549675/1‑101 (MQ=255)
   ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCg                                                                                                   >  2:4619573/1‑101 (MQ=255)
      gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCg                                                                                                >  1:2793831/1‑101 (MQ=255)
        tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCt                                                                                              >  2:3450100/1‑101 (MQ=255)
         cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTc                                                                                             <  1:3725027/101‑1 (MQ=255)
          gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcc                                                                                            <  2:1805535/101‑1 (MQ=255)
          gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcc                                                                                            <  2:3979627/101‑1 (MQ=255)
           cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccc                                                                                           <  2:5265383/101‑1 (MQ=255)
           cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccc                                                                                           >  2:3677812/1‑101 (MQ=255)
               acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcg                                                                                       <  1:126510/101‑1 (MQ=255)
               acaTGGCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.TGGCGCCCTGCGCCAGATCTGCGCCGTCGCTCCCCCcg                                                                                       >  2:3809118/1‑101 (MQ=37)
                 aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgcg                                                                                     >  2:4919901/1‑101 (MQ=255)
                 aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgcg                                                                                     >  2:4062058/1‑101 (MQ=255)
                 aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGCCGCTCCCCCcgcg                                                                                     >  1:7571/1‑101 (MQ=255)
                   gTCTTCGGCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg                                                                                   <  2:2807003/101‑1 (MQ=255)
                       tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGCCGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCg                                                                               >  1:3454148/1‑101 (MQ=255)
                         gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                             <  2:5169394/101‑1 (MQ=255)
                         gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                             <  1:4342729/101‑1 (MQ=255)
                          gccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                            <  1:2865246/100‑1 (MQ=255)
                          gccGTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                            <  2:213247/100‑1 (MQ=255)
                          aCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGGGTgg                                                                            >  2:2740712/1‑101 (MQ=255)
                            cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                          <  2:4543519/101‑1 (MQ=255)
                               gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                       <  2:4272988/101‑1 (MQ=255)
                                 gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     >  1:1842094/1‑101 (MQ=255)
                                  ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                    <  2:4116134/101‑1 (MQ=255)
                                  ccGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                    <  1:5032495/101‑1 (MQ=255)
                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                    <  1:1168617/101‑1 (MQ=2)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                  >  2:4418445/1‑101 (MQ=255)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                                 <  2:1328339/101‑1 (MQ=255)
                                      cGCTCACCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.AGACGCCCTGCAACAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                                <  2:1876061/101‑1 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                               >  2:5060436/1‑101 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACGTGGCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                                              >  1:5129663/1‑101 (MQ=255)
                                           cgcccGGGGGGGGGTGAATCGAAACGTGTCTGCCA.CGCCCCCCTGCACCAGATCCCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                                           <  2:1233854/99‑1 (MQ=25)
                                              ccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcg                                                        <  1:130440/101‑1 (MQ=255)
                                                  gggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                                    >  1:3554904/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                                    >  2:4348960/1‑101 (MQ=255)
                                                   ggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGTACCAGATCCGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                                   <  1:4952432/101‑1 (MQ=255)
                                                   ggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                                   >  2:5729503/1‑101 (MQ=255)
                                                    gggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                                  <  1:5720687/101‑1 (MQ=255)
                                                    gggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                                  <  1:3939043/101‑1 (MQ=255)
                                                    gggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                                  <  1:3259251/101‑1 (MQ=255)
                                                     ggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                                 >  2:831434/1‑101 (MQ=255)
                                                       cGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                               <  2:5524653/101‑1 (MQ=255)
                                                        gTGGATCGAAACGTGTCTGCCC.CGTCGCGCTGCACCAGATCCGCGCTGTCACTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                                              <  2:5391484/101‑1 (MQ=37)
                                                        gTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                                              <  2:2928258/101‑1 (MQ=255)
                                                        gTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                                              >  2:2187488/1‑101 (MQ=255)
                                                         tGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                                             >  2:5026508/1‑101 (MQ=255)
                                                         tGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                                             >  1:2649917/1‑101 (MQ=255)
                                                          ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                            <  2:1442068/101‑1 (MQ=255)
                                                           gATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAg                                           <  1:2020312/101‑1 (MQ=255)
                                                            aTCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                                          >  1:5253518/1‑101 (MQ=255)
                                                            aTCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                                          >  2:2265733/1‑101 (MQ=255)
                                                              cGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                                        <  1:3016944/101‑1 (MQ=255)
                                                              cGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                                        >  1:384344/1‑101 (MQ=255)
                                                               gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                                       >  2:3325284/1‑101 (MQ=255)
                                                               gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                                       <  1:5294969/101‑1 (MQ=255)
                                                               gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                                       >  2:3088320/1‑101 (MQ=255)
                                                                  aCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                                    >  1:5797042/1‑101 (MQ=255)
                                                                   cGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGa                                   <  2:315738/101‑1 (MQ=255)
                                                                    gtgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                                  <  2:2624583/101‑1 (MQ=255)
                                                                      gtCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                                >  2:3515338/1‑101 (MQ=255)
                                                                        cTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                              <  1:4705405/101‑1 (MQ=255)
                                                                        cTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                              <  2:3845361/101‑1 (MQ=255)
                                                                         tGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTcc                             >  1:1027131/1‑100 (MQ=255)
                                                                          gCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc                            >  1:5609395/1‑101 (MQ=255)
                                                                          gCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc                            >  1:4281199/1‑101 (MQ=255)
                                                                           ccA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt                           <  1:142539/101‑1 (MQ=255)
                                                                            cA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc                          <  1:1920848/101‑1 (MQ=255)
                                                                             a.cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcc                         >  2:1198968/1‑101 (MQ=255)
                                                                             a.cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcc                         >  1:4776015/1‑101 (MQ=255)
                                                                             a.cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcc                         <  2:2348352/101‑1 (MQ=255)
                                                                                gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc                       <  1:919952/101‑1 (MQ=255)
                                                                                 acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc                      <  1:1014622/101‑1 (MQ=255)
                                                                                  cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc                     <  1:1549769/101‑1 (MQ=255)
                                                                                   gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg                    <  2:1724190/101‑1 (MQ=255)
                                                                                   gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg                    <  1:2132644/101‑1 (MQ=255)
                                                                                    tccgatctcAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgc                   <  1:2257268/93‑1 (MQ=255)
                                                                                    cccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgc                   >  1:133033/1‑101 (MQ=255)
                                                                                      cTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCgg                 >  1:5194971/1‑101 (MQ=255)
                                                                                      cTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGCCGCTCCCCCCGCgg                 >  2:3604598/1‑101 (MQ=255)
                                                                                       tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg                <  2:4734455/101‑1 (MQ=255)
                                                                                       cgCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg                <  2:2078122/100‑1 (MQ=255)
                                                                                        gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa               <  2:1976878/101‑1 (MQ=255)
                                                                                         cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg              >  2:1482843/1‑101 (MQ=255)
                                                                                          aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc             <  1:816565/101‑1 (MQ=255)
                                                                                          aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc             >  1:3328207/1‑101 (MQ=255)
                                                                                            cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt           >  1:5517509/1‑101 (MQ=255)
                                                                                             aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg          <  1:1971094/101‑1 (MQ=255)
                                                                                              gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGAgg         >  2:2549532/1‑101 (MQ=255)
                                                                                               aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGa        <  2:5555097/101‑1 (MQ=255)
                                                                                                tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGAt       >  1:2674540/1‑101 (MQ=255)
                                                                                                tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCCCGGGAGCGTGGAt       >  1:1737972/1‑101 (MQ=255)
                                                                                                 ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc      <  2:1064734/101‑1 (MQ=255)
                                                                                                  cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg     <  1:456753/101‑1 (MQ=255)
                                                                                                   gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa    >  1:3212310/1‑101 (MQ=255)
                                                                                                    cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa   <  1:2549674/101‑1 (MQ=255)
                                                                                                    cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa   <  2:3248033/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAA  >  NC_014034/1397131‑1397331

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG