Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,398,006 C→T 1.6% intergenic (+310/+1588) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,398,0060C→T 1.6% 2.5 0.0 ‑0.2252intergenic (+310/+1588)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (131/117):  total (133/119)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.50e-01

Only 100 of 284 total aligned reads displayed.

GCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCT  >  NC_014034/1397906‑1398106
                                                                                                    |                                                                                                    
gCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccgcc                                                                                                      <  2:5649328/101‑1 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccgccg                                                                                                     >  1:4045135/1‑101 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccgccg                                                                                                     <  1:341574/101‑1 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccgccg                                                                                                     >  2:3599690/1‑101 (MQ=255)
     ccccGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCa                                                                                                 <  2:1800168/101‑1 (MQ=255)
      cccGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCaa                                                                                                <  1:1940294/101‑1 (MQ=255)
         gcgGGGGCGTGGATCGAATCTCTATGGGAGGCTAGGGAGTCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGGGGATCTAAACCGCCGCGCaaaaa                                                                                             <  2:977202/101‑1 (MQ=38)
         gcgGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCaaaaa                                                                                             >  2:4833621/1‑101 (MQ=255)
         gcgGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCaaaaa                                                                                             >  2:2720047/1‑101 (MQ=255)
         gcgGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCaaaaa                                                                                             <  2:4671130/101‑1 (MQ=255)
          cgGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCCAAAAg                                                                                            >  2:5504940/1‑101 (MQ=255)
             gggCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGccc                                                                                         <  1:3970796/101‑1 (MQ=255)
             gggCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGccc                                                                                         <  1:3625949/101‑1 (MQ=255)
              ggCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCg                                                                                        <  2:4533113/101‑1 (MQ=255)
                cGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGcc                                                                                      >  1:4334448/1‑101 (MQ=255)
                 gTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCa                                                                                     >  1:5667467/1‑101 (MQ=255)
                 gTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCa                                                                                     >  2:5403013/1‑101 (MQ=255)
                 gTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCa                                                                                     <  1:3072198/101‑1 (MQ=255)
                  tGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCaa                                                                                    >  1:4157080/1‑101 (MQ=255)
                  tGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCaa                                                                                    >  2:2784871/1‑101 (MQ=255)
                   ggATCGAAACTCTATGGGAGGCTAGGGGGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCCCCaag                                                                                   >  1:926616/1‑101 (MQ=255)
                   ggATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCaag                                                                                   <  2:3755478/101‑1 (MQ=255)
                   ggATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCaag                                                                                   >  1:4225188/1‑101 (MQ=255)
                       cGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGc                                                                               <  2:1062787/101‑1 (MQ=255)
                       cGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGc                                                                               >  1:2115356/1‑101 (MQ=255)
                        gAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCg                                                                              >  2:5597232/1‑101 (MQ=255)
                        gAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCg                                                                              >  2:2105395/1‑101 (MQ=255)
                          aaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGa                                                                            <  1:4466876/101‑1 (MQ=255)
                          aaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGa                                                                            >  1:1776610/1‑101 (MQ=255)
                           aCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAg                                                                           <  1:1152488/101‑1 (MQ=255)
                           aCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAg                                                                           <  1:5101804/101‑1 (MQ=255)
                             tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCa                                                                         >  2:2542963/1‑101 (MQ=255)
                             tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCa                                                                         >  2:4736212/1‑101 (MQ=255)
                              ctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAgatc                                                                        >  2:1969495/1‑98 (MQ=255)
                              ctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCaa                                                                        <  1:2801143/101‑1 (MQ=255)
                               tATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAg                                                                       >  1:3864434/1‑101 (MQ=255)
                               tATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAg                                                                       <  2:1197715/101‑1 (MQ=255)
                               tATGGGAGCCTAGGGAAGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAg                                                                       <  2:4437200/101‑1 (MQ=255)
                                aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGt                                                                      >  1:854999/1‑101 (MQ=255)
                                aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGt                                                                      <  1:1799828/101‑1 (MQ=255)
                                 tGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTc                                                                     >  1:2029551/1‑101 (MQ=255)
                                       gctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTccc                                                               <  2:1939131/101‑1 (MQ=255)
                                       gctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTccc                                                               >  2:157567/1‑101 (MQ=255)
                                          agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTcccccc                                                            >  1:766962/1‑101 (MQ=255)
                                          agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTcccccc                                                            <  2:402247/101‑1 (MQ=255)
                                          agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTcccccc                                                            <  1:735557/101‑1 (MQ=255)
                                           gggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCcg                                                           >  1:160378/1‑101 (MQ=255)
                                            ggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCcgc                                                          >  2:5687864/1‑101 (MQ=255)
                                              gggctaCGCCCCTTCAAAAGTCGCTCCCCCCGCGGGGCCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCgg                                                        <  2:1367250/100‑1 (MQ=255)
                                               ggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCggg                                                       <  1:1511433/101‑1 (MQ=255)
                                                 ctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCggggg                                                     >  2:439287/1‑101 (MQ=255)
                                                  taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGc                                                    >  1:5700832/1‑101 (MQ=255)
                                                  taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGc                                                    >  1:3962461/1‑101 (MQ=255)
                                                     gccccTTCAAAAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGCCGCGTAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCCGGGGAGTg                                                 >  2:3477933/4‑101 (MQ=37)
                                                     gCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTg                                                 <  1:4736493/101‑1 (MQ=255)
                                                      cACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                <  1:4677303/101‑1 (MQ=255)
                                                       aCCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                               >  2:5214270/1‑101 (MQ=255)
                                                        ccTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                              >  2:312/1‑101 (MQ=255)
                                                        ccTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  2:5667172/101‑1 (MQ=255)
                                                        ccTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  1:4828444/101‑1 (MQ=255)
                                                         cTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:3429610/101‑1 (MQ=255)
                                                            cAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTa                                          >  2:5140408/1‑101 (MQ=255)
                                                             aaaaGCCGCTCCCCCCGCGGGGGCGTGGATCAAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTaa                                         <  1:1062868/101‑1 (MQ=255)
                                                               aaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGc                                       >  2:3512476/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCgg                                     >  2:3298320/1‑101 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGATCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTg                                   >  1:2579662/1‑101 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTg                                   >  2:5769197/1‑101 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTg                                   >  2:796321/1‑101 (MQ=255)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCa                                 >  1:3250909/1‑101 (MQ=255)
                                                                      cccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCGAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                                <  2:3980517/100‑1 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATc                               >  2:2456415/1‑101 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCg                              <  2:4224928/101‑1 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCgg                             >  1:2646138/1‑101 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCgg                             <  2:2035382/101‑1 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGc                            >  2:5083289/1‑101 (MQ=255)
                                                                           ccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGcc                           >  2:2147088/1‑101 (MQ=255)
                                                                            cgcgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGccc                          <  1:5136228/101‑1 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAACCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCg                         <  1:1024253/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                       <  1:5403303/101‑1 (MQ=255)
                                                                                gtgtcGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATa                      <  2:5531245/97‑1 (MQ=255)
                                                                                ggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATa                      <  1:5519522/101‑1 (MQ=255)
                                                                                ctggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATa                      <  1:1240763/99‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaa                     <  1:4423123/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaa                     <  2:3025197/101‑1 (MQ=255)
                                                                                  ggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaaa                    <  1:2296165/101‑1 (MQ=255)
                                                                                      tGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAcccc                <  2:5287733/101‑1 (MQ=255)
                                                                                      tGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAcccc                <  1:5597524/101‑1 (MQ=255)
                                                                                       ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg               >  2:1845163/1‑101 (MQ=255)
                                                                                        gATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGa              >  1:3171510/1‑101 (MQ=255)
                                                                                        gATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGa              >  1:2389469/1‑101 (MQ=255)
                                                                                           cGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAttt           <  2:116135/101‑1 (MQ=255)
                                                                                            gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtttt          <  2:221605/101‑1 (MQ=255)
                                                                                            gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtttt          >  1:2447907/1‑101 (MQ=255)
                                                                                            gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAAGCGGTGCATCGGCCCGATAAAACCCGAtttt          >  1:4931815/1‑101 (MQ=255)
                                                                                             aaaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTc         <  2:4782296/101‑1 (MQ=255)
                                                                                             aaaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTc         <  1:2168401/101‑1 (MQ=255)
                                                                                              acccgccgCCCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg        <  1:2902967/99‑1 (MQ=255)
                                                                                              aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAATTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAttttag        <  2:2962563/101‑3 (MQ=255)
                                                                                              aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg        >  1:5224609/1‑101 (MQ=255)
                                                                                                 cgccgcGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGcc     <  2:4691024/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCT  >  NC_014034/1397906‑1398106

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG