BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,537	G→T	13.7%	P473P (CCG→AAT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,537	0	G→T	13.7%	112.6 ‑1.1 ‑0.0	337	P473P (CCG→CCT)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (18/28): ref base (139/151): total (157/180)
Fisher's exact test strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.55e-01

Only 100 of 362 total aligned reads displayed.


GCTCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGC  >  NC_014034/1397437‑1397637


cccccccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCg                                                                                                             <  1:1199049/98‑1 (MQ=255)


   ccccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATc                                                                                                          <  1:5595589/101‑1 (MQ=255)


   ccccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATc                                                                                                          >  1:3847344/1‑101 (MQ=255)


   ccccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATc                                                                                                          >  1:3712912/1‑101 (MQ=255)


    cccccTCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCa                                                                                                         <  2:1266363/101‑1 (MQ=255)


    cccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCa                                                                                                         >  1:3822330/1‑101 (MQ=255)


      cccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATg                                                                                                       <  2:1769706/101‑1 (MQ=255)


      cccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGACCATg                                                                                                       >  1:4420752/1‑101 (MQ=255)


         gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c                                                                                                   <  1:1256653/101‑1 (MQ=255)


         gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c                                                                                                   <  2:3512484/101‑1 (MQ=255)


         gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c                                                                                                   >  1:3202834/1‑101 (MQ=255)


          cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c..c                                                                                                <  1:5223908/101‑1 (MQ=255)


          cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c..c                                                                                                >  2:5324094/1‑101 (MQ=255)


            ggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGc                                                                                              <  2:3999705/101‑1 (MQ=255)


             gggCGTGGATGGAAACACCGTCATCACCACGCAACCGCCCTGCACCGGCGGTAGGTCACCCCGCGGGGGCGTGGATCGAAACAAA.CCGATCATGGC.C..CGcc                                                                                             >  2:4809728/1‑101 (MQ=37)


             gggCGTGGATCGAAACCCCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGcc                                                                                             <  2:5794635/101‑1 (MQ=255)


                   ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.Agcg                                                                                      <  1:1385918/101‑1 (MQ=255)


                   ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.Agcg                                                                                      >  2:2391789/1‑101 (MQ=255)


                       cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.CGcgccg.c                                                                                 >  2:3957486/1‑101 (MQ=255)


                         aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCg.cgc                                                                               >  2:339084/1‑101 (MQ=255)


                          aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCt                                                                              <  1:236997/101‑1 (MQ=255)


                           acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.g                                                                            <  1:1104576/101‑1 (MQ=255)


                            cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.Ga                                                                           >  1:2859937/1‑101 (MQ=255)


                            cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.Ga                                                                           <  2:263783/101‑1 (MQ=255)


                             cccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.c                                                                         <  1:3801799/100‑1 (MQ=255)


                                gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTc                                                                      >  1:4180537/1‑101 (MQ=255)


                                 tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCg                                                                     <  2:2475402/101‑1 (MQ=255)


                                   atcaCCACGCAACCGCCCTGCACCCCCGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCt                                                                   <  1:1481508/101‑1 (MQ=255)


                                   atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCt                                                                   >  1:2607650/1‑101 (MQ=255)


                                     cactacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTcc                                                                 >  2:4322439/1‑101 (MQ=255)


                                      accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTccc                                                                <  1:3321922/101‑1 (MQ=255)


                                          cGCAACCGCCCTGCACCCCCGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCcg                                                            <  2:1346350/101‑1 (MQ=255)


                                                 gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                     >  1:796788/1‑101 (MQ=255)


                                                    cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                  <  2:3712684/101‑1 (MQ=255)


                                                     ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTT.CACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  1:1010202/91‑1 (MQ=2)


                                                      gCACCACCGGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACAAC.CCAAAAATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  1:4810009/101‑1 (MQ=38)


                                                      gCACCACCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  2:5194685/101‑1 (MQ=255)


                                                       caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                               <  1:1679812/101‑1 (MQ=255)


                                                         tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  2:5621194/95‑1 (MQ=0)


                                                         ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  1:2222844/101‑1 (MQ=255)


                                                          cgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAAT.CG.GCCG.GGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                            <  2:421171/97‑1 (MQ=0)


                                                          cgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  1:3618269/96‑1 (MQ=0)


                                                          accgcgTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCGATGATCAAATC.C..GCAATC.GGCCGGG.CTCG.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  1:1553335/96‑1 (MQ=0)


                                                           cccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  2:4689789/100‑1 (MQ=255)


                                                             cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                         >  1:5588896/1‑101 (MQ=255)


                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTT.CACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcggg                                      >  2:4707655/1‑98 (MQ=2)


                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                       <  1:4666627/101‑1 (MQ=2)


                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                       >  2:1055317/1‑101 (MQ=255)


                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCC.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                       <  1:531319/101‑1 (MQ=255)


                                                               gTCGCACCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATC.C..GCAATC.GGCCGGG.CTCG.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                        <  1:4623812/101‑2 (MQ=2)


                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                      <  1:338403/101‑1 (MQ=2)


                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                      >  1:1904913/1‑101 (MQ=255)


                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                      <  1:3035318/101‑3 (MQ=2)


                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                      <  1:2082591/101‑3 (MQ=2)


                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                      >  2:1495369/1‑99 (MQ=2)


                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                     <  1:886857/101‑1 (MQ=255)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTT.CACGGCG.GGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggcg                                   >  2:2311462/1‑95 (MQ=0)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                    >  2:3816304/1‑99 (MQ=2)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                     >  2:1553241/1‑98 (MQ=2)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                    >  2:3074379/1‑101 (MQ=255)


                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCGATGCC.G..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                   <  2:3322691/101‑4 (MQ=2)


                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgat                                   >  2:3565456/1‑98 (MQ=2)


                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaac                                    >  1:749210/1‑97 (MQ=2)


                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                    <  2:5069185/101‑5 (MQ=2)


                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                    >  1:2312889/1‑97 (MQ=2)


                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                   <  1:4905232/101‑1 (MQ=255)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCT.GT.C..GGCCGCGAACACCG.ATAC.CG.CGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgttc                                  >  2:2835365/1‑97 (MQ=2)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                  >  1:5073642/1‑101 (MQ=255)


                                                                    cccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                  <  2:5013856/100‑5 (MQ=2)


                                                                     ccccccGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTT.CACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                <  2:3833086/101‑10 (MQ=2)


                                                                     ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGCCTTGGACCGAAACATCCGCgg                                 >  2:313427/1‑101 (MQ=255)


                                                                      cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                >  1:3913391/1‑95 (MQ=2)


                                                                      cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                <  1:3565673/101‑7 (MQ=2)


                                                                       ccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                               <  1:2780411/101‑1 (MQ=255)


                                                                       ccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                               <  2:1969322/101‑1 (MQ=255)


                                                                        cccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                              <  2:550667/101‑1 (MQ=255)


                                                                           gcgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                           <  1:1745408/101‑1 (MQ=255)


                                                                             gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                         >  2:5447002/1‑101 (MQ=255)


                                                                              ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                        >  1:543436/1‑101 (MQ=255)


                                                                                 gCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                     <  2:4431657/101‑1 (MQ=255)


                                                                                  cGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                    >  2:1898290/1‑101 (MQ=255)


                                                                                   gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                   <  2:4953732/101‑1 (MQ=255)


                                                                                   gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                   <  2:1398028/101‑1 (MQ=255)


                                                                                    tGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                  >  1:4111341/1‑101 (MQ=255)


                                                                                    tGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                  >  2:4110810/1‑101 (MQ=255)


                                                                                     ggATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccccc                 >  2:723943/1‑101 (MQ=255)


                                                                                     agATCGAAACAAC.CGGATCATGCC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCTGATCGGCGTGAcccgcc                 <  2:3275296/100‑1 (MQ=255)


                                                                                      gATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                >  2:2158615/1‑101 (MQ=255)


                                                                                       ctCGAAACAAC.CCGGTCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc               <  2:2874002/100‑1 (MQ=255)


                                                                                       aTCGAATCAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc               <  1:973383/101‑1 (MQ=255)


                                                                                       aTCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc               <  1:2158737/101‑1 (MQ=255)


                                                                                        tCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg              >  2:4626226/1‑101 (MQ=255)


                                                                                           aaaCAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCGCCGACCGGCGTGACCcgccccgccc           >  2:3159004/1‑101 (MQ=255)


                                                                                           aaaCAAC.CAGAGCATGGC.C..CCCCTC.AGCGCCG.CACG.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc           <  1:644530/101‑1 (MQ=37)


                                                                                            aacaac.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa          >  2:1680940/1‑101 (MQ=255)


                                                                                             acacc.CCGATCAGGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt         <  1:2523282/101‑1 (MQ=255)


                                                                                             acaac.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgcccccccccc         >  1:4264022/1‑99 (MQ=255)


                                                                                                ac.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg      <  2:734497/101‑1 (MQ=255)


                                                                                                    cGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg   >  2:2663564/1‑101 (MQ=255)


                                                                                                    cGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAGCGTCGTGGCACGCCACGCCCCTCGGtag   >  2:888457/1‑99 (MQ=25)


GCTCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGC  >  NC_014034/1397437‑1397637

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG