BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,538	A→G	13.9%	M474V (ATC→GCC)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,538	0	A→G	13.9%	113.8 ‑1.1 ‑0.4	338	M474V (ATC→GTC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (19/28): ref base (140/151): total (159/179)
Fisher's exact test strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.98e-01

Only 100 of 366 total aligned reads displayed.


CTCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCT  >  NC_014034/1397438‑1397638


    ccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCAt                                                                                                        <  2:3172504/101‑1 (MQ=255)


     cccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATg                                                                                                       <  1:4831626/101‑1 (MQ=255)


      ccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATgg                                                                                                      <  1:5040128/101‑1 (MQ=255)


        gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c                                                                                                   <  1:1256653/101‑1 (MQ=255)


        gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c                                                                                                   <  2:3512484/101‑1 (MQ=255)


         cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c..c                                                                                                <  1:4080749/101‑1 (MQ=255)


          gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..Cg                                                                                               >  1:4137189/1‑101 (MQ=255)


           ggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGc                                                                                              <  2:3999705/101‑1 (MQ=255)


            gggCGTGGATGGAAACACCGTCATCACCACGCAACCGCCCTGCACCGGCGGTAGGTCACCCCGCGGGGGCGTGGATCGAAACAAA.CCGATCATGGC.C..CGcc                                                                                             >  2:4809728/1‑101 (MQ=37)


            gggCGTGGATCGAAACCCCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGcc                                                                                             <  2:5794635/101‑1 (MQ=255)


             ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCt                                                                                            <  1:5395527/101‑1 (MQ=255)


               cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.a                                                                                         <  2:1292565/101‑1 (MQ=255)


               cGTGGATCCAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.a                                                                                         <  1:3395240/101‑1 (MQ=255)


                 tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.Agc                                                                                       <  1:5565396/101‑1 (MQ=255)


                  ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.Agcg                                                                                      <  1:1385918/101‑1 (MQ=255)


                     tCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGcgccg                                                                                   >  2:1021237/1‑101 (MQ=255)


                      cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGcgccgc                                                                                  <  2:5013727/101‑1 (MQ=255)


                       gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCcgcg                                                                                 <  1:2379563/101‑1 (MQ=255)


                        aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCgcgc                                                                                >  2:339084/1‑101 (MQ=255)


                         gctcttccgatctCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCt                                                                               <  2:410508/92‑1 (MQ=38)


                          acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.g                                                                             <  1:1104576/101‑1 (MQ=255)


                           cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.Ga                                                                            >  1:2859937/1‑101 (MQ=255)


                            acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.c                                                                          >  2:2523140/1‑101 (MQ=255)


                             ccGTCATCACCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.Cg                                                                         <  2:3767670/101‑1 (MQ=255)


                             ccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.Cg                                                                         >  1:3803664/1‑101 (MQ=255)


                               gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTc                                                                       >  2:2120496/1‑101 (MQ=255)


                                 catcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGc                                                                     <  1:769372/101‑1 (MQ=255)


                                  atcaCCACGCAACCGCCCTGCACCCCCGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCt                                                                    <  1:1481508/101‑1 (MQ=255)


                                  atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCt                                                                    <  1:3822898/101‑1 (MQ=255)


                                   tcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTc                                                                   <  1:2169665/101‑1 (MQ=255)


                                    cactacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcc                                                                  >  2:4322439/1‑101 (MQ=255)


                                     accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccc                                                                 <  1:3321922/101‑1 (MQ=255)


                                     accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccc                                                                 >  2:290964/1‑101 (MQ=255)


                                     accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccc                                                                 >  1:523486/1‑101 (MQ=255)


                                          gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                            <  2:2355602/101‑1 (MQ=255)


                                           cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                           <  1:4773674/101‑1 (MQ=255)


                                                  ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                    >  2:3666177/1‑101 (MQ=255)


                                                    ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  1:1010202/91‑1 (MQ=2)


                                                     gCACCACCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  2:5194685/101‑1 (MQ=255)


                                                      caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:1679812/101‑1 (MQ=255)


                                                        tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  2:5621194/95‑1 (MQ=0)


                                                        ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  1:2222844/101‑1 (MQ=255)


                                                        ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              >  2:5314202/1‑101 (MQ=255)


                                                         accgcgTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCGATGATCAAATC.C..GCAATC.GGCCGGGCTCG.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  1:1553335/96‑1 (MQ=0)


                                                          cccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            <  2:4689789/100‑1 (MQ=255)


                                                          accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            >  1:1779768/1‑101 (MQ=255)


                                                           tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  2:3320313/98‑1 (MQ=2)


                                                           ctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           >  1:5776906/3‑101 (MQ=2)


                                                           agcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            <  1:2670299/98‑1 (MQ=2)


                                                             ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCGC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                         <  1:929925/101‑1 (MQ=255)


                                                             cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          <  1:82644/100‑1 (MQ=2)


                                                             cgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          <  2:4198967/100‑1 (MQ=2)


                                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                        >  2:1663266/1‑101 (MQ=2)


                                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                        <  2:4417526/101‑1 (MQ=255)


                                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGGGGGGAGCGaaaagggg                                      >  1:3578633/1‑96 (MQ=0)


                                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                       <  1:5110389/101‑1 (MQ=2)


                                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                        <  1:4196028/101‑2 (MQ=2)


                                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                       >  1:1904913/1‑101 (MQ=255)


                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGGTCGaaaaggggc                                     >  1:4491237/1‑95 (MQ=2)


                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                      <  2:137027/101‑2 (MQ=2)


                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                       <  1:2082591/101‑3 (MQ=2)


                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                       >  2:1224800/1‑99 (MQ=2)


                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                    <  1:5590525/101‑7 (MQ=2)


                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                      <  1:2802386/101‑4 (MQ=2)


                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                     >  1:1189406/1‑101 (MQ=255)


                                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgat                                    >  2:3565456/1‑98 (MQ=2)


                                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                     <  2:5069185/101‑5 (MQ=2)


                                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                     >  1:2312889/1‑97 (MQ=2)


                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                  <  2:1792929/101‑9 (MQ=2)


                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCT.GT.C..GGCCGCGAACACCGATAC.CG.CGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgttc                                   >  2:2835365/1‑97 (MQ=2)


                                                                    ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGCCTTGGACCGAAACATCCGCgg                                  >  2:313427/1‑101 (MQ=255)


                                                                     cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                 >  1:433869/1‑95 (MQ=2)


                                                                     cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                 >  1:702922/1‑95 (MQ=2)


                                                                      ccccGCGGGGGCGTTGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                <  2:3852165/101‑1 (MQ=255)


                                                                       cccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                               <  2:550667/101‑1 (MQ=255)


                                                                        ccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                              >  2:4692683/1‑101 (MQ=255)


                                                                        ccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                              <  2:2187822/101‑1 (MQ=255)


                                                                            gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                          >  2:5447002/1‑101 (MQ=255)


                                                                             ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                         <  2:1941575/101‑1 (MQ=255)


                                                                                gCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                      <  2:3090147/101‑1 (MQ=255)


                                                                                 cGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                     <  1:3666405/101‑1 (MQ=255)


                                                                                  ggggATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                    <  2:1492902/99‑1 (MQ=255)


                                                                                  gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                    <  2:4953732/101‑1 (MQ=255)


                                                                                  gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                    <  2:1714259/101‑1 (MQ=255)


                                                                                   tGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                   >  1:4624084/1‑101 (MQ=255)


                                                                                    ggATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccccc                  >  2:723943/1‑101 (MQ=255)


                                                                                    agATCGAAACAAC.CGGATCATGCC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCTGATCGGCGTGAcccgcc                  <  2:3275296/100‑1 (MQ=255)


                                                                                       tCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg               >  1:303692/1‑101 (MQ=255)


                                                                                          aaaCAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc            >  1:371742/1‑101 (MQ=255)


                                                                                          aaaCAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc            >  1:3850196/1‑101 (MQ=255)


                                                                                            acacc.CCGATCAGGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt          <  1:2523282/101‑1 (MQ=255)


                                                                                            acaac.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt          <  2:3822088/101‑1 (MQ=255)


                                                                                            acaac.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt          >  2:1541827/1‑101 (MQ=255)


                                                                                             caac.CCGATCATGGC.C..CGCCTG.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc         >  1:3278616/1‑101 (MQ=255)


                                                                                              aac.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg        >  2:4622675/1‑101 (MQ=255)


                                                                                               ac.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg       >  2:2436555/1‑101 (MQ=255)


                                                                                               ac.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg       <  2:734497/101‑1 (MQ=255)


                                                                                                   cGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGACCCGCCCCGCCCATCGGGCg    >  2:2743840/1‑101 (MQ=255)


                                                                                                   cGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAGCGTCGTGGCACGCCACGCCCCTCGGtag    >  2:888457/1‑99 (MQ=25)


                                                                                                     aTCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt  <  2:2649768/101‑1 (MQ=255)


CTCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCT  >  NC_014034/1397438‑1397638

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG