Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,534 C→T 4.8% N472N (AAC→TAT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5340C→T 4.8% 28.9 0.0 ‑0.1320N472N (AAC→AATRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/8):  ref base (137/161):  total (147/173)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.86e-01

Only 100 of 367 total aligned reads displayed.

GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGG  >  NC_014034/1397434‑1397633
                                                                                                    |                                                                                                         
gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaac                                                                                                           <  1:5404421/101‑1 (MQ=255)
  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                                                                                         <  1:5773307/101‑1 (MQ=255)
   cccccccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCg                                                                                                        <  1:1199049/98‑1 (MQ=255)
    cTCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGa                                                                                                       <  2:2855344/101‑1 (MQ=255)
        ccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                                                                   <  2:3172504/101‑1 (MQ=255)
         cccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                                                                  <  1:667877/101‑1 (MQ=255)
         cccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                                                                  <  1:4831626/101‑1 (MQ=255)
          ccGCGGGGGCGTGGATCGAAACACCGTCATTACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                                                                                 <  1:5250688/101‑1 (MQ=255)
          ccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                                                                                 <  1:5040128/101‑1 (MQ=255)
          ccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                                                                                 <  2:4853578/101‑1 (MQ=255)
          ccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                                                                                 >  1:268016/1‑101 (MQ=255)
           cgcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                                                                                <  2:2945543/101‑1 (MQ=255)
            gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc.c                                                                                              <  2:3512484/101‑1 (MQ=255)
            cttccgatctGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc.c                                                                                              <  2:5761822/92‑1 (MQ=255)
             cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc.c..c                                                                                           <  1:4080749/101‑1 (MQ=255)
              gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..Cg                                                                                          >  2:556348/1‑101 (MQ=255)
              gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..Cg                                                                                          >  1:4137189/1‑101 (MQ=255)
                  gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                      >  2:2178349/1‑101 (MQ=255)
                   cGTGGATCCAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCa                                                                                     <  1:3395240/101‑1 (MQ=255)
                     tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgc                                                                                   <  1:5565396/101‑1 (MQ=255)
                     tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgc                                                                                   <  2:4335193/101‑1 (MQ=255)
                      ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                  <  1:1385918/101‑1 (MQ=255)
                         tCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgccg                                                                               >  2:1021237/1‑101 (MQ=255)
                          cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCCGcgccg.c                                                                             >  2:3957486/1‑101 (MQ=255)
                            aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCg.cgc                                                                           >  2:339084/1‑101 (MQ=255)
                             aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCt                                                                          >  2:4691473/1‑101 (MQ=255)
                             aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCt                                                                          <  1:236997/101‑1 (MQ=255)
                              acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.g                                                                        <  1:1104576/101‑1 (MQ=255)
                               cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.Ga                                                                       >  2:3625373/1‑101 (MQ=255)
                                acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.c                                                                     >  2:2523140/1‑101 (MQ=255)
                                 ccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.Cg                                                                    >  1:3803664/1‑101 (MQ=255)
                                    tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCg                                                                 >  1:836117/1‑101 (MQ=255)
                                      atcaCCACGCAACCGCCCTGCACCCCCGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCt                                                               <  1:1481508/101‑1 (MQ=255)
                                      atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCt                                                               <  1:3822898/101‑1 (MQ=255)
                                       tcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTc                                                              <  2:2056374/101‑1 (MQ=255)
                                       tcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTc                                                              <  1:2169665/101‑1 (MQ=255)
                                         accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTccc                                                            <  1:3321922/101‑1 (MQ=255)
                                            acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTcccccc                                                         >  2:2313764/1‑101 (MQ=255)
                                               cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCcgcg                                                      <  1:4773674/101‑1 (MQ=255)
                                                aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCgg                                                     >  2:5380486/1‑101 (MQ=255)
                                                aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCgg                                                     >  2:5043873/1‑101 (MQ=255)
                                                aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCCCCCCCCGCgg                                                     >  2:4718922/1‑101 (MQ=255)
                                                 aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGGGCCG.CGCT.GG.CGTCGCTCCCCCCGCggg                                                    >  2:1932552/1‑101 (MQ=255)
                                                    gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                 >  1:796788/1‑101 (MQ=255)
                                                       cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                              <  2:127250/101‑1 (MQ=255)
                                                       cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                              <  2:3712684/101‑1 (MQ=255)
                                                        ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                            <  1:1010202/91‑1 (MQ=2)
                                                          caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:1679812/101‑1 (MQ=255)
                                                             cgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCG.GGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  2:421171/97‑1 (MQ=0)
                                                             cgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCG.GGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  1:2242879/97‑1 (MQ=0)
                                                              accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       >  1:1779768/1‑101 (MQ=255)
                                                                cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     >  1:516151/1‑101 (MQ=255)
                                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCGCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                    <  1:929925/101‑1 (MQ=255)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                   >  2:1663266/1‑101 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGGGGGGAGCGaaaagggg                                 >  1:3578633/1‑96 (MQ=0)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                   <  1:4196028/101‑2 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                  <  2:731929/101‑1 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGGTCGaaaaggggc                                >  1:4491237/1‑95 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                 <  2:137027/101‑2 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                 <  1:1586032/101‑2 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                 >  1:2507597/1‑100 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                  >  2:1224800/1‑99 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                  <  1:2082591/101‑3 (MQ=2)
                                                                    cGCCCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                  <  2:5119525/101‑3 (MQ=0)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                               <  1:2812404/101‑7 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                               <  1:5590525/101‑7 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                >  2:3816304/1‑99 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                 <  2:4993897/101‑4 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                 <  2:4518772/101‑4 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGAGGCGTGGATCGAAACaaaa                                 <  2:3157138/101‑4 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                >  1:2574482/1‑101 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATGCC.G..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                               <  2:3322691/101‑4 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                               <  1:3055915/101‑4 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaac                                >  1:749210/1‑97 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                >  1:2312889/1‑97 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                             <  2:1792929/101‑9 (MQ=2)
                                                                         cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                            >  1:433869/1‑95 (MQ=2)
                                                                         cccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                             <  2:5250879/101‑8 (MQ=0)
                                                                          ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                           <  2:4136939/101‑1 (MQ=255)
                                                                                gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                     >  2:4101642/1‑101 (MQ=255)
                                                                                   ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                  >  1:2903989/1‑101 (MQ=255)
                                                                                    gCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                 <  2:3090147/101‑1 (MQ=255)
                                                                                     cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                >  2:2660237/1‑101 (MQ=255)
                                                                                     cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                <  1:3666405/101‑1 (MQ=255)
                                                                                      ggggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg               <  2:1492902/99‑1 (MQ=255)
                                                                                      gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg               >  2:819288/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg               >  1:1265107/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg               <  2:1714259/101‑1 (MQ=255)
                                                                                       tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc              >  1:4624084/1‑101 (MQ=255)
                                                                                       tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc              >  2:5476236/1‑101 (MQ=255)
                                                                                       tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc              >  2:3841246/1‑101 (MQ=255)
                                                                                        ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGGcccgcc             >  1:1991030/1‑101 (MQ=255)
                                                                                        agATCGAAACAACCGGATCATGCC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCTGATCGGCGTGAcccgcc             <  2:3275296/100‑1 (MQ=255)
                                                                                         gATCGAAACAACCCGATCGTTTC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc            <  2:670665/101‑1 (MQ=255)
                                                                                           tCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg          >  2:4626226/1‑101 (MQ=255)
                                                                                              aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc       >  1:371742/1‑101 (MQ=255)
                                                                                              aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc       >  1:3850196/1‑101 (MQ=255)
                                                                                                acaacCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt     >  2:1541827/1‑101 (MQ=255)
                                                                                                 caacCCGATCATGGC.C..CGCCTGAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc    >  1:3278616/1‑101 (MQ=255)
                                                                                                   acCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg  >  2:2436555/1‑101 (MQ=255)
                                                                                                    |                                                                                                         
GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGG  >  NC_014034/1397434‑1397633

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG