Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,554 G→A 15.1% S479N (AGC→CAC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5540G→A15.1% 119.5 ‑0.8 ‑0.0311S479N (AGC→AAC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/27):  ref base (132/132):  total (152/159)
Fisher's exact test strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 348 total aligned reads displayed.

GTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1397454‑1397654
                                                                                                          |                                                                                                      
 tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.Agc                                                                                                       <  1:5565396/101‑1 (MQ=255)
   gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.Agcgc                                                                                                     >  1:3710900/1‑101 (MQ=255)
   gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.Agcgc                                                                                                     <  2:3433266/101‑1 (MQ=255)
        aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCgcgc                                                                                                >  2:74260/1‑101 (MQ=255)
         gctcttccgatctCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCt                                                                                               <  2:410508/92‑1 (MQ=38)
          acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.g                                                                                             <  1:1104576/101‑1 (MQ=255)
           cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.Ga                                                                                            >  1:2859937/1‑101 (MQ=255)
           cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.Ga                                                                                            >  2:3625373/1‑101 (MQ=255)
            acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.c                                                                                          >  2:2523140/1‑101 (MQ=255)
             ccGTCATCACCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.Cg                                                                                         <  2:3767670/101‑1 (MQ=255)
              cGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.ctt                                                                                        <  1:3844756/101‑3 (MQ=255)
               gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTc                                                                                       >  2:2120496/1‑101 (MQ=255)
               gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTc                                                                                       >  1:4180537/1‑101 (MQ=255)
                  atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCt                                                                                    >  1:2607650/1‑101 (MQ=255)
                    caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcc                                                                                  <  2:5085233/101‑1 (MQ=255)
                     accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccc                                                                                 >  2:290964/1‑101 (MQ=255)
                        acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                              >  2:901066/1‑101 (MQ=255)
                         cGCAACCGCCCTGCACCCCCGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                             <  2:1346350/101‑1 (MQ=255)
                         cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                             <  1:380966/101‑1 (MQ=255)
                          gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                            <  2:2355602/101‑1 (MQ=255)
                            aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                          >  2:3552075/1‑101 (MQ=255)
                            aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                          >  2:5380486/1‑101 (MQ=255)
                               cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggggg                                                                       >  1:2345018/1‑101 (MQ=255)
                                gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                      <  1:474413/101‑1 (MQ=255)
                                gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                      >  1:796788/1‑101 (MQ=255)
                                gCCCGGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                      <  1:3957730/101‑1 (MQ=255)
                                   cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                   <  2:3712684/101‑1 (MQ=255)
                                    ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  1:1010202/91‑1 (MQ=2)
                                     gCACCACCGGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACAAC.CCAAAAATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  1:4810009/101‑1 (MQ=38)
                                     gCACCACCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  2:5194685/101‑1 (MQ=255)
                                      caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  1:1679812/101‑1 (MQ=255)
                                        tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  2:5621194/95‑1 (MQ=0)
                                         ccccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAA..CTGACCCGGCAC.C..GGCCTA.TGCGCTG.GAC.GT.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            >  1:1842902/3‑101 (MQ=2)
                                         aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            <  2:3980391/96‑1 (MQ=2)
                                         accgcgTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCGATGATCAAA.TC.C..GCAATC.GGCCGGGCTCG.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:1553335/96‑1 (MQ=0)
                                          gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.G.AT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            >  1:1270437/4‑101 (MQ=2)
                                           tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  2:3320313/98‑1 (MQ=2)
                                           agcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            <  1:2670299/98‑1 (MQ=2)
                                            tggTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.G.AT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          <  2:4795059/100‑1 (MQ=2)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                        >  2:1663266/1‑101 (MQ=2)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                        >  2:1055317/1‑101 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCC.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                        <  1:531319/101‑1 (MQ=255)
                                              gTCGCACCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAA.TC.C..GCAATC.GGCCGGGCTCG.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                         <  1:4623812/101‑2 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGGTCGaaaaggggc                                                     >  1:4491237/1‑95 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                      <  2:137027/101‑2 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                       <  1:2082591/101‑3 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                       >  2:4682133/1‑99 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                       <  1:3035318/101‑3 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGGTCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                                     >  1:3286248/1‑99 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                    <  1:5590525/101‑7 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggcg                                                    >  2:2311462/1‑95 (MQ=0)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                      <  2:4518772/101‑4 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGAGGCGTGGATCGAAACaaaa                                                      <  2:3157138/101‑4 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                     >  1:1189406/1‑101 (MQ=255)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                    <  2:4238938/101‑4 (MQ=2)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCT..GT.C..GGCCGCGAACACCGATAC.CG.CGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgttc                                                   >  2:2835365/1‑97 (MQ=2)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                   >  1:5073642/1‑101 (MQ=255)
                                                   cccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                   <  2:5013856/100‑5 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                                  >  1:495962/1‑96 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                   >  2:4355957/1‑95 (MQ=0)
                                                    ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                  >  1:3733821/1‑101 (MQ=255)
                                                     cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                 >  1:702922/1‑95 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                 <  1:3565673/101‑7 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.CGCGCCGCTCT.CA.CGTCGTTCCTCCCGCGGGTGCGTTTATCTAAActcacgaaga                                                 >  2:310365/1‑92 (MQ=21)
                                                       cccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                               >  2:1545276/1‑101 (MQ=255)
                                                        ccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                              >  2:4692683/1‑101 (MQ=255)
                                                          gcgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                            >  1:2243043/1‑101 (MQ=255)
                                                          gcgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                            <  1:1745408/101‑1 (MQ=255)
                                                           cgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                           <  1:1440409/101‑1 (MQ=255)
                                                            gggggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                          <  1:3144757/101‑1 (MQ=255)
                                                             ggggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                         <  2:1941575/101‑1 (MQ=255)
                                                                  ggggATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                    <  2:1492902/99‑1 (MQ=255)
                                                                  gTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                    <  2:1714259/101‑1 (MQ=255)
                                                                   tGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                   >  2:5476236/1‑101 (MQ=255)
                                                                    ggATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccccc                                  >  2:723943/1‑101 (MQ=255)
                                                                     gATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                 >  2:4969/1‑101 (MQ=255)
                                                                       tCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                               >  2:4626226/1‑101 (MQ=255)
                                                                         gAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                             >  1:2196941/1‑101 (MQ=255)
                                                                           aacaac.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                           <  1:3349709/101‑1 (MQ=255)
                                                                            acacc.CCGATCAGG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                          <  1:2523282/101‑1 (MQ=255)
                                                                            acaac.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgcccccccccc                          >  1:4264022/1‑99 (MQ=255)
                                                                            acaac.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                          <  2:3822088/101‑1 (MQ=255)
                                                                               ac.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                       >  2:2436555/1‑101 (MQ=255)
                                                                                   cGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg                    >  2:2663564/1‑101 (MQ=255)
                                                                                     aTCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                  >  2:3248869/1‑101 (MQ=255)
                                                                                     aTCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                  <  2:2649768/101‑1 (MQ=255)
                                                                                        aTG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcct               <  1:5195753/101‑2 (MQ=255)
                                                                                        aTG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc               >  1:3611044/1‑101 (MQ=255)
                                                                                        aTG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc               >  1:97823/1‑101 (MQ=255)
                                                                                         tG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccc              <  1:339128/101‑1 (MQ=255)
                                                                                          g.gC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc            <  1:873876/101‑1 (MQ=255)
                                                                                             gac..cGCCGC.AGCGCCGCGCT.GA.CGACGCACCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc            <  1:2794470/99‑1 (MQ=255)
                                                                                             c.c..cGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg           <  1:243880/101‑1 (MQ=255)
                                                                                                cactgcTC.ACCGCCGCGCG.AG.CGTGTCTCCACCCGCGGGGGCGTCGACCGAAACTTCCGCAGATTGGCTTTTCCCGCCCCGCCCATCGGCCGCTCCCCCcg           <  2:1821172/96‑1 (MQ=255)
                                                                                                  cGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg         <  2:3847102/101‑1 (MQ=255)
                                                                                                  cGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg         <  1:2144085/101‑1 (MQ=255)
                                                                                                     cTC.AGCGCCGCGCG.GA.CGTCGCCCCCCCCGCGGGGGGGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg      <  1:5780207/101‑1 (MQ=38)
                                                                                                      tC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg     >  2:5792056/1‑101 (MQ=255)
                                                                                                       c.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc    <  2:4180287/101‑1 (MQ=255)
                                                                                                         aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg   >  2:868676/1‑101 (MQ=255)
                                                                                                          |                                                                                                      
GTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1397454‑1397654

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG