Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,405 C→G 16.3% intergenic (‑184/‑774) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4050C→G16.3% 148.2 ‑1.3 ‑0.0375intergenic (‑184/‑774)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (35/26):  ref base (153/160):  total (189/186)
Fisher's exact test strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.92e-01

Only 100 of 388 total aligned reads displayed.

TGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1434305‑1434505
                                                                                                       |                                                                                                      
tGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGc                                                                                                        <  1:5607250/101‑1 (MQ=255)
 ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCg                                                                                                       <  2:678645/101‑1 (MQ=255)
 ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCg                                                                                                       >  2:5249268/1‑101 (MQ=255)
   aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTc                                                                                                     <  2:2156840/101‑1 (MQ=255)
    tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCGCTGCTTCACG.GCTGCGTCa                                                                                                    <  1:2760109/101‑1 (MQ=255)
     cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCaa                                                                                                   <  1:4029698/101‑1 (MQ=255)
       aaaCCGGATGCTTGGGCCGGAACCCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAAcc                                                                                                 <  2:2727205/101‑1 (MQ=37)
           aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..Gt                                                                                           >  2:4399114/1‑101 (MQ=255)
           aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..Gt                                                                                           >  2:1063893/1‑101 (MQ=255)
             gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCg                                                                                         <  1:2726376/101‑1 (MQ=255)
                 ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcc                                                                                     >  1:4836345/1‑101 (MQ=255)
                  tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTccc                                                                                    <  2:1854095/101‑1 (MQ=255)
                  tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTccc                                                                                    >  1:4382551/1‑101 (MQ=255)
                   ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccc                                                                                   >  1:3605684/1‑101 (MQ=255)
                     gggCGGAACACCTGCTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccccc                                                                                 <  2:598040/101‑1 (MQ=255)
                     gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccccc                                                                                 >  2:1705960/1‑101 (MQ=255)
                       gCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCcgc                                                                               <  2:965104/101‑1 (MQ=255)
                        cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCcgcg                                                                              >  1:1395533/1‑101 (MQ=255)
                         ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccccccgcg                                                                             >  2:4877709/1‑97 (MQ=255)
                         ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCgg                                                                             >  1:5695566/1‑101 (MQ=255)
                         ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCgg                                                                             >  2:1010381/1‑101 (MQ=255)
                          gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCggg                                                                            >  1:3739986/1‑101 (MQ=255)
                           aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCgggg                                                                           <  2:2097317/101‑1 (MQ=255)
                           aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCgggg                                                                           <  2:3115403/101‑1 (MQ=255)
                            acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCggggg                                                                          >  2:220893/1‑101 (MQ=255)
                             ccccTGATCCGTCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                                         <  2:1112492/99‑1 (MQ=255)
                             cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                                         >  1:4101914/1‑101 (MQ=255)
                                 tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  2:2944004/1‑101 (MQ=255)
                                 tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  1:3494887/101‑1 (MQ=255)
                                   gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTg                                                                      <  1:1832677/91‑1 (MQ=2)
                                   aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  1:1602863/101‑1 (MQ=255)
                                    cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  2:1299263/10‑101 (MQ=2)
                                     gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                    <  2:2475549/93‑1 (MQ=2)
                                     gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                    >  1:3987647/9‑101 (MQ=2)
                                     ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  1:471570/1‑101 (MQ=255)
                                      ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  1:606782/94‑1 (MQ=2)
                                      ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGagcca                                                                >  2:453850/1‑97 (MQ=255)
                                       tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               >  2:4606055/1‑101 (MQ=255)
                                       tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               <  1:284449/101‑1 (MQ=255)
                                       gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:3021744/95‑1 (MQ=2)
                                        gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              >  1:769565/1‑101 (MQ=255)
                                        gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              <  2:740808/101‑1 (MQ=255)
                                        ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGAATTCGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGGTCg                                                                 >  1:4955797/6‑101 (MQ=0)
                                         ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                             <  1:2589283/101‑1 (MQ=255)
                                           gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                           <  2:267366/101‑1 (MQ=255)
                                           gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                           >  2:4097125/1‑101 (MQ=255)
                                            cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                             >  1:4583036/2‑101 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                         <  2:5350320/101‑1 (MQ=255)
                                             gTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                         <  1:2338665/101‑1 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                          >  1:1493945/1‑98 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                          >  2:4740812/1‑98 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                                       >  2:5423719/1‑101 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                                       <  1:4468047/101‑1 (MQ=255)
                                                gccccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGTCCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                         <  1:1782793/98‑5 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                         >  2:576212/1‑97 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                      >  1:95218/1‑101 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                        <  1:3212315/101‑6 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAACGGATTg                                                     >  2:2844339/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                       >  2:4479162/1‑95 (MQ=2)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                    <  2:3313468/101‑1 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                    >  2:831607/1‑101 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                                     >  2:4382530/1‑93 (MQ=2)
                                                    cccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                                     <  1:1726752/101‑9 (MQ=2)
                                                    cccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                                     <  1:4502407/101‑9 (MQ=2)
                                                     ccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                                                    <  2:5138694/101‑10 (MQ=2)
                                                     ccccGCGGGGGCGTGGATCGAAAC..TTGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                  <  1:310398/101‑8 (MQ=37)
                                                     ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                                 >  2:1933485/1‑101 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                                 <  1:4336765/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                                   >  1:2076580/1‑91 (MQ=0)
                                                       ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                               >  2:198958/1‑101 (MQ=255)
                                                          cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                            <  1:1191759/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGACGAgg                                        >  2:2512421/1‑101 (MQ=255)
                                                               gCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                                       >  1:5070344/1‑101 (MQ=255)
                                                                cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                      >  2:2245623/1‑101 (MQ=255)
                                                                cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                      <  2:3802414/101‑1 (MQ=255)
                                                                 gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                     <  1:5342668/101‑1 (MQ=255)
                                                                  tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                    <  1:2944171/101‑1 (MQ=255)
                                                                   ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                   >  2:1457480/1‑101 (MQ=255)
                                                                       cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                               >  2:153626/1‑101 (MQ=255)
                                                                       cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                               <  2:5634958/101‑1 (MQ=255)
                                                                          aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                            <  1:2440264/101‑1 (MQ=255)
                                                                           aC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                           <  2:1226789/101‑1 (MQ=255)
                                                                            c..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                          >  1:3259286/1‑101 (MQ=255)
                                                                                tctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                        >  2:2348026/1‑101 (MQ=255)
                                                                                tctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                        <  1:5516311/101‑1 (MQ=255)
                                                                                 ctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg                       <  2:2783174/101‑1 (MQ=255)
                                                                                    cGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc                    <  1:2010432/101‑1 (MQ=255)
                                                                                     gCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                   <  2:162664/101‑1 (MQ=255)
                                                                                      ctctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccc                  <  1:5176175/101‑1 (MQ=255)
                                                                                        ctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                <  2:348944/101‑1 (MQ=255)
                                                                                         tgattCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc               >  1:3295445/4‑101 (MQ=255)
                                                                                         tGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc               <  1:1031937/101‑1 (MQ=255)
                                                                                         tGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc               <  2:3913419/101‑1 (MQ=255)
                                                                                          cttccgatctcTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc               <  1:3318219/91‑1 (MQ=255)
                                                                                           cTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgc             >  1:2250087/1‑101 (MQ=255)
                                                                                           cTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGCGCGTGGATCGAAACACGGATTCCAGGGCGCCGCGGGACAGGCTTTGCAGTCCCTcccccccc             >  1:5654308/1‑99 (MQ=17)
                                                                                                cG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCggggg        <  2:421363/101‑1 (MQ=255)
                                                                                                 g.gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc       >  2:5047372/1‑101 (MQ=40)
                                                                                                     tGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTg    >  1:3898205/1‑101 (MQ=38)
                                                                                                      gCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg   <  2:383946/101‑1 (MQ=37)
                                                                                                       |                                                                                                      
TGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1434305‑1434505

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG