Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,668,401	G→C	100%	intergenic (‑66/+16)	ilvH ← / ← ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,668,401	0	G→C	100.0%	106.4	30	intergenic (‑66/+16)	ilvH/ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (28/1):  ref base (1/0):  total (29/1)

CCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGG  >  NC_014034/1668310‑1668490                                                                                            |                                                                                          ccTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GGGCCCCCCgccg                                                                                >  2:3511040/1‑98 (MQ=255)    ttCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGct                                                                             >  1:2024822/1‑101 (MQ=255)     tCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGctc                                                                            >  2:3074591/1‑101 (MQ=255)             ttCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCa                                                                    >  1:2559777/1‑101 (MQ=255)              tCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCAAACCAg                                                                   >  1:4829571/1‑101 (MQ=25)                 gagCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAgcgc                                                                >  1:121094/1‑101 (MQ=255)                  agCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGcgcg                                                               >  1:2820764/1‑101 (MQ=255)                         aTTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCC.GGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGcgccgcc                                                       >  1:2108982/1‑101 (MQ=37)                                             aCTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCg                                    >  1:5633883/1‑101 (MQ=255)                                                   ttATCTTGCCGGAAATATCCCGGGGGGCCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCCCGGCATCGGCCgag                              >  2:1929519/1‑101 (MQ=25)                                                    tATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCgagg                             >  1:2413141/1‑100 (MQ=37)                                                     aTCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGagag                            >  2:2003161/1‑101 (MQ=255)                                                        ttGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGagcag                         >  1:3782248/1‑101 (MQ=255)                                                          gCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAgcagca                       >  1:2273780/1‑101 (MQ=255)                                                                 atatCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGtt                >  1:982820/1‑101 (MQ=255)                                                                   atCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTAt              >  1:2608608/1‑101 (MQ=255)                                                                     cccGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGc            >  2:1919481/1‑101 (MQ=255)                                                                        ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc         >  2:954930/1‑101 (MQ=255)                                                                        ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc         >  1:1514520/1‑101 (MQ=255)                                                                          gatctCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCtt       <  2:75965/97‑1 (MQ=255)                                                                           gggTCCGGGGGGCA..GCGCCCCCCGGGCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTg      >  1:3630984/1‑101 (MQ=255)                                                                           gggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTg      >  1:407908/1‑101 (MQ=255)                                                                           gggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTg      >  2:4816993/1‑101 (MQ=255)                                                                            ggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGc     >  1:1752743/1‑101 (MQ=255)                                                                            ggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGc     >  1:152163/1‑101 (MQ=255)                                                                             gTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGcc    >  1:50634/1‑101 (MQ=255)                                                                             gTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGcc    >  1:5276300/1‑101 (MQ=255)                                                                              tCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCg   >  1:2163291/1‑101 (MQ=255)                                                                               ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCttagatc  >  1:75976/1‑96 (MQ=255)                                                                               ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  1:3315519/1‑101 (MQ=255)                                                                               ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  1:2188646/1‑101 (MQ=255)                                                                               ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  2:5584810/1‑101 (MQ=255)                                                                                            |                                                                                          CCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGG  >  NC_014034/1668310‑1668490 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG