Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,166 A→C 100% intergenic (‑94/+131) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1660A→C100.0% 924.6 278intergenic (‑94/+131)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (127/151):  ref base (0/0):  total (127/151)

Only 100 of 286 total aligned reads displayed.

AATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTT.CG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCG  >  NC_014034/1696066‑1696266
                                                                                                    |                                                                                                       
aaTCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTg                                                                                                       <  1:2546970/101‑1 (MQ=255)
  tCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGc                                                                                                     <  1:2006847/101‑1 (MQ=255)
    cATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCt                                                                                                   <  1:2947284/101‑1 (MQ=255)
    cATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCt                                                                                                   <  1:2362370/101‑1 (MQ=255)
    cATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCt                                                                                                   <  1:2287293/101‑1 (MQ=255)
     aTGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGccct                                                                                                  >  2:1667540/1‑99 (MQ=255)
     aTGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCtt                                                                                                  <  2:3402278/101‑1 (MQ=255)
     aTGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCtt                                                                                                  >  2:5374362/1‑101 (MQ=255)
        cgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.Cg                                                                                              <  1:3062424/101‑1 (MQ=255)
        cgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.Cg                                                                                              <  2:1449006/101‑1 (MQ=255)
         gcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.Cg.g                                                                                            >  1:5454871/1‑101 (MQ=255)
         gcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.CGCCCCTCCTGGCCTTT.Cg.g                                                                                            >  2:1961233/1‑101 (MQ=255)
           tcctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.cc                                                                                         >  1:666480/1‑100 (MQ=255)
           tcctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.cc                                                                                         >  1:1624267/1‑100 (MQ=255)
              tccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.ccaat                                                                                      >  1:4489797/1‑97 (MQ=255)
              tccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.ccaat                                                                                      <  2:4530900/101‑5 (MQ=255)
                ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTc                                                                                     >  1:1727383/1‑101 (MQ=255)
                   aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAcc                                                                                 >  2:5272432/1‑101 (MQ=255)
                    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAccc                                                                                >  1:2403507/1‑101 (MQ=255)
                    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAccc                                                                                >  2:2900889/1‑101 (MQ=255)
                    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCCTT.CG.G.cccattcaccc                                                                                >  2:1110872/1‑91 (MQ=255)
                     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAcccc                                                                               <  2:3635451/101‑2 (MQ=37)
                     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAcccc                                                                               <  1:935325/101‑2 (MQ=37)
                     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAcccc                                                                               >  1:5060482/1‑100 (MQ=255)
                     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAcccc                                                                               >  2:3132424/1‑100 (MQ=255)
                      tGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.G.CCAATTCAccccg                                                                              <  1:1151131/101‑3 (MQ=255)
                          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCggcat                                                                           <  1:3298167/101‑4 (MQ=255)
                           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatg                                                                          >  2:3510539/1‑99 (MQ=255)
                           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatg                                                                          >  2:4697478/1‑99 (MQ=255)
                           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatg                                                                          >  1:1668672/1‑99 (MQ=255)
                           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatg                                                                          >  2:5656032/1‑99 (MQ=255)
                            aaTTTATCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGTCGC.TCCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGatgt                                                                         <  2:4616024/101‑4 (MQ=255)
                               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTg                                                                      >  2:1485453/1‑101 (MQ=255)
                               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTg                                                                      >  2:5338113/1‑101 (MQ=255)
                               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTg                                                                      >  1:1759181/1‑101 (MQ=255)
                                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGGGGGGC.GC.TACCCCTCCCGGCCTTTCCG.C.CAATTCACCCCGGGATGTTTgg                                                                     >  1:3534306/1‑101 (MQ=255)
                                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTgg                                                                     >  1:4804312/1‑101 (MQ=25)
                                 aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTggg                                                                    >  1:5725083/1‑101 (MQ=255)
                                  aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGc                                                                   <  2:2068822/101‑1 (MQ=255)
                                   cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGcc                                                                  >  2:1045481/1‑101 (MQ=25)
                                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                 >  2:9278/1‑101 (MQ=255)
                                     tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaa                                                                <  2:4319786/101‑1 (MQ=255)
                                      cTGTTTAATTTCGAATCCTTGCACGACTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                               <  1:3478717/101‑2 (MQ=255)
                                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                               >  2:2167766/1‑100 (MQ=21)
                                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                               >  2:2596198/1‑100 (MQ=21)
                                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                               <  1:4130743/101‑2 (MQ=255)
                                        gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                             >  1:4290886/1‑98 (MQ=255)
                                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                            <  1:3778367/101‑5 (MQ=255)
                                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                           <  1:1214201/101‑6 (MQ=16)
                                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                           >  2:1008630/1‑96 (MQ=255)
                                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaagttgaa                                                          <  2:4898935/101‑7 (MQ=255)
                                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                          >  1:2666089/1‑95 (MQ=255)
                                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                          <  1:5644703/101‑7 (MQ=255)
                                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                         >  1:3744567/1‑94 (MQ=16)
                                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                      >  2:4582163/1‑101 (MQ=255)
                                              tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                     >  1:5313002/1‑100 (MQ=255)
                                              tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                     >  1:1408478/1‑100 (MQ=255)
                                              tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                     >  2:1849712/1‑100 (MQ=255)
                                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                 <  2:2394705/101‑6 (MQ=255)
                                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                 >  2:2250233/1‑96 (MQ=12)
                                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGCC.CAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                >  2:382282/1‑95 (MQ=255)
                                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                <  2:425801/101‑7 (MQ=12)
                                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCGAA..GGTGAAacaccttgc                                               >  1:4711172/1‑94 (MQ=255)
                                                     tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                              <  2:4404361/101‑9 (MQ=255)
                                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                             <  2:4833660/101‑10 (MQ=9)
                                                       cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                            >  1:5080158/1‑91 (MQ=255)
                                                          gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                       <  2:1598833/101‑1 (MQ=255)
                                                          gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                       <  2:3555451/101‑1 (MQ=255)
                                                           cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                      <  1:2209872/101‑1 (MQ=255)
                                                           cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                      <  1:289385/101‑1 (MQ=255)
                                                            cgatcttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                     <  1:1522735/96‑1 (MQ=2)
                                                            aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                     >  2:3291777/1‑101 (MQ=255)
                                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                    >  1:101160/1‑101 (MQ=255)
                                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                    <  2:2720343/101‑1 (MQ=255)
                                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                    <  1:5175985/101‑1 (MQ=255)
                                                              gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                   >  1:2460034/1‑101 (MQ=9)
                                                                  tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                               <  1:1568370/101‑1 (MQ=9)
                                                                    tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                             >  1:4960620/1‑101 (MQ=9)
                                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                            <  2:667129/101‑1 (MQ=9)
                                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                          >  2:4138663/1‑101 (MQ=9)
                                                                          gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                       >  1:390964/1‑101 (MQ=9)
                                                                          gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                       <  1:4582439/101‑1 (MQ=255)
                                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCCCCCCGGGATGTTTTGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATCCTCct                      >  1:792507/1‑101 (MQ=255)
                                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                      <  1:3157774/101‑1 (MQ=9)
                                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                    <  2:1376288/101‑1 (MQ=255)
                                                                                  agaggggcgctGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                 <  2:286209/91‑1 (MQ=255)
                                                                                   gaggggcgctGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGATTTTTCTTGGCGCAAATACGCCTCTGGcc                >  2:5422282/10‑101 (MQ=255)
                                                                                      gggcgctGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa             <  2:761079/95‑1 (MQ=12)
                                                                                       ggcgctGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa            <  2:817268/96‑1 (MQ=255)
                                                                                        gcgctGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg           <  1:411131/97‑1 (MQ=16)
                                                                                         cgctGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg          <  2:4064968/98‑1 (MQ=16)
                                                                                          gctGCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg         >  1:852627/3‑101 (MQ=16)
                                                                                             gCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCGTCTGGCCCGAAGGGCaa      >  2:5195249/1‑101 (MQ=255)
                                                                                             gCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa      <  1:5786815/101‑1 (MQ=255)
                                                                                             gCCCCTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa      <  1:2856284/101‑1 (MQ=255)
                                                                                              ccccTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg     <  1:1485546/101‑1 (MQ=255)
                                                                                              ccccTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaac     >  1:2721148/1‑100 (MQ=16)
                                                                                              ccccTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg     <  1:5344937/101‑1 (MQ=255)
                                                                                               cccTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAACGGCAAGc    <  2:1178357/101‑1 (MQ=16)
                                                                                                ccTCCTGGCCTTT.CG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc   <  2:2459900/101‑1 (MQ=255)
                                                                                                    |                                                                                                       
AATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTT.CG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCG  >  NC_014034/1696066‑1696266

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG